Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,024,014 T→A 5.5% L900Q (CTG→CAG)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,024,0140TA 5.5% 226.6 / 3.5 73L900Q (CTG→CAG) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base T (34/35);  new base A (2/2);  total (36/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.66e-01

AAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCA  >  NC_000913/3023867‑3024148
                                                                                                                                                   |                                                                                                                                      
aaaTCTACGGTGCCACCCTGCGTTCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCTCGCTGTGCTg                                                                                                                                       <  1:286730/149‑1 (MQ=255)
aaaTCTAAGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTg                                                                                                                                       >  7:253797/1‑149 (MQ=255)
       cGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGa                                                                                                                                <  7:276882/149‑1 (MQ=255)
        ggTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAg                                                                                                                               >  2:298934/1‑149 (MQ=255)
         gTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCAACGAAAATTGGTGACATTACGCGTGATTTCCGCGCTGTGCTGGTGCCGAGc                                                                                                                              >  7:382732/1‑149 (MQ=255)
            ccACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcga                                                                                                                            <  2:259443/148‑1 (MQ=255)
             cACCCTGCGTGCTATCGGCCACAGTATGAGCGACGAGATCAATTATGACGCCGAAGGGCACCCGTTATCGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacg                                                                                                                          <  2:468952/149‑1 (MQ=255)
              ccccTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGGTATCGCGGGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacga                                                                                                                         <  2:190344/148‑1 (MQ=255)
               cccTGCGTGCTATCCGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGcgacga                                                                                                                         >  1:339624/1‑148 (MQ=255)
                ccTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATa                                                                                                                       <  1:298934/149‑1 (MQ=255)
                    cGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAg                                                                                                                    <  7:292519/148‑1 (MQ=255)
                         tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATa                                                                                                                       >  7:284552/1‑140 (MQ=255)
                         tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATa                                                                                                                       <  8:284552/140‑1 (MQ=255)
                         tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                                                                                              >  3:16911/1‑149 (MQ=255)
                         tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCACGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                                                                                              >  7:311950/1‑149 (MQ=255)
                                cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                       >  2:224640/1‑149 (MQ=255)
                                cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                       <  1:224640/149‑1 (MQ=255)
                                cacaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                       >  8:86082/1‑149 (MQ=255)
                                 acaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                                                                                                      <  1:24660/149‑1 (MQ=255)
                                  caGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgggg                                                                                                     <  1:242275/149‑1 (MQ=255)
                                     tATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGCCATTCCGCGTGATTTCCGCGCTGTCCTGGTGCCGAGCGACGATAAAGTCGGCCCGGTCgcggcg                                                                                                   >  2:466233/1‑148 (MQ=255)
                                       tGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGaaa                                                                                                <  6:104145/149‑1 (MQ=255)
                                               gagaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtctc                                                                                        >  8:325970/1‑149 (MQ=255)
                                                agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                       <  8:141631/149‑1 (MQ=255)
                                                agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                       <  7:86082/149‑1 (MQ=255)
                                                      tttATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc                                                                                 <  5:45627/149‑1 (MQ=255)
                                                           gACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtg                                                                             >  1:199699/1‑148 (MQ=255)
                                                            aCGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtgt                                                                            >  8:104730/1‑148 (MQ=255)
                                                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                       <  2:199699/149‑1 (MQ=255)
                                                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                       >  2:249995/1‑149 (MQ=255)
                                                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                       >  4:427736/1‑149 (MQ=255)
                                                                 gAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATgg                                                                      >  5:50896/1‑149 (MQ=255)
                                                                  aaGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgc                                                                     <  7:104730/149‑1 (MQ=255)
                                                                    ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgcg                                                                    >  3:148084/1‑148 (MQ=255)
                                                                    ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATGgcgc                                                                   <  6:50896/149‑1 (MQ=255)
                                                                     gTCACCCGTTAACGCGTGATTTACGCAGTTACGGAGCACCGAAAATTTGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGAGAAATCGATCTCGGAAATCGGTGTAAATGGCGCa                                                                  <  8:382732/149‑1 (MQ=255)
                                                                        aCCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCt                                                               >  6:371280/1‑149 (MQ=255)
                                                                           agTTTACGCTTGATTTACGCTGTTACGGGGCTCCGAAAATTGGTGACATTCCGCGTTATTTGCGCGCTGTGCTGGTGCCGAGAGACTTTAAAGTCGGCCAGTTCGGGGCGAAATAGATCTCGGAAATCGGTGTAAATTGCGCAGCTCCg                                                            <  8:253797/148‑1 (MQ=255)
                                                                             ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                          <  7:325970/149‑1 (MQ=255)
                                                                             ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                          <  4:16911/149‑1 (MQ=255)
                                                                                  gcgTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTg                                                     >  7:12191/1‑149 (MQ=255)
                                                                                       aTTTACGCAGTTACGGCGCACCGAAATTTGGTGACATTCCGCGGGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAACGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTAc                                                 <  1:466233/148‑1 (MQ=255)
                                                                                       aTTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAACTGGCGCAGCTCCGGCGATTGCTAcc                                                >  5:276895/1‑149 (MQ=255)
                                                                                        tttACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCg                                               >  2:107998/1‑149 (MQ=255)
                                                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaaa                                                                                                                     <  6:454584/74‑1 (MQ=255)
                                                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATaaa                                                                                                                     >  5:454584/1‑74 (MQ=255)
                                                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                          >  4:308569/1‑149 (MQ=255)
                                                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                          >  5:161787/1‑149 (MQ=255)
                                                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                >  3:413656/1‑92 (MQ=255)
                                                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                >  3:420284/1‑92 (MQ=255)
                                                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                <  4:413656/94‑3 (MQ=255)
                                                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                <  4:420284/94‑3 (MQ=255)
                                                                                                gTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGGGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                       <  5:219523/149‑1 (MQ=255)
                                                                                                 ttACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                       >  7:177139/1‑148 (MQ=255)
                                                                                                          aCCGAAAATTGGTGACATTCCACGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCg                             <  8:311950/149‑1 (MQ=255)
                                                                                                            cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                      <  7:326069/122‑1 (MQ=255)
                                                                                                            cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                      >  8:326069/1‑122 (MQ=255)
                                                                                                               aaaTTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                        >  8:259401/1‑149 (MQ=255)
                                                                                                                       gACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcg                >  8:256582/1‑149 (MQ=255)
                                                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc               >  6:150386/1‑149 (MQ=255)
                                                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc               >  8:340234/1‑149 (MQ=255)
                                                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc               <  7:340234/149‑1 (MQ=255)
                                                                                                                           ttCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGaa            <  8:177139/149‑1 (MQ=255)
                                                                                                                             ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt           <  3:308569/148‑1 (MQ=255)
                                                                                                                             ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGGCGGCCCGGTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCGCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt           <  2:339624/148‑1 (MQ=255)
                                                                                                                               gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg         >  3:157154/1‑148 (MQ=255)
                                                                                                                               gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg         >  4:125758/1‑148 (MQ=255)
                                                                                                                                 gTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAt      <  5:371280/149‑1 (MQ=255)
                                                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca  >  1:445866/1‑149 (MQ=255)
                                                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca  <  6:161787/149‑1 (MQ=255)
                                                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGAGGCATGCGGCATCTGGTTACGCGAATGGCATTTca  <  6:276895/149‑1 (MQ=255)
                                                                                                                                      ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                      <  3:404131/96‑1 (MQ=255)
                                                                                                                                      ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                      >  4:404131/1‑96 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
AAATCTACGGTGCCACCCTGCGTGCTATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCA  >  NC_000913/3023867‑3024148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: