Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,388,502 G→C 5.4% A290G (GCC→GGC)  mscM ← mechanosensitive channel protein, miniconductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,388,5020GC 5.4% 318.2 / 7.4 93A290G (GCC→GGC) mscMmechanosensitive channel protein, miniconductance
Reads supporting (aligned to +/- strand):  ref base G (45/43);  new base C (3/2);  total (48/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 101 total aligned reads displayed.

GGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCA  >  NC_000913/4388357‑4388649
                                                                                                                                                 |                                                                                                                                                   
ggTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCa                                                                                                                                                   <  5:364378/148‑1 (MQ=255)
 gTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGc                                                                                                                                                 >  1:334045/1‑149 (MQ=255)
 gTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGACACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGc                                                                                                                                                 <  7:57259/149‑1 (MQ=255)
 ctATCAAGCTGTTGTGGTTTCGGCATTTCCGTCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTTGCAGc                                                                                                                                                 <  6:13827/148‑1 (MQ=255)
   aTCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCt                                                                                                                                               >  4:192251/1‑149 (MQ=255)
    tCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCt                                                                                                                                               <  2:340499/148‑1 (MQ=255)
      acgCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGAc                                                                                                                                            <  1:224553/147‑1 (MQ=255)
      aaGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGATCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGa                                                                                                                                             <  7:350599/148‑1 (MQ=255)
       aGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACg                                                                                                                                           >  5:81662/1‑149 (MQ=255)
            ttgtGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGtt                                                                                                                                      <  1:204008/149‑1 (MQ=255)
              gtgGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTg                                                                                                                                     <  5:32194/148‑1 (MQ=255)
              gtgGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGc                                                                                                                                    >  5:414263/1‑149 (MQ=255)
                ggTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGa                                                                                                                                  <  1:445361/149‑1 (MQ=255)
                 gTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAg                                                                                                                                 <  2:249679/149‑1 (MQ=255)
                 gTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAg                                                                                                                                 >  7:433718/1‑149 (MQ=255)
                  tttCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAgg                                                                                                                                >  4:454922/1‑149 (MQ=255)
                      ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCaa                                                                                                                             <  1:69294/148‑1 (MQ=255)
                      ggCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc                                                                                                                            >  8:169486/1‑149 (MQ=255)
                       gCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGACgt                                                                                                                                          <  1:96240/134‑2 (MQ=255)
                       gCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCAGGCTGCCAGCCAGACgt                                                                                                                                          >  2:96240/1‑133 (MQ=255)
                        cATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc                                                                                                                            >  7:58048/1‑147 (MQ=255)
                         aTTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGGGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAAc                                                                                                                            >  1:155156/1‑146 (MQ=255)
                                    ccGGGCCCCCTGCGCCCGCAGCGCGTCGCCGAGCAGATTGGACGCGCCCCGCCATTGCGACTGTTCACGCAGCGTCTGCCACGCCTGCCGGACCTGTAACGTCTTGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCt                                                                                                              <  7:21238/149‑1 (MQ=255)
                                      gTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTgc                                                                                                            >  3:206456/1‑149 (MQ=255)
                                         ccACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCg                                                                                                                <  4:86695/142‑1 (MQ=255)
                                         ccACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCg                                                                                                                >  3:86695/1‑142 (MQ=255)
                                         ccACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGcc                                                                                                         >  1:116225/1‑149 (MQ=255)
                                           aCCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc                                                                                                                      >  8:232760/1‑134 (MQ=255)
                                           aCCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGatc                                                                                                                      <  7:232760/134‑1 (MQ=255)
                                           aCCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTg                                                                                                       >  6:388050/1‑149 (MQ=255)
                                           aCCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTg                                                                                                       >  5:109157/1‑149 (MQ=255)
                                            ccTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGt                                                                                                      <  6:81662/149‑1 (MQ=255)
                                            ccTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGt                                                                                                      <  5:367205/149‑1 (MQ=255)
                                             cTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGtt                                                                                                     <  5:388050/149‑1 (MQ=255)
                                                cgcCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGa                                                                                                   <  8:433718/148‑1 (MQ=255)
                                                cgcCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAt                                                                                                  <  8:318716/149‑1 (MQ=255)
                                                 gcCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAtt                                                                                                 <  2:152758/149‑1 (MQ=255)
                                                   ccGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTc                                                                                                >  2:379415/1‑148 (MQ=255)
                                                     gCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa                                                                                             <  4:206212/149‑1 (MQ=255)
                                                          gcTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc                                                                                        <  2:116225/149‑1 (MQ=255)
                                                                        aTTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGt                                                                          <  1:39600/149‑1 (MQ=255)
                                                                            gACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGtt                                                                                                                                      >  8:332815/1‑85 (MQ=255)
                                                                            gACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGtt                                                                                                                                      <  7:332815/85‑1 (MQ=255)
                                                                             aCGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAtt                                                                     <  6:414263/149‑1 (MQ=255)
                                                                                   ccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGaa                                                                <  2:334045/148‑1 (MQ=255)
                                                                                   ccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGaa                                                                <  2:336146/148‑1 (MQ=255)
                                                                                   ccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAAt                                                               >  1:207676/1‑149 (MQ=255)
                                                                                   ccAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAAt                                                               >  1:5685/1‑149 (MQ=255)
                                                                                    cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa                                                                                             <  4:152096/118‑1 (MQ=255)
                                                                                    cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCaaa                                                                                             >  3:152096/1‑118 (MQ=255)
                                                                                    cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAAtt                                                              >  7:505999/1‑149 (MQ=255)
                                                                                    cAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAAtt                                                              >  3:69864/1‑149 (MQ=255)
                                                                                     aGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTg                                                             >  7:18789/1‑149 (MQ=255)
                                                                                      gCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgc                                                            <  2:207676/149‑1 (MQ=255)
                                                                                       ccATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTgcg                                                           >  7:356142/1‑149 (MQ=255)
                                                                                          ttGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcga                                                        >  2:183177/1‑149 (MQ=255)
                                                                                            gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt                                                                                 <  6:189894/122‑1 (MQ=255)
                                                                                            gCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGt                                                                                 >  5:189894/1‑122 (MQ=255)
                                                                                             cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGAc                                                                                                                                            <  1:166054/62‑1 (MQ=255)
                                                                                             cGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGAc                                                                                                                                            >  2:166054/1‑62 (MQ=255)
                                                                                              gACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATa                                                                                   >  1:497724/1‑118 (MQ=255)
                                                                                              gACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATa                                                                                   <  2:497724/118‑1 (MQ=255)
                                                                                              gACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcgacga                                                     >  2:275655/1‑148 (MQ=255)
                                                                                                     cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt                                              <  4:69864/148‑1 (MQ=255)
                                                                                                     cACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCttt                                              <  7:169486/148‑1 (MQ=255)
                                                                                                      aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc                                                                                        >  7:348814/1‑105 (MQ=255)
                                                                                                      aCGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAgccgc                                                                                        <  8:348814/105‑1 (MQ=255)
                                                                                                       cGCAGCGTATTCAACGCCTGACGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCgg                                           <  6:109157/149‑1 (MQ=255)
                                                                                                           gCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCaaa                                       >  4:404015/1‑149 (MQ=255)
                                                                                                               aTTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCgg                                   >  5:167042/1‑149 (MQ=255)
                                                                                                                ttCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGGCGATAGTTCGCGGTTAATTATGAATTGCGCGACGATATCTTTCGGCGAATCGgc                                  <  2:155156/149‑1 (MQ=255)
                                                                                                                   aaCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGgcgc                                <  4:206456/148‑1 (MQ=255)
                                                                                                                   aaCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCt                               >  7:457431/1‑149 (MQ=255)
                                                                                                                   aaCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCt                               >  3:441936/1‑149 (MQ=255)
                                                                                                                   aaCGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCt                               >  4:421597/1‑149 (MQ=255)
                                                                                                                     cGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGt                             <  2:287463/149‑1 (MQ=255)
                                                                                                                       ccggCCGGACCTGTAACGTCTGGCTGGCCGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATGCAAAGCCGCCGATAGTTAGCGCTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGttt                           >  8:352889/4‑149 (MQ=255)
                                                                                                                           ccGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCt                        <  1:379415/148‑1 (MQ=255)
                                                                                                                           ccGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCt                        <  4:441936/148‑1 (MQ=255)
                                                                                                                             ggACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCt                                                >  7:455888/1‑122 (MQ=255)
                                                                                                                                  aacagcGTCAGGCTGCCAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGAGAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCaa                >  1:440264/6‑149 (MQ=255)
                                                                                                                                   gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAt               >  4:159436/1‑149 (MQ=255)
                                                                                                                                   gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAt               >  3:35240/1‑149 (MQ=255)
                                                                                                                                   gTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAt               >  8:189081/1‑149 (MQ=255)
                                                                                                                                   acagcGTCAGGCTGCCAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcga                                                        >  3:10500/5‑108 (MQ=255)
                                                                                                                                   acagcGTCAGGCTGCCAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGcga                                                        <  4:10500/104‑1 (MQ=255)
                                                                                                                                    tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCACCGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAAGTTTGCATTGCGCGACGATATCGTTCGGCAAATCGGCGGTGGTTTCTGCCAGCACtt              >  6:283711/1‑149 (MQ=255)
                                                                                                                                    tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCGGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGGAAGCAAta              >  4:259185/1‑148 (MQ=255)
                                                                                                                                    tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAtt              >  6:82959/1‑149 (MQ=255)
                                                                                                                                    tAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGAGTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCTCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAAtt              >  6:276794/1‑149 (MQ=255)
                                                                                                                                      aCGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCg            <  3:454922/149‑1 (MQ=255)
                                                                                                                                         tCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTa         >  5:338803/1‑149 (MQ=255)
                                                                                                                                           aggctgacAGCCAGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACt       >  8:306378/2‑149 (MQ=255)
                                                                                                                                            ggctggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACtt      >  3:120222/1‑149 (MQ=255)
                                                                                                                                               tggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTc    >  2:418682/1‑148 (MQ=255)
                                                                                                                                                ggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCg            >  6:28001/1‑139 (MQ=255)
                                                                                                                                                ggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCg            <  5:28001/139‑1 (MQ=255)
                                                                                                                                                ggcAGCCTGACGCTGTTGCGAGGCAACGAGATCCAACCGATGCGCCTGTTGATTCAAAGCCGCCGATAGATCGCGCTTAATTTTGAATTGCGCGACGATATCTATCGGCAAATCGGCGCTCTATTCTGCCAGCAATTCGGTACTTTc    >  6:19839/1‑147 (MQ=255)
                                                                                                                                                 gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCa  <  7:467350/148‑1 (MQ=255)
                                                                                                                                                 gcAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCa  <  5:127952/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GGTATCAAGCTGTTGTGGTTTCGGCATTTCCGGCAGCCGTGCCACCTGCGCCCGCAGCGCTTCGCCGAGCAGATTGGACGATCCCAGCCATTGCGACTGTTCACGCAGCGTATTCAACGCCTGCCGGACCTGTAACGTCTGGCTGGCAGCCTGACGCTGTTGCGAGGCAACGAGATCCATCCGCTGCGCCTGTTGATTCAAAGCCGCCGATAGTTCGCGGTTAATTTTGAATTGCGCGACGATATCTTTCGGCAAATCGGCGCTGTTTTCTGCCAGCAATTCGGTACTTTCCA  >  NC_000913/4388357‑4388649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: