Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,190,035 C→G 28.3% R84G (CGT→GGT)  yjaZ → stationary phase growth adaptation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,190,0350CG28.3% 215.3 / 101.0 126R84G (CGT→GGT) yjaZstationary phase growth adaptation protein
Reads supporting (aligned to +/- strand):  ref base C (45/44);  new base G (20/14);  total (66/60)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 131 total aligned reads displayed.

TCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGG  >  NC_000913/4189887‑4190182
                                                                                                                                                    |                                                                                                                                                   
tcatGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCAcc                                                                                                                                                     >  1:39605/1‑149 (MQ=255)
       ggCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTtata                                                                                                                                               >  5:389147/1‑148 (MQ=255)
       ggCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATaa                                                                                                                                              >  4:261264/1‑149 (MQ=255)
        gCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAAc                                                                                                                                             >  2:104583/1‑149 (MQ=255)
          aaaGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACt                                                                                                                                            >  5:405405/1‑148 (MQ=255)
          aaaGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACt                                                                                                                                            >  7:243908/1‑148 (MQ=255)
           aaGAGGCTTTAGATTATAGCTTTTGGGTTTCCTACTCTCTTCAGTGGTTCACTAAAGATTATGAACATCAGACGAACGAAGAATAACAATCGTTAATGTACCACGCGGCTAAAGAATCTCGTCCCTTCTTCCAGCACTGTTATAACTTa                                                                                                                                          <  2:148700/149‑1 (MQ=255)
              agCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAgcg                                                                                                                                       <  2:39605/149‑1 (MQ=255)
                ccTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAgcgcg                                                                                                                                     >  2:216521/1‑149 (MQ=255)
                  tttAGAGTATCGCTTTTGGGTTACCTAATCTATTCACTGCTTCACAAAAGATTATGTTCATCAGACGAACGAAGAAAAACAATCGTTAATGTACAACGCGCCTAAAGAATGTCGTCCCTTCTGCCAGCACGGTTATAACATCgcgcgct                                                                                                                                   >  2:140919/1‑148 (MQ=255)
                   ttAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGcac                                                                                                                                  >  4:378153/1‑149 (MQ=255)
                     agagTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGcaca                                                                                                                                 >  3:189023/1‑148 (MQ=255)
                     agagTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGcaca                                                                                                                                 >  5:279644/1‑148 (MQ=255)
                      gagTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGcacaca                                                                                                                               <  1:104583/149‑1 (MQ=255)
                             gCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTaaa                                                                                                                         <  3:383096/148‑1 (MQ=255)
                              cTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTaaaaa                                                                                                                       >  6:407809/1‑149 (MQ=255)
                               ttttGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCATAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTaaa                                                                                                                         >  1:291929/1‑146 (MQ=255)
                                 ttGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAg                                                                                                                      <  5:289425/147‑1 (MQ=255)
                                 ttGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTTTAACTTAGCGCGCACACACTTAAAAAGaa                                                                                                                    <  8:198790/148‑1 (MQ=255)
                                    ggTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTa                                                                                                                 <  1:58277/149‑1 (MQ=255)
                                     gTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGca                                                                                                                                   <  6:226808/130‑1 (MQ=255)
                                     gTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGca                                                                                                                                   >  5:226808/1‑130 (MQ=255)
                                      ttACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTAtt                                                                                                               <  7:148662/149‑1 (MQ=255)
                                        aCCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTAttt                                                                                                              <  1:216521/148‑1 (MQ=255)
                                         ccTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTg                                                                                                            <  6:405405/149‑1 (MQ=255)
                                         ccTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTg                                                                                                            <  5:407809/149‑1 (MQ=255)
                                         ccTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTATCGCGCACACACTTAAAAAGAACTAtttt                                                                                                             <  1:416797/148‑1 (MQ=255)
                                          cTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTgg                                                                                                           >  3:183006/1‑149 (MQ=255)
                                                tctTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     >  8:206577/1‑149 (MQ=255)
                                                tctTCACTGCTTCACAAAAGATTATGAACAACAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     >  2:62301/1‑149 (MQ=255)
                                                          ttCACAAAAGATTATGAAAATCAGACGAACGAAGAAAAACAATCGATAATGTACCACGAGCCTAAAGAATATCCTCCCTTCTGCCAGCACAGTTATAACTTAGCGCGCACACACTTaaaa                                                                                                                        >  7:20842/1‑120 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACTCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGATATAACTTAGCGCGCACACACTTAAAAAGAACTAAATTgg                                                                                                           >  8:245607/1‑128 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGca                                                                                                                                   <  7:280753/104‑1 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGca                                                                                                                                   >  8:280753/1‑104 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     <  5:123380/134‑1 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     >  6:123380/1‑134 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTa                                                                                      >  2:8568/1‑149 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTa                                                                                      <  8:243908/149‑1 (MQ=255)
                                                               aaaaGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCATAAAGAAACTCGTCCCATCTCCCAGCACCGTTATAACTTAGCGCGCACAAACATAAAAAGAACTATTTTGGCGCTCCCAGAAAGCAACGTTa                                                                                      >  5:8602/1‑149 (MQ=255)
                                                                         gAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCAGCCAGAAAGCAACGGTATTCATgaagg                                                                            >  3:356015/1‑145 (MQ=255)
                                                                          aaCATCAGAGGAACGAAGAAAAACAATCGTTAATGTAGCACGCGCCTAAAGATTCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            <  2:291929/148‑1 (MQ=255)
                                                                          aaCATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCggg                                                                           >  1:25085/1‑149 (MQ=255)
                                                                          aaCATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCggg                                                                           >  5:28780/1‑149 (MQ=255)
                                                                          aaCATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            <  6:279644/148‑1 (MQ=255)
                                                                            cATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTa                                                                         <  6:28780/149‑1 (MQ=255)
                                                                             aTCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            >  3:88366/1‑145 (MQ=255)
                                                                             aTCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTAt                                                                        >  2:229872/1‑149 (MQ=255)
                                                                              tCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATg                                                                       >  7:118481/1‑149 (MQ=255)
                                                                                aGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATgg                                                                      <  1:229872/148‑1 (MQ=255)
                                                                                 gACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATgg                                                                      >  8:19860/1‑147 (MQ=255)
                                                                                   cgaacgaaGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGc                                                                  <  2:167955/149‑1 (MQ=255)
                                                                                    gaacgaaGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTTTAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCTGTAAGCAACGTTATTCATGCGGGGTATgg                                                                      >  6:363160/1‑144 (MQ=255)
                                                                                    gaacgaaGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGc                                                                  >  4:431383/1‑148 (MQ=255)
                                                                                      acgaaGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCggg                                                                           <  5:349741/137‑1 (MQ=255)
                                                                                       cgaaGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATg                                                              >  7:72728/1‑149 (MQ=255)
                                                                                       cgaaGAAAAACAATCGTTAATGTACCACGCGACTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATg                                                              >  6:205512/1‑149 (MQ=255)
                                                                                        gaagaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGc                                                             <  8:118481/149‑1 (MQ=255)
                                                                                        gaagaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     >  8:49945/1‑109 (MQ=255)
                                                                                        gaagaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGc                                                                                                     <  7:49945/109‑1 (MQ=255)
                                                                                        gaagaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGc                                                             >  1:169204/1‑149 (MQ=255)
                                                                                         aagaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCa                                                                                                   >  6:241685/1‑110 (MQ=255)
                                                                                           gaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGt                                                          >  7:178459/1‑149 (MQ=255)
                                                                                           gaaAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGt                                                          <  3:431383/149‑1 (MQ=255)
                                                                                                 cAATCTTAAATTTACCACGCGCCTAAAGAATCTCTTACCTTATGCCAGCACCGTTATAACTTAGCTCGCACACACTTAAAAAGAACTATTTTTGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGa                                                    <  4:356015/149‑1 (MQ=255)
                                                                                                  aaTCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAg                                                   <  3:261264/149‑1 (MQ=255)
                                                                                                   aTCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAgg                                                  >  8:358357/1‑149 (MQ=255)
                                                                                                      gTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGCACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                >  5:134804/1‑148 (MQ=255)
                                                                                                      gTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTa                                                                    >  6:146981/1‑128 (MQ=255)
                                                                                                      gTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTa                                                                    <  5:146981/128‑1 (MQ=255)
                                                                                                      gTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTg                                                >  1:12518/1‑148 (MQ=255)
                                                                                                        tAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTAAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTAATGCCGCGTATGGTAGCTATGCAGTGAtt                                                      >  2:372713/1‑140 (MQ=255)
                                                                                                         aaTGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAATGAACTATTTTGGCGGTGCCAGAAAGCAACGTTATTCAAGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGAc                                             <  6:389147/148‑1 (MQ=255)
                                                                                                            gTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACt                                            >  6:213854/1‑146 (MQ=255)
                                                                                                              aCCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAg                                                                                                  <  1:469790/90‑1 (MQ=255)
                                                                                                              aCCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAg                                                                                                  >  2:469790/1‑90 (MQ=255)
                                                                                                               ccACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTAt                                                               >  4:249211/1‑124 (MQ=255)
                                                                                                                   gcgcCTAATGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagg                                  <  8:225821/149‑1 (MQ=255)
                                                                                                                   gcgcCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagg                                  <  3:307073/149‑1 (MQ=255)
                                                                                                                     gcCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATATCTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCATCGTTATTCATGCCGGGTATGGTAGCTATGCGGGGTGTGAGGTGGGGTTTGACGgaggag                                >  2:329793/1‑149 (MQ=255)
                                                                                                                       cTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGgaga                               >  7:118772/1‑148 (MQ=255)
                                                                                                                        tAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGCACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGAt                              >  1:116905/1‑148 (MQ=255)
                                                                                                                         aaaGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            >  7:438836/1‑101 (MQ=255)
                                                                                                                         aaaGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            <  8:445039/101‑1 (MQ=255)
                                                                                                                         aaaGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCgg                                                                            <  8:438836/101‑1 (MQ=255)
                                                                                                                          aaGAATCTCGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATaa                            <  1:80927/148‑1 (MQ=255)
                                                                                                                           aGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCTCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAgg                          <  5:230066/149‑1 (MQ=255)
                                                                                                                               tctcGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCAtt                      >  7:193059/1‑149 (MQ=255)
                                                                                                                                 tcGTCCCTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCAttt                     >  8:141913/1‑148 (MQ=255)
                                                                                                                                   gTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTAc                  <  5:205512/149‑1 (MQ=255)
                                                                                                                                     cccTTCTGCCAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGt                                                 <  2:205819/116‑1 (MQ=255)
                                                                                                                                     cccTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggagg                                  <  2:464453/131‑1 (MQ=255)
                                                                                                                                          cTGCCAGCCCGGTTATAACTTAGCGCGCACACACTTAAAAAGCACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGACGTGGACTTAGACGGAGGAGCTAAGGCATTTTCCTttgtt            <  1:140919/148‑1 (MQ=255)
                                                                                                                                          cTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTttgtt            <  1:8568/148‑1 (MQ=255)
                                                                                                                                             ccAGCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGt        >  1:181460/1‑149 (MQ=255)
                                                                                                                                               aGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGa                                                    >  4:218128/1‑103 (MQ=255)
                                                                                                                                                gCACGGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATTATGCATTTTACTTTGTTGCttt       >  4:227890/1‑147 (MQ=255)
                                                                                                                                                 cACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAg    >  1:337999/1‑149 (MQ=255)
                                                                                                                                                  aCCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgg   <  1:329793/149‑1 (MQ=255)
                                                                                                                                                  aCCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAgg   >  8:336715/1‑149 (MQ=255)
                                                                                                                                                    cGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACggag                                   >  1:204146/1‑115 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCATGAGGGCAAAGAGCCTTTAGAGTATCGCTTTTGGGTTACCTACTCTCTTCACTGCTTCACAAAAGATTATGAACATCAGACGAACGAAGAAAAACAATCGTTAATGTACCACGCGCCTAAAGAATCTCGTCCCTTCTGCCAGCACCGTTATAACTTAGCGCGCACACACTTAAAAAGAACTATTTTGGCGCTGCCAGAAAGCAACGTTATTCATGCCGGGTATGGTAGCTATGCCGTGATTGAGGTGGACTTAGACGGAGGAGATAAGGCATTTTACTTTGTTGCGTTCAGGG  >  NC_000913/4189887‑4190182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: