Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 194,737 A→T 6.3% intergenic (+20/‑166) dxr → / → ispU 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl pyrophosphate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913194,7370AT 6.3% 257.3 / 3.8 83intergenic (+20/‑166)dxr/ispU1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl pyrophosphate synthase
Reads supporting (aligned to +/- strand):  ref base A (33/43);  new base T (2/2);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

GCTGCGTTGAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCT  >  NC_000913/194589‑194883
                                                                                                                                                    |                                                                                                                                                  
gCTGCGTTGAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACagaga                                                                                                                                                    <  1:287331/149‑1 (MQ=255)
     gTTGAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTcgc                                                                                                                                               <  8:198609/149‑1 (MQ=255)
     gTTGAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATCCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTcgc                                                                                                                                               >  8:455394/1‑149 (MQ=255)
        gAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCgcgc                                                                                                                                             <  1:172490/148‑1 (MQ=255)
         aaTTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCt                                                                                                                                            <  5:413439/148‑1 (MQ=255)
          aTTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTAGAGAGGTTCGCGCTAt                                                                                                                                          >  5:71715/1‑149 (MQ=255)
            ttATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTAttt                                                                                                                                        >  5:22566/1‑149 (MQ=255)
               tCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGtt                                                                                                                                     <  3:250462/149‑1 (MQ=255)
                  gTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGc                                                                                                                                  <  5:326606/149‑1 (MQ=255)
                  gTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGc                                                                                                                                  <  1:205908/149‑1 (MQ=255)
                     cTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGt                                                                                                                                <  1:279599/148‑1 (MQ=255)
                      tGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATTCGAACGAGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAg                                                                                                                              <  4:128534/149‑1 (MQ=255)
                        gAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAggg                                                                                                                            >  3:225493/1‑149 (MQ=255)
                          aaaaaTGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAAGCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCt                                                                                                                          <  1:96853/149‑1 (MQ=255)
                            aaaTGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTc                                                                                                                        <  1:372543/149‑1 (MQ=255)
                             aaTGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCa                                                                                                                       <  1:323005/149‑1 (MQ=255)
                              aTGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAg                                                                                                                      >  5:57323/1‑149 (MQ=255)
                               tGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGt                                                                                                                     <  3:193307/149‑1 (MQ=255)
                                     tGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGGTTCAGTGata                                                                                                                 >  6:112821/1‑147 (MQ=255)
                                         cgAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCt                                                                                                           <  8:331512/149‑1 (MQ=255)
                                              ccctaTGTGTGGACGATGTGTTATCTGTTGATGAGAACGCGCGTGAAGTAGACAGAAAAGATGTGATGAGTCTCGAAAGCTACGGATAATCCGGCTACTGAGAGTCGCGCTATGTGTTAGCGTAGGGCTTAAGTGata                                                                                                                 >  7:329136/5‑138 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGc                                                                                                                           >  2:177173/1‑128 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGc                                                                                                                           <  1:177173/128‑1 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGata                                                                                                                 >  1:433617/1‑138 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTgcgc                                                                                                       >  2:108342/1‑148 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTgcgc                                                                                                       >  2:140779/1‑148 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTgcgc                                                                                                       >  6:160604/1‑148 (MQ=255)
                                              cacaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAATCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTgcgc                                                                                                       >  8:107473/1‑148 (MQ=255)
                                              cacaATGTGGGGACGATGTTTTATCTGTTTATGCTAACGCGCGTGAAGTCGCCAGAAACCAGGTGATGCGTCTCGCAAGCTGAGGATACTCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGata                                                                                                                 <  8:329136/138‑1 (MQ=255)
                                               acaATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGGGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCa                                                                                                     <  6:71715/149‑1 (MQ=255)
                                                 aaTGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATc                                                                                                   >  5:171109/1‑149 (MQ=255)
                                                     tgtgGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGAt                                                                                               <  5:160604/149‑1 (MQ=255)
                                                       tgGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATc                                                                                              <  4:225493/148‑1 (MQ=255)
                                                          aCGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGt                                                                                            >  7:381794/1‑147 (MQ=255)
                                                          aCGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTaa                                                                                          <  1:140779/149‑1 (MQ=255)
                                                                tgtTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGtt                                                                                    <  1:108342/149‑1 (MQ=255)
                                                                  ttATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTgg                                                                                  >  1:278129/1‑149 (MQ=255)
                                                                    aTCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGATGAGGATAATCCTGCTACAGAGAGTCGCGCTATTTGTTAGCGTATGGCTTCAGTGATATAGTCTGCGCCATCTGATAGGAAGTAGtttgat                                                                                >  6:31483/1‑145 (MQ=255)
                                                                         ttGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGGAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTtat                                                                            >  7:352552/1‑148 (MQ=255)
                                                                           gATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAg                                                                         >  1:330316/1‑149 (MQ=255)
                                                                             tGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAg                                                                                                                      >  3:54863/1‑102 (MQ=255)
                                                                             tGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAg                                                                                                                      <  4:54863/102‑1 (MQ=255)
                                                                             tGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGt                                                                       <  4:170528/149‑1 (MQ=255)
                                                                                gAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGt                                                                       >  2:12191/1‑146 (MQ=255)
                                                                                     cgcgTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGGCAGATATg                                                               <  6:57323/149‑1 (MQ=255)
                                                                                        gTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCg                                                            >  8:110996/1‑149 (MQ=255)
                                                                                         tGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGt                                                           >  4:350474/1‑149 (MQ=255)
                                                                                          gAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAag                                                                                         <  4:253006/118‑1 (MQ=255)
                                                                                          gAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAag                                                                                         >  3:253006/1‑118 (MQ=255)
                                                                                           aaGGCGCCAGATAAGAGTTGAGGCGTCTCGCAAGCTGAGGATAATCCGTCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGTTATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAATGTCAGATATGCCGTgg                                                         <  5:406373/149‑1 (MQ=255)
                                                                                             gTCGCCAGAAAAGATGTTATGCGTCTCTCAAGCTGATGATAATCAGGCTTCATAGAGTAGCTCTATTTGTTAGCGTATGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTAAGATATGCCGTGGtt                                                       <  3:58950/149‑1 (MQ=255)
                                                                                                gCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTa                                                    >  2:115717/1‑149 (MQ=255)
                                                                                                     aaaaGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACgg                                               >  7:422848/1‑149 (MQ=255)
                                                                                                            gTGCTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTCGCGTAGGACTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttt                                        <  4:123975/149‑1 (MQ=255)
                                                                                                            gTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGCGTCGCGCTATTTGTTAGCGTAGGACTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttt                                        <  4:129779/149‑1 (MQ=255)
                                                                                                              gATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      >  6:49067/1‑149 (MQ=255)
                                                                                                              gATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  6:22566/149‑1 (MQ=255)
                                                                                                               aTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  1:115717/148‑1 (MQ=255)
                                                                                                               aTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  3:350474/148‑1 (MQ=255)
                                                                                                               aTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  2:330316/148‑1 (MQ=255)
                                                                                                               aTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  2:278129/148‑1 (MQ=255)
                                                                                                               aTGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAATCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  7:107473/148‑1 (MQ=255)
                                                                                                                 gCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACCCGGCTTTTTTTTGta                                   >  6:250347/1‑149 (MQ=255)
                                                                                                                  cGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGtat                                  <  8:381794/149‑1 (MQ=255)
                                                                                                                   gTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGtata                                 <  6:171109/149‑1 (MQ=255)
                                                                                                                   gTCTAGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCTTGGTTTTACACGGCTTTTTTTTGtata                                 <  5:31483/149‑1 (MQ=255)
                                                                                                                      tcGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      <  2:63983/141‑1 (MQ=255)
                                                                                                                      tcGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtttttttt                                      >  1:63983/1‑141 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGTGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAg                                                                    <  5:124590/109‑1 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGTGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAg                                                                    <  5:130722/109‑1 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGTGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAg                                                                    >  6:130722/1‑109 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGTGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAg                                                                    >  6:124590/1‑109 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCtt                            <  2:418452/149‑1 (MQ=255)
                                                                                                                        gCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCt                             >  1:418452/1‑148 (MQ=255)
                                                                                                                           aGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAg                         <  1:12191/149‑1 (MQ=255)
                                                                                                                           aGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAg                         <  7:110996/149‑1 (MQ=255)
                                                                                                                             cTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtt                                            >  6:384851/1‑128 (MQ=255)
                                                                                                                             cTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCtt                                            <  5:384851/128‑1 (MQ=255)
                                                                                                                                aGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTc                    >  2:422325/1‑149 (MQ=255)
                                                                                                                                aGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTc                    <  5:112821/149‑1 (MQ=255)
                                                                                                                                  gATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCt                  <  7:455394/149‑1 (MQ=255)
                                                                                                                                  gATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCt                  >  3:259598/1‑149 (MQ=255)
                                                                                                                                  gATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCt                  <  4:259598/149‑1 (MQ=255)
                                                                                                                                       tCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTGAGATATGCCGTGGTGTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTa             >  8:435300/1‑149 (MQ=255)
                                                                                                                                                  agagTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTACTGAGTACCGTAAACCCt  >  4:263285/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCTGCGTTGAATTTATCCGTACTGGAAAAAATGGATATGCGCGAACCACAATGTGTGGACGATGTGTTATCTGTTGATGCGAACGCGCGTGAAGTCGCCAGAAAAGAGGTGATGCGTCTCGCAAGCTGAGGATAATCCGGCTACAGAGAGTCGCGCTATTTGTTAGCGTAGGGCTTCAGTGATATAGTCTGCGCCATCTGATCGTAAGTAGTTGGCTTTATAAGGTCAGATATGCCGTGGTTTTACACGGCTTTTTTTTGTATAGGCTTCAGTATTCCTGAGTACCGTAAACCCT  >  NC_000913/194589‑194883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: