Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,024,038 G→C 5.7% G908A (GGC→GCC)  xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,024,0380GC 5.7% 233.6 / 5.5 70G908A (GGC→GCC) xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base G (36/30);  new base C (2/2);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01

TATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAA  >  NC_000913/3023892‑3024186
                                                                                                                                                  |                                                                                                                                                    
tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                                                                                                                                    >  3:16911/1‑149 (MQ=255)
tATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCACGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGcc                                                                                                                                                    >  7:311950/1‑149 (MQ=255)
       cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                             <  1:224640/149‑1 (MQ=255)
       cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                             >  2:224640/1‑149 (MQ=255)
       cacaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                             >  8:86082/1‑149 (MQ=255)
        acaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                                                                                                                                            <  1:24660/149‑1 (MQ=255)
         caGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgggg                                                                                                                                           <  1:242275/149‑1 (MQ=255)
            tATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGCCATTCCGCGTGATTTCCGCGCTGTCCTGGTGCCGAGCGACGATAAAGTCGGCCCGGTCgcggcg                                                                                                                                         >  2:466233/1‑148 (MQ=255)
              tGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGaaa                                                                                                                                      <  6:104145/149‑1 (MQ=255)
                      gagaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtctc                                                                                                                              >  8:325970/1‑149 (MQ=255)
                       agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                                                             <  8:141631/149‑1 (MQ=255)
                       agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                                                             <  7:86082/149‑1 (MQ=255)
                             tttATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc                                                                                                                       <  5:45627/149‑1 (MQ=255)
                                  gACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtg                                                                                                                   >  1:199699/1‑148 (MQ=255)
                                   aCGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtgt                                                                                                                  >  8:104730/1‑148 (MQ=255)
                                       cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                             >  2:249995/1‑149 (MQ=255)
                                       cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                             <  2:199699/149‑1 (MQ=255)
                                       cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                             >  4:427736/1‑149 (MQ=255)
                                        gAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATgg                                                                                                            >  5:50896/1‑149 (MQ=255)
                                         aaGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgc                                                                                                           <  7:104730/149‑1 (MQ=255)
                                           ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgcg                                                                                                          >  3:148084/1‑148 (MQ=255)
                                           ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATGgcgc                                                                                                         <  6:50896/149‑1 (MQ=255)
                                            gTCACCCGTTAACGCGTGATTTACGCAGTTACGGAGCACCGAAAATTTGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGAGAAATCGATCTCGGAAATCGGTGTAAATGGCGCa                                                                                                        <  8:382732/149‑1 (MQ=255)
                                               aCCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCt                                                                                                     >  6:371280/1‑149 (MQ=255)
                                                  agTTTACGCTTGATTTACGCTGTTACGGGGCTCCGAAAATTGGTGACATTCCGCGTTATTTGCGCGCTGTGCTGGTGCCGAGAGACTTTAAAGTCGGCCAGTTCGGGGCGAAATAGATCTCGGAAATCGGTGTAAATTGCGCAGCTCCg                                                                                                  <  8:253797/148‑1 (MQ=255)
                                                    ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                                                                <  7:325970/149‑1 (MQ=255)
                                                    ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                                                                <  4:16911/149‑1 (MQ=255)
                                                         gcgTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTg                                                                                           >  7:12191/1‑149 (MQ=255)
                                                              aTTTACGCAGTTACGGCGCACCGAAATTTGGTGACATTCCGCGGGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAACGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTAc                                                                                       <  1:466233/148‑1 (MQ=255)
                                                              aTTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAACTGGCGCAGCTCCGGCGATTGCTAcc                                                                                      >  5:276895/1‑149 (MQ=255)
                                                               tttACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCg                                                                                     >  2:107998/1‑149 (MQ=255)
                                                                    gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                                                                >  4:308569/1‑149 (MQ=255)
                                                                    gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                                                                >  5:161787/1‑149 (MQ=255)
                                                                     cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                      >  3:420284/1‑92 (MQ=255)
                                                                     cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                      >  3:413656/1‑92 (MQ=255)
                                                                     cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                      <  4:420284/94‑3 (MQ=255)
                                                                     cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                      <  4:413656/94‑3 (MQ=255)
                                                                       gTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGGGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                                                             <  5:219523/149‑1 (MQ=255)
                                                                        ttACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                                                             >  7:177139/1‑148 (MQ=255)
                                                                                 aCCGAAAATTGGTGACATTCCACGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCg                                                                   <  8:311950/149‑1 (MQ=255)
                                                                                   cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                            <  7:326069/122‑1 (MQ=255)
                                                                                   cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                            >  8:326069/1‑122 (MQ=255)
                                                                                      aaaTTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                              >  8:259401/1‑149 (MQ=255)
                                                                                              gACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcg                                                      >  8:256582/1‑149 (MQ=255)
                                                                                               aCATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                     >  6:150386/1‑149 (MQ=255)
                                                                                               aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                     <  7:340234/149‑1 (MQ=255)
                                                                                               aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                     >  8:340234/1‑149 (MQ=255)
                                                                                                  ttCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGaa                                                  <  8:177139/149‑1 (MQ=255)
                                                                                                    ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt                                                 <  3:308569/148‑1 (MQ=255)
                                                                                                    ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGGCGGCCCGGTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCGCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt                                                 <  2:339624/148‑1 (MQ=255)
                                                                                                      gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                               >  4:125758/1‑148 (MQ=255)
                                                                                                      gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                               >  3:157154/1‑148 (MQ=255)
                                                                                                        gTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAt                                            <  5:371280/149‑1 (MQ=255)
                                                                                                            tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                        <  6:161787/149‑1 (MQ=255)
                                                                                                            tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                        >  1:445866/1‑149 (MQ=255)
                                                                                                            tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGAGGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                        <  6:276895/149‑1 (MQ=255)
                                                                                                             ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                            <  3:404131/96‑1 (MQ=255)
                                                                                                             ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                            >  4:404131/1‑96 (MQ=255)
                                                                                                                              gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                              <  1:304005/109‑1 (MQ=255)
                                                                                                                              gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                              >  2:304005/1‑109 (MQ=255)
                                                                                                                              gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCa                      <  1:249995/149‑1 (MQ=255)
                                                                                                                                    cgacgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTgcgc                >  5:431158/1‑149 (MQ=255)
                                                                                                                                     gacgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCt               >  1:188451/1‑149 (MQ=255)
                                                                                                                                      acgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAtt                                           >  3:423408/1‑120 (MQ=255)
                                                                                                                                      acgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAtt                                           <  4:423408/120‑1 (MQ=255)
                                                                                                                                          tAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCGATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaa           >  2:171357/1‑148 (MQ=255)
                                                                                                                                           aaaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaaa          >  8:142790/1‑148 (MQ=255)
                                                                                                                                            aaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTc                                         >  3:404888/1‑116 (MQ=255)
                                                                                                                                            aaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTc                                         <  4:404888/116‑1 (MQ=255)
                                                                                                                                              gTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaaaaaa       >  1:317946/1‑148 (MQ=255)
                                                                                                                                                 ggCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAatat    <  1:462851/148‑1 (MQ=255)
                                                                                                                                                  gCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATataa  <  5:379714/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TATCGGCCACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAA  >  NC_000913/3023892‑3024186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: