Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 909,353 G→A 69.9% A566V (GCG→GTG)  poxB ← pyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913909,3530GA69.9% 112.6 / 63.7 83A566V (GCG→GTG) poxBpyruvate dehydrogenase, thiamine triphosphate‑binding, FAD‑binding
Reads supporting (aligned to +/- strand):  ref base G (10/15);  new base A (24/34);  total (34/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGAC  >  NC_000913/909209‑909494
                                                                                                                                                |                                                                                                                                             
aCGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAg                                                                                                                                           >  6:242497/1‑149 (MQ=255)
aCGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGAATGTCCTTATTATGACGGGAAATGCAACCATTTTTACCTTAGACAGTTTGTTTTCACCAg                                                                                                                                           >  2:376330/1‑149 (MQ=255)
     gtcCCCAACCGGGGAGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACAAGTTCg                                                                                                                                       >  3:373181/1‑148 (MQ=255)
      tcCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGAt                                                                                                                                     <  5:222700/149‑1 (MQ=255)
        cccAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCa                                                                                                                                   <  4:174582/149‑1 (MQ=255)
         ccAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCAc                                                                                                                                  >  8:235235/1‑149 (MQ=255)
            aCCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtc                                                                                                                               <  7:194381/149‑1 (MQ=255)
               ggggCGGACATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCTCTGCGTAAATCAATCATGGCATGTCCTTATTATGTCGGTAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTGACCAGTTCGATCACTtcatc                                                                                                                            <  8:254700/149‑1 (MQ=255)
                 ttctgcCATCATCGATTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCAGTGCGTAAATCAATCTTTGCATGTCCTTTTTATGACGTTAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTtcat                                                                                                                             <  3:334/142‑1 (MQ=255)
                  gcggcCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCAcc                                                                                                                         <  6:138784/149‑1 (MQ=255)
                   cggcCATCATTGCTTCGAGCTTGGCGCGGCTTGGTAGGGTATAGGTATGTCTTCGTAATTCATTAATTGCATTTTATTATTATGTCTGGAAATGCCACCCTTTTTTCCTTATGCAGTTTGTTTTCGGCTGTTGGATCTGTTGATCAcgt                                                                                                                        <  6:415644/149‑3 (MQ=255)
                      ccATCATCGCTTCGAGCATGGAGCGCCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGTCATGTCCTTAATATGAACCGAAATTCCACCCATTTTACCTAAGCCAGATTGTTTTCGCAAGTTACATCACTtcatcagatag                                                                                                                      >  4:334/1‑143 (MQ=255)
                          catcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGc                                                                                                                 >  1:287916/1‑149 (MQ=255)
                           atcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGc                                                                                                                 <  4:342631/148‑1 (MQ=255)
                           atcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGc                                                                                                                 <  8:22189/148‑1 (MQ=255)
                           atcGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCt                                                                                                                >  7:92530/1‑149 (MQ=255)
                              gCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtga                                                                                                              <  1:273704/148‑1 (MQ=255)
                                ttCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCtgatga                                                                                                           >  1:308821/1‑149 (MQ=255)
                                    aGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgc                                                                                                       <  1:211630/149‑1 (MQ=255)
                                    aGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgc                                                                                                       >  7:143087/1‑149 (MQ=255)
                                    aGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgc                                                                                                       <  8:92530/149‑1 (MQ=255)
                                          gcgcGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCCTGGCAGGGCCTTATTATGCCGGGAAATGCCACCCTGTTTACCTTCGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGGCCGCTGATGATTGCGCgcag                                                                                                 <  1:399238/149‑1 (MQ=255)
                                          gcgcGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgca                                                                                                  <  2:149428/148‑1 (MQ=255)
                                          gcgcGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgcag                                                                                                 >  6:398173/1‑149 (MQ=255)
                                          gcgcGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgca                                                                                                  <  7:235235/148‑1 (MQ=255)
                                          gcgcGGCTTGGGCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACTGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgcag                                                                                                 <  1:405628/149‑1 (MQ=255)
                                           cgcgGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCgcagc                                                                                                <  5:242497/149‑1 (MQ=255)
                                               gCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCatat                                                                                            <  6:395325/149‑1 (MQ=255)
                                                cTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                                                                                                     >  8:164417/1‑139 (MQ=255)
                                                cTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTgcgc                                                                                                     <  7:164417/139‑1 (MQ=255)
                                                    gTCGTGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAgg                                                                                       <  1:376330/149‑1 (MQ=255)
                                                     tCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGc                                                                                      <  7:409740/149‑1 (MQ=255)
                                                          tAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGtt                                                                                                                                         <  5:433716/93‑1 (MQ=255)
                                                          tAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGtt                                                                                                                                         >  6:433716/1‑93 (MQ=255)
                                                               gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCttt                                                                            <  6:73257/149‑1 (MQ=255)
                                                               gTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCttt                                                                            <  2:274555/149‑1 (MQ=255)
                                                                tATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTg                                                                           <  5:398173/149‑1 (MQ=255)
                                                                  tCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAata                                                                                             <  1:88512/129‑1 (MQ=255)
                                                                  tCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAata                                                                                             >  2:88512/1‑129 (MQ=255)
                                                                     cTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTg                                                                      >  2:115573/1‑149 (MQ=255)
                                                                        cGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGtt                                                                    <  8:239692/148‑1 (MQ=255)
                                                                         gTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGGCCGCTGATTATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTc                                                                   <  4:349874/148‑1 (MQ=255)
                                                                          tAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTc                                                                   >  2:231971/1‑147 (MQ=255)
                                                                             atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                              >  2:35276/1‑149 (MQ=255)
                                                                             atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                              <  1:231971/149‑1 (MQ=255)
                                                                             atcaatcaTGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGtt                                                              >  3:113368/1‑149 (MQ=255)
                                                                                     tGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCt                                                        >  4:59019/1‑147 (MQ=255)
                                                                                       gCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCGTGTTCGAGTTTGATCTGCg                                                     <  2:14859/148‑1 (MQ=255)
                                                                                        cATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGt                                                   <  8:116580/149‑1 (MQ=255)
                                                                                           gTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTgg                                                 >  3:133051/1‑148 (MQ=255)
                                                                                            tCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGaa                                               <  8:86644/149‑1 (MQ=255)
                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCtgatga                                                                                                           <  8:407091/87‑1 (MQ=255)
                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCtgatga                                                                                                           >  7:407091/1‑87 (MQ=255)
                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAAt                                              <  4:113368/148‑1 (MQ=255)
                                                                                              cTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATg                                             >  7:429617/1‑149 (MQ=255)
                                                                                               ttattaTGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATAACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATgg                                            >  7:227984/1‑149 (MQ=255)
                                                                                               ttattaTGACGGGAAATGCCACCCTTTTTACCCTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATgg                                            >  3:114559/1‑149 (MQ=255)
                                                                                                 attatGACGGGAAATGCCACCCTTTTTACCTTATCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAACCCTTTGGCCTGTTCGAGTTTGATCTTCGGTTGAATGGc                                           >  2:363008/1‑148 (MQ=255)
                                                                                                    atGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGCCCTGTTCGAGTTTGATCTGCGGTGGAAt                                              >  5:97377/1‑142 (MQ=255)
                                                                                                    atGACGGGAAATGCCAACCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAAt                                              <  6:97377/142‑1 (MQ=255)
                                                                                                     tGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAAc                                       <  8:143087/148‑1 (MQ=255)
                                                                                                     tGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg                                                  <  8:461100/137‑1 (MQ=255)
                                                                                                     tGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTg                                                  >  7:461100/1‑137 (MQ=255)
                                                                                                     tGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTACGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTCGCCTGTTCGAGTATGATCTGCGGTGGAATGGCAAAct                                      >  6:39896/1‑149 (MQ=255)
                                                                                                          ggAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACtcttct                                 <  8:429617/149‑1 (MQ=255)
                                                                                                             aaTGCCACCCTTTTTACCTTAGCGAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTTATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                              <  2:287916/149‑1 (MQ=255)
                                                                                                             aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                              <  4:133051/149‑1 (MQ=255)
                                                                                                             aaTGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTg                              <  8:98623/149‑1 (MQ=255)
                                                                                                                   aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                           >  3:78129/1‑130 (MQ=255)
                                                                                                                   aCCCTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGc                                           <  4:78129/130‑1 (MQ=255)
                                                                                                                    cccTTTTTACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacca                       >  7:86648/1‑149 (MQ=255)
                                                                                                                       tttttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                 <  6:304946/104‑1 (MQ=255)
                                                                                                                       tttttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGa                                                                 >  5:304946/1‑104 (MQ=255)
                                                                                                                       tttttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGAccacca                    >  6:76273/1‑149 (MQ=255)
                                                                                                                          ttACCTTAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACcaca                  <  2:308821/148‑1 (MQ=255)
                                                                                                                                tAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc                        <  5:151098/136‑1 (MQ=255)
                                                                                                                                tAGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGacc                        >  6:151098/1‑136 (MQ=255)
                                                                                                                                 aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacca           <  3:59019/148‑1 (MQ=255)
                                                                                                                                 aGCCAGTTTGTTTTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATccacca           <  8:86648/148‑1 (MQ=255)
                                                                                                                                          gtttTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGAATATACATTCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGAc  >  4:78895/1‑148 (MQ=255)
                                                                                                                                          gtttTCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGAc  <  1:363008/148‑1 (MQ=255)
                                                                                                                                           ttttCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCt                                                                              >  3:104605/1‑71 (MQ=255)
                                                                                                                                           ttttCACCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCt                                                                              <  4:104605/71‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                             
ACGTCGTCCCCAACCGGGGCGGCCATCATCGCTTCGAGCATGGCGCGGCTTGGTCGGGTAACGGTATCACTGCGTAAATCAATCATGGCATGTCCTTATTATGACGGGAAATGCCACCCTTTTTACCTTAGCCAGTTTGTTTTCGCCAGTTCGATCACTTCATCACCGCGTCCGCTGATGATTGCGCGCAGCATATACAGGCTGAAACCTTTGGCCTGTTCGAGTTTGATCTGCGGTGGAATGGCTAACTCTTCTTTGGCGACCACCACATCCACCAACACCGGAC  >  NC_000913/909209‑909494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: