Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,287,422:1 +G 16.8% intergenic (‑51/‑342) acs ← / → nrfA acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,287,4221.G16.8% 831.1 / 231.1 280intergenic (‑51/‑342)acs/nrfAacetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome
Reads supporting (aligned to +/- strand):  ref base . (117/116);  new base G (21/25);  total (139/141)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 286 total aligned reads displayed.

TTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTG  >  NC_000913/4287274‑4287568
                                                                                                                                                     |                                                                                                                                                  
ttAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAAt                                                                                                                                                     >  6:35808/1‑149 (MQ=255)
    tAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGTAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATggta                                                                                                                                                <  6:94197/149‑4 (MQ=255)
    tAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAg                                                                                                                                                <  7:33226/149‑1 (MQ=255)
      gATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATggtag                                                                                                                                               <  2:9326/148‑5 (MQ=255)
      gATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAgg                                                                                                                                               <  7:303467/148‑1 (MQ=255)
              aTACATCGCCTCGTACTGCTGAGGGTTTTTCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTgg                                                                                                                                       <  1:233566/148‑1 (MQ=255)
              aTACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTgg                                                                                                                                       >  7:420583/1‑149 (MQ=255)
              aTACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTgg                                                                                                                                       >  2:245202/1‑148 (MQ=255)
                   tCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAggg                                                                                                                                              <  5:369606/136‑1 (MQ=255)
                     gCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGtttt                                                                                                                                <  8:213496/149‑1 (MQ=255)
                     gCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTg                                                                                                                               <  5:236176/149‑1 (MQ=255)
                       cgcGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGtt                                                                                                                             <  4:15122/147‑1 (MQ=255)
                       cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTa                                                                                                                                                 >  2:160913/1‑129 (MQ=255)
                       cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTa                                                                                                                                                 <  1:160913/129‑1 (MQ=255)
                       cTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGtt                                                                                                                             <  1:280516/149‑1 (MQ=255)
                          gTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTa                                                                                                                           <  1:10917/148‑1 (MQ=255)
                          gTACTGCTGAGGGTGTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCGTTTGTTCTCCTTGTATGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAg                                                                                                                          >  1:44554/1‑149 (MQ=255)
                                ctgAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTg                                                                                                                    >  8:278058/1‑149 (MQ=255)
                                  gAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGAGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTggg                                                                                                                  >  8:125504/1‑149 (MQ=255)
                                   aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAggg                                                                                                                                              <  6:376315/121‑1 (MQ=255)
                                   aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTgg                                                                                                                   <  5:70843/148‑1 (MQ=255)
                                   aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTggg                                                                                                                  <  6:410578/148‑1 (MQ=255)
                                   aGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTgg                                                                                                                   <  1:245202/147‑1 (MQ=255)
                                        ttATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGa                                                                                                               >  1:208990/1‑146 (MQ=255)
                                           tCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAt                                                                                                          <  1:422578/148‑1 (MQ=255)
                                              ggCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTGGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCAc                                                                                                       >  6:337308/1‑149 (MQ=255)
                                               gCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGc                                                                                                     <  1:98902/149‑1 (MQ=255)
                                                   cGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGAGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCaaaa                                                                                                 <  7:125504/149‑1 (MQ=255)
                                                        tGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAg                                                                                                >  5:183013/1‑146 (MQ=255)
                                                        tGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAg                                                                                             >  3:426261/1‑148 (MQ=255)
                                                          cGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAAt                                                                                          >  6:459495/1‑149 (MQ=255)
                                                                    aGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTggg                                                                                                                  >  6:20398/1‑116 (MQ=255)
                                                                        aTGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACg                                                                            >  4:323484/1‑149 (MQ=255)
                                                                           gtgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATaaa                                                                              <  3:292693/144‑1 (MQ=255)
                                                                           gtgtgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACgg                                                                           >  3:172580/1‑147 (MQ=255)
                                                                            tttgtTTGGGAATTTGGCTGATGCTTTTGTTCTCCTTGTAGGATGTTATAAATTTGTGGCATAAGCGTTATCTGTCGGGGTATTGGCCGTTTTGTTTAGTATTTGGGCGACAGATCACCCTACAGTAGAATTGTGCAAATAAACGGCAg                                                                        <  6:234781/147‑1 (MQ=255)
                                                                              ttgttGGGAATTTGGCTCATGATTTTGTGCTCCTTGTAGGATGTTAATAATATGTTGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACTCAAAAGTAGAATTGTGCAAATAAACGGCAgt                                                                       <  5:33791/146‑2 (MQ=255)
                                                                              tgtTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAgg                                                                       <  8:1933/148‑1 (MQ=255)
                                                                                tttGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGt                                                                     >  1:334176/1‑148 (MQ=255)
                                                                                       aTTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAttttt                                                              >  2:305667/1‑148 (MQ=255)
                                                                                        tttGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtt                                                                 >  7:381268/1‑144 (MQ=255)
                                                                                        tttGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAtt                                                                 <  8:381268/144‑1 (MQ=255)
                                                                                           ggCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGaa                                                           <  2:459491/148‑1 (MQ=255)
                                                                                           ggCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAgg                                                         >  6:56721/1‑149 (MQ=255)
                                                                                           ggCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAg                                                          <  4:310330/148‑1 (MQ=255)
                                                                                           ggCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAg                                                          <  4:426261/148‑1 (MQ=255)
                                                                                           ggCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAg                                                          <  8:11673/148‑1 (MQ=255)
                                                                                            gCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                        <  6:122756/149‑1 (MQ=255)
                                                                                             cTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTc                                                       <  7:48292/149‑1 (MQ=255)
                                                                                              tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAAttttt                                                              >  4:183054/1‑142 (MQ=255)
                                                                                              tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAggg                                                                                                                                              >  8:411376/1‑61 (MQ=255)
                                                                                              tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                        >  6:291584/1‑147 (MQ=255)
                                                                                              tCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGt                                                        >  5:252166/1‑147 (MQ=255)
                                                                                               cATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAg                                                     >  3:137081/1‑149 (MQ=255)
                                                                                                aTGCTTTTGTTCTCCTTGTTGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAg                                                     <  1:489686/149‑1 (MQ=255)
                                                                                                aTGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGAt                                                                                                          <  5:116810/95‑1 (MQ=255)
                                                                                                    ttttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATAACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaa                                                  >  1:119761/1‑148 (MQ=255)
                                                                                                    ttttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTTGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaac                                                 >  5:436789/1‑148 (MQ=255)
                                                                                                    ttttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaac                                                 >  7:18707/1‑148 (MQ=255)
                                                                                                    ttttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGAAGTTTTGTTTATTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATATACGGCAGGGTAATATTTGAAGGTCAGcaac                                                 >  5:48818/1‑148 (MQ=255)
                                                                                                     tttGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGcaaca                                                >  5:454616/1‑148 (MQ=255)
                                                                                                        gTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACaaaa                                             <  8:466970/148‑1 (MQ=255)
                                                                                                           ctgcTTGTAGGATGTTATTAATATGTGGCATAAGCGATAATTGTAGTGGTATTGGCAGTTTTGTTTAGTATTTGTGCGTCAGATCACGCAATAGTATAATTGTGCAAATTAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGtt                                          <  5:304031/145‑1 (MQ=255)
                                                                                                           ctcCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGtt                                          >  6:74484/1‑149 (MQ=255)
                                                                                                           ctcCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGtt                                          >  6:132258/1‑148 (MQ=255)
                                                                                                            tcCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTTGTATTTGGTCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGa                                        <  6:436789/149‑1 (MQ=255)
                                                                                                              cTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                       <  4:282848/148‑1 (MQ=255)
                                                                                                               ttGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                       >  5:211328/1‑148 (MQ=255)
                                                                                                               ttGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACa                                                                                                             <  7:129972/77‑1 (MQ=255)
                                                                                                                 gTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGc                                                                                     >  4:134412/1‑99 (MQ=255)
                                                                                                                  tAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGAt                                       >  7:200666/1‑144 (MQ=255)
                                                                                                                     gATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTTGGCGACAGAGCACGCAAAAGTAGAATTGTTCAAATAAACGGAAGGGTAATTTTTGAAGTTCAGCAACAAAAGTTGATTAATTCtt                               >  2:8624/1‑149 (MQ=255)
                                                                                                                     gATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGTGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCtt                               >  3:57602/1‑149 (MQ=255)
                                                                                                                     gATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCtt                               >  2:406875/1‑149 (MQ=255)
                                                                                                                      aTGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                              <  2:207596/149‑1 (MQ=255)
                                                                                                                        gTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGATAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                              >  7:465147/1‑147 (MQ=255)
                                                                                                                        gTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATtgtg                                                                                      >  1:357350/1‑91 (MQ=255)
                                                                                                                         ttAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGa                            <  7:265743/148‑1 (MQ=255)
                                                                                                                         ttAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGa                            <  3:323484/148‑1 (MQ=255)
                                                                                                                         ttAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAg                           <  6:266238/149‑1 (MQ=255)
                                                                                                                          taataaTATGTGGCATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTc                                                       >  2:33956/1‑121 (MQ=255)
                                                                                                                          taataaTATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAg                           >  6:250192/1‑148 (MQ=255)
                                                                                                                              aataTGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTa                                                                                              >  3:203931/1‑77 (MQ=255)
                                                                                                                               atatGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACAt                     <  8:327304/149‑1 (MQ=255)
                                                                                                                                tatGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGa                                        >  8:8944/1‑129 (MQ=255)
                                                                                                                                tatGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGa                                        <  7:8944/129‑1 (MQ=255)
                                                                                                                                     ggCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTc                                                       >  8:178209/1‑109 (MQ=255)
                                                                                                                                      gCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTa              <  5:377098/149‑1 (MQ=255)
                                                                                                                                       cATAAGCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTa              <  7:225091/149‑1 (MQ=255)
                                                                                                                                       cATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat             <  8:440444/149‑1 (MQ=255)
                                                                                                                                        aTAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatg            >  8:329985/1‑149 (MQ=255)
                                                                                                                                        aTAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat             <  5:159870/148‑1 (MQ=255)
                                                                                                                                        aTAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat             >  6:159870/1‑148 (MQ=255)
                                                                                                                                          aaGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgca          >  5:82540/1‑149 (MQ=255)
                                                                                                                                          aaGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTat             >  4:441401/1‑146 (MQ=255)
                                                                                                                                            gCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                              <  7:53942/128‑1 (MQ=255)
                                                                                                                                            gCGTTAAATGGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCttt                              >  8:53942/1‑128 (MQ=255)
                                                                                                                                              gTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCt      >  2:127025/1‑149 (MQ=255)
                                                                                                                                               ttAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTatgca          <  8:238430/144‑1 (MQ=255)
                                                                                                                                                  aaTGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAAAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGtt   >  3:371465/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTAATAGATTGTTGATACATCGCCTCGTACTGCTGAGGGTTTATCAGGCAACGGTCTGCGATGTTGGCAGGAATGGTGTGTTTGTGAATTTGGCTCATGCTTTTGTTCTCCTTGTAGGATGTTAATAATATGTGGCATAAGCGTTAAATGTAGGGGTATTGGCAGTTTTGTTTAGTATTTGGGCGACAGATCACGCAAAAGTAGAATTGTGCAAATAAACGGCAGGGTAATTTTTGAAGGTCAGCAACAAAAGTTGATTAATTCTTTGAGGAACATGCAGTTATGCATGCTGTTG  >  NC_000913/4287274‑4287568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: