Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,791,077 C→A 6.5% P366T (CCG→ACG)  lhgO → L‑2‑hydroxyglutarate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,791,0770CA 6.5% 330.7 / 2.1 99P366T (CCG→ACG) lhgOL‑2‑hydroxyglutarate oxidase
Reads supporting (aligned to +/- strand):  ref base C (49/42);  new base A (2/6);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

GCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGC  >  NC_000913/2790929‑2791225
                                                                                                                                                    |                                                                                                                                                    
gCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGAATGACGATTTTCTGTTTGTCACCAccc                                                                                                                                                      >  1:148971/1‑149 (MQ=255)
 cTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGAGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCAcccc                                                                                                                                                     >  6:395587/1‑149 (MQ=255)
  tgtgCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCcg                                                                                                                                                    >  4:356524/1‑149 (MQ=255)
    tgCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCcgcg                                                                                                                                                  >  2:2194/1‑149 (MQ=255)
         aaGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGa                                                                                                                                             <  5:395587/149‑1 (MQ=255)
         aaGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGa                                                                                                                                             >  4:445205/1‑149 (MQ=255)
           gCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATc                                                                                                                                           <  3:35539/149‑1 (MQ=255)
               gtATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGGGATTTCCAGGCCTGGCCGGTGGGTGTGGGGGCGCAGGCGGTTTGGCCGGACGGGAAGCTGATTGATGATTTTCTGTTTGTGACCTCGCCGCGCTCGATCcaca                                                                                                                                       <  2:148971/148‑1 (MQ=255)
                             tGCAAAAGTATTGTCCCCGGATTTAGTAAAGCGATCTCCACACCAGCCCCGCCGGTGAGCGGGCGCAGGCGGTATCGCCGGAAGGAAAGCTGATTGACTATTTTATCTTTGACACCACCACGCGCACGATCCACACCTGCAATGCTcc                                                                                                                          >  4:347605/1‑148 (MQ=255)
                               cAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTc                                                                                                                       >  4:53367/1‑149 (MQ=255)
                                aaaaGTATTGTCCCCGGCTTTCGTTACGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGGATCGCCGGCCGGCAAGCTGATTGACGATGTTCTGTGTGTCCCCACCCCGCGCACGATCCACCCCTGCAATGCGCCCTca                                                                                                                      >  1:224317/1‑148 (MQ=255)
                                aaaaGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTcc                                                                                                                      >  2:249344/1‑149 (MQ=255)
                                aaaaGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTcc                                                                                                                      >  4:349546/1‑149 (MQ=255)
                                aaaaGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTcc                                                                                                                      >  5:451682/1‑149 (MQ=255)
                                aaaaGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACATGCAATGCGCCCtac                                                                                                                      >  2:129602/1‑147 (MQ=255)
                                  aaGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGAGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGTTTTTATGTTTGTCACCTCGACGAGCACGATCAACACCTGCAATGCGCCCTcccc                                                                                                                    <  8:399674/149‑1 (MQ=255)
                                       ttGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAg                                                                                                               >  3:278135/1‑149 (MQ=255)
                                          tCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGa                                                                                                            >  3:176690/1‑149 (MQ=255)
                                             ccGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACa                                                                                                          >  3:457929/1‑148 (MQ=255)
                                             ccGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACAt                                                                                                         <  7:69603/149‑1 (MQ=255)
                                              cGGCTTTCGTTAAGCGTTCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCTCGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATc                                                                                                        <  4:278135/149‑1 (MQ=255)
                                              cGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCGCCCCCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACAt                                                                                                         >  5:119886/1‑148 (MQ=255)
                                               ggCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCTGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCa                                                                                                       <  5:353246/149‑1 (MQ=255)
                                               ggCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGa                                                                                                            >  2:201092/1‑144 (MQ=255)
                                               ggCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGa                                                                                                            <  1:201092/144‑1 (MQ=255)
                                                   ttCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCaa                                                                                                   >  1:348435/1‑149 (MQ=255)
                                                      gTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTc                                                                                                >  7:487578/1‑149 (MQ=255)
                                                       ttAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTcc                                                                                               >  2:120125/1‑149 (MQ=255)
                                                       ttAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTcc                                                                                               >  4:308747/1‑149 (MQ=255)
                                                        tAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACAcc                                                                                                                                     <  7:417808/110‑1 (MQ=255)
                                                        tAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACAcc                                                                                                                                     >  8:417808/1‑110 (MQ=255)
                                                          aGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTCTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAt                                                                                            <  1:2194/149‑1 (MQ=255)
                                                           gCGATCTCCAGCCCTGGCCCTCCGGTGTGAGGGCGCAGGCGGTATCGCCGGACGGCTAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAtt                                                                                           <  3:347605/149‑1 (MQ=255)
                                                           gCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAtt                                                                                           >  2:276707/1‑149 (MQ=255)
                                                           gCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAtt                                                                                           <  3:445205/149‑1 (MQ=255)
                                                            cGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCACGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTg                                                                                          <  2:297100/149‑1 (MQ=255)
                                                                   cAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGt                                                                                        <  3:356524/144‑1 (MQ=255)
                                                                   cAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCa                                                                                   >  2:251399/1‑149 (MQ=255)
                                                                       ccTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATAtt                                                                               <  3:349546/149‑1 (MQ=255)
                                                                       ccTGGCCAGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACAGCAGCAATTCCTATTGGTGCGCATAtt                                                                               <  6:442530/149‑1 (MQ=255)
                                                                         tGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTGTGTCCCCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGt                                                                             >  1:130336/1‑149 (MQ=255)
                                                                           gCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCa                                                                           <  1:249344/149‑1 (MQ=255)
                                                                              cgccgGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCCCCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGc                                                                         >  7:101002/1‑148 (MQ=255)
                                                                               gccgGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCGCCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCaa                                                                       <  6:119886/149‑1 (MQ=255)
                                                                               gccgGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGc                                                                         >  4:432905/1‑147 (MQ=255)
                                                                                ccgGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGCTCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAg                                                                      >  2:205076/1‑149 (MQ=255)
                                                                                ccgGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAg                                                                      >  7:252438/1‑149 (MQ=255)
                                                                                       gCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCTCGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACaaa                                                               <  4:188582/149‑1 (MQ=255)
                                                                                        cGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAtt                                                                                           <  5:483077/120‑1 (MQ=255)
                                                                                             gcAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAAt                                                                                                  <  3:448043/108‑1 (MQ=255)
                                                                                             gcAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAt                                                                                            >  6:287788/1‑114 (MQ=255)
                                                                                             gcAGGCGGTAGCGCCGGACGGCAAGCTGATTGACGAGTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCATATTGGTGCGCATATTGGCAGCAAGGTACAAACGCTGt                                                         <  2:224317/149‑1 (MQ=255)
                                                                                              cAGGCGGTATCGCCGGACGGCAAGCTGATTGAAGATTTTCTGTTTGTTACGTCGACGAGCTAGATCTACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGtt                                                        <  2:12981/149‑1 (MQ=255)
                                                                                               aGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTg                                                       <  8:252438/149‑1 (MQ=255)
                                                                                                ggCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTgg                                                      <  5:171740/149‑1 (MQ=255)
                                                                                                     tATCGCCGGACGGCAAGCTGATTGATTTTTTTTTTTTTGTTACGTCGACGAGCTAGATCTACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGc                                                 <  4:457929/149‑1 (MQ=255)
                                                                                                      aTCGCCGGACGGCAAGCTGATTGATGTTTTTTTTTTTGTTACGTCGACGAGCTAGATCTACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCatat                                                                                <  8:325832/117‑1 (MQ=255)
                                                                                                      aTCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCatat                                                                                <  6:262610/117‑1 (MQ=255)
                                                                                                      aTCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCatat                                                                                >  7:325832/1‑117 (MQ=255)
                                                                                                      aTCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCatat                                                                                >  5:262610/1‑117 (MQ=255)
                                                                                                      aTCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGc                                                 >  2:313020/1‑148 (MQ=255)
                                                                                                         gCCGGACGGCAAGCTGATTGACTATTTTCTGTTTGTCACAACAACCCGCACGATCCACACATGCAATGATCCCTCCCCGGCAGCGACATCAGCAATTCATATTCGTGCGCATATTGTCAGAAAGGTACAAAAGCTGTTGGCAAACCa                                               >  5:139323/1‑147 (MQ=255)
                                                                                                         gCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCaga                                             <  1:120125/149‑1 (MQ=255)
                                                                                                         gCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCaga                                             <  1:251399/149‑1 (MQ=255)
                                                                                                         gCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCaga                                             <  7:15846/149‑1 (MQ=255)
                                                                                                         gCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCa                                               >  1:22685/1‑147 (MQ=255)
                                                                                                           cGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTgg                                                      >  1:347551/1‑138 (MQ=255)
                                                                                                           cGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGt                                           <  1:276707/149‑1 (MQ=255)
                                                                                                                  cAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACCCCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCg                                    <  2:130336/149‑1 (MQ=255)
                                                                                                                   aaGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGt                                                                             <  4:413683/107‑1 (MQ=255)
                                                                                                                   aaGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGt                                                                             >  3:413683/1‑107 (MQ=255)
                                                                                                                   aaGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCa                                               <  5:407431/137‑1 (MQ=255)
                                                                                                                   aaGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCa                                               >  6:407431/1‑137 (MQ=255)
                                                                                                                         aTTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGacgcac                             <  3:53367/149‑1 (MQ=255)
                                                                                                                          ttGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACCCCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGacgcacg                            >  4:407912/1‑149 (MQ=255)
                                                                                                                            gACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGacgcacgc                           <  3:432905/148‑1 (MQ=255)
                                                                                                                            gACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCt                          >  5:328744/1‑149 (MQ=255)
                                                                                                                              cGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGTTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGc                        >  1:288121/1‑149 (MQ=255)
                                                                                                                              cGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGc                        >  3:5769/1‑149 (MQ=255)
                                                                                                                               gATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATACCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGc                        >  3:180396/1‑148 (MQ=255)
                                                                                                                                 ttttCTGTTTGTCACCAACCCGCGCACGATCCCCACCTGCAATGCGCCCTCCCCGGCAGCGACCTCCGCAATTCCTATTGGTGCTCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTgaga                      <  1:129602/148‑1 (MQ=255)
                                                                                                                                    tCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGAGACAACAGCAATTCCTATTGATGCGCATATTGACAGCAAGGTACAAACGCTGTTGGCCAGCCAGAGTAACCCCGGACCCACGCTGCGAGCgg                  >  7:441297/1‑149 (MQ=255)
                                                                                                                                      tGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCa                >  7:108433/1‑149 (MQ=255)
                                                                                                                                       gTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCAc               >  1:188/1‑149 (MQ=255)
                                                                                                                                         ttGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAATCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACg              >  2:260074/1‑148 (MQ=255)
                                                                                                                                         ttGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACg              >  7:72319/1‑148 (MQ=255)
                                                                                                                                            tCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAgt          <  4:5769/149‑1 (MQ=255)
                                                                                                                                              accaccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGa                                                                                                            >  2:213132/1‑49 (MQ=255)
                                                                                                                                              accaccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGa                                                                                                            <  1:213132/49‑1 (MQ=255)
                                                                                                                                              accaccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAgtgt        <  2:348435/149‑1 (MQ=255)
                                                                                                                                                caccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTa                                                                   <  6:92939/88‑1 (MQ=255)
                                                                                                                                                caccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTa                                                                   >  5:92939/1‑88 (MQ=255)
                                                                                                                                                caccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTACCCCCGGACGCACGCTGCGAGCGGCACGTAGtgtg       >  2:377753/1‑148 (MQ=255)
                                                                                                                                                caccCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGtgtg       >  6:357347/1‑148 (MQ=255)
                                                                                                                                                 accCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAACCGCTGTTGGCAAGCCACAGTAACCCCGGACGCCCCCGGCGCGCGGCAGGTAGTGTGGa     >  6:316179/1‑149 (MQ=255)
                                                                                                                                                   cccGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCgg                  >  6:462760/1‑134 (MQ=255)
                                                                                                                                                   cccGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCgg                  <  5:462760/134‑1 (MQ=255)
                                                                                                                                                    ccGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAt                                                                                            >  2:309327/1‑59 (MQ=255)
                                                                                                                                                    ccGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTAt                                                                                            <  1:309327/59‑1 (MQ=255)
                                                                                                                                                    ccGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGc  >  5:452059/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCTGTGCAAAAGCGGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCCTGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTTCTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACATCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAGAGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGC  >  NC_000913/2790929‑2791225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: