Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,142,505 G→C 6.2% R148R (CGC→CGG udk ← uridine/cytidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,142,5050GC 6.2% 431.4 / 16.0 129R148R (CGC→CGGudkuridine/cytidine kinase
Reads supporting (aligned to +/- strand):  ref base G (61/60);  new base C (3/5);  total (64/65)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

Only 100 of 136 total aligned reads displayed.

GGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTT  >  NC_000913/2142357‑2142653
                                                                                                                                                    |                                                                                                                                                    
ggTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcgg                                                                                                                                                      <  4:189968/149‑1 (MQ=255)
 gTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTAAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATgcggc                                                                                                                                                     <  1:343988/149‑1 (MQ=255)
          gccgcGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGCCGCATCAAGc                                                                                                                                            <  6:381490/149‑1 (MQ=255)
                 ggCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATc                                                                                                                                     <  4:223843/149‑1 (MQ=255)
                    aCGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAg                                                                                                                                  >  3:299947/1‑149 (MQ=255)
                      gATAATGTCCGCATATTTTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGTTTTTTTGATATTGCGCCATCACTGAATCAATTGAACGCCCACGCTCGTTAACGTAACGCTTGATGCGGCGCATCCGGCAGATATCAAGCg                                                                                                                                >  5:265230/1‑149 (MQ=255)
                      gATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCg                                                                                                                                <  2:73217/149‑1 (MQ=255)
                        tAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCggg                                                                                                                              <  5:448077/149‑1 (MQ=255)
                        tAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCggg                                                                                                                              <  1:199013/149‑1 (MQ=255)
                          aTGTCCGCATATTGTTTAGAAGGCTCAATCAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGGTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCggg                                                                                                                              <  6:19644/147‑1 (MQ=255)
                           tGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCCTGATGCGCCGCATCAAGcgtgac                                                                                                                                       >  6:63005/1‑132 (MQ=255)
                             tCCGCATATTGTTTAGAATGCTCAATGAATTGCAGGAAAATCGGGCGCACGGTTTTTTTATATTGCGCAATCACTGAATCCATTGAACGCCCACGCTCGTAAAAGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATc                                                                                                                         <  5:83439/149‑1 (MQ=255)
                                gCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAAc                                                                                                                      >  4:392607/1‑149 (MQ=255)
                                gCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAAc                                                                                                                      >  1:19143/1‑149 (MQ=255)
                                       gTTTAGAAGGCTCAATGAATTGCAGGAACATCGGTCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAAt                                                                                                                <  6:2818/148‑1 (MQ=255)
                                        tttAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATgg                                                                                                              <  8:21851/149‑1 (MQ=255)
                                          tAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATg                                                                                                               >  8:14481/1‑146 (MQ=255)
                                           aGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAAt                                                                                                                <  8:437510/144‑1 (MQ=255)
                                            gAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCa                                                                                                                                   >  7:299648/1‑124 (MQ=255)
                                            gAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCa                                                                                                                                   <  8:299648/124‑1 (MQ=255)
                                            gAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaa                                                                                                          <  7:371015/149‑1 (MQ=255)
                                             aaGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCggg                                                                                                                              >  5:267920/1‑128 (MQ=255)
                                                  tCAATGAATTGTAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATg                                                                                                               >  3:191627/1‑138 (MQ=255)
                                                  tCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaag                                                                                                    >  1:158263/1‑149 (MQ=255)
                                                  tCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaag                                                                                                    >  7:107468/1‑149 (MQ=255)
                                                  tCAATGAATTGCAGGAACATCGGGCGCACCGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTACCCTCACGCTTGATGCGGCGCATCCGGCAGATATCCAGCGGCCTATCAACGAAAATGGAGaactgaac                                                                                                    >  5:112884/1‑143 (MQ=255)
                                                    aaTGAATTGCAGGAACATCGGGCGCACGGTTTTTTTATATTGAGCCATCACTTAATCCATTGAAAGCCAACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGTCAGATATACAGCTGGGTATCAACGAAAATGGATaagttaagtt                                                                                                  >  3:54158/1‑149 (MQ=255)
                                                    aaTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaag                                                                                                    <  2:158263/147‑1 (MQ=255)
                                                    aaTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaagttaag                                                                                                    <  1:183976/147‑1 (MQ=255)
                                                     aTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTc                                                                                                 >  7:239908/1‑149 (MQ=255)
                                                     aTGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTc                                                                                                 <  7:50152/149‑1 (MQ=255)
                                                      tGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGGATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCg                                                                                                >  2:345488/1‑149 (MQ=255)
                                                      tGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCg                                                                                                                                >  3:81297/1‑117 (MQ=255)
                                                      tGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCg                                                                                                                                <  4:81297/117‑1 (MQ=255)
                                                        aaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGcgt                                                                                                                                          <  7:254179/105‑3 (MQ=255)
                                                        aaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGcgt                                                                                                                                          >  8:254179/1‑103 (MQ=255)
                                                        aaTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTc                                                                                              <  2:279621/149‑1 (MQ=255)
                                                         aTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCa                                                                                             <  5:175810/149‑1 (MQ=255)
                                                         aTTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCCTGATGCGCCGCATCAAGcgtgac                                                                                                                                       >  4:329426/1‑102 (MQ=255)
                                                          ttGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCAc                                                                                            <  6:381612/149‑1 (MQ=255)
                                                              aGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGCCGCATc                                                                                                                                                <  2:373136/93‑1 (MQ=255)
                                                                gAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaaa                                                                                       <  8:140778/148‑1 (MQ=255)
                                                                gAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCaaa                                                                                       >  7:140778/1‑148 (MQ=255)
                                                                gAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCAGCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAc                                                                                      >  7:447556/1‑149 (MQ=255)
                                                                    aTCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAgg                                                                                                                                             <  6:32199/90‑1 (MQ=255)
                                                                    aTCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcgcg                                                                                   >  1:44938/1‑148 (MQ=255)
                                                                    aTCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcgcg                                                                                   >  4:189887/1‑148 (MQ=255)
                                                                        ggCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATcc                                                                              <  6:199805/149‑1 (MQ=255)
                                                                            cACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGt                                                                            >  6:392479/1‑147 (MQ=255)
                                                                            cACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCa                                                                          <  7:14481/149‑1 (MQ=255)
                                                                             aCGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAg                                                                         <  4:240958/149‑1 (MQ=255)
                                                                             aCGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAg                                                                         >  8:324399/1‑149 (MQ=255)
                                                                               ggTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATc                                                                                                                                     >  4:218512/1‑87 (MQ=255)
                                                                               ggTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCTGGGTATCAACGAAAATGGAGAAGATAAGTTCGACACGCAAACGCGCATCCGTCAGc                                                                        >  5:378129/1‑148 (MQ=255)
                                                                                   ttttGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAATTGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCAGTCAGcaaca                                                                    >  8:288031/1‑148 (MQ=255)
                                                                                       gATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAAt                                                                <  8:6632/148‑1 (MQ=255)
                                                                                          atTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCa                                                                                                                                   >  3:85306/1‑78 (MQ=255)
                                                                                           ttGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCtt                                                           <  3:271984/149‑1 (MQ=255)
                                                                                             gcgcCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAAt                                                                >  4:176271/1‑142 (MQ=255)
                                                                                             gcgcCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTc                                                          <  7:324399/148‑1 (MQ=255)
                                                                                              cgcCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCga                                                        <  2:44938/149‑1 (MQ=255)
                                                                                                 cATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGaa                                                     >  5:448904/1‑149 (MQ=255)
                                                                                                 cATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGaa                                                     >  5:317095/1‑149 (MQ=255)
                                                                                                    cACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatga                                                  >  2:380587/1‑149 (MQ=255)
                                                                                                     aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAAc                                                                                                                      >  7:447943/1‑80 (MQ=255)
                                                                                                     aCTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAAc                                                                                                                      <  8:447943/80‑1 (MQ=255)
                                                                                                      cTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAatgat                                                 >  6:325349/1‑148 (MQ=255)
                                                                                                       tGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCa                                                                                                                                           <  8:377328/57‑1 (MQ=255)
                                                                                                       tGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCa                                                                                                                                           >  7:377328/1‑57 (MQ=255)
                                                                                                       tGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTGAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAAtgatga                                               >  2:115814/1‑149 (MQ=255)
                                                                                                         aaTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGAcc                                             >  7:45743/1‑149 (MQ=255)
                                                                                                         aaTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGAcc                                             >  5:361946/1‑149 (MQ=255)
                                                                                                          aTCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGAcc                                             <  3:189887/148‑1 (MQ=255)
                                                                                                             cATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGaa                                                                                                          >  1:144836/1‑84 (MQ=255)
                                                                                                              aTTGAACGCCCACTCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAAt                                                                >  6:297003/1‑125 (MQ=255)
                                                                                                              aTTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACcttctt                                        >  3:118802/1‑149 (MQ=255)
                                                                                                                  aaCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAg                                                                         <  8:55058/112‑1 (MQ=255)
                                                                                                                  aaCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAg                                                                         >  7:55058/1‑112 (MQ=255)
                                                                                                                  aaCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATAAGGCAGATATCCAGCGGGGTATCAACTAAAAGGGAGAAGTTAAGTTCGTCACGCAAACGCGCATACGTCAGCAACAAAATGCCGTCGAGAATGATGACCTTCTTCGGc                                    >  1:337986/1‑149 (MQ=255)
                                                                                                                   aCGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCt                                   <  6:448904/149‑1 (MQ=255)
                                                                                                                      cccACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCaa                                <  4:299947/149‑1 (MQ=255)
                                                                                                                      cccACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCaa                                <  8:107468/149‑1 (MQ=255)
                                                                                                                       ccACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCATTCGAGAATGATGACCTTCTTCGGCTCAAc                               >  6:458710/1‑149 (MQ=255)
                                                                                                                           gCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTTAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGt                            >  8:187304/1‑148 (MQ=255)
                                                                                                                               gTTAACGTCTCTCTTGATGCGGCGCATCAGTCAGATATCCAGCTGGGTATCATCGAAAATGGAGAAGTTAAGTTCGTCACGCATACGCGCATCCGTCAGCTACAAATTGCCTTCGATAATGATGACGTTCTTCGGCTCAACGGTCAcc                        <  2:337986/148‑1 (MQ=255)
                                                                                                                               gTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCg                       >  5:458115/1‑149 (MQ=255)
                                                                                                                                ttAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcgc                                                                                    >  2:6555/1‑87 (MQ=255)
                                                                                                                                ttAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAAcgc                                                                                    <  1:6555/87‑1 (MQ=255)
                                                                                                                                  aaCGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGttt                    >  5:273726/1‑149 (MQ=255)
                                                                                                                                   aCGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGATAAGGTTAGTTCGTCACGCAACAGCGCATCCGTCAGGAACAAAATGTCTTCGATAATGATGACCTTCTTCTGGTCAACCGTCACCGttt                    <  4:54158/148‑1 (MQ=255)
                                                                                                                                   aCGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGCGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttc                   <  6:273726/149‑1 (MQ=255)
                                                                                                                                       tctgcctGATGCGCCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGtttcttt                >  7:287614/7‑148 (MQ=255)
                                                                                                                                        aCGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa              >  5:180036/1‑149 (MQ=255)
                                                                                                                                        aCGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCa              <  5:325349/149‑1 (MQ=255)
                                                                                                                                           cTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAc           <  1:345488/149‑1 (MQ=255)
                                                                                                                                            ttGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcg          >  7:289726/1‑149 (MQ=255)
                                                                                                                                              gATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATAcgcg        >  2:164155/1‑149 (MQ=255)
                                                                                                                                                  cggcgCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTAt     <  5:392479/148‑1 (MQ=255)
                                                                                                                                                  cggcgCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTAt     <  3:260222/148‑1 (MQ=255)
                                                                                                                                                    gcgcATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGtt  >  1:103384/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGTTTTTCCCGCCGCGCGGCACGATAATGTCCGCATATTGTTTAGAAGGCTCAATGAATTGCAGGAACATCGGGCGCACGGTTTTTTGATATTGCGCCATCACTGAATCCATTGAACGCCCACGCTCGTTAACGTCACGCTTGATGCGGCGCATCAGGCAGATATCCAGCGGGGTATCAACGAAAATGGAGAAGTTAAGTTCGTCACGCAAACGCGCATCCGTCAGCAACAAAATGCCTTCGAGAATGATGACCTTCTTCGGCTCAACCGTCACCGTTTCTTTCATACGCGTATGTT  >  NC_000913/2142357‑2142653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: