Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,076,298 T→A 11.1% V507E (GTA→GAA)  yeeR → CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,076,2980TA11.1% 244.0 / 20.2 90V507E (GTA→GAA) yeeRCP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (42/38);  new base A (6/4);  total (48/42)
Fisher's exact test for biased strand distribution p-value = 7.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

CAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGC  >  NC_000913/2076151‑2076443
                                                                                                                                                   |                                                                                                                                                 
cAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTa                                                                                                                                                  <  8:299706/149‑1 (MQ=255)
 aGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTag                                                                                                                                                 <  1:138499/149‑1 (MQ=255)
    ccGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAgagg                                                                                                                                              <  1:78730/149‑1 (MQ=255)
     cGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAgagga                                                                                                                                             <  3:383385/149‑1 (MQ=255)
            tCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGa                                                                                                                                      >  8:200657/1‑149 (MQ=255)
              ggTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGAc                                                                                                                                     <  6:186814/148‑1 (MQ=255)
              ggTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACg                                                                                                                                    >  8:110746/1‑149 (MQ=255)
               gTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGc                                                                                                                                   >  7:470515/1‑149 (MQ=255)
                   ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTa                                                                                                                                                  <  7:150419/130‑1 (MQ=255)
                   ggCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTa                                                                                                                                                  >  8:150419/1‑130 (MQ=255)
                    gCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGc                                                                                                                              <  7:110746/149‑1 (MQ=255)
                    gCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGc                                                                                                                              >  3:191069/1‑149 (MQ=255)
                         cGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAgc                                                                                                                          <  3:66663/148‑1 (MQ=255)
                           aCGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCa                                                                                                                       <  8:142627/149‑1 (MQ=255)
                             ggACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTagag                                                                                                                                               >  3:149525/1‑123 (MQ=255)
                              gACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGcc                                                                                                                    <  4:191069/149‑1 (MQ=255)
                               aCAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGa                                                                                                                            <  6:19845/140‑1 (MQ=255)
                               aCAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGa                                                                                                                            >  5:19845/1‑140 (MQ=255)
                                   aaCGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCg                                                                                                                                >  4:307584/1‑132 (MQ=255)
                                   aaCGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCg                                                                                                                                <  3:307584/132‑1 (MQ=255)
                                   aaCGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCAtt                                                                                                               <  1:453619/149‑1 (MQ=255)
                                      ggACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCaa                                                                                                            >  2:147745/1‑149 (MQ=255)
                                         cAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACgg                                                                                                         <  7:200657/149‑1 (MQ=255)
                                            tATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGc                                                                                                                              >  2:206675/1‑125 (MQ=255)
                                            tATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGc                                                                                                                              <  1:206675/125‑1 (MQ=255)
                                            tATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCAt                                                                                                                >  2:336187/1‑139 (MQ=255)
                                            tATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCAt                                                                                                                <  1:336187/139‑1 (MQ=255)
                                            tATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGcc                                                                                                      >  2:320607/1‑149 (MQ=255)
                                                gTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTg                                                                                                   >  3:108564/1‑148 (MQ=255)
                                                   aCATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGaaaa                                                                                               >  6:1317/1‑149 (MQ=255)
                                                    cATGGGAAATAATGCCCCCAAGCAACTGGAAGACCCCCGAGATCAGTATGCCGGCCCTGTTGAAACCATGAGCAATAAGATCAGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCAATCACCCGGCACTGAacac                                                                                              >  7:226792/1‑149 (MQ=255)
                                                     aTGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAAcc                                                                                             <  1:147745/149‑1 (MQ=255)
                                                     aTGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAAcc                                                                                             <  7:199864/149‑1 (MQ=255)
                                                       gggAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGcc                                                                                                      >  5:377053/1‑138 (MQ=255)
                                                       gggAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGcc                                                                                                      <  6:377053/138‑1 (MQ=255)
                                                                   ccccATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTg                                                                                          <  3:144231/138‑1 (MQ=255)
                                                                   ccccATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTg                                                                                          >  4:144231/1‑138 (MQ=255)
                                                                   ccccATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCt                                                                               >  4:93634/1‑149 (MQ=255)
                                                                    cccATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGAGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCtt                                                                              >  1:73702/1‑149 (MQ=255)
                                                                     ccATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCtt                                                                              >  8:435088/1‑148 (MQ=255)
                                                                       aTGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCaa                                                                                                            <  8:163496/116‑1 (MQ=255)
                                                                       aTGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCaa                                                                                                            >  7:163496/1‑116 (MQ=255)
                                                                       aTGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCCACGGGCCCTGAAAACCCTGGACCGCCATCTTCAt                                                                           >  3:252650/1‑149 (MQ=255)
                                                                         gCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGCTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGa                                                                         >  2:362334/1‑149 (MQ=255)
                                                                         gCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTCGACCGCCATCTTCATGa                                                                         >  8:85799/1‑149 (MQ=255)
                                                                              tGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCg                                                                    >  2:317273/1‑149 (MQ=255)
                                                                              tGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGAAGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCg                                                                    >  4:231837/1‑149 (MQ=255)
                                                                              tGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAAACATGACGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATCACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCg                                                                    >  6:390177/1‑149 (MQ=255)
                                                                                gAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGc                                                                  >  8:196535/1‑149 (MQ=255)
                                                                                gAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGc                                                                  >  3:203978/1‑149 (MQ=255)
                                                                                gAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACCGGCCCTGAAAACCCTGGACCGCCATCTTCATGACCCCGGc                                                                  >  4:241675/1‑149 (MQ=255)
                                                                                   gTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCa                                                                            <  5:65529/136‑1 (MQ=255)
                                                                                   gTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCa                                                                            >  6:65529/1‑136 (MQ=255)
                                                                                      ccccGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTg                                                                                          <  7:446606/119‑1 (MQ=255)
                                                                                      ccccGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTg                                                                                          >  8:446606/1‑119 (MQ=255)
                                                                                       cccGTGATCAGTATGCCGGCGCTGTTGAAACAATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATTACGCCCGGCGAGCGCAGCCTCATTCAACGCGCCCTGAAAACCCAGGACCCCCATCTTCAAGAACCCcgcgagccc                                                            >  2:439461/1‑143 (MQ=255)
                                                                                        ccGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCtt                                                          >  7:3431/1‑149 (MQ=255)
                                                                                         cGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTc                                                         >  2:403885/1‑149 (MQ=255)
                                                                                           tGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCAc                                                       <  1:317273/149‑1 (MQ=255)
                                                                                              tCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGc                                                                  <  8:317219/135‑1 (MQ=255)
                                                                                              tCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGc                                                                  >  7:317219/1‑135 (MQ=255)
                                                                                                aGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCa                                                  <  7:435088/149‑1 (MQ=255)
                                                                                                aGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGACGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCa                                                  <  3:231837/149‑1 (MQ=255)
                                                                                                 gTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTgg                                                              >  8:107157/1‑136 (MQ=255)
                                                                                                 gTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTgg                                                              <  7:107157/136‑1 (MQ=255)
                                                                                                      ccGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTg                                            >  7:466757/1‑149 (MQ=255)
                                                                                                       cGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGCTGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTgc                                           >  2:165858/1‑149 (MQ=255)
                                                                                                       cGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAAc                                                                                              >  4:380046/1‑98 (MQ=255)
                                                                                                       cGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAAc                                                                                              <  3:380046/98‑1 (MQ=255)
                                                                                                         gcgcTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTgcag                                         >  5:202781/1‑149 (MQ=255)
                                                                                                          cgcTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGCGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTgcagc                                        <  5:175526/149‑1 (MQ=255)
                                                                                                          cgcTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTgcagc                                        <  3:93634/149‑1 (MQ=255)
                                                                                                               ttGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACgg                                    <  4:108564/148‑1 (MQ=255)
                                                                                                               ttGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACgg                                    <  8:466757/148‑1 (MQ=255)
                                                                                                                 gAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGaa                                 >  7:377871/1‑149 (MQ=255)
                                                                                                                  aaaCCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGaa                                 <  1:165858/148‑1 (MQ=255)
                                                                                                                  aaaCCATGAGGAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCTGCACgggtg                                 <  8:74562/148‑3 (MQ=255)
                                                                                                                     ccATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                             <  1:403885/149‑1 (MQ=255)
                                                                                                                        tGACGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCCGCGAGCGCAGCCTCATTCAACTGGCCCTCAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTCAAGCACGGGAATGCCtca                          >  4:216305/1‑147 (MQ=255)
                                                                                                                           ggAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTc                       <  8:3431/149‑1 (MQ=255)
                                                                                                                            gAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                             >  3:131900/1‑142 (MQ=255)
                                                                                                                            gAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGc                             <  4:131900/142‑1 (MQ=255)
                                                                                                                            gAATAAGATCCGCGAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCt                      >  4:84373/1‑149 (MQ=255)
                                                                                                                               tAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGaa                   <  7:196535/149‑1 (MQ=255)
                                                                                                                               tAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGaa                   <  6:202781/149‑1 (MQ=255)
                                                                                                                                 aGATCCGCGATGGGAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACa                 >  2:366663/1‑149 (MQ=255)
                                                                                                                                  gATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACAt                >  1:64070/1‑149 (MQ=255)
                                                                                                                                       gcgAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgg           <  1:320607/149‑1 (MQ=255)
                                                                                                                                        cgAAGGTAAAGAAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCgg           >  5:273513/1‑148 (MQ=255)
                                                                                                                                                aagaagAGGAGAGATGACGGCCTGCGAGCGCTGCCTCATTCAACGGGCCCTGAAAACCCTGGACGTCCATCTTCATGAAACCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAg   <  5:390177/148‑1 (MQ=255)
                                                                                                                                                aaGTAGAGGAGAGATTCCGCCCGGCGAGAGCAGCCTCAATCCACGGGCCCTGAACACCCTGGACCACCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACTGGAATGGCTGATTCTGAACATGGCGGGACTGGAGc  <  8:470515/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CAGCCCGTAGCGTCGGTGCGGCATTCGACGGACAAAACGGACAGTATCGTTACATGGGAAATAATGGCCCCATGCAACTGGAAGTCCCCCGTGATCAGTATGCCGGCGCTGTTGAAACCATGAGGAATAAGATCCGCGAAGGTAAAGTAGAGGAGAGATGACGCCCGGCGAGCGCAGCCTCATTCAACGGGCCCTGAAAACCCTGGACCGCCATCTTCATGAACCCGGCGTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGC  >  NC_000913/2076151‑2076443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: