Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,357,939 G→A 5.8% G1072D (GGC→GAC)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,357,9390GA 5.8% 274.0 / 4.7 86G1072D (GGC→GAC) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (36/45);  new base A (2/3);  total (38/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

GCAGGCTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCA  >  NC_000913/3357794‑3358083
                                                                                                                                                 |                                                                                                                                                
gCAGGCTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCt                                                                                                                                               >  4:390101/1‑149 (MQ=255)
 cAGGCTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCt                                                                                                                                               >  1:199380/1‑148 (MQ=255)
  aGGCTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCATCACAAGATCCGTTTGCAGGTCGATGGCGGCCTg                                                                                                                                              >  4:181152/1‑148 (MQ=255)
     cTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGaaaa                                                                                                                                          >  6:163412/1‑149 (MQ=255)
        tGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACg                                                                                                                                        <  3:79687/148‑1 (MQ=255)
          aCGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTTCAGGCCGCCATCGACCTGCAAACggat                                                                                                                                     <  7:256409/149‑3 (MQ=255)
          aCGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACggg                                                                                                                                      <  2:160938/148‑1 (MQ=255)
          aCGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCGGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgt                                                                                                                                     <  7:276167/149‑1 (MQ=255)
          aCGGCGGCACCGGCGCAAGTCCGCTGTCATCGGTTAAATACGCCGGCTGTCCGGGGGAGCTGGGTCGTGTTGCAACCCAGCAGGCGCGGGTTGCTAACGTCTTGCGTCACAAGATCCGTTTTCAGGCCGCCATCGACCTGCAAACgga                                                                                                                                      <  7:328005/148‑2 (MQ=255)
            ggcggcACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgtgt                                                                                                                                   >  4:174280/1‑149 (MQ=255)
               ggcACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGa                                                                                                                                <  6:181520/149‑1 (MQ=255)
                  aCCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGatat                                                                                                                             >  2:170139/1‑149 (MQ=255)
                   ccGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATatc                                                                                                                            <  5:124463/149‑1 (MQ=255)
                   ccGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATatc                                                                                                                            <  7:167902/149‑1 (MQ=255)
                      gcgcAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATatcat                                                                                                                          >  7:322081/1‑148 (MQ=255)
                       cgcAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATAtcatca                                                                                                                        <  4:190029/149‑1 (MQ=255)
                        gcAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCaa                                                                                                                       <  2:191515/149‑1 (MQ=255)
                             tCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAggcgg                                                                                                                  <  7:404544/149‑1 (MQ=255)
                               cGCCTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTTCAGGCCGCCATCGACCTGCAAACggatc                                                                                                                                    <  8:193469/129‑4 (MQ=37)
                               cGCCTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTTCAGGCCGCCATCGACCTGCAAACggatc                                                                                                                                    >  7:193469/1‑126 (MQ=37)
                                gCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGa                                                                                                               >  8:289106/1‑149 (MQ=255)
                                gCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGa                                                                                                               >  4:348082/1‑149 (MQ=255)
                                gCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCGGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGa                                                                                                               <  1:189882/149‑1 (MQ=255)
                                  tttCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAGACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGAtt                                                                                                             >  3:148460/1‑149 (MQ=255)
                                      aTCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCg                                                                                                         >  5:141381/1‑149 (MQ=255)
                                       tCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCgg                                                                                                        <  3:174280/149‑1 (MQ=255)
                                       tCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCgg                                                                                                        >  3:425425/1‑149 (MQ=255)
                                       tCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCgg                                                                                                        <  1:328495/149‑1 (MQ=255)
                                        cGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGgc                                                                                                       >  4:151804/1‑149 (MQ=255)
                                              aaTACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGaa                                                                                                 <  7:186351/149‑1 (MQ=255)
                                               aTACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGaaa                                                                                                <  8:238848/149‑1 (MQ=255)
                                                   gCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATtctc                                                                                                          >  7:389704/1‑135 (MQ=255)
                                                   gCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATtctc                                                                                                          <  8:389704/135‑1 (MQ=255)
                                                   gCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgctt                                                                                            >  2:266502/1‑149 (MQ=255)
                                                   gCAGGCTGTCCGTGGGAGCTGGGGCGTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgctt                                                                                            <  6:351349/149‑1 (MQ=255)
                                                      ggCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgcttcgg                                                                                         >  4:276752/1‑149 (MQ=255)
                                                       gCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgcttcggc                                                                                        <  1:266502/149‑1 (MQ=255)
                                                           tCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgcttcggcttcg                                                                                    <  8:359659/149‑1 (MQ=255)
                                                                 ggAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTg                                                                              <  7:289106/149‑1 (MQ=255)
                                                                        gggCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgt                                                                                                                                     <  6:393143/87‑1 (MQ=255)
                                                                        gggCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgt                                                                                                                                     >  5:393143/1‑87 (MQ=255)
                                                                        gggCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAt                                                                       <  6:62668/149‑1 (MQ=255)
                                                                         ggCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCATCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtg                                                                      <  3:181152/149‑1 (MQ=255)
                                                                         ggCGTGTTGAAACCCTGCAGGCGCGGGTTGCTCACGGCGTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCAGCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtg                                                                      <  5:158038/149‑1 (MQ=255)
                                                                          gCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtgg                                                                     >  1:176605/1‑149 (MQ=255)
                                                                              gttgAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtggtg                                                                   <  3:348082/147‑1 (MQ=255)
                                                                              gttgAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtggtg                                                                   <  3:151804/147‑1 (MQ=255)
                                                                              gttgAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtggtg                                                                   <  7:196200/147‑1 (MQ=255)
                                                                              gttgAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGACCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGgc                                                                 >  1:336593/1‑149 (MQ=255)
                                                                                   aaCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGgc                                                                                                       >  3:118127/1‑106 (MQ=255)
                                                                                   aaCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGgc                                                                                                       <  4:118127/106‑1 (MQ=255)
                                                                                   aaCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCg                                                            <  8:322081/149‑1 (MQ=255)
                                                                                    aCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtggtg                                                                   <  5:366058/141‑1 (MQ=255)
                                                                                    aCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGAtggtg                                                                   >  6:366058/1‑141 (MQ=255)
                                                                                    aCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCgg                                                           >  3:424921/1‑149 (MQ=255)
                                                                                    aCCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCgg                                                           <  4:12779/149‑1 (MQ=255)
                                                                                      ccAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgcttcggcttc                                                                                     <  1:342380/121‑1 (MQ=255)
                                                                                      ccAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAgcttcggcttc                                                                                     >  2:342380/1‑121 (MQ=255)
                                                                                       cagcagGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTg                                                        >  3:334750/1‑149 (MQ=255)
                                                                                       cagcagGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTg                                                        >  4:203271/1‑149 (MQ=255)
                                                                                        agcagGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGt                                                       <  3:390101/149‑1 (MQ=255)
                                                                                                cTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCt                                               <  1:170139/149‑1 (MQ=255)
                                                                                                   gTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTAc                                             <  4:148460/148‑1 (MQ=255)
                                                                                                   gTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACg                                            <  3:276752/149‑1 (MQ=255)
                                                                                                        tAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgtgt                                                                                                                                   <  5:105909/57‑1 (MQ=255)
                                                                                                        tAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGgtgt                                                                                                                                   >  6:105909/1‑57 (MQ=255)
                                                                                                        tAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTg                                                        <  8:129529/132‑1 (MQ=255)
                                                                                                        tAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTg                                                        >  7:129529/1‑132 (MQ=255)
                                                                                                        tAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTAttt                                       >  6:19144/1‑149 (MQ=255)
                                                                                                         aaCGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGTTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTAGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTg                                      >  7:128804/1‑149 (MQ=255)
                                                                                                         aaCGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCt                                               >  3:158468/1‑140 (MQ=255)
                                                                                                         aaCGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCt                                               <  4:158468/140‑1 (MQ=255)
                                                                                                                 gCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTg                               >  5:244542/1‑148 (MQ=255)
                                                                                                                     cacaAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAATCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGaacaa                          >  6:289259/1‑149 (MQ=255)
                                                                                                                        aaGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTg                       <  4:334750/149‑1 (MQ=255)
                                                                                                                           aTCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTgcg                     >  1:446136/1‑148 (MQ=255)
                                                                                                                            tCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATc                                 <  4:101350/135‑1 (MQ=255)
                                                                                                                            tCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATc                                 >  3:101350/1‑135 (MQ=255)
                                                                                                                              cGTTTGCAGGTCGTTTGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGGACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAAc                 <  2:199380/149‑1 (MQ=255)
                                                                                                                                  tGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACggg              <  6:141381/148‑1 (MQ=255)
                                                                                                                                   gCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGgt             <  4:424921/148‑1 (MQ=255)
                                                                                                                                       gTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGTCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTa          <  1:145605/147‑1 (MQ=255)
                                                                                                                                       gTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTa          >  2:145605/1‑147 (MQ=255)
                                                                                                                                        tCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGc        >  5:193667/1‑148 (MQ=255)
                                                                                                                                          gATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAAc     <  5:19144/149‑1 (MQ=255)
                                                                                                                                           aTGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACt    >  3:70143/1‑149 (MQ=255)
                                                                                                                                              gcggcCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCa  <  3:203271/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCAGGCTATGACGGCGGCACCGGCGCAAGTCCGCTTTCATCGGTGAAATACGCAGGCTGTCCGTGGGAGCTGGGGCTTGTTGAAACCCAGCAGGCGCTGGTTGCTAACGGCTTGCGTCACAAGATCCGTTTGCAGGTCGATGGCGGCCTGAAAACGGGTGTCGATATCATCAAGGCGGCGATTCTCGGCGCAGAAAGCTTCGGCTTCGGCACTGGCCCGATGGTGGCGCTCGGCTGTAAATATCTACGTATTTGCCATCTGAACAACTGCGCAACGGGTGTAGCAACTCA  >  NC_000913/3357794‑3358083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: