Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,762,222 A→C 5.4% I440S (ATC→AGC)  yfjK ← radiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,762,2220AC 5.4% 393.5 / 8.5 123I440S (ATC→AGC) yfjKradiation resistance protein; DEAD/H helicase‑like protein; CP4‑57 putative defective prophage
Reads supporting (aligned to +/- strand):  ref base A (63/53);  new base C (3/4);  total (66/57)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 123 total aligned reads displayed.

TCATCAAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTGGAGG  >  NC_000913/2762075‑2762369
                                                                                                                                                   |                                                                                                                                                   
tcatcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAt                                                                                                                                                    >  2:413014/1‑149 (MQ=255)
  atcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAttt                                                                                                                                                  >  3:93331/1‑149 (MQ=255)
   tcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAtttt                                                                                                                                                 <  5:301913/149‑1 (MQ=255)
   tcaAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAtttt                                                                                                                                                 <  3:130921/149‑1 (MQ=255)
            tCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGAttt                                                                                                                                                  >  7:346018/1‑139 (MQ=255)
            tCCTGGGAAAACTCGGACAAGGGGTCTGGTTGAACGCTGGCTAACAGGGTGATTGGGGGATCAATGCCCGGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGaa                                                                                                                                        <  3:271527/149‑1 (MQ=255)
             ccTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAATCTTTTGcctgga                                                                                                                                          >  5:44883/1‑142 (MQ=255)
             ccTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAATCTTTTGcctgga                                                                                                                                          <  6:44883/146‑5 (MQ=255)
              cTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGt                                                                                                                                      <  4:410636/149‑1 (MQ=255)
                        tCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGt                                                                                                                                      >  7:77456/1‑139 (MQ=255)
                          ggACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATgc                                                                                                                          <  4:70632/149‑1 (MQ=255)
                                gTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCgg                                                                                                                    >  2:325418/1‑149 (MQ=255)
                                gTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCgg                                                                                                                    >  6:292174/1‑149 (MQ=255)
                                   gTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGgcgc                                                                                                                 >  1:298008/1‑149 (MQ=255)
                                      tGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAATCTTTTGcctgga                                                                                                                                          >  6:486465/1‑117 (MQ=255)
                                        gTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGAc                                                                                                              <  1:325418/147‑1 (MQ=255)
                                         ttGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCtt                                                                                                           >  8:432824/1‑149 (MQ=255)
                                                cTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGtt                                                                                                    >  8:367963/1‑149 (MQ=255)
                                                 tGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTc                                                                                                   <  2:265283/149‑1 (MQ=255)
                                                     tAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGACTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCATTCGCCCGGCGCGACCGTTTATTGTACCATg                                                                                              >  4:89227/1‑149 (MQ=255)
                                                       aCAGGTTTATTGGGTTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGTCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATgg                                                                                             <  3:109638/149‑1 (MQ=255)
                                                       aCAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGTATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATg                                                                                              <  7:1542/148‑1 (MQ=255)
                                                       aCAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATgg                                                                                             >  3:43058/1‑149 (MQ=255)
                                                        cAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATggg                                                                                            >  6:382641/1‑149 (MQ=255)
                                                         aGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGt                                                                                           <  1:413014/149‑1 (MQ=255)
                                                           gTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAATCTTTTGcctgga                                                                                                                                          >  4:398834/1‑96 (MQ=255)
                                                               aTTGGGGTATCAATGCCCTGAATACCGAGCGTAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATTCGCCCGGCGCGACCTTTGATGTTCCCATGTGTGAAttt                                                                                     <  4:209196/149‑1 (MQ=255)
                                                                ttGGGGTATCAATGCCCTGAATACCGAGCGGAATATCAACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTg                                                                                    >  1:162534/1‑149 (MQ=255)
                                                                atGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACGTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGACCGACCATGCGCCCGGCGCGACCTTTGATGTTCCCATGCGTGAAtttc                                                                                    >  3:62343/2‑148 (MQ=255)
                                                                  ggggTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGt                                                                                   <  8:226083/148‑1 (MQ=255)
                                                                   gggTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAAttt                                                                                     >  2:235679/1‑145 (MQ=255)
                                                                   gggTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTc                                                                                  <  3:5655/148‑1 (MQ=255)
                                                                       aTCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgc                                                                             >  8:196131/1‑149 (MQ=255)
                                                                        tCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgct                                                                            <  7:367963/149‑1 (MQ=255)
                                                                          aaTGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          >  8:313556/1‑149 (MQ=255)
                                                                          aaTGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          <  7:196131/149‑1 (MQ=255)
                                                                          aaTGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          >  1:432195/1‑149 (MQ=255)
                                                                            tGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          >  7:204336/1‑147 (MQ=255)
                                                                                cTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                    >  2:503116/1‑149 (MQ=255)
                                                                                cTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                    >  2:349251/1‑149 (MQ=255)
                                                                                cTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                    >  2:131721/1‑149 (MQ=255)
                                                                                cTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGAGTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTc                                                                    >  6:20255/1‑149 (MQ=255)
                                                                                        cGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCgg                                                            <  8:237211/149‑1 (MQ=255)
                                                                                          aGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGtt                                                          >  1:173288/1‑149 (MQ=255)
                                                                                              gAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTc                                                       >  3:242921/1‑148 (MQ=255)
                                                                                              gAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTc                                                       <  4:242921/148‑1 (MQ=255)
                                                                                                 tatCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAg                                                   <  2:298008/149‑1 (MQ=255)
                                                                                                    ccACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGAt                                                 >  2:78512/1‑148 (MQ=255)
                                                                                                    ccACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCACATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTATTCGTAGAt                                                 >  4:86304/1‑148 (MQ=255)
                                                                                                    acACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCACGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGAt                                                 >  5:449145/2‑148 (MQ=255)
                                                                                                     cACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATc                                                <  5:382641/148‑1 (MQ=255)
                                                                                                      aCCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGa                                                  >  3:380504/1‑145 (MQ=255)
                                                                                                      aCCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGa                                                  <  4:380504/145‑1 (MQ=255)
                                                                                                      aCCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATca                                               >  7:72607/1‑148 (MQ=255)
                                                                                                          ctTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACg                                          >  5:276805/1‑149 (MQ=255)
                                                                                                           ttgattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          <  2:105130/116‑1 (MQ=255)
                                                                                                           ttgattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgctgc                                                                          >  1:105130/1‑116 (MQ=255)
                                                                                                           ttgattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAAc                                           <  8:485396/147‑1 (MQ=255)
                                                                                                           ttgattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAAc                                           <  5:292174/147‑1 (MQ=255)
                                                                                                             gattgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGtt                                        <  1:503116/148‑1 (MQ=255)
                                                                                                              attgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCt                                      >  1:334881/1‑149 (MQ=255)
                                                                                                               ttgaGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAAc                                           >  6:41647/1‑143 (MQ=255)
                                                                                                               ttcataTTGACTTCCGTCATTACCTCAAGGCAAACGATTTTGCCCACGAAGTCACCGCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTa                                    <  3:89227/144‑1 (MQ=255)
                                                                                                                 gagaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTa                                    <  1:131721/148‑1 (MQ=255)
                                                                                                                   gaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCtt                                     <  4:354553/145‑1 (MQ=255)
                                                                                                                   gaTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCtt                                     >  3:354553/1‑145 (MQ=255)
                                                                                                                    aTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCGATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCCCAACGTTCTTAGCGa                                >  2:58461/1‑149 (MQ=255)
                                                                                                                    aTTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTa                                                    <  3:128554/129‑1 (MQ=255)
                                                                                                                    aTTGTCATCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGACCATGAGCCCGGCGAGACCTTTGATGATCCCATGGGTGAAATTGTCAATGCTGCGAGTTCCATCGCTGTAATCGTa                                                    >  4:128554/1‑129 (MQ=255)
                                                                                                                      tGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGa                                >  8:2828/1‑147 (MQ=255)
                                                                                                                       gTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAAtgct                                                                            >  1:448492/1‑102 (MQ=255)
                                                                                                                       gTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATa                              <  1:80252/148‑1 (MQ=255)
                                                                                                                          ttCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAgtgt                          >  5:45432/1‑149 (MQ=255)
                                                                                                                            ccGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAAttt                                                                                     >  3:14661/1‑88 (MQ=255)
                                                                                                                            ccGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAAttt                                                                                     <  4:14661/88‑1 (MQ=255)
                                                                                                                             cGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGa                       <  7:432824/149‑1 (MQ=255)
                                                                                                                             cGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGa                       >  2:241101/1‑149 (MQ=255)
                                                                                                                               ggATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTc                                       <  3:436389/131‑1 (MQ=255)
                                                                                                                               ggATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTc                                       >  4:436389/1‑131 (MQ=255)
                                                                                                                                gATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTc                    <  7:313556/149‑1 (MQ=255)
                                                                                                                                 aTTTCCTCCAGGCAAAAGCTTTTGCCGACGAAGGGAACTCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACATCGTTCTTAGCGATAGTGTTGACTcc                   <  8:72607/149‑1 (MQ=255)
                                                                                                                                  tttCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCt                  <  6:45432/149‑1 (MQ=255)
                                                                                                                                  tttCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCt                  <  4:93331/149‑1 (MQ=255)
                                                                                                                                  tttCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCt                  >  8:202247/1‑149 (MQ=255)
                                                                                                                                     cctccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTc                <  2:162534/148‑1 (MQ=255)
                                                                                                                                      ctccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCaa              >  3:206820/1‑149 (MQ=255)
                                                                                                                                       tccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAg                                   >  5:317735/1‑127 (MQ=255)
                                                                                                                                       tccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAg                                   <  6:317735/127‑1 (MQ=255)
                                                                                                                                       tccAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAAt             >  4:234849/1‑149 (MQ=255)
                                                                                                                                         cAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGa           <  2:334881/149‑1 (MQ=255)
                                                                                                                                            gCAAAAGATTTTGCAGACGAAGTGAACGCCCATGCTCCAGGCGAGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACCTTCTTAGAGATAGTGTTGACTCCTTAAATGATct        >  2:63193/1‑148 (MQ=255)
                                                                                                                                              aaaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTg      >  1:457799/1‑149 (MQ=255)
                                                                                                                                               aaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGa                                                  >  8:170771/1‑104 (MQ=255)
                                                                                                                                               aaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGa                                                  <  7:170771/104‑1 (MQ=255)
                                                                                                                                               aaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTgg     >  5:441119/1‑149 (MQ=255)
                                                                                                                                               aaaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCAATGGGTGAATTTGTCAATGCTGAGAGTTCCATAGCGCTTCTCGTAGAt                                                 >  4:254651/1‑105 (MQ=255)
                                                                                                                                                aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCAGAACGTTCTTAGCGATAGTGTTGACTCGTTCAATGATCGTGGa    <  6:449145/149‑1 (MQ=255)
                                                                                                                                                aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATtgtg      >  1:185502/1‑147 (MQ=255)
                                                                                                                                                aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTg      >  2:259401/1‑147 (MQ=255)
                                                                                                                                                aaGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTGGa    <  1:349251/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCATCAAAGCGATCCTGTGAAAACTCGGACAAGTGGTCTGGTTGAACGCTGGCTAACAGGTTGATTGGGGTATCAATGCCCTGAATACCGAGCGGAATATCCACCTCTTGATTGAGATTGTCTTCCGGGATTTCCTCCAGGCAAAAGATTTTGCCGACGAAGTGAACGCCCATGCGCCCGGCGCGACCTTTGATGTTCCCATGGGTGAATTTGTCAATGCTGCGAGTTCCATCGCGGTTGTCGTAGATCACAACGTTCTTAGCGATAGTGTTGACTCCTTCAATGATCGTGGAGG  >  NC_000913/2762075‑2762369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: