| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,168,349 | A→G | 8.4% | intergenic (+149/‑23) | rrsB → / → gltT | 16S ribosomal RNA of rrnB operon/tRNA‑Glu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,168,349 | 0 | A | G | 8.4% | 193.9 / 7.2 | 71 | intergenic (+149/‑23) | rrsB/gltT | 16S ribosomal RNA of rrnB operon/tRNA‑Glu |
| Reads supporting (aligned to +/- strand): ref base A (31/34); new base G (3/3); total (34/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTC > NC_000913/4168202‑4168495 | cttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCaa > 7:354391/1‑149 (MQ=39)cttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCaa > 6:416725/1‑149 (MQ=39) ttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAAc > 4:351825/1‑149 (MQ=39) ttaAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAAc > 2:465154/1‑149 (MQ=39) aaaGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgc < 3:72345‑M2/149‑3 (MQ=255) aaaGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACg > 6:7725‑M2/1‑147 (MQ=255) agaagCGTACTTTGTAGTGCTCACACAGATTGTTTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt < 3:234344‑M2/149‑5 (MQ=255) agaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAGGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt < 6:74627‑M2/149‑5 (MQ=255) agaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAGGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt > 5:74627‑M2/1‑145 (MQ=255) agaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCCACCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt < 7:412586‑M2/149‑5 (MQ=255) agaagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt < 4:175548‑M2/149‑5 (MQ=255) aagCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCGTTGCGTGa < 3:255357/149‑1 (MQ=255) gTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAg > 1:321382/1‑149 (MQ=39) gTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAg < 5:131271/149‑1 (MQ=39) gTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAg > 4:75757/1‑149 (MQ=39) aCTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGc > 5:20390/1‑148 (MQ=39) cTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCaa < 2:321382/149‑1 (MQ=39) gTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAAtttt > 5:25870/1‑149 (MQ=38) gTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAAttt < 2:5587/148‑1 (MQ=38) tAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTc < 4:388794/149‑1 (MQ=38) tAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTc < 8:206701/149‑1 (MQ=38) tCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcc > 6:263735/1‑149 (MQ=37) cacacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTccc > 1:175845/1‑149 (MQ=37) acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcccc > 6:42152/1‑149 (MQ=37) acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcccc > 1:292581/1‑149 (MQ=37) acacaGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcc < 6:217224/147‑1 (MQ=37) aGATTGTCTGATAGAAAGTGAGAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCg < 7:80556/149‑1 (MQ=37) gATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGt < 1:465154/149‑1 (MQ=37) aTTGTCTGATATAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTTAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACTCGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTc < 3:80370/149‑1 (MQ=255) tttGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga < 4:234344/147‑1 (MQ=37) tCTGATGAAAAGTGAATAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCGTTgcgagatattttcgtgtagggtgagctttccttaa < 2:14857‑M2/148‑36 (MQ=255) tCTGATGAAAAGTGAATAGCAAGGCGATTACGCGTTGGGAGTCAGGCTGAAGAGTATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATGACGCGTTgcgtgatattttcgtgtagggtgagctttcattaa > 1:14857‑M2/1‑113 (MQ=255) tCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTaga < 5:256833/149‑1 (MQ=37) tGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcc < 4:318810/135‑1 (MQ=37) tGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTcc > 3:318810/1‑135 (MQ=37) tGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAgg > 8:425397/1‑149 (MQ=37) gATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGc > 1:185276/1‑149 (MQ=35) aTAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTagag < 8:354391/146‑1 (MQ=35) tAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGcc > 4:318258/1‑148 (MQ=34) aGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt > 2:55316‑M2/1‑107 (MQ=255) aGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgt < 1:55316‑M2/111‑5 (MQ=255) gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCa < 6:270338/148‑1 (MQ=32) gAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAg < 5:416725/149‑1 (MQ=32) aaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGa < 3:351825/149‑1 (MQ=32) aaGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGa < 2:185276/149‑1 (MQ=32) tGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCaa > 3:327910/1‑100 (MQ=33) tGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCaa < 4:327910/100‑1 (MQ=33) gAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACAcc > 1:213992/1‑149 (MQ=32) aaaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACAcc < 6:25870/148‑1 (MQ=32) aaaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCg < 7:261431/149‑1 (MQ=32) aaaGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCTATTTTCGTGTCCCCTTCGTCTTGAGGCCCAGCACAcccc > 2:438817/1‑147 (MQ=9) agtcGTTTACGCGTTGGGAGTGAGGCTGAAGATAATAAGGCCGTTCGCTTTCTATTAATTAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTc < 5:96449/146‑1 (MQ=0) aCGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGATCACCCTACACGAAAATTGCTTATCACGCGTTgcgtgatattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctc > 6:415359‑M2/1‑89 (MQ=255) gcgTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgtgat < 2:49124‑M2/89‑8 (MQ=255) gcgTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgtgat > 1:49124‑M2/1‑82 (MQ=255) tGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACgcgtgata > 4:181171‑M2/1‑66 (MQ=255) gagaATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCGTTgcgtgatattttcgtgtagggtgagctttcattaa > 3:398115‑M2/1‑62 (MQ=255) gagaATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCGTTgcgtgatattttcgtgtagggtgagctttcattaa < 4:398115‑M2/97‑36 (MQ=255) ctcttcaTGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCACCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGttt < 8:412586/142‑1 (MQ=0) tACACGAAAAT‑‑‑‑‑ATCACGCAACgcgtgataagcaattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgctattcacttttcatcagacaatctgtgt < 1:434900‑M2/146‑126 (MQ=255) cacGAAAAT‑‑‑‑‑ATCACGCAACgcgtgataagcaattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgctattcacttttcatcagacaatctgtgtga > 2:434900‑M2/1‑19 (MQ=255) acGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTaa > 7:54138/1‑149 (MQ=32) gAAAAT‑‑‑‑‑ATCACGCAACgcgtgataagcaattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgcttttcactttc > 3:375386‑M2/1‑16 (MQ=255) gAAAAT‑‑‑‑‑ATCACGCAACgcgtgataagcaattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgcttttcactttc < 4:375386‑M2/124‑109 (MQ=255) gAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata > 5:375500/1‑149 (MQ=32) gAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata > 7:164199/1‑149 (MQ=32) gAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata > 6:357819/1‑149 (MQ=32) gAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAata > 5:399049/1‑149 (MQ=32) aaT‑‑‑‑‑ATCACGCAACgcgtgatattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgctattcacttttc > 6:245074‑M2/1‑13 (MQ=255) aaT‑‑‑‑‑ATCACGCAACgcgtgatattttcgtgtagggtgagctttcattaatagaaagcgaacggccttattctcttcagcctcactcccaacgcgtaaacgccttgctattcacttttc < 5:245074‑M2/117‑105 (MQ=255) at‑‑‑‑‑atCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATAtctc < 5:357819/149‑1 (MQ=32) gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACt > 4:365721/1‑148 (MQ=32) gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACt > 5:412010/1‑148 (MQ=32) gCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTc < 7:180116/149‑1 (MQ=33) gCAACGCGTGATAAGCAATTTTAGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTc < 3:318258/149‑1 (MQ=18) aaCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaaa > 5:187765/1‑144 (MQ=32) aaCGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCaaaa < 6:187765/144‑1 (MQ=32) | CTTAAAGAAGCGTACTTTGTAGTGCTCACACAGATTGTCTGATAGAAAGTGAAAAGCAAGGCGTTTACGCGTTGGGAGTGAGGCTGAAGAGAATAAGGCCGTTCGCTTTCTATTAATGAAAGCTCACCCTACACGAAAAT‑‑‑‑‑ATCACGCAACGCGTGATAAGCAATTTTCGTGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTTGCTGGTTTGTGAGTGAAAGTCGCCGACCTTAATATCTCAAAACTC > NC_000913/4168202‑4168495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |