Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 641,361 A→G 5.6% intergenic (+43/+78) ahpF → / ← uspG alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913641,3610AG 5.6% 453.4 / 11.1 143intergenic (+43/+78)ahpF/uspGalkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12
Reads supporting (aligned to +/- strand):  ref base A (70/65);  new base G (5/3);  total (75/68)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 148 total aligned reads displayed.

TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGG  >  NC_000913/641217‑641509
                                                                                                                                                |                                                                                                                                                    
tGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAAcc                                                                                                                                                  >  5:315090/1‑149 (MQ=255)
           tCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCtttt                                                                                                                                       >  4:70510/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                    <  1:193663/149‑1 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                    >  5:216675/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                    >  6:253395/1‑149 (MQ=255)
               tACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTAt                                                                                                                                   >  3:282103/1‑149 (MQ=255)
                 cAATCATATCATCATCGCAAATGGCGAAGGTTCCAAAGCATATCTTATTGCTTTTGACTACCTTATTCGCACCAACACTGCATAAGAAGAAGTAAGATTAAACTGCAATTGCTTAGCCGACGGTGTCAAACCTGGCGGCTTTTTTATgg                                                                                                                                 <  5:31789/149‑1 (MQ=255)
                 cAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATg                                                                                                                                  <  4:324010/148‑1 (MQ=255)
                    gCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAt                                                                                                                              <  6:216675/149‑1 (MQ=255)
                     cAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                              >  2:286906/1‑132 (MQ=255)
                       tatcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTa                                                                                                                            >  7:332710/2‑148 (MQ=255)
                        atcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTaa                                                                                                                           >  2:310424/1‑148 (MQ=255)
                         tcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTaaaa                                                                                                                         <  6:315090/149‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                          <  5:387273/127‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                          >  6:387273/1‑127 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                   >  8:355714/1‑149 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                   >  2:251762/1‑149 (MQ=255)
                                   cACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGACGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgccg                                                                                                               <  1:169965/149‑1 (MQ=255)
                                        gCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAt                                                                                                           <  3:70510/148‑1 (MQ=255)
                                          gAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGc                                                                                                        >  7:35073/1‑149 (MQ=255)
                                               tGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCgg                                                                                                   <  4:296311/149‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                              <  5:265856/102‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCa                                                                                                                               <  7:341104/117‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCa                                                                                                                               >  8:341104/1‑117 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                               >  1:106578/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                               >  4:428952/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                               >  4:146160/1‑149 (MQ=255)
                                                   aaaGCCGCTCTGAGTGCCTTTGACTACCTGATTCCCACCAAAACTGCATAAGAAGAACTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGTCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCGCCGGTCAg                                                                                               <  6:367143/149‑1 (MQ=255)
                                                    aaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAgg                                                                                              <  8:332710/149‑1 (MQ=255)
                                                     aGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAggg                                                                                             >  1:302883/1‑149 (MQ=255)
                                                       ccTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCg                                                                                           >  3:336491/1‑149 (MQ=255)
                                                        ctctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCgg                                                                                          <  8:179188/149‑1 (MQ=255)
                                                        ctctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCgg                                                                                          <  2:374362/149‑1 (MQ=255)
                                                          ctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                        >  4:147259/1‑149 (MQ=255)
                                                               aGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATa                                                                                      <  1:241363/146‑1 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                        <  6:448216/139‑1 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCt                                                                              >  4:98714/1‑149 (MQ=255)
                                                                        gACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGc                                                                           <  4:282103/148‑1 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                            <  6:201390/144‑1 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                            >  5:201390/1‑144 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                     >  1:447631/1‑149 (MQ=255)
                                                                              cTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAAc                                                                    >  8:383924/1‑149 (MQ=255)
                                                                              cTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAAc                                                                    <  1:279396/149‑1 (MQ=255)
                                                                                gATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                       >  2:19621/1‑96 (MQ=255)
                                                                                gATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACg                                                                   >  2:254648/1‑148 (MQ=255)
                                                                                 aTTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACg                                                                   <  4:22671/147‑1 (MQ=255)
                                                                                     gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             >  1:86935/1‑149 (MQ=255)
                                                                                     gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             <  6:452518/149‑1 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAcc                                                            <  3:428952/149‑1 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAACCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             >  8:414754/1‑148 (MQ=255)
                                                                                       aCCAAAACTGCGTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAGAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             >  3:332042/1‑147 (MQ=255)
                                                                                       aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             >  4:316729/1‑147 (MQ=255)
                                                                                       aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                             >  6:258352/1‑147 (MQ=255)
                                                                                        ccAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAg                                                          <  2:447631/149‑1 (MQ=255)
                                                                                           aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAc                                                       <  8:51923/149‑1 (MQ=255)
                                                                                           aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAc                                                       >  1:401255/1‑149 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                    >  2:448860/1‑148 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                    >  2:7904/1‑148 (MQ=255)
                                                                                                 cATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAg                                                 <  3:316069/149‑1 (MQ=255)
                                                                                                     agaagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGAtt                                              <  1:254648/148‑1 (MQ=255)
                                                                                                       aagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggc                                           <  3:98714/149‑1 (MQ=255)
                                                                                                          aagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtg                                        <  7:414754/149‑1 (MQ=255)
                                                                                                           agTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtg                                        <  2:442016/148‑1 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                      <  1:7904/149‑1 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                      >  3:198258/1‑149 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACaa                                                              >  2:283934/1‑124 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACaa                                                              <  1:283934/124‑1 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggcg                                     >  2:71406/1‑149 (MQ=255)
                                                                                                                gATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                     <  4:356176/98‑1 (MQ=255)
                                                                                                                gATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                     >  3:356176/1‑98 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGAt                                  >  6:68069/1‑148 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 <  3:220416/149‑1 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 <  1:464369/149‑1 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCACCACCGGCAGATTGGCGTGGCGGATc                                 <  5:341418/149‑1 (MQ=255)
                                                                                                                     aCCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                             >  7:170757/1‑149 (MQ=255)
                                                                                                                      ccTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCg                                                     >  4:226365/1‑124 (MQ=255)
                                                                                                                            ataaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                      >  7:90414/8‑149 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 <  7:50095/138‑1 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 >  8:50095/1‑138 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                      <  7:411835/149‑1 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGTGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                     >  4:78896/1‑149 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                     <  8:170757/149‑1 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                     >  4:51264/1‑149 (MQ=255)
                                                                                                                             taaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                     >  1:365636/7‑149 (MQ=255)
                                                                                                                               aaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtgg                                       >  3:10381/5‑129 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                  <  5:68069/149‑1 (MQ=255)
                                                                                                                                aaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                    >  2:127454/4‑147 (MQ=255)
                                                                                                                                 ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                    >  1:223627/1‑146 (MQ=255)
                                                                                                                                 ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAAGGCTCCGGGCAGGGCGGGATACTTAATCTGGCAATTAACGCAAAACCATCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                    >  8:421354/1‑146 (MQ=255)
                                                                                                                                       gccgGGGTCACACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                             <  6:334297/131‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCTGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa          <  3:146160/149‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa          <  7:439337/149‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 >  8:153358/1‑120 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                 <  7:153358/120‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATg    <  7:355714/149‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATg    <  1:251762/149‑1 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc             >  6:15517/1‑138 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc             <  5:15517/138‑1 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATggg  >  1:439292/1‑149 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGAACACGCTCGAGGCGTTAGAACCTAACAGATgg   <  5:258352/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGG  >  NC_000913/641217‑641509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: