Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,896,377	G→A	5.2%	D70N (GAT→AAT)	nudL →	putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,896,377	0	G	A	5.2%	246.7 / 2.1	76	D70N (GAT→AAT)	nudL	putative CoA pyrophosphohydrolase, weak 3‑phosphohydroxypyruvate phosphatase
Reads supporting (aligned to +/- strand):  ref base G (34/38);  new base A (2/2);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTAT  >  NC_000913/1896230‑1896525                                                                                                                                                    |                                                                                                                                                     cAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGa                                                                                                                                                     <  6:13068/149‑1 (MQ=255)  aaaTTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGAt                                                                                                                                                    <  2:281308/149‑1 (MQ=255)    aTTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGa                                                                                                                                                  <  7:17854/149‑1 (MQ=255)         ccGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGaca                                                                                                                                                >  8:93551/1‑146 (MQ=255)         ccGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGaca                                                                                                                                                <  7:93551/146‑1 (MQ=255)                 cccTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCa                                                                                                                                     <  2:305060/149‑1 (MQ=255)                   cTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGc                                                                                                                                   <  3:248350/149‑1 (MQ=255)                       atcatcGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATc                                                                                                                               <  5:260341/149‑1 (MQ=255)                         catcGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGTAGTCGATGACACGGACGCATCAGCTATcgc                                                                                                                             >  6:435359/1‑149 (MQ=255)                                  gctgctGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCt                                                                                                                    >  8:186081/1‑149 (MQ=255)                                       tgtgTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGAt                                                                                                                                                    >  5:172640/1‑112 (MQ=255)                                       tgtgTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGAt                                                                                                                                                    <  6:172640/112‑1 (MQ=255)                                             aaTCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                                                                                                                                    <  7:232565/122‑1 (MQ=255)                                             aaTCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                                                                                                                                    >  8:232565/1‑122 (MQ=255)                                             aaTCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCt                                                                                                          <  4:311786/148‑1 (MQ=255)                                              aTCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTg                                                                                                         <  2:148633/148‑1 (MQ=255)                                               tCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaa                                                                                                       <  8:473839/149‑1 (MQ=255)                                                ccccATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTg                                                                                                         >  6:59085/1‑146 (MQ=255)                                                 cccATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaag                                                                                                      >  8:296020/1‑148 (MQ=255)                                                  ccATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCCGCGCGAAGCTgaag                                                                                                      >  6:274457/1‑147 (MQ=255)                                                    aTCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAagaaga                                                                                                  <  6:156479/149‑1 (MQ=255)                                                          cggcGACCGCAACCGGGGTTGTTGCTGACTCAGCGGTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGc                                                                                            <  5:230471/146‑1 (MQ=255)                                                           gtcGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGTAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCt                                                                                           <  5:435359/149‑1 (MQ=255)                                                           gtcGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGc                                                                                            >  4:184195/1‑148 (MQ=255)                                                                          gggtgttgCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCGGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTAGCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTccgcc                                                                            <  1:223231/146‑1 (MQ=255)                                                                               ttgCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTg                                                                        <  3:184195/148‑1 (MQ=255)                                                                                 gCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGaa                                                                      <  7:186081/148‑1 (MQ=255)                                                                                 gCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAg                                                                     >  4:168449/1‑149 (MQ=255)                                                                                      cTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaagaa                                                                                                    <  3:261342/113‑1 (MQ=255)                                                                                      cTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaagaa                                                                                                    >  4:261342/1‑113 (MQ=255)                                                                                       tCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                                                                                                                                    >  1:320343/1‑80 (MQ=255)                                                                                       tCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAg                                                                                                                                    <  2:320343/80‑1 (MQ=255)                                                                                       tCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCg                                                               >  1:50125/1‑149 (MQ=255)                                                                                         aGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGc                                                             >  6:308101/1‑149 (MQ=255)                                                                                         aGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGc                                                             >  4:332414/1‑149 (MQ=255)                                                                                            gTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGtg                                                          >  7:273780/1‑149 (MQ=255)                                                                                                 aTTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTgcc                                                     >  1:174952/1‑149 (MQ=255)                                                                                                  ttCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCCGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTgccg                                                    <  5:274457/149‑1 (MQ=255)                                                                                                    cATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTgccgc                                                   <  3:168449/148‑1 (MQ=255)                                                                                                        tGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGGCACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                              >  6:11405/1‑149 (MQ=255)                                                                                                          cGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTc                                              <  2:50125/147‑1 (MQ=255)                                                                                                            tAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATa                                          <  3:332414/149‑1 (MQ=255)                                                                                                            tAAACACGCTGGACAAGTGGCATACACTGGAGGTGCAGTCGATTACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACAGCCTTCCGACGTTGAATTTa                                                                  >  6:102918/1‑125 (MQ=255)                                                                                                            tAAACACGCTGGACAAGTGGCAGACCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTa                                                                  <  5:102918/125‑1 (MQ=255)                                                                                                             aaaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                 >  1:14768/1‑141 (MQ=255)                                                                                                             aaaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                 <  2:14768/141‑1 (MQ=255)                                                                                                              aaCACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGc                                        >  8:335160/1‑149 (MQ=255)                                                                                                                       gACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                <  7:335160/148‑1 (MQ=255)                                                                                                                       gACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTg                                 <  7:296020/147‑1 (MQ=255)                                                                                                                       gACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGc                               >  5:125312/1‑149 (MQ=255)                                                                                                                          aaGTGGCATTCCCTGGAGGTGCAGTCAATGACACGGCCGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGcagc                            >  1:426034/1‑146 (MQ=255)                                                                                                                          aaGTGGCATTCCCTGGAGGTGCAGTCAATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                <  2:426034/145‑1 (MQ=255)                                                                                                                          aaGTGGCATTCCCTGGAGGTGCAGTCAATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                >  1:426037/1‑145 (MQ=255)                                                                                                                          aaGTGGCATTCCCTGGAGGTGCAGTCAATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTgg                                <  2:426037/145‑1 (MQ=255)                                                                                                                            gTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTAc                            >  3:176921/1‑147 (MQ=255)                                                                                                                                 aTTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCa                          <  4:105284/144‑1 (MQ=255)                                                                                                                                 aTTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCa                          >  3:105284/1‑144 (MQ=255)                                                                                                                                  ttCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAc                    >  1:78480/1‑149 (MQ=255)                                                                                                                                    cccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAcc                   <  5:308101/148‑1 (MQ=255)                                                                                                                                     ccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAcccc                 <  5:59085/149‑1 (MQ=255)                                                                                                                                     ccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAccc                  >  4:279052/1‑148 (MQ=255)                                                                                                                                     ccTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAccc                  >  3:73155/1‑148 (MQ=255)                                                                                                                                      cTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                 >  8:233986/1‑116 (MQ=255)                                                                                                                                      cTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGccc                                                 <  7:233986/116‑1 (MQ=255)                                                                                                                                      cTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAAccc                  >  8:463010/1‑147 (MQ=255)                                                                                                                                         gAGGTGCAGTCGATGGCACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAg               <  5:11405/147‑1 (MQ=255)                                                                                                                                         gAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAg               <  4:73155/147‑1 (MQ=255)                                                                                                                                         gAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTg             >  7:263915/1‑149 (MQ=255)                                                                                                                                         gAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTg             >  2:65595/1‑149 (MQ=255)                                                                                                                                            gTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTc          <  7:463010/149‑1 (MQ=255)                                                                                                                                             tGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCg         <  6:125312/149‑1 (MQ=255)                                                                                                                                              gCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaagaa                                                                                                    >  1:444856/1‑57 (MQ=255)                                                                                                                                              gCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTgaagaa                                                                                                    <  2:444856/57‑1 (MQ=255)                                                                                                                                               cAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCgg        >  2:302035/1‑148 (MQ=255)                                                                                                                                               cAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGc       <  2:78480/149‑1 (MQ=255)                                                                                                                                                aGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGAAAGCGTCACTGGCTACCAGGTAACCCCAGTGCTCCGc       >  4:337948/1‑148 (MQ=255)                                                                                                                                                    gATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTAt  >  7:374979/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CAAATTAACCGGGAAACCCTAAATCATCGTCAGGCTGCTGTGTTAATCCCCATCGTCCGTCGACCGCAACCGGGGTTGTTGCTGACTCAGCGTTCGATTCATCTGCGTAAACACGCTGGACAAGTGGCATTCCCTGGAGGTGCAGTCGATGACACGGACGCATCAGCTATCGCCGCCGCGCTGCGCGAAGCTGAAGAAGAGGTCGCTATACCGCCTTCCGCCGTTGAAGTTATCGGCGTGCTGCCGCCCGTCGATAGCGTCACTGGCTACCAGGTAACCCCAGTGGTCGGCATTAT  >  NC_000913/1896230‑1896525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑