Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,499,655 Δ1 bp 5.1% coding (985/1698 nt) ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,499,6550T. 5.1% 261.0 / 6.1 79coding (985/1698 nt)ypdAsensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
Reads supporting (aligned to +/- strand):  ref base T (38/37);  new base . (2/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCT  >  NC_000913/2499510‑2499800
                                                                                                                                                 |                                                                                                                                                 
cGGAAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTa                                                                                                                                                 >  2:297174/1‑148 (MQ=255)
          atcatTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGc                                                                                                                                      <  1:297174/149‑1 (MQ=255)
             gttAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggt                                                                                                                                   <  1:237735/148‑1 (MQ=255)
             gttAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggt                                                                                                                                   <  1:230721/148‑1 (MQ=255)
              ttAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggtc                                                                                                                                  <  5:158792/149‑1 (MQ=255)
                aaaaaCAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggtcgg                                                                                                                                >  7:432469/1‑149 (MQ=255)
                aaaaaCAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggtcg                                                                                                                                 <  8:432469/148‑1 (MQ=255)
                   aaCAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCt                                                                                                                             <  7:277165/149‑1 (MQ=255)
                         gatgaAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTAc                                                                                                                                                <  1:58392/124‑1 (MQ=255)
                         gatgaAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTAc                                                                                                                                                >  2:58392/1‑124 (MQ=255)
                            gaAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAat                                                                                                                    >  5:62448/1‑149 (MQ=255)
                                cccACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAatcatc                                                                                                                >  6:30872/1‑149 (MQ=255)
                                  cACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCAtctc                                                                                                              >  7:353719/1‑149 (MQ=255)
                                  cACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCAGTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATACAGGAAATGGCGGTCGGGCTGTCGCAAATCATCTcc                                                                                                             >  4:405501/1‑149 (MQ=255)
                                            cAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTg                                                                                                    >  1:83905/1‑149 (MQ=255)
                                                gaTTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAgg                                                                                                <  1:340285/149‑1 (MQ=255)
                                                 attcattcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGtt                                                                                              >  4:286406/1‑149 (MQ=255)
                                                  ttcattcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACa                                                                                                                                               >  7:358844/1‑100 (MQ=255)
                                                  ttcattcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACa                                                                                                                                               <  8:358844/100‑1 (MQ=255)
                                                     attcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGtttaa                                                                                           >  2:27014/1‑147 (MQ=255)
                                                     attcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCa                                                                                           <  7:374423/149‑1 (MQ=255)
                                                     attcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCa                                                                                           <  2:20718/149‑1 (MQ=255)
                                                     attcCATGCTGGAGATCCCATAGTGGGAGAAAGGGGTCGTGAACGTAACGCTCAAAATTTACTACTGCCCCGCGCATCAGAACACCTCGTCATTACAGCAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTATCa                                                                                           >  6:383385/1‑149 (MQ=255)
                                                      ttcCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCAc                                                                                          <  1:497643/149‑1 (MQ=255)
                                                              tGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGag                                                                                  >  4:43284/1‑149 (MQ=255)
                                                              tGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGag                                                                                  >  8:455162/1‑149 (MQ=255)
                                                                gTGATCCCATTGTGGGAGAAAGGGTTCGTAACCGGAACGATCAAAATTTACTAGTGCCACGCGCTGCAGAACCCCTCGTCATTACAGCAAATGGCGGTCGGAGTGTCGAAAAAAAACGCCACGCAACTGCACTTTACACCCGCCGagcc                                                                                >  8:472673/1‑148 (MQ=255)
                                                                gTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggtcggt                                                                                                                               >  3:321553/1‑102 (MQ=255)
                                                                gTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGcggtcggt                                                                                                                               <  4:321553/102‑1 (MQ=255)
                                                                    tCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTa                                                                            >  1:345849/1‑149 (MQ=255)
                                                                     cccATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTcc                                                                                                             <  8:172545/115‑1 (MQ=255)
                                                                     cccATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTcc                                                                                                             >  7:172545/1‑115 (MQ=255)
                                                                      ccATTTTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATCGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACg                                                                          >  2:413140/1‑149 (MQ=255)
                                                                      ccATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACg                                                                          >  8:251936/1‑149 (MQ=255)
                                                                      ccATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACg                                                                          >  2:463143/1‑149 (MQ=255)
                                                                       cATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGt                                                                         >  4:430935/1‑149 (MQ=255)
                                                                            tgGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGaaa                                                                     <  7:152019/148‑1 (MQ=255)
                                                                            tgGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAAt                                                                    <  7:391308/149‑1 (MQ=255)
                                                                               gagaAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCAGCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGc                                                                 <  2:318279/149‑1 (MQ=255)
                                                                                     ggggTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATaa                                                           <  7:374399/149‑1 (MQ=255)
                                                                                      gggTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAg                                                          <  7:251936/149‑1 (MQ=255)
                                                                                        gtagTGACAGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGTTCACCTAGTCATTACAGGATATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGATATgg                                                                  <  4:322159/136‑1 (MQ=255)
                                                                                        gTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATgg                                                                  >  3:322159/1‑139 (MQ=255)
                                                                                        gTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAgg                                                         >  4:29355/1‑148 (MQ=255)
                                                                                        gTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGc                                                        <  1:463143/149‑1 (MQ=255)
                                                                                         tCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCa                                                       <  3:430935/149‑1 (MQ=255)
                                                                                            tGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCagag                                                    <  5:30872/149‑1 (MQ=255)
                                                                                              acaggAACGCTGAAAATTTACTACTGCCACGCGCATCATAGCACCTCGTCATTACAGGAAATGCGGTCGGTCTGTCGCAAATCATCTCCACTCAACTGTAGGTTTCAAGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCt                                                  <  2:234942/145‑1 (MQ=255)
                                                                                               ccGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCtt                                                 <  7:4063/149‑1 (MQ=255)
                                                                                                cGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTc                                                >  8:283451/1‑149 (MQ=255)
                                                                                                        tGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCtgc                                                <  1:98275/141‑3 (MQ=255)
                                                                                                        tGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTc                                                >  2:98275/1‑141 (MQ=255)
                                                                                                        tGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTg                                        >  3:22514/1‑149 (MQ=255)
                                                                                                        tGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTg                                        >  5:414095/1‑149 (MQ=255)
                                                                                                        tGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTg                                        >  7:405892/1‑149 (MQ=255)
                                                                                                           aaaTTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTgcaa                                     <  3:43284/149‑1 (MQ=255)
                                                                                                             aTTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTgcaaag                                   <  7:472673/149‑1 (MQ=255)
                                                                                                                 actactGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGGCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTgcaaagcaa                                <  6:114637/148‑1 (MQ=255)
                                                                                                                   tactGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACAGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAAt                             >  1:317380/1‑149 (MQ=255)
                                                                                                                     ctGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTTGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAAtt                            >  4:94589/1‑148 (MQ=255)
                                                                                                                     ctGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAAt                             <  8:405892/147‑1 (MQ=255)
                                                                                                                      tGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTaa                          >  6:149120/1‑149 (MQ=255)
                                                                                                                        ccACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTaa                          <  2:83905/147‑1 (MQ=255)
                                                                                                                        ccACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATc                        >  8:310740/1‑149 (MQ=255)
                                                                                                                        ccACGCGCATCAGATCACCTCGTCATACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAAt                         <  3:286406/147‑1 (MQ=255)
                                                                                                                                aTCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCAtttt                >  7:231149/1‑149 (MQ=255)
                                                                                                                                  cAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCt              >  2:212157/1‑149 (MQ=255)
                                                                                                                                   aGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCt              <  7:267753/148‑1 (MQ=255)
                                                                                                                                   aGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGAAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTg             >  3:75723/1‑149 (MQ=255)
                                                                                                                                    gATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAgg                                                         <  4:272828/104‑1 (MQ=255)
                                                                                                                                    gATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAgg                                                         >  3:272828/1‑104 (MQ=255)
                                                                                                                                     aTCACCTCGTCATACAGGAAATGGCGGGCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGttt          <  3:405501/149‑1 (MQ=255)
                                                                                                                                        aCCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTaa        <  7:310740/149‑1 (MQ=255)
                                                                                                                                         ccTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAAc       <  8:231149/149‑1 (MQ=255)
                                                                                                                                          cTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACg      >  3:413102/1‑149 (MQ=255)
                                                                                                                                          cTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACg      >  1:392996/1‑149 (MQ=255)
                                                                                                                                          cTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGATACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCACCATTTTCTGTTTAACg      >  4:238029/1‑149 (MQ=255)
                                                                                                                                             gTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGAAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGctc   <  4:75723/149‑1 (MQ=255)
                                                                                                                                              tCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGctct  <  3:29355/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGGAAAAATCATCATTAAAAACAATGATGAAGCCCACCGCACACCAGAGATTCATTCCATGCTGGTGATCCCATTGTGGGAGAAAGGGGTCGTGACCGGAACGCTGAAAATTTACTACTGCCACGCGCATCAGATCACCTCGTCATTACAGGAAATGGCGGTCGGTCTGTCGCAAATCATCTCCACGCAACTGGAGGTTTCACGCGCCGAGCAGCTACGTGAAATGGCAAATAAGGCAGAGCTTCGCGCCCTGCAAAGCAAAATTAATCCCCATTTTCTGTTTAACGCTCT  >  NC_000913/2499510‑2499800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: