Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,462,946 T→C 5.9% V8A (GTC→GCC)  gspK → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,462,9460TC 5.9% 305.4 / 7.2 101V8A (GTC→GCC) gspKgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base T (44/51);  new base C (3/3);  total (47/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.29e-01

Only 100 of 103 total aligned reads displayed.

TTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAG  >  NC_000913/3462798‑3463088
                                                                                                                                                    |                                                                                                                                              
ttCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGt                                                                                                                                                >  8:129456/1‑149 (MQ=255)
ttCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGt                                                                                                                                                >  1:489903/1‑149 (MQ=255)
    tctctcAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCTAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg                                                                                                                                            >  5:149093/1‑149 (MQ=255)
    tctctcAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTcgcg                                                                                                                                            >  1:478537/1‑149 (MQ=255)
          aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAGCAGCGCGACGCCGCgttgt                                                                                                                                        <  7:8536/147‑2 (MQ=255)
          aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgct                                                                                                                                       <  8:1207/148‑1 (MQ=255)
           gTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                     >  8:221647/1‑149 (MQ=255)
            tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                    >  3:153524/1‑149 (MQ=255)
            tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                    >  3:23800/1‑149 (MQ=255)
            tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCGCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                    >  6:148845/1‑149 (MQ=255)
              aCGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAtt                                                                                                                                   <  8:369487/148‑1 (MQ=255)
              aCGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAtt                                                                                                                                   <  6:350315/148‑1 (MQ=255)
               cGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGt                                                                                                                                 <  5:82648/149‑1 (MQ=255)
                gTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCTCGCTGCTCATTGTa                                                                                                                                >  4:322684/1‑149 (MQ=255)
                gTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTa                                                                                                                                <  2:267471/149‑1 (MQ=255)
                   aCATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACt                                                                                                                              >  3:92277/1‑148 (MQ=255)
                     aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                           <  6:59400/149‑1 (MQ=255)
                     aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                           <  5:480159/149‑1 (MQ=255)
                     aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                           <  5:480113/149‑1 (MQ=255)
                          cAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATg                                                                                                                          <  2:478537/145‑1 (MQ=255)
                             ccTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                   >  5:294959/2‑149 (MQ=255)
                              cTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgc                                                                                                                  >  5:61122/1‑149 (MQ=255)
                               tCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcg                                                                                                                 >  1:407219/1‑149 (MQ=255)
                               tCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcg                                                                                                                 >  8:410496/1‑149 (MQ=255)
                                 aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGCCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCt                                                                                                               >  5:477534/1‑149 (MQ=255)
                                 aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                     <  7:247646/127‑1 (MQ=255)
                                 aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                     >  8:247646/1‑127 (MQ=255)
                                 aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcgc                                                                                                                <  8:474368/148‑1 (MQ=255)
                                 aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCt                                                                                                               <  5:148845/149‑1 (MQ=255)
                                   cAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCa                                                                                                             <  2:489903/149‑1 (MQ=255)
                                    aaTATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                            <  6:70595/149‑1 (MQ=255)
                                      tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGGAGAGTCGCCCGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCGTGTTACTGGCGCTCATgt                                                                                                          >  8:259233/1‑148 (MQ=255)
                                      tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGTGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                          <  5:57879/149‑1 (MQ=255)
                                      tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                          >  4:158728/1‑149 (MQ=255)
                                      tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                          <  4:23800/149‑1 (MQ=255)
                                          gggggTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGgctg                                                                                                       <  2:55312/148‑1 (MQ=255)
                                             ggTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGctct                                                                                                   <  7:449768/149‑1 (MQ=255)
                                                  cAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag                                                                                              <  1:55787/149‑1 (MQ=255)
                                                  cAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag                                                                                              <  1:63055/149‑1 (MQ=255)
                                                    gcgcCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcagca                                                                                            <  6:418946/149‑1 (MQ=255)
                                                        cGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACa                                                                                        >  8:335785/1‑149 (MQ=255)
                                                         gTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACAt                                                                                       <  6:61122/149‑1 (MQ=255)
                                                                    ccTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                   <  1:136647/110‑1 (MQ=255)
                                                                    ccTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                   >  2:136647/1‑110 (MQ=255)
                                                                     cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTgg                                                                                                 <  8:303394/127‑1 (MQ=255)
                                                                     cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTgg                                                                                                 >  7:303394/1‑127 (MQ=255)
                                                                     cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGttt                                                                           <  6:149093/149‑1 (MQ=255)
                                                                     cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGttt                                                                           >  3:300220/1‑149 (MQ=255)
                                                                      tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                            <  2:423583/115‑1 (MQ=255)
                                                                      tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                            >  1:423583/1‑115 (MQ=255)
                                                                      tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                          >  7:477207/1‑149 (MQ=255)
                                                                        aGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                          <  7:9034/147‑1 (MQ=255)
                                                                        aGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                          <  7:129456/147‑1 (MQ=255)
                                                                          cAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTcaca                                                                      <  7:263892/149‑1 (MQ=255)
                                                                               aGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCAcagccag                                                                 >  5:217644/1‑149 (MQ=255)
                                                                                gCGAGAGAAGAGTCGCCAGCTCAGACGCACGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCAcagccagc                                                                >  4:425093/1‑149 (MQ=255)
                                                                                  gagagaAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCtt                                                              >  2:439994/1‑149 (MQ=255)
                                                                                    gagaAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAACCAGCtt                                                              >  5:428992/1‑147 (MQ=255)
                                                                                     agaagaGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAg                                                           <  4:300220/149‑1 (MQ=255)
                                                                                      gaagaGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGc                                                          <  3:158728/149‑1 (MQ=255)
                                                                                            tCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAAccc                                                    <  7:221647/149‑1 (MQ=255)
                                                                                                    tCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACACCGCGGCGTCGCGCTGCTCATTGTCCTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGCCATGCCGCTCAGTTTTCACAGCCCGCTTCAGCGAACCCGCCAggg                                             >  6:37083/1‑147 (MQ=255)
                                                                                                    tCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGt                                             >  2:401529/1‑148 (MQ=255)
                                                                                                      aGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTaaa                                          >  1:270845/1‑149 (MQ=255)
                                                                                                      aGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTaaa                                          >  6:410511/1‑149 (MQ=255)
                                                                                                        aCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGc                                                  >  7:419151/1‑139 (MQ=255)
                                                                                                        aCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGc                                                  <  8:419151/139‑1 (MQ=255)
                                                                                                              gCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCatcatc                                  <  1:401529/149‑1 (MQ=255)
                                                                                                                    aataatAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAAc                            >  1:189744/1‑149 (MQ=255)
                                                                                                                     ataataACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACg                           <  2:189744/149‑1 (MQ=255)
                                                                                                                     ataataACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTAAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACg                           >  2:462388/1‑149 (MQ=255)
                                                                                                                         taaCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCaa                       <  4:24149/149‑1 (MQ=255)
                                                                                                                         taaCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCaa                       >  7:313165/1‑149 (MQ=255)
                                                                                                                           aCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAata                     <  3:425093/149‑1 (MQ=255)
                                                                                                                           aCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAat                      <  6:217644/148‑1 (MQ=255)
                                                                                                                            ccATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAatat                    <  7:335785/149‑1 (MQ=255)
                                                                                                                             cATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAatat                    >  4:179208/1‑148 (MQ=255)
                                                                                                                             cATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATg                   <  2:407219/149‑1 (MQ=255)
                                                                                                                               tGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGat                 <  5:410511/149‑1 (MQ=255)
                                                                                                                               agtacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                  >  2:26076/6‑148 (MQ=38)
                                                                                                                                 tacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAg                                               >  5:314026/4‑117 (MQ=38)
                                                                                                                                 tacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAg                                               <  6:314026/114‑1 (MQ=38)
                                                                                                                                 tacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGatat               >  7:108777/4‑149 (MQ=255)
                                                                                                                                 aataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAACCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGata                <  6:428992/148‑1 (MQ=255)
                                                                                                                                  ataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATAtt              <  4:92277/149‑1 (MQ=255)
                                                                                                                                  ataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATAtt              <  6:294959/149‑1 (MQ=255)
                                                                                                                                     atGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGaa           <  8:321257/149‑1 (MQ=255)
                                                                                                                                     atGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGaa           >  1:33080/1‑149 (MQ=255)
                                                                                                                                      tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                  >  2:265516/1‑141 (MQ=255)
                                                                                                                                      tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                  <  1:265516/141‑1 (MQ=255)
                                                                                                                                      tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAAc          >  3:144046/1‑149 (MQ=255)
                                                                                                                                      tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAAc          <  6:477534/149‑1 (MQ=255)
                                                                                                                                       gAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACCTGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACt         >  1:409534/1‑149 (MQ=255)
                                                                                                                                       gAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACt         >  6:324765/1‑149 (MQ=255)
                                                                                                                                         acaacGCGGCGTCGCGCTGCTCTTTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTgg       <  3:322684/149‑1 (MQ=255)
                                                                                                                                          cagcgcgACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGc      <  1:26076/146‑1 (MQ=255)
                                                                                                                                           aacGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGCCATGCCGCTCAGTGTTCACAGCCAGCTTCCGCGACCCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg     >  2:366495/1‑149 (MQ=255)
                                                                                                                                           aacGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg     <  4:153524/149‑1 (MQ=255)
                                                                                                                                            acGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg     >  2:41424/1‑148 (MQ=255)
                                                                                                                                              gcggcgTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGgag  <  1:439994/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                              
TTCCTCTCTCAGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAG  >  NC_000913/3462798‑3463088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: