Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,159,287 C→A 100% Y473* (TAC→TAA)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,159,2870CA100.0% 325.2 / NA 92Y473* (TAC→TAA) ptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (48/44);  total (48/44)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGG  >  NC_000913/1159144‑1159432
                                                                                                                                               |                                                                                                                                                 
cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGTTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAAt                                                                                                                                                 <  6:221941/146‑1 (MQ=255)
cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATcc                                                                                                                                               <  2:397070/148‑1 (MQ=255)
cgcgTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATcc                                                                                                                                               <  1:406957/148‑1 (MQ=255)
cgcgCCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATcc                                                                                                                                               <  7:109537/148‑1 (MQ=255)
      aGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCa                                                                                                                                        >  6:433775/1‑149 (MQ=255)
              tGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                                >  5:32568/1‑149 (MQ=255)
               gATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCg                                                                                                                                >  2:226792/1‑148 (MQ=255)
                aTGTGTCTAATGTGTATCAGGCCTGCCTGAAGAAACTGGGCGAAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTa                                                                                                                              <  3:12619/149‑1 (MQ=255)
                aTGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGt                                                                                                                               >  1:223700/1‑148 (MQ=255)
                     tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACg                                                                                                                         >  1:3930/1‑149 (MQ=255)
                     tCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACg                                                                                                                         >  1:107215/1‑149 (MQ=255)
                       tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGtt                                                                                                                       >  6:101484/1‑149 (MQ=255)
                       tAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGtt                                                                                                                       >  1:208301/1‑149 (MQ=255)
                         aaGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTgg                                                                                                                     <  1:414850/149‑1 (MQ=255)
                          aGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTggt                                                                                                                    <  2:265903/149‑2 (MQ=255)
                          aGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTggg                                                                                                                    <  5:343354/149‑1 (MQ=255)
                            tGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGga                                                                                                                  <  7:303887/149‑1 (MQ=255)
                              gATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                                >  7:439103/1‑149 (MQ=255)
                               aTCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGa                                                                                                                           >  4:337069/1‑137 (MQ=255)
                               aTCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGa                                                                                                                           <  3:337069/137‑1 (MQ=255)
                               aTCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGgaga                                                                                                                >  1:435486/1‑148 (MQ=255)
                                  aGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTaa                                                                                                            <  2:107215/149‑1 (MQ=255)
                                          cTGAAGAAACTGGGCGCATCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCATGCGATTTTCGGTACTAAATAAGATAACCTGAAAACCGAGATGGATGAGTAAATCAGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAga                                                                                                      >  8:372542/1‑146 (MQ=255)
                                          cTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                                      >  4:403125/1‑147 (MQ=255)
                                           tGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGc                                                                                                      >  7:422608/1‑146 (MQ=255)
                                              agaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATg                                                                                                <  8:350852/149‑1 (MQ=255)
                                               gaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATgg                                                                                               >  4:21221/1‑149 (MQ=255)
                                                  aCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGGGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTg                                                                                            >  8:381668/1‑149 (MQ=255)
                                                  aCTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAAACGTTGGGGAGACTAAGGCAGCCAGATGGCTg                                                                                            >  8:380500/1‑149 (MQ=255)
                                                       gcgcAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttt                                                                                       <  2:208301/149‑1 (MQ=255)
                                                         gctgCGGGCGTAGTGGTTGCGGGTGCTTGTGGTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAACACCGAGATGGATGAGTAATTCCGTAACCACTAATCCGTAAGAAGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttttt                                                                                     <  4:63960/146‑1 (MQ=255)
                                                           aGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTa                                                                                   <  3:391989/149‑1 (MQ=255)
                                                             cGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAAACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACa                                                                                 <  7:380500/149‑1 (MQ=255)
                                                              gtgCGTAGTGTTTGCTGGTTCTGGTGTTCAGTCTATTTTCGGTACTAAATCCGATAACATGAAAACCTATATTGATGAGTAATTTCGAAACCACTAATCGGTAAGTCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACa                                                                                 <  6:199256/146‑1 (MQ=255)
                                                              gggCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                                >  1:376943/1‑149 (MQ=255)
                                                                   tAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTTATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGgtgt                                                                            >  2:184348/1‑148 (MQ=255)
                                                                   tAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGtt                                                                           <  3:403125/149‑1 (MQ=255)
                                                                     gTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTa                                                                          <  6:357726/148‑1 (MQ=255)
                                                                     gTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAt                                                                         >  4:179107/1‑149 (MQ=255)
                                                                      tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAtt                                                                        <  4:176416/149‑1 (MQ=255)
                                                                      tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAtt                                                                        <  8:422608/149‑1 (MQ=255)
                                                                      tGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAt                                                                         >  5:113086/1‑148 (MQ=255)
                                                                        gTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCa                                                                      <  2:376943/149‑1 (MQ=255)
                                                                            cTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTa                                                                                   <  4:237683/132‑1 (MQ=255)
                                                                            cTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTa                                                                                   >  3:237683/1‑132 (MQ=255)
                                                                               gTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGGCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                               <  5:101484/149‑1 (MQ=255)
                                                                               gTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                               >  5:411834/1‑149 (MQ=255)
                                                                                ttCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                              <  6:411834/149‑1 (MQ=255)
                                                                                  cTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                            >  8:88969/1‑149 (MQ=255)
                                                                                   tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                              <  8:184492/146‑1 (MQ=255)
                                                                                   tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGAt                                                              >  7:184492/1‑146 (MQ=255)
                                                                                     gtgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                            <  7:95689/146‑1 (MQ=255)
                                                                                        ttCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                                      >  5:267389/1‑149 (MQ=255)
                                                                                          cAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCgg                                                     <  6:32568/148‑1 (MQ=255)
                                                                                          cAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCgg                                                     <  6:113086/148‑1 (MQ=255)
                                                                                           aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTCACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTGTTACAGGTGTTATTCAGAAGTGATACGGGCCGGt                                                    >  2:89256/1‑148 (MQ=255)
                                                                                           aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                    >  6:28453/1‑148 (MQ=255)
                                                                                           aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                    >  4:136829/1‑148 (MQ=255)
                                                                                           aGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGt                                                    >  4:100119/1‑148 (MQ=255)
                                                                                            ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                                  <  5:433775/149‑1 (MQ=255)
                                                                                             gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAAt                                                 >  4:276907/1‑149 (MQ=255)
                                                                                              cGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATg                                                <  3:276907/149‑1 (MQ=255)
                                                                                                aTTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCt                                              <  2:3930/149‑1 (MQ=255)
                                                                                                          cTAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgc                                    >  6:430134/1‑149 (MQ=255)
                                                                                                           tAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                                   >  1:21662/1‑149 (MQ=255)
                                                                                                           tAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                                   >  1:28749/1‑149 (MQ=255)
                                                                                                           tAAATCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                                   >  8:283695/1‑149 (MQ=255)
                                                                                                             aaTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGc                                               >  6:368059/1‑135 (MQ=255)
                                                                                                             aaTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGc                                               <  5:368059/135‑1 (MQ=255)
                                                                                                             aaTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                 >  6:406577/1‑149 (MQ=255)
                                                                                                             aaTCCGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                 <  3:100119/149‑1 (MQ=255)
                                                                                                                 cGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTACCCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                                <  6:358085/98‑1 (MQ=255)
                                                                                                                 cGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAg                                                                                >  5:358085/1‑98 (MQ=255)
                                                                                                                 cGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                                      >  8:282922/1‑124 (MQ=255)
                                                                                                                 cGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                                      <  7:282922/124‑1 (MQ=255)
                                                                                                                   aTAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCg                           <  1:184348/149‑1 (MQ=255)
                                                                                                                       ccTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGa                       <  2:223700/149‑1 (MQ=255)
                                                                                                                        cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATAc                                                            <  4:336759/111‑1 (MQ=255)
                                                                                                                        cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                      >  8:279694/1‑149 (MQ=255)
                                                                                                                        cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                      >  1:212357/1‑149 (MQ=255)
                                                                                                                        cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGAc                      >  7:419217/1‑149 (MQ=255)
                                                                                                                        cTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGATATTCAGAATTGAAACGTGCCGGTAATGCAGAAATTACGCGGTGTGCCGTAGAc                      >  1:85511/1‑149 (MQ=255)
                                                                                                                           aaacCCGAGATGGATGAGTAAATCCTTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGa                    <  7:372542/148‑1 (MQ=255)
                                                                                                                             aaCCGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAt                                                                         >  6:426225/1‑93 (MQ=255)
                                                                                                                                cGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAc              >  7:98213/1‑149 (MQ=255)
                                                                                                                                cGAGATGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAAc              <  7:88969/149‑1 (MQ=255)
                                                                                                                                  agaTGGATGAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCt            <  3:136829/149‑1 (MQ=255)
                                                                                                                                    atggatgAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTc          <  7:381668/149‑1 (MQ=255)
                                                                                                                                          gAGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGtt    >  8:432985/1‑149 (MQ=255)
                                                                                                                                           aGTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg   <  1:226792/149‑1 (MQ=255)
                                                                                                                                            gTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTgg  >  6:309169/1‑149 (MQ=255)
                                                                                                                                            gTAAATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTgg  >  7:305452/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
CGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGG  >  NC_000913/1159144‑1159432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑