Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,488,427 A→C 5.1% intergenic (+27/+134) lptG → / ← yjgR lipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,488,4270AC 5.1% 426.2 / 11.0 133intergenic (+27/+134)lptG/yjgRlipopolysaccharide export ABC permease/DUF853 family protein with NTPase fold
Reads supporting (aligned to +/- strand):  ref base A (54/72);  new base C (5/2);  total (59/74)
Fisher's exact test for biased strand distribution p-value = 2.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01

Only 100 of 134 total aligned reads displayed.

ACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCC  >  NC_000913/4488280‑4488575
                                                                                                                                                   |                                                                                                                                                    
aCTGGACCAGATCTGCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGACAAGCGCCAGCTTCTTCTTAATCAGCATGTGGCTGTTATGGATAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAg                                                                                                                                                     >  2:109466/1‑149 (MQ=255)
   ggACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTc                                                                                                                                                  >  2:442248/1‑149 (MQ=255)
    gACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTcc                                                                                                                                                 >  3:311767/1‑149 (MQ=255)
     aCCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTccc                                                                                                                                                <  3:360714/149‑1 (MQ=255)
     aCCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTccc                                                                                                                                                >  7:426620/1‑149 (MQ=255)
      ccAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCt                                                                                                                                               >  4:238213/1‑149 (MQ=255)
        aGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCtt                                                                                                                                              >  4:610/1‑148 (MQ=255)
        aGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCtt                                                                                                                                              >  5:48925/1‑148 (MQ=255)
         gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCTTTCGCTCCCTtta                                                                                                                                            <  3:610/149‑1 (MQ=255)
         gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCttt                                                                                                                                             <  1:442769/148‑1 (MQ=255)
         gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta                                                                                                                                            <  3:439306/149‑1 (MQ=255)
         gATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta                                                                                                                                            <  2:403779/149‑1 (MQ=255)
          aTCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttat                                                                                                                                           >  4:108933/1‑149 (MQ=255)
           tCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAg                                                                                                                                                     <  2:181703/138‑1 (MQ=255)
           tCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAg                                                                                                                                                     >  1:181703/1‑138 (MQ=255)
           tCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTttat                                                                                                                                           >  7:169259/1‑148 (MQ=255)
               cGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTattatt                                                                                                                                       <  6:371543/148‑1 (MQ=255)
                ggCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTg                                                                                                                                      <  4:244895/148‑1 (MQ=255)
                ggCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGt                                                                                                                                     <  7:470030/149‑1 (MQ=255)
                 gCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGtt                                                                                                                                    <  2:283310/149‑1 (MQ=255)
                 gCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGt                                                                                                                                     >  2:439627/1‑148 (MQ=255)
                  ctcGCTGACGTTGGTTTATGGTATCGAGCCGATGATCGGCGCACTGTTGACAAGCGACAGCTTCTTATTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTa                                                                                                                                   <  6:113426/147‑1 (MQ=255)
                   ccGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAg                                                                                                                                  >  4:401112/1‑149 (MQ=255)
                         aCGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCaaa                                                                                                                              <  3:101311/147‑1 (MQ=255)
                         aCGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAgt                                                                                                                            <  8:467264/149‑1 (MQ=255)
                            ttGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta                                                                                                                                            <  5:276061/130‑1 (MQ=255)
                            ttGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTtta                                                                                                                                            >  6:276061/1‑130 (MQ=255)
                               gTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCt                                                                                                                       <  6:371569/148‑1 (MQ=255)
                                   aTGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGtt                                                                                                                  >  5:168858/1‑149 (MQ=255)
                                   aTGGCATCCCGCCGATCATCGGCGCACTATTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAAGGAATAAAGGGAGCGAAAGCTCCATTTATTATTGTTAGCAAAGTGTGATTCGt                                                                                                                   <  2:263358/148‑1 (MQ=255)
                                    tGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGtt                                                                                                                  <  1:346158/148‑1 (MQ=255)
                                     ggCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTc                                                                                                                                                  >  2:229725/1‑115 (MQ=255)
                                     ggCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTc                                                                                                                                                  <  1:229725/115‑1 (MQ=255)
                                      gCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAt                                                                                                               >  4:361393/1‑149 (MQ=255)
                                       cATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCAtt                                                                                                              <  8:496148/149‑1 (MQ=255)
                                        aTCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTc                                                                                                             <  6:6751/149‑1 (MQ=255)
                                         tCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTcc                                                                                                            >  8:24174/1‑149 (MQ=255)
                                           ccgccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCt                                                                                                           <  3:238213/148‑1 (MQ=255)
                                           ccgccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCt                                                                                                           >  8:272701/1‑148 (MQ=255)
                                             gccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGaa                                                                                                        <  3:309947/149‑1 (MQ=255)
                                             gccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGa                                                                                                         >  8:397289/1‑148 (MQ=255)
                                              ccgATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGaa                                                                                                        >  8:183392/1‑148 (MQ=255)
                                                gATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGt                                                                                                                   >  3:5953/1‑135 (MQ=255)
                                                    atcGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGt                                                                                                                                     >  1:42822/1‑113 (MQ=255)
                                                       ggCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTa                                                                                               <  4:273059/148‑1 (MQ=255)
                                                           cACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAAtt                                                                                          <  7:183392/149‑1 (MQ=255)
                                                             cTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCa                                                                                        <  7:342369/149‑1 (MQ=255)
                                                              tGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                       >  6:20989/1‑149 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAACTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                       <  5:375881/147‑1 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaaa                                                                                            <  6:416975/142‑1 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaaa                                                                                            >  5:416975/1‑142 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                       <  8:169259/147‑1 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCaa                                                                                       <  2:383741/147‑1 (MQ=255)
                                                                ttGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAata                                                                                     <  2:266234/149‑1 (MQ=255)
                                                                    cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAtt                                                                                 >  1:260133/1‑149 (MQ=255)
                                                                    cAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAtt                                                                                 >  2:399072/1‑149 (MQ=255)
                                                                          ccAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtg                                                                           <  6:307613/149‑1 (MQ=255)
                                                                                 cttcttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAgtggggtgg                                                                     <  6:242838/148‑1 (MQ=255)
                                                                                     ttAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCAt                                                                 >  8:179739/1‑148 (MQ=255)
                                                                                       aaTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGa                                                               <  6:48925/148‑1 (MQ=255)
                                                                                       aaTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGAAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGAATGa                                                               >  8:33877/1‑148 (MQ=255)
                                                                                        aTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGAt                                                              <  3:361393/148‑1 (MQ=255)
                                                                                        aTCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATa                                                             <  3:401112/149‑1 (MQ=255)
                                                                                         tCAGCCTGTTGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGTAGCGAAAGCTCCCTTTATTATTGTTAGCTAAGTGTGCTTCGTTCATTCCTTAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATaa                                                            <  1:109466/149‑1 (MQ=255)
                                                                                         tCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaa                                                                                             >  4:416333/1‑116 (MQ=255)
                                                                                         tCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTaaa                                                                                             <  3:416333/116‑1 (MQ=255)
                                                                                         tCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGAt                                                              <  4:379524/147‑1 (MQ=255)
                                                                                         tCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAATGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATaa                                                            <  3:108933/149‑1 (MQ=255)
                                                                                           aGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAg                                                           <  7:179739/148‑1 (MQ=255)
                                                                                           aGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGa                                                          >  2:431684/1‑149 (MQ=255)
                                                                                           aGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGa                                                          <  8:335932/149‑1 (MQ=255)
                                                                                            gCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGAc                                                         <  1:431684/149‑1 (MQ=255)
                                                                                              cTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGAcgc                                                       <  6:88296/149‑1 (MQ=255)
                                                                                                   gCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGa                                                               <  2:180959/136‑1 (MQ=255)
                                                                                                   gCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCa                                                   <  7:338378/148‑1 (MQ=255)
                                                                                                     tGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGc                                                                   <  6:199597/130‑1 (MQ=255)
                                                                                                       ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTc                                              <  1:439627/149‑1 (MQ=255)
                                                                                                       ttAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTc                                              <  1:399072/149‑1 (MQ=255)
                                                                                                          aTGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTAATATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCa                                           >  6:304182/1‑149 (MQ=255)
                                                                                                           tGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCAt                                          >  4:73520/1‑149 (MQ=255)
                                                                                                                aaaTCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGc                                     <  7:397289/149‑1 (MQ=255)
                                                                                                                   tCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTTTTAGCAAAGTGTGCTTCGATCATTCCTGAAAAATAAATAAAAATCAATATATTGCAGTGGGGTCGGCATGATAAGACGCGCCAGCGTCGCATCAGGCaaa                                  <  5:324723/149‑1 (MQ=255)
                                                                                                                     gTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAg                                 <  7:272701/148‑1 (MQ=255)
                                                                                                                      tAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGAc                               <  8:37822/149‑1 (MQ=255)
                                                                                                                          cGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAt                           >  7:5313/1‑149 (MQ=255)
                                                                                                                                aaCGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCACCAGCGTCGCATCAGGCAAAGACAAATCTCGc                      <  5:20989/148‑1 (MQ=255)
                                                                                                                                 aataaTAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCt                    >  7:327273/4‑149 (MQ=255)
                                                                                                                                 aCGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCt                    >  3:367115/1‑149 (MQ=255)
                                                                                                                                    aaTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCa                                                                              <  7:68099/88‑1 (MQ=255)
                                                                                                                                    aaTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGAc                 <  7:33877/149‑1 (MQ=255)
                                                                                                                                     aTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACt                <  2:9950/149‑1 (MQ=255)
                                                                                                                                     aTAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAAGATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGCCAAATCTCGCCTgccg                >  5:271781/1‑146 (MQ=255)
                                                                                                                                      tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAtctc                        <  2:101550/140‑1 (MQ=255)
                                                                                                                                      tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAtctc                        >  1:101550/1‑140 (MQ=255)
                                                                                                                                      tAAAGGGAGCTTTCGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACt                >  5:66796/1‑148 (MQ=255)
                                                                                                                                      tAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACt                >  7:415684/1‑148 (MQ=255)
                                                                                                                                         aGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTAcc            <  7:24174/149‑1 (MQ=255)
                                                                                                                                          gggAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACct           >  1:393576/1‑149 (MQ=255)
                                                                                                                                          gggAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGACATGATAAGACGCGCCAGCGTCGAATCAGGCAAAGACAACTCTCGACTGACTTACct           >  4:421720/1‑149 (MQ=255)
                                                                                                                                                gAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTtcttct     >  1:152461/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ACTGGACCAGATCTTCGGCCCGCTGACGTTGGTTTATGGCATCCCGCCGATCATCGGCGCACTGTTGCCAAGCGCCAGCTTCTTCTTAATCAGCCTGTGGCTGTTAATGAGAAAATCGTAACCGAATGAACGAATAAAGGGAGCGAAAGCTCCCTTTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTTCTCCC  >  NC_000913/4488280‑4488575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: