Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,364,998 C→A 6.8% intergenic (+19/+20) nudI → / ← ais nucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,364,9980CA 6.8% 344.8 / 9.1 102intergenic (+19/+20)nudI/aisnucleoside triphosphatase/putative LPS core heptose(II)‑phosphate phosphatase
Reads supporting (aligned to +/- strand):  ref base C (46/48);  new base A (4/3);  total (50/52)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 102 total aligned reads displayed.

CTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAA  >  NC_000913/2364852‑2365141
                                                                                                                                                  |                                                                                                                                               
cTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACt                                                                                                                                                <  1:355069/148‑1 (MQ=255)
  gCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGc                                                                                                                                             >  1:456417/1‑149 (MQ=255)
     aaCCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTgg                                                                                                                                              <  5:389594/145‑1 (MQ=255)
     aaCCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTgg                                                                                                                                              >  6:389594/1‑145 (MQ=255)
     aaCCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGt                                                                                                                                          >  7:198251/1‑149 (MQ=255)
        cGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCa                                                                                                                                        >  2:453749/1‑148 (MQ=255)
          agaAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGAt                                                                                                                                     <  6:215848/149‑1 (MQ=255)
          agaAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCatca                                                                                                                                     <  8:198549/149‑4 (MQ=255)
            aaGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCatcatt                                                                                                                                   <  1:453749/149‑6 (MQ=255)
               tGAACATAATCGCAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGa                                                                                                                                  <  6:335525/147‑1 (MQ=255)
               tGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaaa                                                                                                                                <  3:195081/149‑1 (MQ=255)
               tGAAAATAAACGAAGAGTTTCAGGACGACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACTTTACGTTTGAAAGGTCTGCTGTAACAATTATGACAGCCATTACTGGCTGTCAGATTGa                                                                                                                                  >  5:335525/1‑147 (MQ=255)
                gAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaaaa                                                                                                                               >  6:240959/1‑149 (MQ=255)
                    ctAAACGAAGCGTTTCAGGACTCCGGGTGGGTAAAACCTGAAGCTCTGGTGCATTATGATTTGAATGTCGCCCCGCGAAAAACGTTAGGGTTGAAAGGTCGTCTGTAACAATGATGCCAGCCTTTACTGGCTGTCAGATTGAAAATTTa                                                                                                                           <  1:144113/148‑1 (MQ=255)
                     tAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAg                                                                                                                          <  8:341477/149‑1 (MQ=255)
                       accGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGGGCATTATGATTTGAATGGCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTg                                                                                                                        <  2:59477/147‑1 (MQ=255)
                       aaCGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGt                                                                                                                         <  1:212342/148‑1 (MQ=255)
                       aaCGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGt                                                                                                                         <  7:401740/148‑1 (MQ=255)
                          gAAGAGTTTCAGGACTACGCGTGGGTAAACCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGtt                                                                                                                     <  5:243638/149‑1 (MQ=255)
                          gAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGt                                                                                                                      >  7:330643/1‑148 (MQ=255)
                            agagTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTaa                                                                                                                   <  7:275946/149‑1 (MQ=255)
                               gTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGa                                                                                                                <  6:216045/149‑1 (MQ=255)
                                  tCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCt                                                                                                                                            <  3:344062/118‑1 (MQ=255)
                                  tCAGGACTACGCGTGGCTAAAACCTGAAGATCTTGTGCATTATGATTTGAATGTCGCAACCCGAAAAACGTTACGTTTGAAATGTCTTCTGTAACAAAGATGACAGCCATAACTGGCt                                                                                                                                            >  4:344062/1‑118 (MQ=255)
                                    aGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcc                                                                                                           <  7:123156/149‑1 (MQ=255)
                                    aGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCa                                                                                                                                        >  6:135008/1‑120 (MQ=255)
                                    aGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCAGTAATGGCTGTCa                                                                                                                                        <  5:135008/120‑1 (MQ=255)
                                      gACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTccc                                                                                                          >  4:347930/1‑148 (MQ=255)
                                         tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAATAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcc                                                                                                           >  2:66877/1‑144 (MQ=255)
                                         tACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAATAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcc                                                                                                           <  1:66877/144‑1 (MQ=255)
                                              gTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCCTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGa                                                                                                  >  7:238098/1‑148 (MQ=255)
                                               tGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGAt                                                                                                 >  8:250526/1‑148 (MQ=255)
                                                  gTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAAc                                                                                             >  4:331206/1‑149 (MQ=255)
                                                    aaaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaa                                                                                                                                 >  5:38967/1‑111 (MQ=255)
                                                    aaaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGaa                                                                                                                                 <  6:38967/111‑1 (MQ=255)
                                                     aaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTg                                                                                                                        <  2:424295/119‑1 (MQ=255)
                                                     aaaCCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTg                                                                                                                        >  1:424295/1‑119 (MQ=255)
                                                         cTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTTAAAATTTAGTGGt                                                                                                                      >  1:158184/1‑117 (MQ=255)
                                                           gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACttt                                                                                          >  5:87194/1‑143 (MQ=255)
                                                           gAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACttt                                                                                          <  6:87194/143‑1 (MQ=255)
                                                            aaGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATAACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCtttt                                                                                   <  7:159652/149‑1 (MQ=255)
                                                             aGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCttttt                                                                                  >  3:13202/1‑149 (MQ=255)
                                                               aTCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTc                                                                                 >  5:339097/1‑148 (MQ=255)
                                                                    gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                               >  3:342662/1‑113 (MQ=255)
                                                                    gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGaa                                                                                                               <  4:342662/113‑1 (MQ=255)
                                                                    gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatg                                                                           >  3:332008/1‑149 (MQ=255)
                                                                    gTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcat                                                                            <  6:339097/148‑1 (MQ=255)
                                                                           ctGATTTGAATGTCGCCCCCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCCTGAc                                                                     <  2:356975/147‑1 (MQ=255)
                                                                             gATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTa                                                                   >  2:87703/1‑148 (MQ=255)
                                                                              aTTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTaaa                                                                 >  7:63288/1‑149 (MQ=255)
                                                                                   aaTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATc                                                            <  3:331206/149‑1 (MQ=255)
                                                                                   aaTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATc                                                            <  8:330643/149‑1 (MQ=255)
                                                                                    aTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTATACCATcc                                                           <  2:456417/149‑1 (MQ=255)
                                                                                    aTGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATc                                                            >  7:278795/1‑148 (MQ=255)
                                                                                             cccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAcc                                                               <  2:98737/136‑1 (MQ=255)
                                                                                             cccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAAcc                                                               >  1:98737/1‑136 (MQ=255)
                                                                                              ccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACttt                                                                                          <  7:271059/108‑1 (MQ=255)
                                                                                              ccGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACttt                                                                                          >  8:271059/1‑108 (MQ=255)
                                                                                                gAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTaa                                                                  <  5:435353/130‑1 (MQ=255)
                                                                                                gAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTaa                                                                  >  6:435353/1‑130 (MQ=255)
                                                                                                gAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCACCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGt                                               >  6:55827/1‑149 (MQ=255)
                                                                                                        ttacgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGt                                       >  3:319774/1‑149 (MQ=255)
                                                                                                         tacgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTc                                      >  2:163981/1‑149 (MQ=255)
                                                                                                           cgttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcgc                                    <  4:13202/149‑1 (MQ=255)
                                                                                                           cgttTGAAAGGTCCTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcg                                     <  8:238098/148‑1 (MQ=255)
                                                                                                            gttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcgcg                                   >  1:236219/1‑149 (MQ=255)
                                                                                                            gttTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTcgcg                                   >  3:7120/1‑149 (MQ=255)
                                                                                                                gAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCAc                               >  1:163780/1‑149 (MQ=255)
                                                                                                                   aGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCt                            >  8:205906/1‑149 (MQ=255)
                                                                                                                    ggTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCt                            >  1:180273/1‑148 (MQ=255)
                                                                                                                      tcttctGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTAt                         <  2:180273/149‑1 (MQ=255)
                                                                                                                           tGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCtttt                                                                                   >  5:233271/1‑86 (MQ=255)
                                                                                                                           tGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCtttt                                                                                   <  6:233271/86‑1 (MQ=255)
                                                                                                                            gTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAg                   >  1:370978/1‑149 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACtt                                                                                           >  7:152945/1‑76 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACtt                                                                                           <  8:152945/76‑1 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCttt                     <  4:332008/146‑1 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTa                    <  3:347930/147‑1 (MQ=255)
                                                                                                                             tAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAg                   >  7:306125/1‑148 (MQ=255)
                                                                                                                               aCAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCGTTTTTACAAATTCATGACTAAACCATCCAGATAATCAGGTTTAAATTTCGCGTCACGCTTAGCTTTAGCaa                >  1:120990/1‑149 (MQ=255)
                                                                                                                                ctatgatgACAGCCATTAGTTGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcccc                                                                                                         <  8:54476/57‑1 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTcccc                                                                                                         >  7:54476/1‑59 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat               <  7:205906/149‑1 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat               <  8:306125/149‑1 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat               <  2:370978/149‑1 (MQ=255)
                                                                                                                                cAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAat               <  1:220109/149‑1 (MQ=255)
                                                                                                                                 aaTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTATCGCATGCCCCATCCAGATAACCTTTTCATTTTTCACCATGCATGACTAAACCATCCAGCTAATCAGGTTTAACTGTCGCGTCACGCTGATCTTTCGCAtta              >  5:309533/1‑149 (MQ=255)
                                                                                                                                 aaTGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAata              <  2:236219/149‑1 (MQ=255)
                                                                                                                                   tgatgaCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAatata            >  2:46059/1‑149 (MQ=255)
                                                                                                                                     atgaCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAatatat           <  5:240959/148‑1 (MQ=255)
                                                                                                                                      tgaCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGt         >  7:411428/1‑149 (MQ=255)
                                                                                                                                      tgaCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGt         <  1:163981/149‑1 (MQ=255)
                                                                                                                                      tgaCAGCCAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGt         >  6:41450/1‑149 (MQ=255)
                                                                                                                                        aCAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTc        <  1:87703/148‑1 (MQ=255)
                                                                                                                                         cAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCa       <  8:278795/148‑1 (MQ=255)
                                                                                                                                          aGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgcat                                                                        >  4:273350/1‑82 (MQ=255)
                                                                                                                                          aGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCAcatgcat                                                                        <  3:273350/82‑1 (MQ=255)
                                                                                                                                          aGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAgg     <  1:46059/149‑1 (MQ=255)
                                                                                                                                            ccAGTAATGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGc    >  1:284623/1‑148 (MQ=255)
                                                                                                                                             cATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAAGTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTATACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCaa  >  2:378966/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
CTGCCAACCGAGAAGTGAAAATAAACGAAGAGTTTCAGGACTACGCGTGGGTAAAACCTGAAGATCTGGTGCATTATGATTTGAATGTCGCCACCCGAAAAACGTTACGTTTGAAAGGTCTTCTGTAACAATGATGACAGCCATTACTGGCTGTCAGATTGAAAATTTAGTGGTTAACGAATTCCCCATCCAGATAAACTTTGCCTTTTTCCACATGCATGACTAAACCATCCAGATAATCAGGTTTAAATGTCGCGTCACGCTTATCTTTAGCAATATATGTCAGGCAA  >  NC_000913/2364852‑2365141

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: