Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,352,894 G→C 46.5% R83P (CGT→CCT)  glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,352,8940GC46.5% 10.5 / 117.5 71R83P (CGT→CCT) glpAanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
Reads supporting (aligned to +/- strand):  ref base G (20/18);  new base C (16/17);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 8.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

GTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCA  >  NC_000913/2352746‑2353028
                                                                                                                                                    |                                                                                                                                      
gTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACg                                                                                                                                        <  8:269128/149‑1 (MQ=255)
     tttGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACt                                                                                                                                   >  8:292844/1‑149 (MQ=255)
                  cACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                                                                                                                      >  5:132384/1‑149 (MQ=255)
                   aCGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGaaccaac                                                                                                                      >  6:217815/1‑148 (MQ=255)
                     gACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAAccaaccaa                                                                                                                   >  4:185688/1‑149 (MQ=255)
                       cATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACg                                                                                                                 <  2:474901/149‑1 (MQ=255)
                         tCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGc                                                                                                               <  4:182939/149‑1 (MQ=255)
                               ccGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCAGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGttt                                                                                                         <  4:17793/149‑1 (MQ=255)
                                       aCCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCt                                                                                                 >  8:428183/1‑149 (MQ=255)
                                                 aCCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGa                                                                                        <  3:431797/148‑1 (MQ=255)
                                                 aCCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGAt                                                                                       <  6:221368/149‑1 (MQ=255)
                                                  ccACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAACCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGAt                                                                                       >  7:472647/1‑148 (MQ=255)
                                                  ccACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGAt                                                                                       >  8:174210/1‑148 (MQ=255)
                                                   cACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGt                                                                                                           <  4:207122/127‑1 (MQ=255)
                                                   cACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGt                                                                                                           >  3:207122/1‑127 (MQ=255)
                                                    aCGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGAc                                                                                    >  1:48511/1‑149 (MQ=255)
                                                       gCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACctc                                                                                 <  8:472647/149‑1 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCgg                                                                                            >  1:155720/1‑135 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCgg                                                                                            <  2:155720/135‑1 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTa                                                                                                        <  5:264427/123‑1 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTa                                                                                                        >  6:264427/1‑123 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTa                                                                                                        >  6:271406/1‑123 (MQ=255)
                                                          tgctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTa                                                                                                        <  5:271406/123‑1 (MQ=255)
                                                            ctgcACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCt                                                                             <  1:88311/148‑1 (MQ=255)
                                                               cacaGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTc                                                                           >  2:53970/1‑147 (MQ=255)
                                                               cacaGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTc                                                                           >  4:10654/1‑147 (MQ=255)
                                                               cacaGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCa                                                                         <  2:48511/149‑1 (MQ=255)
                                                               cacaGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTc                                                                           >  7:19377/1‑147 (MQ=255)
                                                               cacaGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTc                                                                           <  8:19377/147‑1 (MQ=255)
                                                                            gcTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTAtt                                                            >  8:254178/1‑149 (MQ=255)
                                                                             cTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTc                                                           <  7:292844/149‑1 (MQ=255)
                                                                             cTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTc                                                           <  7:428183/149‑1 (MQ=255)
                                                                              tATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTcg                                                          >  8:305071/1‑149 (MQ=255)
                                                                                tGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTcgc                                                         <  7:254178/148‑1 (MQ=255)
                                                                                   ggTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGt                                                                                                                               >  6:423308/1‑75 (MQ=255)
                                                                                   ggTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGt                                                                                                                               <  5:423308/75‑1 (MQ=255)
                                                                                        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACg                                                                                                                 <  7:401527/84‑1 (MQ=255)
                                                                                        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACg                                                                                                                 >  8:401527/1‑84 (MQ=255)
                                                                                        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGAc                                                                                    <  3:227807/113‑1 (MQ=255)
                                                                                        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGAc                                                                                    >  4:227807/1‑113 (MQ=255)
                                                                                        ccGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaa                                                <  6:132384/149‑1 (MQ=255)
                                                                                           aTGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaagaa                                             >  4:14609/1‑149 (MQ=255)
                                                                                           aTGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCgaag                                               >  2:8122/1‑147 (MQ=255)
                                                                                                  aTCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGa                                      <  1:53970/149‑1 (MQ=255)
                                                                                                      gCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                  >  1:281262/1‑149 (MQ=255)
                                                                                                        ccGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAt                                     <  1:76288/144‑1 (MQ=255)
                                                                                                        ccGCGAAGGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTGCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGAt                                     >  2:76288/1‑144 (MQ=255)
                                                                                                          gcgAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTATATTCGCGCCTGCGAAGAAGCAGGGATCAgag                                >  4:321107/1‑145 (MQ=255)
                                                                                                             aaTGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGaa                           <  3:185688/149‑1 (MQ=255)
                                                                                                                gCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGc                         >  4:353522/1‑148 (MQ=255)
                                                                                                                gCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                  <  6:324311/139‑1 (MQ=255)
                                                                                                                gCATTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAg                                  >  5:324311/1‑139 (MQ=255)
                                                                                                                 gaTTAGGGAACACCAGATCCTGAACCGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCGGTTTATCACACTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCta                       <  4:264457/148‑1 (MQ=255)
                                                                                                                  aTTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCAc                                                                   >  6:480941/1‑104 (MQ=255)
                                                                                                                  aTTAGTGAAAACCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCAc                                                                   <  5:480941/104‑1 (MQ=255)
                                                                                                                      gTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtat                      <  3:407237/145‑1 (MQ=255)
                                                                                                                      gTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtat                      >  4:407237/1‑145 (MQ=255)
                                                                                                                         aaaaCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCg               >  6:251316/1‑149 (MQ=255)
                                                                                                                          aaaCCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgc              <  5:251316/149‑1 (MQ=255)
                                                                                                                           aaCCAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgca             <  2:281262/149‑1 (MQ=255)
                                                                                                                             ccAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgcag            >  5:305684/1‑148 (MQ=255)
                                                                                                                             ccAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTc                                                           >  4:163737/1‑101 (MQ=255)
                                                                                                                             ccAGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTc                                                           <  3:163737/101‑1 (MQ=255)
                                                                                                                               aGATCCTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCgcagc           >  6:161708/1‑147 (MQ=255)
                                                                                                                                    cTGAAACGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgc     <  7:174210/148‑1 (MQ=255)
                                                                                                                                     tGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcg    >  7:20684/1‑148 (MQ=255)
                                                                                                                                       aaaCGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAgcgcgc   <  3:14609/147‑1 (MQ=255)
                                                                                                                                       aaaCGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCa  <  5:217815/148‑1 (MQ=255)
                                                                                                                                       aaaCGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtata                     >  6:485712/1‑129 (MQ=255)
                                                                                                                                       aaaCGCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCtata                     <  5:485712/129‑1 (MQ=255)
                                                                                                                                           gCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTcgcg                                                        >  7:383054/1‑90 (MQ=255)
                                                                                                                                           gCATTGCACCTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTcgcg                                                        <  8:383054/90‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                      
GTGATTTTGGTTGAGCGCCACGACATCGCAACCGGTGCCACCGGGCGTAACCACGGCCTGCTGCACAGCGGTGCGCGCTATGCGGTAACCGATGCGGAATCGGCCCGCGAATGCATTAGTGAAAACCAGATCCTGAAACGCATTGCACGTCACTGCGTTGAACCAACCAACGGCCTGTTTATCACCCTGCCGGAAGATGACCTCTCCTTCCAGGCCACTTTTATTCGCGCCTGCGAAGAAGCAGGGATCAGCGCAGAAGCTATAGACCCGCAGCAAGCGCGCA  >  NC_000913/2352746‑2353028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: