Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 670,215 A→G 5.6% Y120H (TAC→CAC)  nadD ← nicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913670,2150AG 5.6% 218.3 / 2.4 71Y120H (TAC→CAC) nadDnicotinic acid mononucleotide adenylyltransferase, NAD(P)‑dependent
Reads supporting (aligned to +/- strand):  ref base A (35/32);  new base G (2/2);  total (37/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAG  >  NC_000913/670068‑670359
                                                                                                                                                   |                                                                                                                                                
ttttACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACAATCAAATGTGCATTGTCGAGTATCGTttcgtat                                                                                                                                                 <  2:340823/149‑1 (MQ=255)
ttttACCGGCAGGCTGAAGGTGAAGATCTACCGCCTTATGTGTCTAAAGATCTTCCAGCCATTGCAGGTATTGCGGTTCCGCCAATTCAAGTGCGAAACCTCGACGCCGACACCCGATCAAAAGAGCATTCTCCAGATTCGTtgcgta                                                                                                                                                  >  4:311819/1‑148 (MQ=255)
   tACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtattc                                                                                                                                               <  7:153552/148‑1 (MQ=255)
    aCCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtattcg                                                                                                                                              <  1:160338/148‑1 (MQ=255)
     ccGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtattcgta                                                                                                                                            <  8:271841/149‑1 (MQ=255)
      cGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTAc                                                                                                                                           <  4:9122/149‑1 (MQ=255)
               gAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCgg                                                                                                                                   >  2:25845/1‑148 (MQ=255)
                 aGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGaaa                                                                                                                                <  2:3451/149‑1 (MQ=255)
                   gTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGAATTGCGGTTGCGCCATTTCAAGTGGCTCACCTGGCCGCCGACAGACGATCAAATGTGCATTTTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAg                                                                                                                               >  1:244422/1‑148 (MQ=255)
                    tGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGt                                                                                                                             <  2:348245/149‑1 (MQ=255)
                       aGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTcag                                                                                                                          <  1:25845/149‑1 (MQ=255)
                        gATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTcag                                                                                                                          >  5:155645/1‑148 (MQ=255)
                            ttCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtatt                                                                                                                                                >  2:105032/1‑122 (MQ=255)
                            ttCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTttcgtatt                                                                                                                                                <  1:105032/122‑1 (MQ=255)
                            ttCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGt                                                                                                                      >  2:335756/1‑148 (MQ=255)
                            ttCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGt                                                                                                                      >  1:273079/1‑148 (MQ=255)
                              ccGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTg                                                                                                                     <  8:130754/147‑1 (MQ=255)
                                   ttATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCt                                                                                                               >  1:295163/1‑148 (MQ=255)
                                          tCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaat                                                                                                        <  3:57478/148‑1 (MQ=255)
                                           cAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTg                                                                                                              >  8:30137/1‑141 (MQ=255)
                                           cAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTg                                                                                                              <  7:30137/141‑1 (MQ=255)
                                            aaaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCaataa                                                                                                      >  3:96200/1‑148 (MQ=255)
                                            aaaTGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTAAGCAGTTAATCCTGACCaataa                                                                                                      >  8:72784/1‑148 (MQ=255)
                                               tGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAAtaataag                                                                                                  <  5:74249/149‑2 (MQ=255)
                                                 aTCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACg                                                                                                >  1:240842/1‑149 (MQ=255)
                                                    ttCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCTAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGc                                                                                               >  2:149162/1‑147 (MQ=255)
                                                      ccAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGc                                                                                           >  3:29638/1‑149 (MQ=255)
                                                         gCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGc                                                                                        <  6:444786/149‑1 (MQ=255)
                                                           aaTTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCAc                                                                                      <  4:29638/148‑1 (MQ=255)
                                                                 tGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCgg                                                                                >  6:178540/1‑149 (MQ=255)
                                                                   gTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGt                                                                               <  5:178540/148‑1 (MQ=255)
                                                                    tATTGCGGTTGCGCCAGTTCAAGTGGGTAACCTGGACGCCGAGAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGGCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTcc                                                                             <  2:244422/149‑1 (MQ=255)
                                                                     aTTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCt                                                                            >  7:369019/1‑149 (MQ=255)
                                                                     aTTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCt                                                                            >  7:170943/1‑149 (MQ=255)
                                                                      ttGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTGTTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCtt                                                                           >  5:386991/1‑149 (MQ=255)
                                                                      ttGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACTCCAGCGGCACGTCCGGTCCt                                                                            <  7:72784/148‑1 (MQ=255)
                                                                      ttGCGGTTGCGCCATTTCAAGTGGGTAAACTGTAATCCGACACACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCATTACCAATAATAAACGACATCGTCACGTCAGGTCCtt                                                                           >  8:237473/1‑149 (MQ=255)
                                                                         cGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGt                                                                         <  1:335756/148‑1 (MQ=255)
                                                                         cGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGGCAGCAGTGAATCCTGACCAATAATAAACGCCATCGGCACGTCCGGTCCTTGtt                                                                        <  6:155645/149‑1 (MQ=255)
                                                                          ggTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTc                                                                                   <  4:207038/137‑1 (MQ=255)
                                                                          ggTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTc                                                                                   >  3:207038/1‑137 (MQ=255)
                                                                          ggTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTc                                                                       <  8:369019/149‑1 (MQ=255)
                                                                           gTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTca                                                                                                                           <  7:177442/96‑1 (MQ=255)
                                                                           gTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTca                                                                                                                           >  8:177442/1‑96 (MQ=255)
                                                                            atccgcCAATCCAAGTGGGTAACCAGGACGCCGACACACGAGCAAATCTGCAATGTCGAGCATCGTATCGTGTTCGTACCAGGTCGGAAACGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCt                                                                     <  6:386991/146‑1 (MQ=255)
                                                                                gcCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTgccg                                                                 >  6:429163/1‑149 (MQ=255)
                                                                                gcCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTgccg                                                                 >  3:394522/1‑149 (MQ=255)
                                                                                 ccATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTgccgc                                                                >  8:270006/1‑149 (MQ=255)
                                                                                        aaGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTGTTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCActct                                                          <  5:177846/148‑1 (MQ=255)
                                                                                        aaGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTGTTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCActct                                                          >  6:177846/1‑148 (MQ=255)
                                                                                          gTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTc                                                       <  2:295163/149‑1 (MQ=255)
                                                                                           tGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCa                                                      <  2:273079/149‑1 (MQ=255)
                                                                                             ggTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAgt                                                    <  1:328620/149‑1 (MQ=255)
                                                                                             ggTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAgt                                                    <  5:429163/149‑1 (MQ=255)
                                                                                             ggTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAg                                                     <  4:96200/148‑1 (MQ=255)
                                                                                                aaCCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGtt                                                 >  7:234019/1‑149 (MQ=255)
                                                                                                  ccTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAACCGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTg                                               >  6:290360/1‑149 (MQ=255)
                                                                                                         gCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGATTCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAgtg                                        >  4:31664/1‑149 (MQ=255)
                                                                                                            gACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCa                                                      >  4:732/1‑132 (MQ=255)
                                                                                                            gACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCa                                                      <  3:732/132‑1 (MQ=255)
                                                                                                            gACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTAACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAgtgt                                       >  3:385662/1‑147 (MQ=255)
                                                                                                               aGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAagag                                  <  7:270006/149‑1 (MQ=255)
                                                                                                                 acgttaaaaTGTGCATTGTCTAGTATCGTTTCGTATTGTTACCAGGTCGTAAAGCTCAGCAGTGAATCCTGACCAATATTAAACGCCTGCTGAACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAggg                                <  8:404607/143‑1 (MQ=255)
                                                                                                                 aCGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAggg                                <  4:394522/149‑1 (MQ=255)
                                                                                                                 aCGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATACTGACCAATAATAAACGCCAGCGGCCCGTCCGGTCCTTGTTACTGCCGCCACTCTTTCACTGTTTTCGCa                                           >  3:24803/1‑138 (MQ=255)
                                                                                                                 aCGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACAAGGTCGGAAAGGTCAGCAGTGAATCATGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAggg                                >  6:257791/1‑149 (MQ=255)
                                                                                                                   gATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAagag                                  <  3:384768/145‑1 (MQ=255)
                                                                                                                   gATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAagag                                  >  4:384768/1‑145 (MQ=255)
                                                                                                                     tCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTc                                                       <  5:172050/122‑1 (MQ=255)
                                                                                                                     tCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTc                                                       >  6:172050/1‑122 (MQ=255)
                                                                                                                        aaTGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAg                                                     >  8:104771/1‑121 (MQ=255)
                                                                                                                        aaTGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAg                                                     <  7:104771/121‑1 (MQ=255)
                                                                                                                            tgCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGttt                                                >  7:73728/1‑122 (MQ=255)
                                                                                                                            tgCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGttt                                                <  8:73728/122‑1 (MQ=255)
                                                                                                                                   tCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTc              >  3:66314/1‑149 (MQ=255)
                                                                                                                                      aGTATCGTTTCGTAGTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGttt                                                >  8:122098/1‑112 (MQ=255)
                                                                                                                                            gTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc     <  4:66314/149‑1 (MQ=255)
                                                                                                                                             tttCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc     >  4:401474/1‑148 (MQ=255)
                                                                                                                                              ttcgtattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTtcatc     >  3:300134/1‑147 (MQ=255)
                                                                                                                                               tcgtattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCaa   <  2:240842/148‑1 (MQ=255)
                                                                                                                                                cgtattcgtaCCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAag  <  8:170943/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTTTACCGGCAGGCTGAAGGTGAAGATCTTCCGGGTTATGTGTCAAATGATCTTCCAGCCATTGCTGGTATTGCGGTTGCGCCATTTCAAGTGGGTAACCTGGACGCCGACAGACGATCAAATGTGCATTGTCGAGTATCGTTTCGTATTCGTACCAGGTCGGAAAGGTCAGCAGTGAATCCTGACCAATAATAAACGCCAGCGGCACGTCCGGTCCTTGTTCCTGCCGCCACTCTTTCAGTGTTTGCGCAGTGTAAGAGGGGGCATTGCGCTTTAGCTCGCGTTCATCAAG  >  NC_000913/670068‑670359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: