BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,747,149	G→T	6.3%	V7L (GTG→TTG)	ydhS →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,747,149	0	G	T	6.3%	374.6 / 3.3	119	V7L (GTG→TTG)	ydhS	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (54/53); new base T (2/7); total (59/60)
Fisher's exact test for biased strand distribution p-value = 1.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGG  >  NC_000913/1747001‑1747293

ccTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATtg                                                                                                                                                  <  6:58878/149‑1 (MQ=255)

   gACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTTTgg                                                                                                                                               >  6:122006/1‑149 (MQ=255)

          tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATtgt                                                                                                                                                 >  7:189559/1‑140 (MQ=255)

          tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATtgt                                                                                                                                                 <  8:189559/140‑1 (MQ=255)

          tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                                                                                                                                        <  7:244934/149‑1 (MQ=255)

          tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                                                                                                                                        >  8:460147/1‑149 (MQ=255)

          tttAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCgg                                                                                                                                        <  6:329647/149‑1 (MQ=255)

           ttAATCAAGGGCGGTTAGCGCCCTTTTCATCACTGTCTGAAATTTCTAAAATTCTAAAAATATCAACAAAACTTATATGATAACACTAAATTCGAAAGAATTCGTACAGGTAAGTAACAATGAAAAAAATAGCTAATGTCGGTGCAgg                                                                                                                                        >  1:61885/1‑148 (MQ=255)

             aaTCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTGCAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                      <  1:159137/148‑1 (MQ=255)

               tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGCAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                   >  4:82649/1‑149 (MQ=255)

               tCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTa                                                                                                                                   >  4:94209/1‑149 (MQ=255)

                  aGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                <  2:394779/149‑1 (MQ=255)

                   gggCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACggg                                                                                                                               <  1:360698/149‑1 (MQ=255)

                       ggTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATc                                                                                                                           <  3:172795/149‑1 (MQ=255)

                           aGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTaca                                                                                                                       >  4:322957/1‑149 (MQ=255)

                           aGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTac                                                                                                                        <  7:171564/148‑1 (MQ=255)

                            gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                            <  6:260828/143‑1 (MQ=255)

                            gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGAt                                                                                                                            >  5:260828/1‑143 (MQ=255)

                            gcgcCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTacac                                                                                                                      <  7:94639/149‑1 (MQ=255)

                                ccTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTa                                                                                                                  <  2:161145/149‑1 (MQ=255)

                                      cATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATtctc                                                                                                             >  2:363639/1‑148 (MQ=255)

                                          ccTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTc                                                                                                        <  1:363639/149‑1 (MQ=255)

                                          ccTGGCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTTTTTTTTTGTTTCCGGGACTACGGTGATCTACACCTTATTCTCGCTTc                                                                                                        <  2:123220/149‑1 (MQ=255)

                                              tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACa                                                                                                    <  1:35194/149‑1 (MQ=255)

                                              tCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTa                                                                                                      <  5:122006/147‑1 (MQ=255)

                                                tGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGATTGCGTACAGGTTAGTAACATTGAATAAAATTGCTATTGTGGGTGCGGGGCCTACGGGGATCTAGACCTTATTCTCGCTTCTCCAg                                                                                                   >  3:375076/1‑148 (MQ=255)

                                                 gAAATTTCTCAAATTCTAAAAATCTCAACCAACCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTGATTCTCGCTTCTCCAGc                                                                                                  >  6:364849/1‑148 (MQ=255)

                                                 gAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                      <  7:211256/112‑1 (MQ=255)

                                                 gAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGc                                                                                                                                      >  8:211256/1‑112 (MQ=255)

                                                 gAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGc                                                                                                  >  4:401577/1‑148 (MQ=255)

                                                    agttCTCAAATTAAAAAAATCTCACCCACACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAATTTTTTTTTTTATGATTCGGGGACTACGGTGATCTACACCTTATtct                                                                                                              <  2:243361/131‑1 (MQ=255)

                                                    aTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaac                                                                                               >  6:174165/1‑148 (MQ=255)

                                                    aTTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGcaac                                                                                               >  4:241477/1‑148 (MQ=255)

                                                        ctcAAATTCTAAAAATCTCAACCAAACTTATCTTATAACACTAAATTCGACAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGTATCTACACCTTATTCTCGCTTCTACAGaaacaaac                                                                                           <  3:64032/148‑1 (MQ=255)

                                                         tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                <  3:235813/110‑1 (MQ=255)

                                                         tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                >  4:235813/1‑110 (MQ=255)

                                                         tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                         >  8:269192/1‑149 (MQ=255)

                                                         tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                         >  4:96336/1‑149 (MQ=255)

                                                         tcAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTCTCGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                         >  1:403569/1‑149 (MQ=255)

                                                          cAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                         >  4:312538/1‑148 (MQ=255)

                                                           aaaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAATTAACAATGAAAAATATTGTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                       <  2:134657/149‑1 (MQ=255)

                                                           aaaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGTGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTc                                                                                         <  3:96336/147‑1 (MQ=255)

                                                           aaaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                       <  3:401577/149‑1 (MQ=255)

                                                           aaaTTCTAAAAATCTCAACAAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTTTGGGAGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCa                                                                                       >  1:221029/1‑149 (MQ=255)

                                                            aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTa                                                                                                                         <  3:417689/114‑1 (MQ=255)

                                                            aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCAc                                                                                      <  3:322957/149‑1 (MQ=255)

                                                            aaTTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATCCGTACACGTAAGTAACAATCAAAAAAATTGCTATTGAGGCTGCCGGGCCTACGGGGATCTa                                                                                                                         >  4:417689/1‑114 (MQ=255)

                                                                 tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACt                                                                                     >  5:305290/1‑145 (MQ=255)

                                                                 tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGTTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACt                                                                                     <  6:305290/145‑1 (MQ=255)

                                                                 tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGAGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACttac                                                                                  >  7:469742/1‑146 (MQ=255)

                                                                 tAAAAATCTCAACCAAACTTATCTGATAACACTAAATTAGAAAGAATGATTACAGGTAAGTAACAATGAAAAAAATTGCTTTTGTGGGTGCCGGGCCTACGGGGATCTACACCTAATTCTCGCTTCTACATCAACAAAATCCACattca                                                                                 >  6:366350/1‑148 (MQ=255)

                                                                  aaaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                <  8:38495/101‑1 (MQ=255)

                                                                  aaaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                >  7:38495/1‑101 (MQ=255)

                                                                   aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctat                                                                               >  6:207755/1‑149 (MQ=255)

                                                                   aaaaTCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttct                                                                                 <  5:174165/147‑1 (MQ=255)

                                                                            aCCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCtt                                                                      <  1:65802/149‑1 (MQ=255)

                                                                             ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                <  1:235944/90‑1 (MQ=255)

                                                                             ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACgg                                                                                                                                >  2:235944/1‑90 (MQ=255)

                                                                             ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttc                                                                                  >  2:53602/1‑136 (MQ=255)

                                                                             ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttc                                                                                  <  1:53602/136‑1 (MQ=255)

                                                                             ccAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                     <  3:312538/149‑1 (MQ=255)

                                                                               aaaCTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                     >  1:211729/1‑147 (MQ=255)

                                                                                 aCTTATCTGAAAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGTAAAAATTTTTTATTTTTGTTTCGGGGACGACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGc                                                                 <  2:336538/149‑1 (MQ=255)

                                                                                     aTCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttct                                                                           <  7:184772/135‑1 (MQ=255)

                                                                                         gATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                         >  4:42609/1‑149 (MQ=255)

                                                                                          aTAACACTAAATTCGAAAGAATGCGTACAGGTCAGTAACAATGAAAATATTTTGTTTTGTGGGTGCGGGGCCTACGGGGATCTACACCTTATTCTAGCTTCTACAGCAACAAACTCAACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                         <  8:469742/148‑1 (MQ=255)

                                                                                          aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                         >  8:215156/1‑148 (MQ=255)

                                                                                          aTAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAc                                                         <  8:170094/148‑1 (MQ=255)

                                                                                           tAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGCGCCGGGCCTACGGGGATCTAGACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTAGAGCAGGCTGAc                                                         >  8:190949/1‑147 (MQ=255)

                                                                                               acTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGcc                                                   <  5:207755/149‑1 (MQ=255)

                                                                                                 tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCgg                                                 >  3:104341/1‑149 (MQ=255)

                                                                                                 tAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTCTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCAATTTCTATCTTAGAGCAGGCTGACGAGGCCgg                                                 >  7:473670/1‑149 (MQ=255)

                                                                                                     ttCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTc                                             >  4:2818/1‑149 (MQ=255)

                                                                                                       cGAAAGAATGCTTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGCGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCCCTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCg                                            <  5:366350/148‑1 (MQ=255)

                                                                                                        gaaagaaaGCGTACAGGTAAGTAACAATGAAAAAAATTTCTATTCTGGATGCACGGCCTACGGGGATCTACACCTTAATCAAGCATCTACAGCAACAAACTCCACTTTAAATTACTATCTTCGAGAAGGATGACGAGGCCGGTCTCgg                                           >  6:400707/1‑148 (MQ=255)

                                                                                                        gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAATTTTCTATTTTTGTTTCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCtt                                                                      <  4:322948/121‑1 (MQ=255)

                                                                                                        gAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCtt                                                                      >  3:322948/1‑121 (MQ=255)

                                                                                                         aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGa                                         >  1:321107/1‑149 (MQ=255)

                                                                                                         aaaGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGa                                         >  7:354831/1‑149 (MQ=255)

                                                                                                             aaTGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGCGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGc                                      >  2:335818/1‑148 (MQ=255)

                                                                                                               tGCGTACAGGTAAGTAACAATGAAAAATATTTTTTTTTTTGTTTCGGGGACTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACtttctatttct                                                                           <  4:383876/109‑1 (MQ=255)

                                                                                                                      aGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtga                            >  4:115924/1‑149 (MQ=255)

                                                                                                                         taagtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtgatga                         <  3:42609/149‑1 (MQ=255)

                                                                                                                           agtaaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAg                        <  7:269192/148‑1 (MQ=255)

                                                                                                                             taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtg                             >  2:52350/1‑141 (MQ=255)

                                                                                                                             taaCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGtg                             <  1:52350/141‑1 (MQ=255)

                                                                                                                               aCAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGaaaa                   <  5:225679/149‑1 (MQ=255)

                                                                                                                                cAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAAc                  <  1:335818/149‑1 (MQ=255)

                                                                                                                                 aaTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                     <  5:99871/129‑1 (MQ=255)

                                                                                                                                 aaTGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGcc                                     >  6:99871/1‑129 (MQ=255)

                                                                                                                                     aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGgtgt                                              <  3:345824/116‑1 (MQ=255)

                                                                                                                                     aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaa              <  3:241477/148‑1 (MQ=255)

                                                                                                                                     aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaa              >  7:405107/1‑148 (MQ=255)

                                                                                                                                     aaaaaaaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaa              >  1:6838/1‑148 (MQ=255)

                                                                                                                                       aaaaaTTGCTTTTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGAGGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCaaaaa           <  2:403569/149‑1 (MQ=255)

                                                                                                                                          aaTTTCTATTGTGGTTGCCGGGACTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                     <  6:287885/88‑1 (MQ=255)

                                                                                                                                          aaTTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTc                                                                     >  5:287885/1‑88 (MQ=255)

                                                                                                                                            ttGTTATTGTGGTTTCCGGGCCTACGGTGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAatgat       <  4:104341/148‑1 (MQ=255)

                                                                                                                                                tATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCTCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGATGACGAGCCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTc                >  1:282536/1‑135 (MQ=255)

                                                                                                                                                  ttGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTg   <  8:354831/146‑1 (MQ=255)

CCTGACAGAATTTAATCAAGGGCGGTTAGCGCCCTTTTCATCCCTGTCTGAAATTTCTCAAATTCTAAAAATCTCAACCAAACTTATCTGATAACACTAAATTCGAAAGAATGCGTACAGGTAAGTAACAATGAAAAAAATTGCTATTGTGGGTGCCGGGCCTACGGGGATCTACACCTTATTCTCGCTTCTACAGCAACAAACTCCACTTTCTATTTCTATCTTCGAGCAGGCTGACGAGGCCGGTGTCGGGATGCCATACAGTGATGAGGAAAACTCAAAAATGATGCTGG  >  NC_000913/1747001‑1747293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`