Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,024,043 T→A 5.3% F910I (TTC→ATC) ‡ xdhD → putative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,024,0430TA 5.3% 238.3 / 3.4 76F910I (TTC→ATC) ‡xdhDputative hypoxanthine oxidase, molybdopterin‑binding/Fe‑S binding
Reads supporting (aligned to +/- strand):  ref base T (36/34);  new base A (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

CACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAAATAAT  >  NC_000913/3023899‑3024191
                                                                                                                                                |                                                                                                                                                    
cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                                  <  1:224640/149‑1 (MQ=255)
cacaGTATGAGCGAAGAGATCATTTATGACGCTGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                                  >  2:224640/1‑149 (MQ=255)
cacaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgg                                                                                                                                                  >  8:86082/1‑149 (MQ=255)
 acaGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCggg                                                                                                                                                 <  1:24660/149‑1 (MQ=255)
  caGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCgggg                                                                                                                                                <  1:242275/149‑1 (MQ=255)
     tATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGCCATTCCGCGTGATTTCCGCGCTGTCCTGGTGCCGAGCGACGATAAAGTCGGCCCGGTCgcggcg                                                                                                                                              >  2:466233/1‑148 (MQ=255)
       tGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGaaa                                                                                                                                           <  6:104145/149‑1 (MQ=255)
               gagaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAtctc                                                                                                                                   >  8:325970/1‑149 (MQ=255)
                agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                                                                  <  8:141631/149‑1 (MQ=255)
                agaTCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCg                                                                                                                                  <  7:86082/149‑1 (MQ=255)
                      tttATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATc                                                                                                                            <  5:45627/149‑1 (MQ=255)
                           gACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtg                                                                                                                        >  1:199699/1‑148 (MQ=255)
                            aCGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGgtgt                                                                                                                       >  8:104730/1‑148 (MQ=255)
                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                                  <  2:199699/149‑1 (MQ=255)
                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                                  >  4:427736/1‑149 (MQ=255)
                                cGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATg                                                                                                                  >  2:249995/1‑149 (MQ=255)
                                 gAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATgg                                                                                                                 >  5:50896/1‑149 (MQ=255)
                                  aaGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgc                                                                                                                <  7:104730/149‑1 (MQ=255)
                                    ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGgcg                                                                                                               >  3:148084/1‑148 (MQ=255)
                                    ggTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGAGCTCGGAAATCGGTGTAAATGgcgc                                                                                                              <  6:50896/149‑1 (MQ=255)
                                     gTCACCCGTTAACGCGTGATTTACGCAGTTACGGAGCACCGAAAATTTGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGAGAAATCGATCTCGGAAATCGGTGTAAATGGCGCa                                                                                                             <  8:382732/149‑1 (MQ=255)
                                        aCCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCt                                                                                                          >  6:371280/1‑149 (MQ=255)
                                           agTTTACGCTTGATTTACGCTGTTACGGGGCTCCGAAAATTGGTGACATTCCGCGTTATTTGCGCGCTGTGCTGGTGCCGAGAGACTTTAAAGTCGGCCAGTTCGGGGCGAAATAGATCTCGGAAATCGGTGTAAATTGCGCAGCTCCg                                                                                                       <  8:253797/148‑1 (MQ=255)
                                             ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                                                                     <  7:325970/149‑1 (MQ=255)
                                             ttAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGc                                                                                                     <  4:16911/149‑1 (MQ=255)
                                                  gcgTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTg                                                                                                >  7:12191/1‑149 (MQ=255)
                                                       aTTTACGCAGTTACGGCGCACCGAAATTTGGTGACATTCCGCGGGATTTCCGCGCTGTGCTGGGGCCGAGCGACGATAACGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTAc                                                                                            <  1:466233/148‑1 (MQ=255)
                                                       aTTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAACTGGCGCAGCTCCGGCGATTGCTAcc                                                                                           >  5:276895/1‑149 (MQ=255)
                                                        tttACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCg                                                                                          >  2:107998/1‑149 (MQ=255)
                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                                                                     >  5:161787/1‑149 (MQ=255)
                                                             gCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAAtt                                                                                     >  4:308569/1‑149 (MQ=255)
                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                           <  4:420284/94‑3 (MQ=255)
                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                           <  4:413656/94‑3 (MQ=255)
                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                           >  3:413656/1‑92 (MQ=255)
                                                              cAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCAGGTGCCGAGCGACGATAAAGTCGCCCCGAACGGGCCGact                                                                                                                                           >  3:420284/1‑92 (MQ=255)
                                                                gTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGGGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                                                                  <  5:219523/149‑1 (MQ=255)
                                                                 ttACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCAc                                                                                  >  7:177139/1‑148 (MQ=255)
                                                                          aCCGAAAATTGGTGACATTCCACGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCg                                                                        <  8:311950/149‑1 (MQ=255)
                                                                            cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                                 >  8:326069/1‑122 (MQ=255)
                                                                            cGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                                 <  7:326069/122‑1 (MQ=255)
                                                                               aaaTTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                                   >  8:259401/1‑149 (MQ=255)
                                                                                       gACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcg                                                           >  8:256582/1‑149 (MQ=255)
                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                          >  6:150386/1‑149 (MQ=255)
                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                          >  8:340234/1‑149 (MQ=255)
                                                                                        aCATTCCGCGTGATTTCCGCGCTGTGCTAGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTAcgc                                                          <  7:340234/149‑1 (MQ=255)
                                                                                           ttCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGaa                                                       <  8:177139/149‑1 (MQ=255)
                                                                                             ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt                                                      <  3:308569/148‑1 (MQ=255)
                                                                                             ccGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGGCGGCCCGGTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCGCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAAt                                                      <  2:339624/148‑1 (MQ=255)
                                                                                               gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                                    >  4:125758/1‑148 (MQ=255)
                                                                                               gcgTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                                    >  3:157154/1‑148 (MQ=255)
                                                                                                 gTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAt                                                 <  5:371280/149‑1 (MQ=255)
                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                             >  1:445866/1‑149 (MQ=255)
                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                             <  6:161787/149‑1 (MQ=255)
                                                                                                     tttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGAGGCATGCGGCATCTGGTTACGCGAATGGCATTTca                                             <  6:276895/149‑1 (MQ=255)
                                                                                                      ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                                 <  3:404131/96‑1 (MQ=255)
                                                                                                      ttCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGAtt                                                                                                 >  4:404131/1‑96 (MQ=255)
                                                                                                                       gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                                   <  1:304005/109‑1 (MQ=255)
                                                                                                                       gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATc                                                                   >  2:304005/1‑109 (MQ=255)
                                                                                                                       gCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCa                           <  1:249995/149‑1 (MQ=255)
                                                                                                                             cgacgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTgcgc                     >  5:431158/1‑149 (MQ=255)
                                                                                                                              gacgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCt                    >  1:188451/1‑149 (MQ=255)
                                                                                                                               acgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAtt                                                >  3:423408/1‑120 (MQ=255)
                                                                                                                               acgaTAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCAtt                                                <  4:423408/120‑1 (MQ=255)
                                                                                                                                   tAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCGATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaa                >  2:171357/1‑148 (MQ=255)
                                                                                                                                    aaaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaaa               >  8:142790/1‑148 (MQ=255)
                                                                                                                                     aaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTc                                              >  3:404888/1‑116 (MQ=255)
                                                                                                                                     aaGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTc                                              <  4:404888/116‑1 (MQ=255)
                                                                                                                                       gTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGaaaaaa            >  1:317946/1‑148 (MQ=255)
                                                                                                                                          ggCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAatat         <  1:462851/148‑1 (MQ=255)
                                                                                                                                           gCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATataa       <  5:379714/149‑1 (MQ=255)
                                                                                                                                             ccGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                                    >  5:240181/1‑102 (MQ=255)
                                                                                                                                             ccGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATgg                                                    <  6:240181/102‑1 (MQ=255)
                                                                                                                                             ccGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATataaa      >  6:282190/1‑148 (MQ=255)
                                                                                                                                              cGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAatat         <  4:18859/144‑1 (MQ=255)
                                                                                                                                              cGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATataagat    >  3:18859/1‑146 (MQ=255)
                                                                                                                                               gTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATataaataa   <  3:427736/149‑1 (MQ=255)
                                                                                                                                                ttCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAaataat  <  7:142790/149‑1 (MQ=255)
                                                                                                                                                ttCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAaataat  <  8:12191/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CACAGTATGAGCGAAGAGATCATTTATGACGCCGAAGGTCACCCGTTAACGCGTGATTTACGCAGTTACGGCGCACCGAAAATTGGTGACATTCCGCGTGATTTCCGCGCTGTGCTGGTGCCGAGCGACGATAAAGTCGGCCCGTTCGGGGCGAAATCGATCTCGGAAATCGGTGTAAATGGCGCAGCTCCGGCGATTGCTACCGCAATTCACGATGCATGCGGCATCTGGTTACGCGAATGGCATTTCACACCGGAGAAAATACTCACTGCGCTGGAAAAAATATAAATAAT  >  NC_000913/3023899‑3024191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: