Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,711,252 T→A 8.5% A252A (GCA→GCT)  yhjX ← pyruvate‑inducible inner membrane protein, putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,711,2520TA 8.5% 136.9 / 4.3 47A252A (GCA→GCT) yhjXpyruvate‑inducible inner membrane protein, putative transporter
Reads supporting (aligned to +/- strand):  ref base T (20/22);  new base A (2/2);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

AACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCGTGTAATCT  >  NC_000913/3711112‑3711399
                                                                                                                                            |                                                                                                                                                   
aaCAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAAc                                                                                                                                             <  6:377306/149‑1 (MQ=255)
   agcagGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACcac                                                                                                                                          <  8:1564/149‑1 (MQ=255)
      agGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACAt                                                                                                                                        >  8:87847/1‑148 (MQ=255)
         gccgccATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAg                                                                                                                                    <  8:404067/149‑1 (MQ=255)
         gccgccATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAg                                                                                                                                    <  8:126490/149‑1 (MQ=255)
              cATACCCACCAGCGATATCCCCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCAGCGGAAACCACATCAAGGtgtg                                                                                                                               >  4:315957/1‑149 (MQ=255)
              cATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCAGCGGAAACCACATCAAGGtgtg                                                                                                                               >  4:309889/1‑149 (MQ=255)
               aTACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGc                                                                                                                              <  2:410541/149‑1 (MQ=255)
                  cccACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCa                                                                                                                            <  2:379789/148‑1 (MQ=255)
                   ccaccaGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGa                                                                                                                          >  8:203366/1‑149 (MQ=255)
                       caGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACttt                                                                                                                      <  1:417159/149‑1 (MQ=255)
                         gCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGTTTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATATCAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTgg                                                                                                                    <  8:240162/149‑1 (MQ=255)
                         gCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTgg                                                                                                                    <  7:203366/149‑1 (MQ=255)
                          cGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGGGTGCCAGACTTTggg                                                                                                                   <  4:324496/149‑1 (MQ=255)
                           gATTTCACCTGACCAATTGTAATAACACGTATACGGGCGATTTTGTCAGACAGAATACCCAGCACAAGACCACCTGAAAGGTTGGCGATGGAAATTACTGTGACTCCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTggg                                                                                                                   <  6:427038/148‑1 (MQ=255)
                             tatCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGa                                                                                                                <  8:240175/149‑1 (MQ=255)
                                        cAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGc                                                                                                      >  4:15522/1‑148 (MQ=255)
                                                 taaCACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAAt                                                                                             >  7:115208/1‑148 (MQ=255)
                                                 taaCACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGAAGACCTGAAAGGTTGGCGATGGAAATAACAGTTACTGCATAGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGTGCTATATCTTTCGCTACCCAAAt                                                                                             >  7:108227/1‑148 (MQ=255)
                                                         aTACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAg                                                                                    <  7:87847/149‑1 (MQ=255)
                                                             gggCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCg                                                                                >  5:269930/1‑149 (MQ=255)
                                                              ggCGATTTTGTGAGACAGAATACCCAGCAACAGACTACCTGAAAGGTTGGCGATGGAAAAAAGAGTGACTGCATTGGCTGCTGAAACCACATCTAGGTGTGCCATACTTTGGGCGATATCTTTc                                                                                                        <  5:385502/124‑1 (MQ=255)
                                                              ggCGATTTTGTCAGACAGAATACACAGCACCAGACGCCCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAACCCACATCAAGGTGTGACAGACTATTGGCGATATCTTTc                                                                                                        >  6:385502/1‑124 (MQ=255)
                                                                     ttGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGc                                                                         <  3:15522/148‑1 (MQ=255)
                                                                       gTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAg                                                                       <  6:269930/148‑1 (MQ=255)
                                                                          agacagaATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCgg                                                                   >  4:136538/1‑149 (MQ=255)
                                                                             cagaATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCa                                                                <  3:136538/149‑1 (MQ=255)
                                                                                     ccAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACAt                                                         >  6:110048/1‑148 (MQ=255)
                                                                                       aGCACCAGCCGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCAGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTAc                                                      <  3:315957/149‑1 (MQ=255)
                                                                                       aGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCAGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTAc                                                      <  3:309889/149‑1 (MQ=255)
                                                                                            cAGCCGACCTGAAAGGTTGGCGATGGAAAAAACAGTGACTGCATTCGCTGAGGAAACCACATCAAGGTGTGCCAGACTTTGGTCGATAACTTTCGCTACCCAAAACCCGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCt                                                  >  8:12884/1‑148 (MQ=255)
                                                                                            cAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCt                                                  >  4:231890/1‑148 (MQ=255)
                                                                                                      gAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGTCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTa                                       >  6:64045/1‑149 (MQ=255)
                                                                                                       aaaGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTc                                                                                                        >  1:208319/1‑83 (MQ=255)
                                                                                                       aaaGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTc                                                                                                        <  2:208319/83‑1 (MQ=255)
                                                                                                       aaaGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTAc                                      >  7:310967/1‑149 (MQ=255)
                                                                                                       aaaGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTAc                                      >  4:392868/1‑149 (MQ=255)
                                                                                                            ttGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGGGATATCTTTCGCTCCCCCAATCACGTTCAGGACGCTCATGCGGGCGGTCAGGAACATTACGGCTAACATCCAGTACTGCgg                                 >  6:9284/1‑149 (MQ=255)
                                                                                                                      aaaTAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATc                       <  3:392868/149‑1 (MQ=255)
                                                                                                                        aTAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGa                     >  1:118635/1‑149 (MQ=255)
                                                                                                                                     cATTGGCTGCGGAAACGACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCgt         <  8:115208/148‑1 (MQ=255)
                                                                                                                                     cATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCgtg        >  6:439250/1‑149 (MQ=255)
                                                                                                                                     cATTGGCGGCGGAAACCACATCAAGGTGTGACAGACTTTGGGCGATATCTTTCGCTACCCAAATCACGTACATGCCGGTCATGGAGGCGGTCAGGAACATTACCGCTACCATCCAGTACTGCGGTTTACGCTTCGACTCTGCCAGCgt         <  8:108227/148‑1 (MQ=255)
                                                                                                                                      aTTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGAACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCgtg        >  4:70231/1‑148 (MQ=255)
                                                                                                                                         ggCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGATGATGCAGGCGGTCAGGATCATTACCGCTAACATCCAGTTCTGCGGTTTACGCATCGACTCTGCCAGCGTGTaa     <  7:12884/148‑1 (MQ=255)
                                                                                                                                          gCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCGTGTAATc   >  6:340944/1‑149 (MQ=255)
                                                                                                                                           cTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCGTGTAATCt  <  8:310967/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                   
AACAGCAGGGCCGCCATACCCACCAGCGATATCACCTGACCAATGGTAATAACACGGATACGGGCGATTTTGTCAGACAGAATACCCAGCACCAGACGACCTGAAAGGTTGGCGATGGAAATAACAGTGACTGCATTGGCTGCGGAAACCACATCAAGGTGTGCCAGACTTTGGGCGATATCTTTCGCTACCCCAATCACGTACAGGCCGCTCATGCAGGCGGTCAGGAACATTACCGCTAACATCCAGTACTGCGGTTTACGCATCGACTCTGCCAGCGTGTAATCT  >  NC_000913/3711112‑3711399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑