Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,462,951 C→T 5.1% L10L (CTG→TTG)  gspK → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,462,9510CT 5.1% 317.4 / 4.1 97L10L (CTG→TTG) gspKgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base C (42/50);  new base T (3/2);  total (45/52)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01

Only 100 of 102 total aligned reads displayed.

AGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAGAAACTTGCGC  >  NC_000913/3462808‑3463098
                                                                                                                                               |                                                                                                                                                   
aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAGCAGCGCGACGCCGCgttgt                                                                                                                                                  <  7:8536/147‑2 (MQ=255)
aGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCgctgct                                                                                                                                                 <  8:1207/148‑1 (MQ=255)
 gTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                               >  8:221647/1‑149 (MQ=255)
  tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                              >  3:23800/1‑149 (MQ=255)
  tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                              >  3:153524/1‑149 (MQ=255)
  tCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCGCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAt                                                                                                                                              >  6:148845/1‑149 (MQ=255)
    aCGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAtt                                                                                                                                             <  6:350315/148‑1 (MQ=255)
    aCGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCAtt                                                                                                                                             <  8:369487/148‑1 (MQ=255)
     cGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGt                                                                                                                                           <  5:82648/149‑1 (MQ=255)
      gTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCTCGCTGCTCATTGTa                                                                                                                                          >  4:322684/1‑149 (MQ=255)
      gTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTa                                                                                                                                          <  2:267471/149‑1 (MQ=255)
         aCATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACt                                                                                                                                        >  3:92277/1‑148 (MQ=255)
           aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                                     <  5:480159/149‑1 (MQ=255)
           aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                                     <  6:59400/149‑1 (MQ=255)
           aTCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCAt                                                                                                                                     <  5:480113/149‑1 (MQ=255)
                cAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATg                                                                                                                                    <  2:478537/145‑1 (MQ=255)
                   ccTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                             >  5:294959/2‑149 (MQ=255)
                    cTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgc                                                                                                                            >  5:61122/1‑149 (MQ=255)
                     tCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcg                                                                                                                           >  1:407219/1‑149 (MQ=255)
                     tCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcg                                                                                                                           >  8:410496/1‑149 (MQ=255)
                       aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGCCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCt                                                                                                                         >  5:477534/1‑149 (MQ=255)
                       aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                               >  8:247646/1‑127 (MQ=255)
                       aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCa                                                                                                                                               <  7:247646/127‑1 (MQ=255)
                       aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGgcgc                                                                                                                          <  8:474368/148‑1 (MQ=255)
                       aGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCt                                                                                                                         <  5:148845/149‑1 (MQ=255)
                         cAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCa                                                                                                                       <  2:489903/149‑1 (MQ=255)
                          aaTATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                                      <  6:70595/149‑1 (MQ=255)
                            tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGGAGAGTCGCCCGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCGTGTTACTGGCGCTCATgt                                                                                                                    >  8:259233/1‑148 (MQ=255)
                            tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGTGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                                    <  5:57879/149‑1 (MQ=255)
                            tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                                    >  4:158728/1‑149 (MQ=255)
                            tatGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATgg                                                                                                                    <  4:23800/149‑1 (MQ=255)
                                gggggTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGgctg                                                                                                                 <  2:55312/148‑1 (MQ=255)
                                   ggTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGctct                                                                                                             <  7:449768/149‑1 (MQ=255)
                                        cAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag                                                                                                        <  1:55787/149‑1 (MQ=255)
                                        cAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcag                                                                                                        <  1:63055/149‑1 (MQ=255)
                                          gcgcCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGgcagca                                                                                                      <  6:418946/149‑1 (MQ=255)
                                              cGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACa                                                                                                  >  8:335785/1‑149 (MQ=255)
                                               gTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACAt                                                                                                 <  6:61122/149‑1 (MQ=255)
                                                          ccTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                             <  1:136647/110‑1 (MQ=255)
                                                          ccTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTgg                                                                                                                             >  2:136647/1‑110 (MQ=255)
                                                          ccTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtt                                                                                      >  3:24149/1‑149 (MQ=255)
                                                           cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTgg                                                                                                           >  7:303394/1‑127 (MQ=255)
                                                           cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTgg                                                                                                           <  8:303394/127‑1 (MQ=255)
                                                           cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGttt                                                                                     <  6:149093/149‑1 (MQ=255)
                                                           cTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGttt                                                                                     >  3:300220/1‑149 (MQ=255)
                                                            tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                                      <  2:423583/115‑1 (MQ=255)
                                                            tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCAt                                                                                                                      >  1:423583/1‑115 (MQ=255)
                                                            tGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                                    >  7:477207/1‑149 (MQ=255)
                                                              aGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                                    <  7:9034/147‑1 (MQ=255)
                                                              aGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGtttt                                                                                    <  7:129456/147‑1 (MQ=255)
                                                                cAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTcaca                                                                                <  7:263892/149‑1 (MQ=255)
                                                                     aGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCAcagccag                                                                           >  5:217644/1‑149 (MQ=255)
                                                                      gCGAGAGAAGAGTCGCCAGCTCAGACGCACGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCAcagccagc                                                                          >  4:425093/1‑149 (MQ=255)
                                                                        gagagaAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCtt                                                                        >  2:439994/1‑149 (MQ=255)
                                                                         agagaAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCtt                                                                        >  6:360780/1‑148 (MQ=255)
                                                                          gagaAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAACCAGCtt                                                                        >  5:428992/1‑147 (MQ=255)
                                                                           agaagaGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAg                                                                     <  4:300220/149‑1 (MQ=255)
                                                                            gaagaGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGc                                                                    <  3:158728/149‑1 (MQ=255)
                                                                                  tCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAAccc                                                              <  7:221647/149‑1 (MQ=255)
                                                                                          tCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACACCGCGGCGTCGCGCTGCTCATTGTCCTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGCCATGCCGCTCAGTTTTCACAGCCCGCTTCAGCGAACCCGCCAggg                                                       >  6:37083/1‑147 (MQ=255)
                                                                                          tCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGt                                                       >  2:401529/1‑148 (MQ=255)
                                                                                            aGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTaaa                                                    >  1:270845/1‑149 (MQ=255)
                                                                                            aGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTaaa                                                    >  6:410511/1‑149 (MQ=255)
                                                                                              aCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGc                                                            >  7:419151/1‑139 (MQ=255)
                                                                                              aCGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGc                                                            <  8:419151/139‑1 (MQ=255)
                                                                                                    gCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCatcatc                                            <  1:401529/149‑1 (MQ=255)
                                                                                                          aataatAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAAc                                      >  1:189744/1‑149 (MQ=255)
                                                                                                           ataataACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACg                                     <  2:189744/149‑1 (MQ=255)
                                                                                                           ataataACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTAAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACg                                     >  2:462388/1‑149 (MQ=255)
                                                                                                               taaCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCaa                                 >  7:313165/1‑149 (MQ=255)
                                                                                                               taaCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCaa                                 <  4:24149/149‑1 (MQ=255)
                                                                                                                 aCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAata                               <  3:425093/149‑1 (MQ=255)
                                                                                                                 aCCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAat                                <  6:217644/148‑1 (MQ=255)
                                                                                                                  ccATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAatat                              <  7:335785/149‑1 (MQ=255)
                                                                                                                   cATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAatat                              >  4:179208/1‑148 (MQ=255)
                                                                                                                   cATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATg                             <  2:407219/149‑1 (MQ=255)
                                                                                                                     tGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGat                           <  5:410511/149‑1 (MQ=255)
                                                                                                                     agtacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                            >  2:26076/6‑148 (MQ=38)
                                                                                                                       tacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAg                                                         <  6:314026/114‑1 (MQ=38)
                                                                                                                       tacaatGAGCAGCGCGACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGatat                         >  7:108777/4‑149 (MQ=255)
                                                                                                                       aataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAACCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGata                          <  6:428992/148‑1 (MQ=255)
                                                                                                                        ataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATAtt                        <  4:92277/149‑1 (MQ=255)
                                                                                                                        ataatGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATAtt                        <  6:294959/149‑1 (MQ=255)
                                                                                                                           atGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGaa                     >  1:33080/1‑149 (MQ=255)
                                                                                                                           atGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGaa                     <  8:321257/149‑1 (MQ=255)
                                                                                                                            tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                            <  1:265516/141‑1 (MQ=255)
                                                                                                                            tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGa                            >  2:265516/1‑141 (MQ=255)
                                                                                                                            tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAAc                    >  3:144046/1‑149 (MQ=255)
                                                                                                                            tGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAAc                    <  6:477534/149‑1 (MQ=255)
                                                                                                                             gAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACCTGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACt                   >  1:409534/1‑149 (MQ=255)
                                                                                                                             gAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACt                   >  6:324765/1‑149 (MQ=255)
                                                                                                                               acaacGCGGCGTCGCGCTGCTCTTTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTgg                 <  3:322684/149‑1 (MQ=255)
                                                                                                                                cagcgcgACGCCGCGTTGTTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGc                <  1:26076/146‑1 (MQ=255)
                                                                                                                                 aacGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGCCATGCCGCTCAGTGTTCACAGCCAGCTTCCGCGACCCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg               >  2:366495/1‑149 (MQ=255)
                                                                                                                                 aacGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg               <  4:153524/149‑1 (MQ=255)
                                                                                                                                  acGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCg               >  2:41424/1‑148 (MQ=255)
                                                                                                                                    gcggcgTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGgag            <  1:439994/149‑1 (MQ=255)
                                                                                                                                            gcgcTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAGAAACTTg     <  5:360780/148‑1 (MQ=255)
                                                                                                                                             cgcTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACTCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAGAAACTTgcg   <  5:479417/149‑1 (MQ=255)
                                                                                                                                              gctgctCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAGAAACTTgcgc  >  1:341222/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
AGTCACGTTACATCTGCAGACTCAGCAATATGGGGGGTTACAGCGCCGTTTTGCATTACCTGAGCAGTTAGCGAGAGAAGAGTCGCCAGCTCAGACGCAGGCCGGGAATAATAACCATGAATAATGAACAACGCGGCGTCGCGCTGCTCATTGTACTCATGTTACTGGCGCTCATGGCTGCTCTGGCAGCAGACATGACGCTCAGTTTTCACAGCCAGCTTCAGCGAACCCGCCAGGTAAACCATCATCTGCAACGGCAATATGATATTGAACTGGCGGAGAAACTTGCGC  >  NC_000913/3462808‑3463098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: