Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,908 C→G 7.7% intergenic (+17/+22) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9080CG 7.7% 421.7 / 17.2 126intergenic (+17/+22)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base C (57/59);  new base G (7/3);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAA  >  NC_000913/2525762‑2526055
                                                                                                                                                  |                                                                                                                                                   
cgcAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTc                                                                                                                                                   <  8:224858‑M2/149‑2 (MQ=255)
       gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTcccggcag                                                                                                                                            <  5:238564‑M2/149‑9 (MQ=255)
        cACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTcccggcag                                                                                                                                            <  3:86662‑M2/148‑9 (MQ=255)
         aCTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTcccggcagta                                                                                                                                          >  5:42517‑M2/1‑139 (MQ=255)
           tgtgGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                         >  1:176540/1‑148 (MQ=255)
             tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGaa                                                                                                                                      <  4:86662/149‑1 (MQ=255)
             tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCcccggcagtaat                                                                                                                                        >  6:158696‑M2/1‑135 (MQ=255)
             tggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATATTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGaa                                                                                                                                      >  2:380366/1‑149 (MQ=255)
                tggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAAtt                                                                                                                                    >  8:309762/1‑148 (MQ=255)
                tggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAAtt                                                                                                                                    >  7:12624/1‑148 (MQ=255)
                    aTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCActc                                                                                                                               <  8:802/149‑1 (MQ=255)
                    aTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCcccggcagtaatcaatcagaa                                                                                                                               >  1:198234‑M2/1‑128 (MQ=255)
                       cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                            <  4:341914/149‑1 (MQ=255)
                       cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCta                                                                                                                             >  6:185940/1‑148 (MQ=255)
                        aaCATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                           >  4:4107/1‑149 (MQ=255)
                         aCATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTatat                                                                                                                          >  8:21622/1‑149 (MQ=255)
                            tCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTg                                                                                                                       <  7:334949/149‑1 (MQ=255)
                              acagaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCGATCATATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGa                                                                                                                      <  2:118276/148‑1 (MQ=255)
                              acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                     <  5:439318/149‑1 (MQ=255)
                               cacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                    >  2:24382/1‑149 (MQ=255)
                               cacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                    >  6:437430/1‑149 (MQ=255)
                                acaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                    >  4:120451/1‑148 (MQ=255)
                                  aTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACaa                                                                                                                 >  7:385676/1‑149 (MQ=255)
                                  aTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACaa                                                                                                                 >  8:395475/1‑149 (MQ=255)
                                   ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAAc                                                                                                                <  8:12624/149‑1 (MQ=255)
                                       tacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGAt                                                                                                            <  7:309762/149‑1 (MQ=255)
                                          ggggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTcccggca                                                                                                                                             <  7:214738‑M2/113‑8 (MQ=255)
                                          ggggATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTcccggca                                                                                                                                             >  8:214738‑M2/1‑106 (MQ=255)
                                                tCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAAc                                                                                                                >  3:298616/1‑136 (MQ=255)
                                                   gTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                                <  6:238564/149‑1 (MQ=255)
                                                      tCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATCCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCa                                                                                              <  4:5768/148‑1 (MQ=255)
                                                          tatGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                         <  5:185940/149‑1 (MQ=255)
                                                           atGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATATGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                         >  6:257057/1‑148 (MQ=255)
                                                              cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                         >  8:389827/1‑145 (MQ=255)
                                                              cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                         <  7:389827/145‑1 (MQ=255)
                                                               ggATTACAAACTGGGCGAATATTTTTACCCAGATGGCATTATATTCTTCATAAAATTTAACTTCTTTTTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgc                                                                                     <  5:339330/148‑1 (MQ=255)
                                                               ggATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                         >  3:423911/1‑96 (MQ=255)
                                                                gATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCcccggcagtaatcaatcagaagttaaattttatgga                                                                                                                <  8:461211‑M2/120‑37 (MQ=255)
                                                                gATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGAATGATTACTGCCGGGAGCcccggcagtaatcaatcagaagttaaatttgatgga                                                                                                                >  7:461211‑M2/1‑84 (MQ=255)
                                                                gATGACAAACGGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTAGATCTGATACACCGGATCCCCTTCCCGCCCAGCCATTAgcg                                                                                    >  4:47278/1‑148 (MQ=255)
                                                                    aCAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                                <  1:24382/148‑1 (MQ=255)
                                                                     cAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc                                                                              >  8:14696/1‑149 (MQ=255)
                                                                      aaaCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa                                                                                       <  3:350432/139‑1 (MQ=255)
                                                                      aaaCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                             >  8:50511/1‑149 (MQ=255)
                                                                      aaaCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAAAGGATCCCCTTCCCGCCCAGCCATTa                                                                                       >  4:350432/1‑139 (MQ=255)
                                                                       aaCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc                                                                            <  2:327410/149‑1 (MQ=255)
                                                                        aCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCt                                                                           <  3:4107/149‑1 (MQ=255)
                                                                         cTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCCTTGCCCTg                                                                          <  8:385676/149‑1 (MQ=255)
                                                                              cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                         <  3:102548/129‑1 (MQ=255)
                                                                              cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                     <  1:380366/149‑1 (MQ=255)
                                                                              cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtctc                                                                     <  2:360773/149‑1 (MQ=255)
                                                                               gaagaaTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                           >  1:126579/1‑126 (MQ=255)
                                                                               gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                    >  4:149872/1‑149 (MQ=255)
                                                                               gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCg                                                                    >  7:227898/1‑149 (MQ=255)
                                                                                aaGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                   <  3:120451/149‑1 (MQ=255)
                                                                                   aTTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTc                                                                                                     <  6:154679/112‑1 (MQ=255)
                                                                                   aTTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                                <  3:67448/149‑1 (MQ=255)
                                                                                        tACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                         >  6:143739/1‑71 (MQ=255)
                                                                                        tACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTAt                                                                                                                                         <  5:143739/71‑1 (MQ=255)
                                                                                         aCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTc                                                           <  3:149872/148‑1 (MQ=255)
                                                                                             ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCcccggcagtaatcaatcagaagttaaatttgatggagtagattatcgcatcctggtaaaaatcttc                                                                                  <  8:445611‑M2/121‑67 (MQ=255)
                                                                                             ggATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCcccggcagtaatcaatcagaagttaaatttgatggagtagattatcgcatcctggtaaaaatcttc                                                                                  >  7:445611‑M2/1‑55 (MQ=255)
                                                                                                  cGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCt                                                 <  7:50511/149‑1 (MQ=255)
                                                                                                   taTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAAc                                                                                                                <  1:55629/84‑1 (MQ=255)
                                                                                                   gATAATCTACTCCAACAAATTTAACTTCTGAATGAATACTGCCCGGGCTCCCGGCAGTATGAATTCACACTAAATCTCATACAAc                                                                                                                >  2:55629/1‑85 (MQ=255)
                                                                                                    aTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt                                                >  6:329554/1‑148 (MQ=255)
                                                                                                       aTCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGGCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg                                             <  5:257057/148‑1 (MQ=255)
                                                                                                            cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                        <  7:21622/148‑1 (MQ=255)
                                                                                                            cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggcg                                        <  2:176540/148‑1 (MQ=255)
                                                                                                             tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                      >  5:218595/1‑149 (MQ=255)
                                                                                                             tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                      >  7:96190/1‑149 (MQ=255)
                                                                                                               cATGAAATTTAACTTATGAATGATTACTGTCGGGGCTCCAGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCTCATTTCCGTTGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                      <  6:386087/147‑1 (MQ=255)
                                                                                                               cATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                      <  5:329554/147‑1 (MQ=255)
                                                                                                                aTCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTgg                                    >  8:41637/1‑148 (MQ=255)
                                                                                                                   aaaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                   >  7:10601/1‑114 (MQ=255)
                                                                                                                   aaaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                   <  8:10601/114‑1 (MQ=255)
                                                                                                                       ttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                    <  6:114419/125‑1 (MQ=255)
                                                                                                                          aCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACg                          <  8:352854/148‑1 (MQ=255)
                                                                                                                            ttCTTATTTATTAGTGCCGGGGCTACCGGCTTTATTTATTCACTGTATATGTTATAGAACGGCTTCCCTTCACGGCGAGTCATTAGGGCATTGCCCTGTTCTCGTACCACGTCCCATAAAGCTTCAGCGGAGGTGGATTGTGAACGGtt                       <  4:106387/149‑1 (MQ=255)
                                                                                                                            ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                        <  6:156260/84‑1 (MQ=255)
                                                                                                                            ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                        >  5:156260/1‑84 (MQ=255)
                                                                                                                            ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                            >  6:132913/1‑144 (MQ=255)
                                                                                                                            ttCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                            <  5:132913/144‑1 (MQ=255)
                                                                                                                              cTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttc                      <  6:218595/148‑1 (MQ=255)
                                                                                                                               tgattgatTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                    >  8:388218/1‑149 (MQ=255)
                                                                                                                                gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                   >  6:290567/1‑149 (MQ=255)
                                                                                                                                gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                   >  6:198031/1‑149 (MQ=255)
                                                                                                                                 attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGt                                      <  7:392976/129‑1 (MQ=255)
                                                                                                                                 attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                    <  7:316780/147‑1 (MQ=255)
                                                                                                                                 attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc                  >  3:134248/1‑149 (MQ=255)
                                                                                                                                  ttgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                           >  7:454076/1‑75 (MQ=255)
                                                                                                                                      ttACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGa                                   >  4:275429/1‑127 (MQ=255)
                                                                                                                                       tACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc                           >  1:329099/1‑134 (MQ=255)
                                                                                                                                       tACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa            >  8:440549/1‑149 (MQ=255)
                                                                                                                                         cTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                            >  4:53414/1‑131 (MQ=255)
                                                                                                                                             cGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                    <  3:429735/103‑1 (MQ=255)
                                                                                                                                             cGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATaaa                                                    >  4:429735/1‑103 (MQ=255)
                                                                                                                                               gggCTcccggcagtaatcaatcagaagttaaatttgatggagtagattatcgcatcctggtaaaaatcttcgcccagtttgtcatccgcatatcgatactgaatccccgtagtaatgtgtgatgttgcattccaccacagtgcaactgc    >  2:198234‑M2/1‑5 (MQ=255)
                                                                                                                                               gggCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa      >  4:323769/1‑147 (MQ=255)
                                                                                                                                                 gCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCaa  >  3:99078/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAA  >  NC_000913/2525762‑2526055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: