Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 641,363 A→C 5.6% intergenic (+45/+76) ahpF → / ← uspG alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913641,3630AC 5.6% 446.2 / 16.5 143intergenic (+45/+76)ahpF/uspGalkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding/universal stress protein UP12
Reads supporting (aligned to +/- strand):  ref base A (70/65);  new base C (5/3);  total (75/68)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.15e-01

Only 100 of 151 total aligned reads displayed.

TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTC  >  NC_000913/641217‑641511
                                                                                                                                                  |                                                                                                                                                    
tGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAAcc                                                                                                                                                    >  5:315090/1‑149 (MQ=255)
  tACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTg                                                                                                                                                  >  7:349060/1‑149 (MQ=255)
           tCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCtttt                                                                                                                                         >  4:70510/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                      >  6:253395/1‑149 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                      <  8:158689/149‑1 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                      <  1:278579/149‑1 (MQ=255)
              gTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTa                                                                                                                                      >  5:216675/1‑149 (MQ=255)
               tACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTAt                                                                                                                                     >  3:282103/1‑149 (MQ=255)
                 cAATCATATCATCATCGCAAATGGCGAAGGTTCCAAAGCATATCTTATTGCTTTTGACTACCTTATTCGCACCAACACTGCATAAGAAGAAGTAAGATTAAACTGCAATTGCTTAGCCGACGGTGTCAAACCTGGCGGCTTTTTTATgg                                                                                                                                   <  5:31789/149‑1 (MQ=255)
                    gCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCAt                                                                                                                                <  6:216675/149‑1 (MQ=255)
                     cAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                                <  1:286906/132‑1 (MQ=255)
                       tatcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTa                                                                                                                              >  7:332710/2‑148 (MQ=255)
                        atcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTaa                                                                                                                             >  2:310424/1‑148 (MQ=255)
                         tcatcatcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTaaaa                                                                                                                           <  6:315090/149‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                            <  5:387273/127‑1 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCt                                                                                                                                            >  6:387273/1‑127 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGccc                                                                                                                      >  1:374362/1‑149 (MQ=255)
                              atcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGccc                                                                                                                      >  7:51923/1‑149 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                     >  8:355714/1‑149 (MQ=255)
                               tcGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGcccc                                                                                                                     >  2:251762/1‑149 (MQ=255)
                                   cACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGACGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgccg                                                                                                                 <  1:169965/149‑1 (MQ=255)
                                   cACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCccgccg                                                                                                                 >  7:179188/1‑149 (MQ=255)
                                      tGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGa                                                                                                              >  2:279396/1‑149 (MQ=255)
                                        gCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGAt                                                                                                             <  3:70510/148‑1 (MQ=255)
                                          gAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGc                                                                                                          >  7:35073/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTggc                                                                                                                                                <  5:265856/102‑1 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                                 >  4:146160/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                                 >  1:106578/1‑149 (MQ=255)
                                                   aaaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAg                                                                                                 >  4:428952/1‑149 (MQ=255)
                                                   aaaGCCGCTCTGAGTGCCTTTGACTACCTGATTCCCACCAAAACTGCATAAGAAGAACTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGGCAAACCTGTCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCGCCGGTCAg                                                                                                 <  6:367143/149‑1 (MQ=255)
                                                    aaGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAgg                                                                                                <  8:332710/149‑1 (MQ=255)
                                                     aGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAggg                                                                                               >  1:302883/1‑149 (MQ=255)
                                                     aGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAggg                                                                                               <  7:420640/149‑1 (MQ=255)
                                                       ccTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCg                                                                                             >  3:336491/1‑149 (MQ=255)
                                                          ctctGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                          >  4:147259/1‑149 (MQ=255)
                                                                 tGCTTTTGACTACCTGATTCGCACCAAAAATGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTAt                                                                                   >  4:316069/1‑149 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGa                                                                                          <  6:448216/139‑1 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCt                                                                                >  4:98714/1‑149 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCt                                                                                >  7:135437/1‑149 (MQ=255)
                                                                    ttttGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCt                                                                                >  2:390800/1‑149 (MQ=255)
                                                                        gACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGc                                                                             <  4:282103/148‑1 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                              >  5:201390/1‑144 (MQ=255)
                                                                           tACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTgg                                                                              <  6:201390/144‑1 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                       <  4:336491/149‑1 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                       >  4:220416/1‑149 (MQ=255)
                                                                             ccTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTaa                                                                       >  1:447631/1‑149 (MQ=255)
                                                                              cTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAAc                                                                      <  1:279396/149‑1 (MQ=255)
                                                                                gATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAg                                                                                                                         <  1:19621/96‑1 (MQ=255)
                                                                                     gCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                               <  6:452518/149‑1 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAATGCACAAc                                                               >  4:244067/1‑148 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAcc                                                              <  3:428952/149‑1 (MQ=255)
                                                                                      cACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAACCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                               >  8:414754/1‑148 (MQ=255)
                                                                                       aCCAAAACTGCGTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAGAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                               >  3:332042/1‑147 (MQ=255)
                                                                                       aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                               >  4:316729/1‑147 (MQ=255)
                                                                                       aCCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAAc                                                               >  6:258352/1‑147 (MQ=255)
                                                                                           aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAc                                                         <  8:51923/149‑1 (MQ=255)
                                                                                           aaaCTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCAc                                                         >  1:401255/1‑149 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                      >  2:7904/1‑148 (MQ=255)
                                                                                               tGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                      >  2:448860/1‑148 (MQ=255)
                                                                                                 cATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAg                                                   <  3:316069/149‑1 (MQ=255)
                                                                                                  aTAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCgg                                                      >  1:442016/1‑145 (MQ=255)
                                                                                                       aagaagTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggc                                             <  3:98714/149‑1 (MQ=255)
                                                                                                           agTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtg                                          <  2:442016/148‑1 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                        >  3:198258/1‑149 (MQ=255)
                                                                                                            gTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggc                                        <  1:7904/149‑1 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACaa                                                                >  2:283934/1‑124 (MQ=255)
                                                                                                             tAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATtggcgtggcg                                       >  2:464369/1‑149 (MQ=255)
                                                                                                                gATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATAc                                                                                       >  3:356176/1‑98 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                   <  3:220416/149‑1 (MQ=255)
                                                                                                                 aTTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCACCACCGGCAGATTGGCGTGGCGGATc                                   <  5:341418/149‑1 (MQ=255)
                                                                                                                  ttCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCa                                  <  2:302883/149‑1 (MQ=255)
                                                                                                                     aCCTGCAATAGATTAGCCGCTGGGGTCAAACATGCCGGCTTTTTTATCGCAATAAAAAGCCCCGCCGGGATGGCTCCCGGCAGGGCGGAATAATTAATCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGTCGGATAACGc                               >  8:313007/1‑149 (MQ=255)
                                                                                                                      ccTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCg                                                       >  4:226365/1‑124 (MQ=255)
                                                                                                                       cTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTc                             <  8:349060/149‑1 (MQ=255)
                                                                                                                          cAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAg                          >  8:411835/1‑149 (MQ=255)
                                                                                                                            ataaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                        >  1:24804/8‑149 (MQ=255)
                                                                                                                            ataaaaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                        >  7:90414/8‑149 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                        <  1:71406/149‑1 (MQ=255)
                                                                                                                            aTTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGc                        <  7:411835/149‑1 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGTGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                       >  4:78896/1‑149 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                       >  4:51264/1‑149 (MQ=255)
                                                                                                                             ttGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCg                       <  8:170757/149‑1 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                      >  7:393734/1‑147 (MQ=255)
                                                                                                                                cTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                    <  5:68069/149‑1 (MQ=255)
                                                                                                                                aaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                      >  2:127454/4‑147 (MQ=255)
                                                                                                                                aaaaGCCGCCAGGTTTGACCCCGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTa                    <  2:24804/146‑1 (MQ=255)
                                                                                                                                 ttAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGt                      >  1:223627/1‑146 (MQ=255)
                                                                                                                                     ccgccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc               >  8:439337/1‑149 (MQ=255)
                                                                                                                                       gccgGGGTCACACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGc                               <  6:334297/131‑1 (MQ=255)
                                                                                                                                       gccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTa             <  2:106578/149‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCTGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa            <  3:146160/149‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa            <  7:439337/149‑1 (MQ=255)
                                                                                                                                        ccgGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTaa            <  3:147259/149‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATc                                   <  7:153358/120‑1 (MQ=255)
                                                                                                                                              tCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATg      <  1:251762/149‑1 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc               >  6:15517/1‑138 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAAcc               <  5:15517/138‑1 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCGCGAGGCGTTAGAACCTAACAGATgg     <  2:86935/148‑1 (MQ=255)
                                                                                                                                                aaaCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGAACACGCTCGAGGCGTTAGAACCTAACAGATgg     <  5:258352/148‑1 (MQ=255)
                                                                                                                                                  aCCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCCGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTc  >  8:449401/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGTACGACGGTTCCGTACAAGCAGATCATCATCGCCACTGGCGAAGGTGCCAAAGCCTCTCTGAGTGCTTTTGACTACCTGATTCGCACCAAAACTGCATAAGAAGAAGTAAGATTCACCTGCAATTGCTTAGCCGCCGGGGTCAAACCTGGCGGCTTTTTTATGGCATTAAAAAGCCCCGCCGGGATGGCTCCGGGCAGGGCGGGATACTTATTCTGGCAATTAACGCACAACCAGCACCGGCAGATTGGCGTGGCGGATCACGCTCGAGGCGTTAGAACCTAACAGATGGGTC  >  NC_000913/641217‑641511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: