Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,276,887 T→C 6.5% intergenic (+34/‑115) garD → / → prlF D‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,276,8870TC 6.5% 370.8 / 11.3 124intergenic (+34/‑115)garD/prlFD‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (56/59);  new base C (4/4);  total (61/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.36e-01

Only 100 of 125 total aligned reads displayed.

TGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTA  >  NC_000913/3276740‑3277031
                                                                                                                                                   |                                                                                                                                                
tGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCt                                                                                                                                                  >  3:102559/1‑148 (MQ=255)
  gAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAg                                                                                                                                               <  1:338542/149‑1 (MQ=255)
    aGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTg                                                                                                                                             >  4:88805/1‑149 (MQ=255)
     gttgttACACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTATCGGATCAATGGGGGCTGCATAACCAGCTTGCGGTGTTTAACCCGGCAGCGGTGACCTGATTTCTTTTCGAACGTCCATAGCAAAACATGGCATTAGTGc                                                                                                                                            >  3:283045/1‑149 (MQ=255)
         ttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATg                                                                                                                                        >  5:64957/1‑149 (MQ=255)
            cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGCCCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGttt                                                                                                                                     >  6:260565/1‑149 (MQ=255)
            cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTa                                                                                                                                                >  2:288205/1‑138 (MQ=255)
            cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTa                                                                                                                                                <  1:288205/138‑1 (MQ=255)
             aCTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtttt                                                                                                                                    >  1:293299/1‑149 (MQ=255)
             aCTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtttt                                                                                                                                    >  7:197516/1‑149 (MQ=255)
             aCTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGtttt                                                                                                                                    <  2:95547/149‑1 (MQ=255)
              cTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCAtttttt                                                                                                                                    >  3:293049/1‑148 (MQ=255)
              cTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtttt                                                                                                                                    >  6:471680/1‑148 (MQ=255)
                 tATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                >  8:140272/1‑149 (MQ=255)
                 tATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAt                                                                                                                                 <  7:26452/148‑1 (MQ=255)
                  aTTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                <  6:218154/148‑1 (MQ=255)
                  aTTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                <  1:195020/148‑1 (MQ=255)
                   ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTg                                                                                                                               <  2:293299/148‑1 (MQ=255)
                   ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTg                                                                                                                               <  7:397950/148‑1 (MQ=255)
                    tcttGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTa                                                                                                                                  <  1:87957/144‑1 (MQ=255)
                     ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGtt                                                                                                                             >  5:214979/1‑148 (MQ=255)
                     ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGttttgcga                                                                                                                                <  3:349578/145‑5 (MQ=255)
                      tcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGttt                                                                                                                            >  5:397619/1‑148 (MQ=255)
                      tcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGAGGTTAACCCGGCACCGGTGACCGGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCATAGTGCCATGTTTTTAATGttt                                                                                                                            >  4:180263/1‑148 (MQ=255)
                        gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                           >  7:184519/1‑147 (MQ=255)
                               ccAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATTTTTTTATTGTTTAAAGCCCCCa                                                                                                                  <  4:293049/149‑1 (MQ=255)
                                  gCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGt                                                                                                               <  8:197516/149‑1 (MQ=255)
                                   cGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                              <  6:64957/149‑1 (MQ=255)
                                   cGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                              <  5:388447/149‑1 (MQ=255)
                                     ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCa                                                                                                            <  4:142936/149‑1 (MQ=255)
                                      gaagaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                           <  2:381992/149‑1 (MQ=255)
                                         gaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaa                                                                                                        <  8:470492/149‑1 (MQ=255)
                                          aagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaa                                                                                                        >  4:259780/1‑148 (MQ=255)
                                            gaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                     >  6:138410/1‑149 (MQ=255)
                                             aaaaCCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTaataat                                                                                                    <  6:109658/149‑1 (MQ=255)
                                                  cTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatt                                                                                               >  5:379166/1‑149 (MQ=255)
                                                        ggATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcatt                                                                                         <  7:140272/149‑1 (MQ=255)
                                                        ggATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcatt                                                                                         <  6:397619/149‑1 (MQ=255)
                                                            cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGAGTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCt                                                                                     >  2:121483/1‑149 (MQ=255)
                                                              aggggggCTGCATACCCAGCTGGCGGTGGTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGCCCCGCGCAAAACAGGGCCTTAGTGCCATGTTATTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                                   <  6:400509/147‑1 (MQ=255)
                                                              aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGt                                                                                                                              >  6:420706/1‑106 (MQ=255)
                                                              aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCt                                                                                     >  5:133648/1‑147 (MQ=255)
                                                              aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCt                                                                                     >  1:411814/1‑147 (MQ=255)
                                                              aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCt                                                                                     >  1:276468/1‑147 (MQ=255)
                                                              aTGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTa                                                                                   <  2:276468/149‑1 (MQ=255)
                                                                 ggggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGt                                                                                 <  8:483197/148‑1 (MQ=255)
                                                                 ggggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCGCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGt                                                                                 <  1:182477/148‑1 (MQ=255)
                                                                    gCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCaa                                                                             <  3:88805/149‑1 (MQ=255)
                                                                      tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGtt                                                                                <  1:438317/144‑1 (MQ=255)
                                                                      tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGtt                                                                                >  2:438317/1‑144 (MQ=255)
                                                                        cATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                          >  8:305929/1‑148 (MQ=255)
                                                                         aTAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTta                                                                        >  3:323534/1‑149 (MQ=255)
                                                                          tAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTtat                                                                       <  5:138410/149‑1 (MQ=255)
                                                                            aCCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTtata                                                                      <  5:230332/148‑1 (MQ=255)
                                                                             ccAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                <  7:193125/89‑1 (MQ=255)
                                                                                 cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGa                                                                <  6:379166/149‑1 (MQ=255)
                                                                                    gCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGc                                                              <  3:259780/148‑1 (MQ=255)
                                                                                    gCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCa                                                             <  8:377670/149‑1 (MQ=255)
                                                                                          tttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTta                                                                        <  1:262332/132‑1 (MQ=255)
                                                                                            tAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAg                                                     >  3:342450/1‑149 (MQ=255)
                                                                                              aCCCGGCACCTGTTACCTGATTTCTTTTGGAAAGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                    <  3:180263/148‑1 (MQ=255)
                                                                                              aCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                    <  4:102559/148‑1 (MQ=255)
                                                                                              aCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGc                                                   >  4:331412/1‑149 (MQ=255)
                                                                                               cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTg                                                                 >  1:66699/1‑134 (MQ=255)
                                                                                               cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTg                                                                 <  2:66699/134‑1 (MQ=255)
                                                                                                ccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTTAAGCCGCCACGTCCATTTATATTGCATTTGGATTACGTTAGTTCAAGCTTATAATTTGTGCTGTAAGCAGGCTg                                                 >  8:483163/1‑149 (MQ=255)
                                                                                                ccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                  <  7:483163/148‑1 (MQ=255)
                                                                                                 cGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTg                                                                 >  8:166893/1‑132 (MQ=255)
                                                                                                    cACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaa                                              >  1:80349/1‑148 (MQ=255)
                                                                                                     aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                            <  6:214979/149‑1 (MQ=255)
                                                                                                     aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                            <  5:471680/149‑1 (MQ=255)
                                                                                                     aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTaaaa                                            <  7:305929/149‑1 (MQ=255)
                                                                                                         gTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGa                                         >  6:472680/1‑148 (MQ=255)
                                                                                                          tGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                       <  2:99355/149‑1 (MQ=255)
                                                                                                            aCCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGt                                     <  5:260565/149‑1 (MQ=255)
                                                                                                              cTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTg                                    >  7:413256/1‑148 (MQ=255)
                                                                                                              atGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGATTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGAATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTg                                    >  7:354723/2‑148 (MQ=255)
                                                                                                                gATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                          <  1:60072/108‑1 (MQ=255)
                                                                                                                gATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                          >  2:60072/1‑108 (MQ=255)
                                                                                                                gATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAAt                                 >  8:324226/1‑149 (MQ=255)
                                                                                                                      ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCAt                              >  4:391506/1‑146 (MQ=255)
                                                                                                                      ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCAt                              <  3:391506/146‑1 (MQ=255)
                                                                                                                        ttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGcc                           <  8:184519/147‑1 (MQ=255)
                                                                                                                           gAACGTCCCTCGCAAAACATGGTCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTGATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCAGCt                       >  7:92704/1‑148 (MQ=255)
                                                                                                                           gAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAACACAGGCTGTAAAAGGACAGTGAATCATGCCCGCt                       <  4:253812/148‑1 (MQ=255)
                                                                                                                                tCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgct                 >  7:369276/1‑149 (MQ=255)
                                                                                                                                  ccTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcg               <  1:354846/149‑1 (MQ=255)
                                                                                                                                   cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgc              <  7:324226/149‑1 (MQ=255)
                                                                                                                                   cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgc              >  5:468263/1‑149 (MQ=255)
                                                                                                                                    aataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTaa                     >  4:66947/5‑141 (MQ=255)
                                                                                                                                    aataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTaa                     <  3:66947/137‑1 (MQ=255)
                                                                                                                                      taaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGa                                   >  7:496813/3‑125 (MQ=255)
                                                                                                                                      taaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGa                                   <  8:496813/123‑1 (MQ=255)
                                                                                                                                        aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                    >  4:369517/1‑106 (MQ=255)
                                                                                                                                        aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAgg                                                    <  3:369517/106‑1 (MQ=255)
                                                                                                                                        aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa         <  2:27877/149‑1 (MQ=255)
                                                                                                                                        aaaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa         >  2:255122/1‑149 (MQ=255)
                                                                                                                                         aaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc        >  4:133547/1‑149 (MQ=255)
                                                                                                                                           aCATGTCCTTAGTGCAATGTTTTTATTGTTTAAATCCCCCACGTCGATTTATATTGCATTTGCATTAGCTTAGTTCTAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCTCTCTCACGc      <  4:297569/149‑1 (MQ=255)
                                                                                                                                               ggCCTTAGTGCCATGTTTTTATTGTCTAAAGCCCCCACGTCCATTAATAATGAATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTa  >  4:30891/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TGGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTA  >  NC_000913/3276740‑3277031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: