Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 641,360 C→T 5.6% intergenic (+42/+79) ahpF → / ← uspG alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913641,3600CT 5.6% 465.5 / 10.3 143intergenic (+42/+79)ahpF/uspGalkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12
Reads supporting (aligned to +/- strand):  ref base C (70/65);  new base T (5/3);  total (75/68)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

Only 100 of 143 total aligned reads displayed.

TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATG  >  NC_000913/641217‑641507
                                                                                                                                               |                                                                                                                                                   
  tACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTg                                                                                                                                              >  7:349060/1‑149 (MQ=255)
  tACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTg                                                                                                                                              >  4:63468/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                  >  6:253395/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                  <  1:193663/149‑1 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                  >  5:216675/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                  <  8:158689/149‑1 (MQ=255)
               tACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTAt                                                                                                                                 >  3:282103/1‑149 (MQ=255)
                 cAATCATATCATCATCGCAAATGGCGAAGGTTCCAAAGCATATCTTATTGCTTTTGACTACCTTATTCGCACCAACACTGCATAAGAAGAAGTAAGATTAAACTGCAATTGCTTAGCCGACGGTGTCAAACCTGGCGGCTTTTTTATgg                                                                                                                               <  5:31789/149‑1 (MQ=255)
                 cAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATg                                                                                                                                <  4:324010/148‑1 (MQ=255)
                    gCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAt                                                                                                                            <  6:216675/149‑1 (MQ=255)
                     cAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                            <  1:286906/132‑1 (MQ=255)
                     cAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                            >  2:286906/1‑132 (MQ=255)
                         tcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTaaaa                                                                                                                       <  6:315090/149‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                        <  5:387273/127‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                        >  6:387273/1‑127 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGccc                                                                                                                  >  7:51923/1‑149 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGccc                                                                                                                  >  1:374362/1‑149 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                 >  8:355714/1‑149 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                 >  2:251762/1‑149 (MQ=255)
                                  ccACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgcc                                                                                                              <  6:404161/149‑1 (MQ=255)
                                   cACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGACGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgccg                                                                                                             <  1:169965/149‑1 (MQ=255)
                                   cACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgccg                                                                                                             >  7:179188/1‑149 (MQ=255)
                                        gCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAt                                                                                                         <  3:70510/148‑1 (MQ=255)
                                               tGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCgg                                                                                                 <  4:296311/149‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                            <  5:265856/102‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCa                                                                                                                             <  7:341104/117‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCa                                                                                                                             >  8:341104/1‑117 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                             >  4:146160/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                             <  5:314055/149‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                             >  1:106578/1‑149 (MQ=255)
                                                    aaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAgg                                                                                            <  8:332710/149‑1 (MQ=255)
                                                     aGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAggg                                                                                           <  7:420640/149‑1 (MQ=255)
                                                       ccTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCg                                                                                         >  3:336491/1‑149 (MQ=255)
                                                        ctctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCgg                                                                                        <  2:374362/149‑1 (MQ=255)
                                                          ctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                      >  4:147259/1‑149 (MQ=255)
                                                               aGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                   <  5:161440/147‑1 (MQ=255)
                                                               aGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATa                                                                                    <  1:241363/146‑1 (MQ=255)
                                                                 tGCTTTTGACTACCTGATTCGCACCAAAAATGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTAt                                                                               >  4:316069/1‑149 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                      >  5:448216/1‑139 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCt                                                                            >  2:390800/1‑149 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                          >  5:201390/1‑144 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                          <  6:201390/144‑1 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                   <  4:336491/149‑1 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                   >  4:220416/1‑149 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                   >  1:447631/1‑149 (MQ=255)
                                                                              cTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAAc                                                                  >  8:383924/1‑149 (MQ=255)
                                                                              cTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAAc                                                                  <  1:279396/149‑1 (MQ=255)
                                                                                gATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                     >  2:19621/1‑96 (MQ=255)
                                                                                gATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACg                                                                 >  2:254648/1‑148 (MQ=255)
                                                                                 aTTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACg                                                                 <  4:22671/147‑1 (MQ=255)
                                                                                     gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                           <  6:452518/149‑1 (MQ=255)
                                                                                     gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                           >  1:86935/1‑149 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAATGCACAAc                                                           >  4:244067/1‑148 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAcc                                                          <  3:428952/149‑1 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGAAATTAACGCACAAc                                                           >  4:90683/1‑148 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAACCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                           >  8:414754/1‑148 (MQ=255)
                                                                                       aCCAAAACTGCGTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAGAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                           >  3:332042/1‑147 (MQ=255)
                                                                                       aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                           >  6:258352/1‑147 (MQ=255)
                                                                                        ccAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAg                                                        <  2:447631/149‑1 (MQ=255)
                                                                                           aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAc                                                     <  8:51923/149‑1 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                  >  2:448860/1‑148 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                  >  2:7904/1‑148 (MQ=255)
                                                                                                  aTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                  >  1:442016/1‑145 (MQ=255)
                                                                                                   tAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGAt                                             >  6:341418/1‑149 (MQ=255)
                                                                                                     agaagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGAtt                                            <  1:254648/148‑1 (MQ=255)
                                                                                                       aagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggc                                         <  3:98714/149‑1 (MQ=255)
                                                                                                          aagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtg                                      <  7:414754/149‑1 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                    >  3:198258/1‑149 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                    <  1:7904/149‑1 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACaa                                                            >  2:283934/1‑124 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACaa                                                            <  1:283934/124‑1 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggcg                                   >  2:71406/1‑149 (MQ=255)
                                                                                                                gATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                   >  3:356176/1‑98 (MQ=255)
                                                                                                                gATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                   <  4:356176/98‑1 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGAt                                >  6:68069/1‑148 (MQ=255)
                                                                                                                  ttCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCa                              <  2:302883/149‑1 (MQ=255)
                                                                                                                     aCCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                           >  7:170757/1‑149 (MQ=255)
                                                                                                                     aCCTGCAATAGATTAGCCGCTGGGGTCAAACATGCCGGCTTTTTTATCGCAATAAAAAGCCCCGCCGGGATGGCTCCCGGCAGGGCGGAATAATTAATCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGTCGGATAACGc                           >  8:313007/1‑149 (MQ=255)
                                                                                                                      ccTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCg                                                   <  3:226365/124‑1 (MQ=255)
                                                                                                                            ataaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                    >  7:90414/8‑149 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                               >  8:50095/1‑138 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                    <  1:71406/149‑1 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGTGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                   >  4:78896/1‑149 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                   <  8:170757/149‑1 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                   >  4:51264/1‑149 (MQ=255)
                                                                                                                             taaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                   >  1:365636/7‑149 (MQ=255)
                                                                                                                               aaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtgg                                     >  3:10381/5‑129 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                  >  7:393734/1‑147 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                <  5:68069/149‑1 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                <  3:316729/149‑1 (MQ=255)
                                                                                                                                aaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                  >  2:127454/4‑147 (MQ=255)
                                                                                                                                 ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                  >  1:223627/1‑146 (MQ=255)
                                                                                                                                 ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAAGGCTCCGGGCAGGGCGGGATACTTAATCTGGCAATTAACGCAAAACCATCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                  >  8:421354/1‑146 (MQ=255)
                                                                                                                                       gccgGGGTCAGACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                           >  5:334297/1‑131 (MQ=255)
                                                                                                                                       gccgGGGTCACACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                           <  6:334297/131‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa        <  7:439337/149‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa        <  3:147259/149‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                               <  7:153358/120‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATg  <  7:355714/149‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATg  <  1:251762/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATG  >  NC_000913/641217‑641507

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: