Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,647,672 G→C 5.1% intergenic (+21/+33) gor → / ← dinQ glutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,647,6720GC 5.1% 269.5 / 5.3 79intergenic (+21/+33)gor/dinQglutathione oxidoreductase/UV‑inducible membrane toxin, DinQ‑AgrB type I toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (42/33);  new base C (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

CTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAT  >  NC_000913/3647525‑3647817
                                                                                                                                                   |                                                                                                                                                 
cTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGagtagt                                                                                                                                                  <  7:260436/149‑1 (MQ=255)
      tATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                            <  4:237477/149‑1 (MQ=255)
        tGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATACACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctct                                                                                                                                           >  6:350461/1‑148 (MQ=255)
         ggACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctct                                                                                                                                           >  6:194130/1‑147 (MQ=255)
           aCGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGc                                                                                                                                       >  5:156416/1‑149 (MQ=255)
                 agatGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGACGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCGAAGAGTAGTGTGCTCTTAGCCCTTaa                                                                                                                                 <  4:317069/146‑1 (MQ=255)
                     gCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTAc                                                                                                                             >  7:364934/1‑149 (MQ=255)
                      cAGGGCTTCGCGGTGGCGCTGAAGATGGGCGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTAc                                                                                                                             <  5:350461/148‑1 (MQ=255)
                        gggCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGATAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGtt                                                                                                                          <  1:147027/149‑1 (MQ=255)
                         ggCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCGAAGAGTAGTGTGCTCTTAGCCCTTAATTACGttt                                                                                                                         >  2:442232/1‑149 (MQ=255)
                           cTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTcc                                                                                                                       <  1:442232/149‑1 (MQ=255)
                              cgcgGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGc                                                                                                                     <  5:194130/148‑1 (MQ=255)
                                       gcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTTAAGGGCTAAGAGCACACTACTCTTAGCCCtttaa                                                                                                                                >  3:118890/1‑125 (MQ=38)
                                       gcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTTAAGGGCTAAGAGCACACTACTCTTAGCCCtttaa                                                                                                                                <  4:118890/128‑4 (MQ=38)
                                       gcTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGtt                                                                                                             >  8:453879/1‑147 (MQ=255)
                                        cTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAg                                                                                                          <  8:364934/149‑1 (MQ=255)
                                           aaGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGaa                                                                                                        <  1:347333/148‑1 (MQ=255)
                                                gggggCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTg                                                                                                    >  1:15141/1‑147 (MQ=255)
                                                 ggggCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGaa                                                                                                  <  7:453879/148‑1 (MQ=255)
                                                    gCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAg                                                                                                 >  5:445581/1‑146 (MQ=255)
                                                      aaCCAAAAAATACTTCGACTATTCCTTCGCGATTCACCCAACGGCGGGAGTAGTTTTCGTGACAATGCGTTATATGTTAAAGGTCTAAGTGTAGTGTGCTCTTAGCCCTTAATTACGTTTc                                                                                                                        <  2:258340/121‑1 (MQ=255)
                                                      aaCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGa                                                                                             >  7:146703/1‑148 (MQ=255)
                                                      aaCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAAAGCGTTAAATGTTAAAGGGCTAAGAGTAGAGTGCTCTTAGCCCTTAATTACGTTTc                                                                                                                        >  1:258340/1‑121 (MQ=255)
                                                       aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGATGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCGTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGa                                                                                             >  4:124853/1‑147 (MQ=255)
                                                       aCCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGa                                                                                             >  7:11430/1‑147 (MQ=255)
                                                         cAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGc                                                                                         >  3:191889/1‑149 (MQ=255)
                                                          aaaaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                            <  7:360847/97‑1 (MQ=255)
                                                          aaaaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGctc                                                                                                                                            >  8:360847/1‑97 (MQ=255)
                                                           aaaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCg                                                                                        >  3:241894/1‑148 (MQ=255)
                                                            aaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGTGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGaa                                                                                            <  8:144036/143‑1 (MQ=255)
                                                            aaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGGGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGaa                                                                                            >  7:144036/1‑143 (MQ=255)
                                                            aaaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGa                                                                                      >  1:362370/1‑149 (MQ=255)
                                                             aaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGAt                                                                                     <  3:398990/149‑1 (MQ=255)
                                                             aaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGAt                                                                                     <  4:241894/149‑1 (MQ=255)
                                                             aaaGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGAt                                                                                     <  6:156416/149‑1 (MQ=255)
                                                               aGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCa                                                                                   <  8:146703/149‑1 (MQ=255)
                                                                   ttCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAAtt                                                                                                                               <  7:406012/101‑1 (MQ=255)
                                                                   ttCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAAtt                                                                                                                               >  8:406012/1‑101 (MQ=255)
                                                                   ttCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                               >  7:288701/1‑149 (MQ=255)
                                                                   ttCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTc                                                                               >  6:438267/1‑149 (MQ=255)
                                                                      gACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGTGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAg                                                                                                                                        <  3:70527/89‑1 (MQ=255)
                                                                      gACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAg                                                                                                                                        >  4:70527/1‑89 (MQ=255)
                                                                      gACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGAt                                                                                     >  5:51145/1‑140 (MQ=255)
                                                                      gACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGAt                                                                                     <  6:51145/140‑1 (MQ=255)
                                                                       aCAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAACTACGTTTCCGCTATCAGTTCa                                                                                                           <  2:180189/117‑1 (MQ=255)
                                                                       aCAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAACTACGTTTCCGCTATCAGTTCa                                                                                                           >  1:180189/1‑117 (MQ=255)
                                                                              cGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCaa                                                                    >  7:442531/1‑149 (MQ=255)
                                                                                            cAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAg                                                       >  7:346643/1‑148 (MQ=255)
                                                                                             aaCGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGaa                                                     >  5:445021/1‑149 (MQ=255)
                                                                                                      agaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGa                                                                                      <  1:392931/107‑1 (MQ=255)
                                                                                                      agaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGa                                                                                      >  2:392931/1‑107 (MQ=255)
                                                                                                      agaagaGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTg                                            >  3:154995/1‑149 (MQ=255)
                                                                                                         agagTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGTCCCTAGAACGATGATTGCtt                                         <  2:362370/149‑1 (MQ=255)
                                                                                                          gagTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCGTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCtt                                         >  3:261549/1‑148 (MQ=255)
                                                                                                           agTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAAc                                                               <  7:73655/125‑1 (MQ=255)
                                                                                                           agTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTCTGCTCATAGCCCTTAATTACGTTTACGCTATCAGATCAGAAACTGAAGCAGAAAGAGGATCAGTTCAAGCAGCGCAATTAAc                                                               >  8:73655/1‑125 (MQ=255)
                                                                                                                gTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTAt                                                                                                                  <  3:336775/69‑1 (MQ=255)
                                                                                                                gTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTAt                                                                                                                  >  4:336775/1‑69 (MQ=255)
                                                                                                                     aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAccc                             >  5:93347/1‑149 (MQ=255)
                                                                                                                     aaTGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAccc                             <  8:346643/149‑1 (MQ=255)
                                                                                                                       tGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                          >  6:286622/1‑134 (MQ=255)
                                                                                                                       tGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCt                                          <  5:286622/134‑1 (MQ=255)
                                                                                                                          gTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGttt                         >  8:449397/1‑148 (MQ=255)
                                                                                                                           ttAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTtc                       <  7:449397/149‑1 (MQ=255)
                                                                                                                            tAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCttt                                        <  7:253947/131‑1 (MQ=255)
                                                                                                                            tAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCttt                                        >  8:253947/1‑131 (MQ=255)
                                                                                                                               aTGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCa                   <  4:44154/149‑1 (MQ=255)
                                                                                                                                  ttAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATg                 <  6:445021/148‑1 (MQ=255)
                                                                                                                                   tAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGc                >  7:144697/1‑148 (MQ=255)
                                                                                                                                    taaGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAccc                             <  3:313435/133‑1 (MQ=39)
                                                                                                                                    taaGGGCTAAGAGCACACTACTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCAccc                             >  4:313435/2‑134 (MQ=39)
                                                                                                                                     aaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGAt             <  2:15141/149‑1 (MQ=255)
                                                                                                                                     aaGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCACCTAGAAAGATGATTGCTTTATAAATCACCCGTTTTCTCCATGCGAt             >  7:165384/1‑149 (MQ=255)
                                                                                                                                         gCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATAAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAg         >  6:107018/1‑149 (MQ=255)
                                                                                                                                          cTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATgagtg        <  5:438267/149‑5 (MQ=255)
                                                                                                                                          cTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGt        >  6:349629/1‑149 (MQ=255)
                                                                                                                                           tAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGt        >  7:79202/1‑148 (MQ=255)
                                                                                                                                           tAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGt        >  4:175343/1‑148 (MQ=255)
                                                                                                                                             agagTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTga      <  8:11430/148‑1 (MQ=255)
                                                                                                                                              gagTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCACTAGAACGATGATTGCTTTAAAAATAACCCGTTTTCTCCATGCGATGGAGTgag     >  1:419351/1‑148 (MQ=255)
                                                                                                                                               agtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGaa   >  3:399147/1‑149 (MQ=255)
                                                                                                                                                gtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt  <  4:191889/149‑1 (MQ=255)
                                                                                                                                                gtagtGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAt  <  8:79202/149‑1 (MQ=255)
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CTTTGGTATGGACGAAATGTTGCAGGGCTTCGCGGTGGCGCTGAAGATGGGGGCAACCAAAAAAGACTTCGACAATACCGTCGCCATTCACCCAACGGCGGCAGAAGAGTTCGTGACAATGCGTTAAATGTTAAAGGGCTAAGAGTAGTGTGCTCTTAGCCCTTAATTACGTTTCCGCTATCAGTTCAGAAGCTGAAGCAGAAAGCGGATCAGTTCCAGCAGCGCAATTAACGCCCCTAGAACGATGATTGCTTTATCAATCACCCGTTTTCTCCATGCGATGGAGTGAGAAT  >  NC_000913/3647525‑3647817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑