Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,257,743 T→C 5.3% M1T (ATG→ACG) † dinF → oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,257,7430TC 5.3% 416.7 / 9.2 132M1T (ATG→ACG) †dinFoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base T (59/66);  new base C (3/4);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.94e-01

Only 100 of 133 total aligned reads displayed.

CAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTC  >  NC_000913/4257595‑4257888
                                                                                                                                                    |                                                                                                                                                 
        tAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTg                                                                                                                                           >  8:449145/1‑149 (MQ=255)
          aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGc                                                                                                                                         >  6:337538/1‑149 (MQ=255)
          aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGc                                                                                                                                         <  8:446096/149‑1 (MQ=255)
                     ttGCCAGAAAATAGCGAGGTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTCTTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgtttg                                                                                                                              <  3:80110/149‑1 (MQ=255)
                       gCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTAGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgtttgtt                                                                                                                            >  4:228441/1‑149 (MQ=255)
                        ccAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgtttgttt                                                                                                                           <  5:337538/149‑1 (MQ=255)
                         cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCt                                                                                                                                            <  6:256611/131‑1 (MQ=255)
                         cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCt                                                                                                                                            >  5:256611/1‑131 (MQ=255)
                         cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGtttt                                                                                                                          >  8:176796/1‑149 (MQ=255)
                           gAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGttttt                                                                                                                         >  4:292213/1‑148 (MQ=255)
                             aaaTAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCa                                                                                                                       <  8:310808/148‑1 (MQ=255)
                                       tttAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcat                                                                                                            <  8:316848/149‑1 (MQ=255)
                                           aaCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                          <  5:384107/147‑1 (MQ=255)
                                            aCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtc                                                                                                              <  2:418662/142‑1 (MQ=255)
                                            aCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtc                                                                                                              >  1:418662/1‑142 (MQ=255)
                                              cAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCtt                                                                                                     <  7:16887/149‑1 (MQ=255)
                                                aTTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTg                                                                                                                                           >  3:59046/1‑109 (MQ=255)
                                                aTTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTg                                                                                                                                           <  4:59046/109‑1 (MQ=255)
                                                    tCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGc                                                                                               <  3:394194/149‑1 (MQ=255)
                                                      gTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCAt                                                                                             >  2:296927/1‑149 (MQ=255)
                                                       ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCa                                                                                                                       <  3:23321/122‑1 (MQ=255)
                                                       ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCa                                                                                                                       >  4:23321/1‑122 (MQ=255)
                                                       ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATg                                                                                            >  7:12020/1‑149 (MQ=255)
                                                       ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATg                                                                                            <  1:296927/149‑1 (MQ=255)
                                                       ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATg                                                                                            >  8:426753/1‑149 (MQ=255)
                                                         gACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAAACCGCGACGCCAGGCGGCATCGCGgtct                                                                                                                               <  5:10793/112‑3 (MQ=38)
                                                         gACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAAACCGCGACGCCAGGCGGCATCGCGgtct                                                                                                                               >  6:10793/1‑110 (MQ=38)
                                                              tCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCa                                                                                         >  7:380239/1‑145 (MQ=255)
                                                              tCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCa                                                                                         <  8:380239/145‑1 (MQ=255)
                                                               cGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTATCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTc                                                                                      <  6:448645/147‑1 (MQ=255)
                                                                gTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCt                                                                                    <  3:68151/148‑1 (MQ=255)
                                                                gTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCt                                                                                    <  2:304685/148‑1 (MQ=255)
                                                                gTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCt                                                                                    >  5:5784/1‑148 (MQ=255)
                                                                gTCAGCAGAGCTTCACCATTGAAGGGCTGGGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                          >  3:283160/1‑125 (MQ=255)
                                                                    gcagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACtt                                                                               >  6:225227/1‑149 (MQ=255)
                                                                    gcagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACtt                                                                               <  3:84283/149‑1 (MQ=255)
                                                                     cagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgt                                                                                                                                 >  7:324310/1‑98 (MQ=255)
                                                                     cagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgt                                                                                                                                 <  8:324310/98‑1 (MQ=255)
                                                                       gagCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCtt                                                                                                     <  7:260447/124‑1 (MQ=255)
                                                                       gagCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCtt                                                                                                     >  8:260447/1‑124 (MQ=255)
                                                                        agCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATc                                                                           <  3:63211/149‑1 (MQ=255)
                                                                          cTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTTTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTc                                                                              >  4:217091/1‑144 (MQ=255)
                                                                          cTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTc                                                                              <  3:217091/144‑1 (MQ=255)
                                                                             cACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATGTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGAt                                                                       >  5:189852/1‑148 (MQ=255)
                                                                             cACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGAt                                                                       >  6:260526/1‑148 (MQ=255)
                                                                              aCCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATa                                                                      <  4:397517/148‑1 (MQ=255)
                                                                               ccATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                    <  3:228441/149‑1 (MQ=255)
                                                                               ccATTGAAGGGCAGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATa                                                                      >  6:334405/1‑147 (MQ=255)
                                                                                         gCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGc                                                          <  5:20712/149‑1 (MQ=255)
                                                                                           gggCGGTGGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCa                                                                                  <  6:342303/122‑1 (MQ=255)
                                                                                                 ttGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGcc                                                  >  1:273493/1‑149 (MQ=255)
                                                                                                       ttATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTAcc                                             >  5:400044/1‑148 (MQ=255)
                                                                                                            cGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGa                                        <  3:292213/148‑1 (MQ=255)
                                                                                                                  ggCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtctc                                 <  7:444432/149‑1 (MQ=255)
                                                                                                                  ggCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtctc                                 <  7:449145/149‑1 (MQ=255)
                                                                                                                  ggCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtctc                                 <  8:12020/149‑1 (MQ=255)
                                                                                                                   gcctgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                    >  7:202706‑M1/27‑113 (MQ=255)
                                                                                                                   gcctgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                    <  8:202706‑M1/87‑1 (MQ=255)
                                                                                                                   gCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTcc                                <  5:260526/149‑1 (MQ=255)
                                                                                                                     gACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCaa                              <  6:225570/149‑1 (MQ=255)
                                                                                                                     gACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCaa                              <  7:255574/149‑1 (MQ=255)
                                                                                                                     gACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCaa                              <  4:314234/149‑1 (MQ=255)
                                                                                                                      tgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtct                                  >  3:34163‑M1/24‑144 (MQ=255)
                                                                                                                          gcggTAACATATCTCTGAGACAGCGATGCCGCCTGGAGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTGAATCTGATAAATACATATGGCATCTCGCCTTAGCCATGATTTTCTCCAATATcc                         >  5:310096/4‑148 (MQ=255)
                                                                                                                          gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCt                                                                          <  2:174919/100‑1 (MQ=255)
                                                                                                                          gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCt                                                                          >  1:174919/1‑100 (MQ=255)
                                                                                                                          gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAatat                           <  6:5784/147‑1 (MQ=255)
                                                                                                                           cTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCAt                                          <  1:131005/131‑1 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCtt                                                                                                               >  1:163353/1‑61 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCtt                                                                                                               <  2:163353/61‑1 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCt                                                                                                 >  3:63478/1‑75 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCt                                                                                                 <  4:63478/75‑1 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                    >  8:437812/1‑104 (MQ=255)
                                                                                                                            tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                    <  7:437812/104‑1 (MQ=255)
                                                                                                                                aCATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAAAATAAccgtta                   >  4:304674/1‑148 (MQ=255)
                                                                                                                                 gatgaaaaacaaACCGCGACGCCAGGCGGCATCGCTGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCGTCACTTCATCTGATAAAGCAATCTGGCATCTCGCCTTACACATGATTTTCTCCATTATcaccgttgc                  <  1:338503‑M1/137‑3 (MQ=255)
                                                                                                                                 cATATCTCTTAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttcc                  <  3:304674/149‑1 (MQ=255)
                                                                                                                                 cATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGa                                        <  6:106862/127‑1 (MQ=255)
                                                                                                                                 cATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGa                                        >  5:106862/1‑127 (MQ=255)
                                                                                                                                 cATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttcc                  <  2:273493/149‑1 (MQ=255)
                                                                                                                                  atatCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccg                 >  2:359561/1‑149 (MQ=255)
                                                                                                                                  atatCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGCCATTCCTCACTTCAGCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccg                 >  5:120879/1‑149 (MQ=255)
                                                                                                                                    atCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccgtt               >  6:267704/1‑149 (MQ=255)
                                                                                                                                        ctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAat                             >  5:449744/1‑131 (MQ=255)
                                                                                                                                        ctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTg           >  3:335608/1‑149 (MQ=255)
                                                                                                                                         tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg              <  8:275045/145‑1 (MQ=255)
                                                                                                                                         tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg              <  8:282045/145‑1 (MQ=255)
                                                                                                                                         tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg              >  7:275045/1‑145 (MQ=255)
                                                                                                                                         tGAGACCGCGATGCCGCATGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg          >  8:472886/1‑149 (MQ=255)
                                                                                                                                           agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTGCGTTGCTgg          <  6:189852/147‑1 (MQ=255)
                                                                                                                                           agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg          <  3:139237/147‑1 (MQ=255)
                                                                                                                                           agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg          <  6:400044/147‑1 (MQ=255)
                                                                                                                                           agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTg           <  5:225227/146‑1 (MQ=255)
                                                                                                                                           agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGa        >  2:419514/1‑149 (MQ=255)
                                                                                                                                            gaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGa        <  6:443986/148‑1 (MQ=255)
                                                                                                                                                 CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTa                                               >  5:76981‑M1/1‑104 (MQ=255)
                                                                                                                                                 CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTa                                               <  6:76981‑M1/104‑1 (MQ=255)
                                                                                                                                                 CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTg     <  5:455216‑M1/146‑1 (MQ=255)
                                                                                                                                                 CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTg     >  6:455216‑M1/1‑146 (MQ=255)
                                                                                                                                                   ATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTAc                                              >  7:295761‑M1/1‑103 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CAGGGCAATAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTC  >  NC_000913/4257595‑4257888

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: