Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,161,239 C→G 5.9% intergenic (‑93/+18) ygiQ ← / ← ftsP Radical SAM superfamily protein/septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,161,2390CG 5.9% 276.0 / 6.3 82intergenic (‑93/+18)ygiQ/ftsPRadical SAM superfamily protein/septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
Reads supporting (aligned to +/- strand):  ref base C (31/46);  new base G (3/2);  total (34/48)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TGCGGCCAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTC  >  NC_000913/3161094‑3161387
                                                                                                                                                 |                                                                                                                                                    
tGCGGCCAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCg                                                                                                                                                   >  7:464069/1‑149 (MQ=255)
  cGGCCAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCg                                                                                                                                                   <  5:425046/147‑1 (MQ=255)
    gacAGGAGAACAGGGCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCgg                                                                                                                                                  <  4:104965/144‑1 (MQ=255)
    gCCAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGCGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCgg                                                                                                                                                  >  3:104965/1‑146 (MQ=255)
     ccAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGa                                                                                                                                              <  6:61870/149‑1 (MQ=255)
     ccAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGa                                                                                                                                              <  2:326203/149‑1 (MQ=255)
      cAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAg                                                                                                                                             <  4:156270/149‑1 (MQ=255)
      cAGGAGAACAGGTCGCGATCCGGCTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAg                                                                                                                                             <  1:155509/149‑1 (MQ=255)
       aGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAg                                                                                                                                             >  3:143321/1‑148 (MQ=255)
       aGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGt                                                                                                                                            <  8:127927/149‑1 (MQ=255)
         gagaACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTAt                                                                                                                                          <  4:273289/149‑1 (MQ=255)
         gagaACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTAt                                                                                                                                          <  1:245472/149‑1 (MQ=255)
             aCAGGTCGCGATCCGGTTGGATCAGGGAGATTGAGCTCATTTTCCTTCCAGAAATGATAAAAAACTAATCAAAGGACGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAg                                                                                                                                      <  4:78740/149‑1 (MQ=255)
               aGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGAt                                                                                                                                    <  7:73059/149‑1 (MQ=255)
                  tCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGTAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTAc                                                                                                                                 <  8:227698/149‑1 (MQ=255)
                   cgcgATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACg                                                                                                                                >  2:380373/1‑149 (MQ=255)
                    gcgATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACgg                                                                                                                               <  5:51702/149‑1 (MQ=255)
                      gATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTa                                                                                                                             >  7:100660/1‑149 (MQ=255)
                           ggTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGa                                                                                                                        >  1:271036/1‑149 (MQ=255)
                            gTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTAACGGAt                                                                                                                       >  3:456237/1‑149 (MQ=255)
                             ttGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGAtt                                                                                                                      <  1:380373/149‑1 (MQ=255)
                                gATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGAc                                                                                                                   <  4:122794/149‑1 (MQ=255)
                                  tCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGCACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCGTCGGGCGAGTATGAAGATTAAGGTACCGGATTGAcc                                                                                                                  >  2:228042/1‑148 (MQ=255)
                                        agaTAGCGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGa                                                                                                                                        >  8:181322/1‑120 (MQ=255)
                                        agaTAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGa                                                                                                                                        <  7:181322/120‑1 (MQ=255)
                                              agCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCaa                                                                                                     <  2:241260/149‑1 (MQ=255)
                                              agCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCaa                                                                                                     >  3:234425/1‑149 (MQ=255)
                                               gCTCCTTTTGCGTCCAGAAATGATAAAATAATAATAAAAGGCCGGGTATTCTAACCCGGAACGAAAGAGAAAACGAAAGGTATTCCAAACTCGCCCTCCTCGGGCGAGTATGAAGAtt                                                                                                                                   <  5:282503/118‑1 (MQ=255)
                                                cTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATc                                                                                                   <  8:164445/149‑1 (MQ=255)
                                                 tCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATTAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATc                                                                                                   >  1:69390/1‑148 (MQ=255)
                                                 tCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATc                                                                                                   >  4:131067/1‑148 (MQ=255)
                                                  cATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGa                                                                                                 <  7:414139/149‑1 (MQ=255)
                                                   aTTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAg                                                                                                <  1:37659/149‑1 (MQ=255)
                                                     tttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGc                                                                                               >  1:265996/1‑148 (MQ=255)
                                                      ttGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGcc                                                                                              <  7:331126/148‑1 (MQ=255)
                                                        gCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCAc                                                                                            <  2:271036/148‑1 (MQ=255)
                                                          ttCCAGAAATGATAAAAAAATAATCAAAGGCCGTGGATTATAAGCAGTAACGAAAGAGAAATCGAAAGTTATTCCATACTCGCACTCCTCGGGCGAGTATGAAGATTACTGTACCGGATTGACCAACAGTATCCCAATCGAGCCACGGt                                                                                         >  6:345007/1‑149 (MQ=255)
                                                          ttCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGt                                                                                         >  8:413097/1‑149 (MQ=255)
                                                            ccAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTc                                                                                        <  7:433186/148‑1 (MQ=255)
                                                              aGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGc                                                                                     <  8:464069/149‑1 (MQ=255)
                                                                aaaTGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCa                                                                                   >  6:97686/1‑149 (MQ=255)
                                                                  aTGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCAtt                                                                                 >  3:399509/1‑149 (MQ=255)
                                                                    gATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGAtt                                                                                                                      <  8:30478/110‑1 (MQ=255)
                                                                      tAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTc                                                                               >  7:268545/1‑147 (MQ=255)
                                                                         aaaaaTAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCg                                                                          <  4:143321/149‑1 (MQ=255)
                                                                           aaaTAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTc                                                                        <  4:456237/149‑1 (MQ=255)
                                                                             ataatCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCt                                                                       >  6:246104/1‑148 (MQ=255)
                                                                                   aaaGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGtt                                                                >  2:231379/1‑149 (MQ=255)
                                                                                    aaGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGtt                                                                >  3:267989/1‑148 (MQ=255)
                                                                                    aaGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTg                                                               <  8:100660/149‑1 (MQ=255)
                                                                                     aGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGa                                                              <  3:131067/149‑1 (MQ=255)
                                                                                      ggCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGaa                                                             <  4:267989/149‑1 (MQ=255)
                                                                                      ggCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGaa                                                             <  5:246104/149‑1 (MQ=255)
                                                                                      gcccGGGGAATATAAGCCGGAACTAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTTACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGaa                                                             <  1:360891/147‑1 (MQ=255)
                                                                                          ggggATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAg                                                            >  8:420854/1‑146 (MQ=255)
                                                                                               ttATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACgg                                                    <  5:432559/149‑1 (MQ=255)
                                                                                               ttATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACgg                                                    <  5:97686/149‑1 (MQ=255)
                                                                                                  taAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGaa                                                 <  4:234425/149‑1 (MQ=255)
                                                                                                  taAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGaa                                                 <  2:265996/149‑1 (MQ=255)
                                                                                                           aCGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCa                                        <  1:231379/149‑1 (MQ=255)
                                                                                                             gAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCCCGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGCACGGGAAGTGCGCCCAg                                       >  8:96585/1‑148 (MQ=255)
                                                                                                               aaGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAgg                                      >  1:188515/1‑147 (MQ=255)
                                                                                                                  agaAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCGCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGc                                 <  2:69390/149‑1 (MQ=255)
                                                                                                                      aTCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGAc                             >  4:174114/1‑149 (MQ=255)
                                                                                                                       tCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGAcc                            >  3:57728/1‑149 (MQ=255)
                                                                                                                             ggTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATa                      <  7:420854/149‑1 (MQ=255)
                                                                                                                               atcttcATACTCGCCCGAGGAGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGa                                                              <  3:403634/102‑1 (MQ=37)
                                                                                                                               atcttcATACTCGCCCGAGGAGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGa                                                              >  4:403634/6‑107 (MQ=37)
                                                                                                                                 ttCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAg                                       >  6:389552/1‑128 (MQ=255)
                                                                                                                                 ttCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAg                                       <  5:389552/128‑1 (MQ=255)
                                                                                                                                 ttCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAAc                   <  2:188515/148‑1 (MQ=255)
                                                                                                                                  ttcATACTCGCCCGAGGAGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTcc                                                                              <  1:80471/86‑1 (MQ=37)
                                                                                                                                  ttcATACTCGCCCGAGGAGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTcc                                                                              >  2:80471/3‑88 (MQ=37)
                                                                                                                                   ccATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACaa                 <  8:268545/148‑1 (MQ=255)
                                                                                                                                    cATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagc               <  5:345007/149‑1 (MQ=255)
                                                                                                                                    cATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAag                >  1:24465/1‑148 (MQ=255)
                                                                                                                                       aCTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAagcagc            >  3:266130/1‑149 (MQ=255)
                                                                                                                                         tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCACCAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTc          >  7:6057/1‑149 (MQ=255)
                                                                                                                                         tCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTc          >  1:81061/1‑149 (MQ=255)
                                                                                                                                           gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCt                                >  7:453054/1‑125 (MQ=255)
                                                                                                                                           gCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCt                                <  8:453054/125‑1 (MQ=255)
                                                                                                                                            cccTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCAc       <  4:399509/149‑1 (MQ=255)
                                                                                                                                             cctcctCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACt      >  1:158369/1‑149 (MQ=255)
                                                                                                                                             catcctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGCGCCACGGACCGCCATTTCCAGCGTCTGCATGATGAAGTAGAACGGGAAGTGCGCCCAGGAATGCTGACCGAAAAACACAAGCAGCTCCACt      >  6:378125/3‑149 (MQ=255)
                                                                                                                                              ctcctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACtt     >  2:306018/1‑149 (MQ=255)
                                                                                                                                                 ctcGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTc  >  1:198185/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGCGGCCAGGAGAACAGGTCGCGATCCGGTTGGATCAGGGAGATAGAGCTCATTTTGCTTCCAGAAATGATAAAAAAATAATCAAAGGCCGGGGATTATAAGCCGGAACGAAAGAGAAATCGAAAGGTATTCCATACTCGCCCTCCTCGGGCGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGAGCCACGGTCCGCCATTTCCAGCGTCTGACTGTTGAAGTAGAACGGGAAGTGCGCCCAGGAAGGCTGACCGAAATAAACAAGCAGCTCCACTTGTC  >  NC_000913/3161094‑3161387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑