Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,257,747 G→A 5.2% P2P (CCG→CCA dinF → oxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,257,7470GA 5.2% 426.5 / 7.1 132P2P (CCG→CCAdinFoxidative stress resistance protein; putative MATE family efflux pump; UV and mitomycin C inducible protein
Reads supporting (aligned to +/- strand):  ref base G (59/66);  new base A (3/4);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

TAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGG  >  NC_000913/4257603‑4257895
                                                                                                                                                |                                                                                                                                                     
tAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTg                                                                                                                                                   >  8:449145/1‑149 (MQ=255)
  aaGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGc                                                                                                                                                 >  6:337538/1‑149 (MQ=255)
                 cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCt                                                                                                                                                    >  5:256611/1‑131 (MQ=255)
                 cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCt                                                                                                                                                    <  6:256611/131‑1 (MQ=255)
                 cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGtttt                                                                                                                                  >  8:176796/1‑149 (MQ=255)
                 cAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGtttt                                                                                                                                  >  3:143182/1‑149 (MQ=255)
                   gAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGttttt                                                                                                                                 >  4:292213/1‑148 (MQ=255)
                             aGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCtt                                                                                                                      >  1:284867/1‑148 (MQ=255)
                             aGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGGTTTTCATCTCTCtt                                                                                                                      >  3:63765/1‑148 (MQ=255)
                               tttAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcat                                                                                                                   <  8:316848/149‑1 (MQ=255)
                                  aaaCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGCTGATTCGCAACGGCGACTGGCTGTAACATATCTCAGAGACCGCGATTCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAg                                                                                                                >  6:330030/1‑149 (MQ=255)
                                   aaCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                                 <  5:384107/147‑1 (MQ=255)
                                   aaCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                                 >  6:384107/1‑147 (MQ=255)
                                    aCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtc                                                                                                                     <  2:418662/142‑1 (MQ=255)
                                    aCCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGc                                                                                                              <  4:143182/149‑1 (MQ=255)
                                    aCCAATTGTCGTTGACCTTCGTCAGCAGAGCTACACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGc                                                                                                              >  6:234938/1‑149 (MQ=255)
                                      cAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCtt                                                                                                            <  7:16887/149‑1 (MQ=255)
                                        aTTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTg                                                                                                                                                   >  3:59046/1‑109 (MQ=255)
                                           gTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCt                                                                                                        <  6:98833/148‑1 (MQ=255)
                                            tCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGc                                                                                                      <  3:394194/149‑1 (MQ=255)
                                              gTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCAt                                                                                                    <  5:234938/149‑1 (MQ=255)
                                              gTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCAt                                                                                                    >  2:296927/1‑149 (MQ=255)
                                              gTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCAt                                                                                                    >  3:397517/1‑149 (MQ=255)
                                               ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCa                                                                                                                              >  4:23321/1‑122 (MQ=255)
                                               ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCa                                                                                                                              <  3:23321/122‑1 (MQ=255)
                                               ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATg                                                                                                   >  8:426753/1‑149 (MQ=255)
                                               ttGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATg                                                                                                   <  1:296927/149‑1 (MQ=255)
                                                tGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGc                                                                                                      <  7:71887/145‑1 (MQ=255)
                                                 gACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAAACCGCGACGCCAGGCGGCATCGCGgtct                                                                                                                                       >  6:10793/1‑110 (MQ=38)
                                                    cTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCAtt                                                                                              >  8:255574/1‑149 (MQ=255)
                                                    cTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCAtt                                                                                              >  4:68151/1‑149 (MQ=255)
                                                      tCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCa                                                                                                >  7:380239/1‑145 (MQ=255)
                                                       cGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTATCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTc                                                                                             <  6:448645/147‑1 (MQ=255)
                                                        gTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCt                                                                                           <  3:68151/148‑1 (MQ=255)
                                                        gTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCt                                                                                           >  5:5784/1‑148 (MQ=255)
                                                        gTCAGCAGAGCTTCACCATTGAAGGGCTGGGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                                 >  3:283160/1‑125 (MQ=255)
                                                        gTCAGCAGAGCTTCACCATTGAAGGGCTGGGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTtcatca                                                                                                                 <  4:283160/125‑1 (MQ=255)
                                                            gcagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACtt                                                                                      <  3:84283/149‑1 (MQ=255)
                                                             cagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgt                                                                                                                                         >  7:324310/1‑98 (MQ=255)
                                                             cagAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGgt                                                                                                                                         <  8:324310/98‑1 (MQ=255)
                                                               gagCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCtt                                                                                                            <  7:260447/124‑1 (MQ=255)
                                                                  cTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTTTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTc                                                                                     >  4:217091/1‑144 (MQ=255)
                                                                  cTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTc                                                                                     <  3:217091/144‑1 (MQ=255)
                                                                     cACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATGTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGAt                                                                              >  5:189852/1‑148 (MQ=255)
                                                                      aCCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATa                                                                             <  4:397517/148‑1 (MQ=255)
                                                                          ttGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCa                                                                        >  8:444432/1‑149 (MQ=255)
                                                                            gAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCAct                                                                      >  5:166834/1‑149 (MQ=255)
                                                                                   gggCGGTGGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCa                                                                                         <  6:342303/122‑1 (MQ=255)
                                                                                     gCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCAtct                                                             <  7:426753/149‑1 (MQ=255)
                                                                                               ttATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTAcc                                                    >  5:400044/1‑148 (MQ=255)
                                                                                                 aTTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCAt                                                 >  3:314234/1‑149 (MQ=255)
                                                                                                     gCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGAtt                                             <  2:284867/149‑1 (MQ=255)
                                                                                                          ggCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtctc                                        <  7:444432/149‑1 (MQ=255)
                                                                                                          ggCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtctc                                        <  7:449145/149‑1 (MQ=255)
                                                                                                           gcctgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                           >  7:202706‑M1/27‑113 (MQ=255)
                                                                                                           gcctgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                           <  8:202706‑M1/87‑1 (MQ=255)
                                                                                                             gACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCaa                                     <  4:314234/149‑1 (MQ=255)
                                                                                                             gACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCaa                                     <  6:225570/149‑1 (MQ=255)
                                                                                                              tgatgaagagagatgaaaaacaaACCGCGACGCCAGGCGGCATCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTtct                                         <  4:34163‑M1/121‑1 (MQ=255)
                                                                                                                  gcggTAACATATCTCTGAGACAGCGATGCCGCCTGGAGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTGAATCTGATAAATACATATGGCATCTCGCCTTAGCCATGATTTTCTCCAATATcc                                >  5:310096/4‑148 (MQ=255)
                                                                                                                  gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCt                                                                                 <  2:174919/100‑1 (MQ=255)
                                                                                                                  gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCt                                                                                 >  1:174919/1‑100 (MQ=255)
                                                                                                                  gCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAatat                                  <  6:5784/147‑1 (MQ=255)
                                                                                                                   cTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCAt                                                 >  2:131005/1‑131 (MQ=255)
                                                                                                                   cTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCAt                                                 <  1:131005/131‑1 (MQ=255)
                                                                                                                    tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCtt                                                                                                                      >  1:163353/1‑61 (MQ=255)
                                                                                                                    tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCtt                                                                                                                      <  2:163353/61‑1 (MQ=255)
                                                                                                                    tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCt                                                                                                        >  3:63478/1‑75 (MQ=255)
                                                                                                                    tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCt                                                                                                        <  4:63478/75‑1 (MQ=255)
                                                                                                                    tGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATaaa                                                                           >  8:437812/1‑104 (MQ=255)
                                                                                                                         gatgaaaaacaaACCGCGACGCCAGGCGGCATCGCTGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCGTCACTTCATCTGATAAAGCAATCTGGCATCTCGCCTTACACATGATTTTCTCCATTATcaccgttgc                         <  1:338503‑M1/137‑3 (MQ=255)
                                                                                                                         cATATCTCTTAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttcc                         <  3:304674/149‑1 (MQ=255)
                                                                                                                         cATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGa                                               >  5:106862/1‑127 (MQ=255)
                                                                                                                         cATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttcc                         <  2:273493/149‑1 (MQ=255)
                                                                                                                          atatCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccg                        >  2:359561/1‑149 (MQ=255)
                                                                                                                          atatCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGCCATTCCTCACTTCAGCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccg                        >  5:120879/1‑149 (MQ=255)
                                                                                                                            atCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCAccgttccgtt                      >  6:267704/1‑149 (MQ=255)
                                                                                                                                ctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAat                                    <  6:449744/131‑1 (MQ=255)
                                                                                                                                ctGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTg                  >  3:335608/1‑149 (MQ=255)
                                                                                                                                 tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg                     >  7:275045/1‑145 (MQ=255)
                                                                                                                                 tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg                     >  7:282045/1‑145 (MQ=255)
                                                                                                                                 tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg                     <  8:282045/145‑1 (MQ=255)
                                                                                                                                 tGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTg                     <  8:275045/145‑1 (MQ=255)
                                                                                                                                 tGAGACCGCGATGCCGCATGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg                 >  8:472886/1‑149 (MQ=255)
                                                                                                                                  gagaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg                 >  3:271980/1‑148 (MQ=255)
                                                                                                                                   agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCCTGATTTTCTCCAATATCACCGTTCCGTTGCTgg                 <  6:166834/147‑1 (MQ=255)
                                                                                                                                   agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTGCGTTGCTgg                 <  6:189852/147‑1 (MQ=255)
                                                                                                                                   agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg                 <  3:139237/147‑1 (MQ=255)
                                                                                                                                   agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTg                  <  5:225227/146‑1 (MQ=255)
                                                                                                                                   agaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGa               <  5:334405/149‑1 (MQ=255)
                                                                                                                                    gaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGa               >  5:443986/1‑148 (MQ=255)
                                                                                                                                    gaCCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGa               <  6:443986/148‑1 (MQ=255)
                                                                                                                                       CGCGATGCCGCCTGGCATCGCGGTTTGTTTTTTCATCACTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTg            <  2:374223‑M1/149‑1 (MQ=255)
                                                                                                                                         CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTa                                                      >  5:76981‑M1/1‑104 (MQ=255)
                                                                                                                                         CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTa                                                      <  6:76981‑M1/104‑1 (MQ=255)
                                                                                                                                         CGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTg            <  5:455216‑M1/146‑1 (MQ=255)
                                                                                                                                          GATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTgg                 <  7:207999‑M1/140‑1 (MQ=255)
                                                                                                                                          GATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTc         <  8:327180‑M1/148‑1 (MQ=255)
                                                                                                                                           ATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTAc                                                     <  8:295761‑M1/103‑1 (MQ=255)
                                                                                                                                                gccGGGCGGCGCGGGTTGTTTTTAATCTCTCTTCAGCATGCTTGTCTGCATGGCATTCCTCACTGCATCACCGAAAGCACCCGCGCATCTCCCCTTACCCCTGATTTTCTCCAAAATCACCGTGACGTTGCTGGGACTGGTCGATAcgg  <  6:310096/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
TAAAGTCGAACTGTTGCCAGAAAATAGCGAGTTTAAACCAATTGTCGTTGACCTTCGTCAGCAGAGCTTCACCATTGAAGGGCTGGCGGTTGGGGTTATTCGCAACGGCGACTGGCTGTAACATATCTCTGAGACCGCGATGCCGCCTGGCGTCGCGGTTTGTTTTTCATCTCTCTTCATCAGGCTTGTCTGCATGGCATTCCTCACTTCATCTGATAAAGCACTCTGGCATCTCGCCTTACCCATGATTTTCTCCAATATCACCGTTCCGTTGCTGGGACTGGTCGATACGG  >  NC_000913/4257603‑4257895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: