Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,197,139 T→C 5.0% F79F (TTT→TTC)  nudC → NADH pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,197,1390TC 5.0% 247.9 / 2.0 79F79F (TTT→TTC) nudCNADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (32/43);  new base C (2/2);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

TGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCC  >  NC_000913/4196992‑4197287
                                                                                                                                                   |                                                                                                                                                    
tGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCTAATGGCAGTGGGAACCTGTTTGTTTAGTACAACAGCATCGGCGTCACGATATGGGGGCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTc                                                                                                                                                     <  1:183327/149‑1 (MQ=255)
tGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGGTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTc                                                                                                                                                     <  1:49000/149‑1 (MQ=255)
   cGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAAc                                                                                                                                                  <  6:480196/149‑1 (MQ=255)
    gAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACt                                                                                                                                                 <  3:236595/149‑1 (MQ=255)
    gAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACt                                                                                                                                                 >  4:58072/1‑149 (MQ=255)
     aaGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTg                                                                                                                                                >  4:149055/1‑149 (MQ=255)
     aaGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTg                                                                                                                                                >  7:228277/1‑149 (MQ=255)
      aGCGGCAAATTTCGATCTTGTGGGGCAGCGCGCACTACAAATCGGCGAAGGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTgg                                                                                                                                               <  7:325495/149‑1 (MQ=255)
           cAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGa                                                                                                                                          <  3:317128/149‑1 (MQ=255)
               tttCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACACCAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTCCAACTGGCCGGACGAg                                                                                                                                      >  5:336311/1‑149 (MQ=255)
               tttCGATCTTGTGGGTCAGCGCGCACTACAAAACGGCGAATGGCAGGGGGCACCTGGTTGGTTAGTACAACAGCAGCGGCGGCACGATATGGGATCGGTCCGTCAGGTCAGTGATCGCTCTGTGTGGCTGTTTCAACTGGCCGCActcg                                                                                                                                      >  4:351347/1‑146 (MQ=255)
                             gTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCg                                                                                                                        >  4:172716/1‑149 (MQ=255)
                              tCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCg                                                                                                                        >  1:240963/1‑148 (MQ=255)
                               cAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCGCGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGa                                                                                                                      <  2:497503/149‑1 (MQ=255)
                                 gcgcgcACTACAAATCGGCGAATGGCAGGGGGAACGTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTCCAACTGGCCGGACGAGGCGTACAACTGGCGGAGt                                                                                                                    <  6:336311/149‑1 (MQ=255)
                                    cgcCCTACAAATCGGCGACTGGCAGGGGGAACCTGGTTGGTTAGTACAACAGCAGCGGCGACACGATATTGGGTCTGTACGTCAGGTCTTTGATCTCTATGTTGGTCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGtttt                                                                                                                 <  2:126448/149‑1 (MQ=255)
                                       aCTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTAcc                                                                                                              >  6:26249/1‑149 (MQ=255)
                                         tACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGa                                                                                                            >  2:446190/1‑149 (MQ=255)
                                         tACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGa                                                                                                            >  6:432335/1‑149 (MQ=255)
                                          aCAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTa                                                                                                                                 <  8:185038/127‑1 (MQ=255)
                                           cAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGAt                                                                                                           <  3:58072/148‑1 (MQ=255)
                                           cAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATc                                                                                                          <  6:279680/149‑1 (MQ=255)
                                           cAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGGCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATc                                                                                                          <  6:459101/149‑1 (MQ=255)
                                           cAAATCGGCGAATCTCAGGGGGAACCTTTTTGGTTTTTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGa                                                                                                            <  6:417677/147‑1 (MQ=255)
                                            ataTCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTTCAAAAGCAGCGGCGTGCCGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCg                                                                                                         <  7:425170/147‑1 (MQ=255)
                                             aaTCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGc                                                                                                        <  5:114832/149‑1 (MQ=255)
                                              aTCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCa                                                                                                       >  4:152575/1‑149 (MQ=255)
                                                    gAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATa                                                                                                 <  8:228277/149‑1 (MQ=255)
                                                    gAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATa                                                                                                 <  3:149055/149‑1 (MQ=255)
                                                       tGGCAGTTGTAACCTGTTTGGTTAGTACAAAAGCAGCGGCGTCACTATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGAATAAATACtg                                                                                              <  5:343734/149‑1 (MQ=255)
                                                         tcAGGGGGTACCTGTTTGGTTAGTACAACCGCAGCTGCGGAACTATATGGGGTCGGTAAGTCCGGTCATTGCTATCGATGGTGGGATGTTTCAACTGGCCGGACTAGGCGGACAACTGGCGGAGTTTTACCGATCGCATAAATACtgtg                                                                                            <  3:351347/148‑1 (MQ=255)
                                                            gggggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGt                                                                                          >  3:100132/1‑148 (MQ=255)
                                                               ggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTG‑TCCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtg                                                                                              >  8:214767/1‑140 (MQ=255)
                                                               ggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTG‑TCCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtg                                                                                              <  7:214767/140‑1 (MQ=255)
                                                                       gttGGTTAGGACAACATCAGCGGCGTCAAGATATTGGGTCGGTACGTCAGGTCATTGTTCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGc                                                                                <  4:100132/146‑1 (MQ=255)
                                                                           gTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGaaa                                                                          <  5:432335/149‑1 (MQ=255)
                                                                              aGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCgg                                                                                  >  2:81194/1‑138 (MQ=255)
                                                                              aGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCgg                                                                                  <  1:81194/138‑1 (MQ=255)
                                                                                     cagcagCTGCGTCACGTTATGGGGTCGGTACGTCATGGCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAg                                                                <  4:289810/149‑1 (MQ=255)
                                                                                     cagcagCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCGGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGATTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGa                                                                 <  7:365142/148‑1 (MQ=255)
                                                                                     cagcagCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCGGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAAGGTATcc                                                                   >  8:365142/1‑146 (MQ=255)
                                                                                      agcagcGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAg                                                                >  1:201305/1‑148 (MQ=255)
                                                                                        cagcGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTAt                                                                     <  3:271546/141‑1 (MQ=255)
                                                                                        cagcGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTAt                                                                     >  4:271546/1‑141 (MQ=255)
                                                                                              cgTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGa                                                       <  7:163007/149‑1 (MQ=255)
                                                                                                tCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCAt                                                                              >  6:445729/1‑124 (MQ=255)
                                                                                                tCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCAt                                                                              <  5:445729/124‑1 (MQ=255)
                                                                                                  aCGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATgg                                                   >  5:94635/1‑149 (MQ=255)
                                                                                                  aCGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCAGAGCAAAACCGAATgg                                                   >  6:284648/1‑149 (MQ=255)
                                                                                                    gATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATTGGc                                                 >  5:427183/1‑149 (MQ=255)
                                                                                                       atGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGTCGTACAACTGTCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAATACCGAATGGGCGa                                               <  2:59141/148‑1 (MQ=255)
                                                                                                        tGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATg                                             <  1:446190/149‑1 (MQ=255)
                                                                                                         ggggTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGTCATGAAATGTATCCGAGCAAAACCGAATGGGCGATg                                             <  5:26249/148‑1 (MQ=255)
                                                                                                            gTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCtgt                                         >  5:181207/1‑149 (MQ=255)
                                                                                                              cGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGc                                       <  3:172716/149‑1 (MQ=255)
                                                                                                               ggTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtgtg                                                                                            <  3:157753/95‑1 (MQ=255)
                                                                                                               ggTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtgtg                                                                                            >  4:157753/1‑95 (MQ=255)
                                                                                                               ggTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCtgtg                                        >  1:311533/1‑147 (MQ=255)
                                                                                                                gTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAg                                     >  5:6046/1‑149 (MQ=255)
                                                                                                                   cGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGcc                                   <  2:240963/148‑1 (MQ=255)
                                                                                                                      cAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCgg                                                                                  <  3:118985/98‑1 (MQ=255)
                                                                                                                      cAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCgg                                                                                  >  4:118985/1‑98 (MQ=255)
                                                                                                                       aGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTg                               <  6:94635/148‑1 (MQ=255)
                                                                                                                       aGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTg                               <  2:311533/148‑1 (MQ=255)
                                                                                                                       aGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTg                               <  3:152575/148‑1 (MQ=255)
                                                                                                                           cATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAAcc                                                         >  7:147148/1‑118 (MQ=255)
                                                                                                                           cATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAAcc                                                         <  8:147148/118‑1 (MQ=255)
                                                                                                                            aTTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtgtg                                                                                            >  7:104773/1‑82 (MQ=255)
                                                                                                                            aTTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACtgtg                                                                                            <  8:104773/82‑1 (MQ=255)
                                                                                                                             ttGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAg                        >  8:226531/1‑149 (MQ=255)
                                                                                                                                aTCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGt                     >  2:10849/1‑149 (MQ=255)
                                                                                                                                aTCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGt                     >  1:130429/1‑149 (MQ=255)
                                                                                                                                 tctcGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATggg                                                  >  4:162585/1‑119 (MQ=255)
                                                                                                                                   tcGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTtac                  >  5:138696/1‑149 (MQ=255)
                                                                                                                                     gATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGtt                    >  2:183357/1‑145 (MQ=255)
                                                                                                                                     gATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGtt                    <  1:183357/145‑1 (MQ=255)
                                                                                                                                     gATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTta                   <  6:181207/146‑1 (MQ=255)
                                                                                                                                              cTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAAt       <  6:6046/149‑1 (MQ=255)
                                                                                                                                               tGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATc      <  6:427183/149‑1 (MQ=255)
                                                                                                                                                   tCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGccc  >  5:95999/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGCGAAGCGGCAAATTTCGATCTTGTGGGTCAGCGCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCCC  >  NC_000913/4196992‑4197287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑