Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,282,833 G→A 5.1% intergenic (+32/+107) rplY → / ← yejK 50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,282,8330GA 5.1% 371.9 / 5.8 117intergenic (+32/+107)rplY/yejK50S ribosomal subunit protein L25/spermidine nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (50/61);  new base A (3/3);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 128 total aligned reads displayed.

TACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCA  >  NC_000913/2282685‑2282980
                                                                                                                                                    |                                                                                                                                                   
tACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg                                                                                                                                                     >  4:62090/1‑149 (MQ=255)
tACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg                                                                                                                                                     >  8:209266/1‑149 (MQ=255)
tACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcg                                                                                                                                                     >  2:101472/1‑149 (MQ=255)
     cGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                                 >  8:503809/1‑148 (MQ=255)
     cGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                                 <  7:503809/148‑1 (MQ=255)
          ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttt                                                                                                                                           >  1:158794/1‑149 (MQ=255)
          ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttt                                                                                                                                           <  1:228855/149‑1 (MQ=255)
          ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttt                                                                                                                                           <  7:209266/149‑1 (MQ=255)
          ttCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtttt                                                                                                                                           <  2:118178/149‑1 (MQ=255)
            cTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGttttt                                                                                                                                          <  5:116715/148‑1 (MQ=255)
            cTGACCATCGTTGTTGACGGTAAAGAAATCAAAGGTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttt                                                                                                                                         >  2:477013/1‑149 (MQ=255)
               aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                                 <  1:208510/138‑1 (MQ=255)
               aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCcggcgg                                                                                                                                                 >  2:208510/1‑138 (MQ=255)
               aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttt                                                                                                                                         <  7:130712/146‑1 (MQ=255)
               aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGtttttt                                                                                                                                         <  2:436821/146‑1 (MQ=255)
               aCCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTa                                                                                                                                      <  7:251832/149‑1 (MQ=255)
                   tCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTg                                                                                                                                  <  5:355461/149‑1 (MQ=255)
                     gtttttGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCa                                                                                                                                <  6:35502/149‑1 (MQ=255)
                         ttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCTCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAtt                                                                                                                            <  1:238467/149‑1 (MQ=255)
                         ttgACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGAt                                                                                                                             >  7:73462/1‑148 (MQ=255)
                              ggTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGcc                                                                                                                       >  3:301384/1‑149 (MQ=255)
                               gTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCt                                                                                                                      >  4:239732/1‑149 (MQ=255)
                                tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGa                                                                                                                              <  8:344043/140‑1 (MQ=255)
                                tAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGAGCTTAATTGCTGAATAAGTTGTAAAAAACCACGCTCCGGCGGGGTTTTTTGTATCTGCAGa                                                                                                                              >  7:344043/1‑140 (MQ=255)
                                  aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGcc                                                                                                                       <  8:142738/145‑1 (MQ=255)
                                  aaGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGcc                                                                                                                       >  7:142738/1‑145 (MQ=255)
                                      aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACg                                                                                                               <  5:18015/149‑1 (MQ=255)
                                      aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACg                                                                                                               <  3:319728/149‑1 (MQ=255)
                                      aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACg                                                                                                               <  5:24444/149‑1 (MQ=255)
                                      aaTCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCGCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACg                                                                                                               <  5:19950/149‑1 (MQ=255)
                                        tCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACgg                                                                                                              >  3:268300/1‑148 (MQ=255)
                                         cAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTa                                                                                                                 <  2:450582/144‑1 (MQ=255)
                                           aaGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTAtt                                                                                                          >  1:300847/1‑149 (MQ=255)
                                            aGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATTGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTg                                                                                                         >  4:148113/1‑149 (MQ=255)
                                                 aaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtt                                                                                                    >  5:357251/1‑149 (MQ=255)
                                                 aaGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAt                                                                                                     <  3:239732/148‑1 (MQ=255)
                                                  aGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAttt                                                                                                   <  1:101472/149‑1 (MQ=255)
                                                       aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                                <  3:62090/147‑1 (MQ=255)
                                                       aGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAtttttt                                                                                                <  3:268234/147‑1 (MQ=255)
                                                        ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTg                                                                                               >  2:416465/1‑147 (MQ=255)
                                                        ggACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCa                                                                                             <  3:95505/149‑1 (MQ=255)
                                                               cAGCGTCACCCGTACCAACCGCCGCGGCAGCACAGCGCCTGCGTTCGCGCTTAATTGCTGAATACGTTGTCACCAACCCAGCGCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGa                                                                                      <  5:53822/149‑1 (MQ=255)
                                                                aGCGTCACCCCTACAAACCGAAGCTGCAGCACATCCACTTCCTTAGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCATGATTACGGTATTGCTTTTATAAGCAGGACAGa                                                                                      >  4:415297/1‑148 (MQ=255)
                                                                   gTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAg                                                                                  <  7:454361/149‑1 (MQ=255)
                                                                      aCCCGTACAAACGGAAGCTGCAGCACATCGTCTTCGTTCGCGGTTTATTGCTGTATAAGTTGTAATAAACCCCGGTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTTTTGCTATTTTTTGGAGGCCAGATAAGGc                                                                                <  3:415297/148‑1 (MQ=255)
                                                                      aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                            >  7:382106/1‑120 (MQ=255)
                                                                      aCCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTa                                                                                                            <  8:382106/120‑1 (MQ=255)
                                                                          gTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCCGGAGCGGGGtttttt                                                                                                                                         >  7:130159/1‑87 (MQ=38)
                                                                           tACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAc                                                                          >  1:80737/1‑149 (MQ=255)
                                                                              aacccGAAGCGGCAGCACATCGACTTCGTTCGCGCGTCATTGCGGAATACGTTGTAAAAACCCCCGCGCCGGCGGGGTTTTTTGTATCTGCAGAGTATGCCGGATTACGGTATTGCTATGTTTTGCAGGCCAGATACGGCGTTCAcgcc                                                                       <  1:116810/146‑1 (MQ=255)
                                                                              aaaCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                       >  7:348449/1‑149 (MQ=255)
                                                                                aCCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAg                                                                                            >  4:282541/1‑126 (MQ=255)
                                                                                 ccGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCa                                                                    >  3:30002/1‑149 (MQ=255)
                                                                                  cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCAt                                                                   >  8:274867/1‑149 (MQ=255)
                                                                                  cGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCAGCTCCGGCGGGGTTTTTTGTATCTGCAGATAATGACTGATTACGGTATTGCTAttt                                                                                                   >  6:115920/1‑117 (MQ=255)
                                                                                  agAAGCTGCAGCACATCGACTTCGTTCGAGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGAGGGTTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTAttt                                                                                                   <  5:115920/116‑1 (MQ=255)
                                                                                     aGCTGCAGCACATGGACTTCGTTCGCGATTAATTGCTGAATAAGTTGTAAAAAACCCAGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATGCGGCGTGAACGCCTTATCTg                                                                <  6:368149/149‑1 (MQ=255)
                                                                                       cTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTgg                                                               >  1:29408/1‑148 (MQ=255)
                                                                                          cagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc                                                                                <  2:199905/128‑1 (MQ=255)
                                                                                          cagcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGc                                                                                >  1:199905/1‑128 (MQ=255)
                                                                                           agcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTc                                                          <  4:30002/149‑1 (MQ=255)
                                                                                            gcaCATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCAtt                                                           >  6:217308/1‑147 (MQ=255)
                                                                                               caTCGTATTCGTTCGGGCATAAATGCCGAATTAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                      <  1:489698/149‑1 (MQ=39)
                                                                                               caTCGACTTCGTTCGCGCTTAATTGCTGAATAATTTTTAAAAACCCCCGCGCGGCCGGGGTTTTTTGTAAATGATGATTTTGCGTGATTAGGGTTTTGGTTTTTTTTGAAGGCAAGTTAGGGCGTTACCCCCGCAGGTGGcttgagaa                                                       >  2:489698/1‑141 (MQ=255)
                                                                                               caTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                       >  7:234255/1‑132 (MQ=255)
                                                                                                aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGaa                                                      >  4:96958/1‑148 (MQ=255)
                                                                                                aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATCCCTGATAAAGGTATTGAAATTTTTTGCAGGCCAGAAAAGGCGTTCACGCCGCAACTGGAATACAGaa                                                      >  3:421590/1‑148 (MQ=255)
                                                                                                aTCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCAGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAac                                                     >  6:398953/1‑149 (MQ=255)
                                                                                                 gagACTTCGTTCGCGCTTACTTGCTGAATAAGTTGTAAAACACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATACGGCGTTCACGCCGCATCTGGCATTCAGAac                                                     <  1:124992/146‑1 (MQ=255)
                                                                                                        cgttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACaaaa                                               <  4:301384/147‑1 (MQ=255)
                                                                                                          ttcgCGCTTAATTGCTGAATACGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCCGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa                                           <  2:199187/149‑1 (MQ=255)
                                                                                                          ttcgCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGa                                           <  8:249638/149‑1 (MQ=255)
                                                                                                             gcgcTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAtt                                         >  8:306907/1‑148 (MQ=255)
                                                                                                                   aaTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgc                                   <  4:268300/148‑1 (MQ=255)
                                                                                                                    aTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGTGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgcc                                  <  1:416465/148‑1 (MQ=255)
                                                                                                                    aTTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgcc                                  <  8:215187/148‑1 (MQ=255)
                                                                                                                     ttGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTgccgc                                <  2:300847/149‑1 (MQ=255)
                                                                                                                       gCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCAcgcc                                                                       <  6:60569/108‑1 (MQ=255)
                                                                                                                         tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAAtt                                     <  5:117094/140‑1 (MQ=255)
                                                                                                                         tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAAtt                                     >  6:117094/1‑140 (MQ=255)
                                                                                                                         tGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGa                            >  6:300329/1‑149 (MQ=255)
                                                                                                                          gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCa                                                                                             <  4:460354/83‑1 (MQ=255)
                                                                                                                          gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAt                                          >  7:385738/1‑134 (MQ=255)
                                                                                                                          gAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGAt                                          <  8:385738/134‑1 (MQ=255)
                                                                                                                               aGTTGTAAAAACCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCg                      <  7:417153/149‑1 (MQ=255)
                                                                                                                               aGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCg                      <  5:217308/149‑1 (MQ=255)
                                                                                                                                 ttGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgc                    >  7:11945/1‑149 (MQ=255)
                                                                                                                                   gTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGgcgc                  >  2:397494/1‑149 (MQ=255)
                                                                                                                                    tAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCATGATTACGGTATTGATATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCt                 >  4:263991/1‑149 (MQ=255)
                                                                                                                                     aaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                >  3:395789/1‑149 (MQ=255)
                                                                                                                                     aaaaaaCCCCGCCGGAGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                >  3:308300/1‑149 (MQ=255)
                                                                                                                                      aaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                >  6:23984/1‑148 (MQ=255)
                                                                                                                                      aaaaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                >  4:165589/1‑148 (MQ=255)
                                                                                                                                        aaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTg                <  8:126352/146‑1 (MQ=255)
                                                                                                                                        aaaCCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa             <  5:300329/149‑1 (MQ=255)
                                                                                                                                             ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAGGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGt        >  3:56154/1‑149 (MQ=255)
                                                                                                                                             ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa             >  6:433221/1‑144 (MQ=255)
                                                                                                                                             ccGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCaa             <  5:433221/144‑1 (MQ=255)
                                                                                                                                              cGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTc       >  6:192192/1‑149 (MQ=255)
                                                                                                                                                    ggcggGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCa  >  3:160195/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACAGCGAAGTTCTGACCATCGTTGTTGACGGTAAAGAAATCAAAGTTAAAGCTCAGGACGTACAGCGTCACCCGTACAAACCGAAGCTGCAGCACATCGACTTCGTTCGCGCTTAATTGCTGAATAAGTTGTAAAAAACCCCGCTCCGGCGGGGTTTTTTGTATCTGCAGATTATGCCTGATTACGGTATTGCTATTTTTTGCAGGCCAGATAAGGCGTTCACGCCGCATCTGGCATTCAGAACACAAAACCGATTAATTGCCGCCAGACGTCCGGCGCTGCAATTGGTCGCGCA  >  NC_000913/2282685‑2282980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: