Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 751,117 T→C 5.2% P351P (CCA→CCG ybgQ ← putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913751,1170TC 5.2% 304.2 / 4.8 97P351P (CCA→CCGybgQputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (46/45);  new base C (3/2);  total (50/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01

TATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAG  >  NC_000913/750969‑751260
                                                                                                                                                    |                                                                                                                                               
tatTTTGCCCGATACCCATTGCGGCAGTATGGTAGTCATCATCAGGAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTGAGTTTGATGTGACATGGAAGGCCGt                                                                                                                                                 <  8:111631/149‑1 (MQ=255)
tatTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGt                                                                                                                                                 <  6:180341/149‑1 (MQ=255)
        ccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGAc                                                                                                                                          <  7:290438/148‑1 (MQ=255)
        ccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGAc                                                                                                                                          >  8:290438/1‑148 (MQ=255)
        ccGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCgggtcagc                                                                                                                                         >  6:461533/1‑143 (MQ=255)
           aTACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCcgc                                                                                                                                      <  4:282076/149‑1 (MQ=255)
               ccATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGc                                                                                                                                   >  1:210528/1‑148 (MQ=255)
               ccATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGc                                                                                                                                   >  3:246112/1‑148 (MQ=255)
                 aTTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCaa                                                                                                                                >  4:130432/1‑149 (MQ=255)
                 aTTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCaa                                                                                                                                <  7:367145/149‑1 (MQ=255)
                 aTTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGcctt                                                                                                                                <  5:23329/149‑3 (MQ=255)
                 aTTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGCGGGTCAGCCACGGcctt                                                                                                                                <  5:461533/149‑3 (MQ=255)
                  ttGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAAt                                                                                                                               >  2:367352/1‑149 (MQ=255)
                       gCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                          <  4:263116/149‑1 (MQ=255)
                       gCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                          <  4:246112/149‑1 (MQ=255)
                       gCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                          <  7:440829/149‑1 (MQ=255)
                        cAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGGTTGATGTGCCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAg                                                                                                                         >  6:265598/1‑149 (MQ=255)
                        cAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTCCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAg                                                                                                                         <  4:422904/149‑1 (MQ=255)
                             tGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAAc                                                                                                                    <  3:130432/149‑1 (MQ=255)
                               gTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCt                                                                                                                  >  8:275623/1‑149 (MQ=255)
                                tAGTCATCATCAGAACTCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGGGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTg                                                                                                                 <  3:127824/149‑1 (MQ=255)
                                   tcatcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTcc                                                                                                              <  2:210528/149‑1 (MQ=255)
                                    cagcatcaGAAATCAGCACGCCGCCGTACCGCGAACTGTTAGACAGAATCCCCCAGGAAACTACAATGCTAAAAAAGGTTTCATTTTCAGATTGATGTGACATCCAAGGCCGTGCCAGACCCGCGGCCAAATTATAGCTAACCTGTcc                                                                                                              >  4:192163/4‑148 (MQ=255)
                                     atcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTccc                                                                                                             <  6:413029/148‑1 (MQ=255)
                                     atcatcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCt                                                                                                            >  7:161250/1‑149 (MQ=255)
                                      tcatcaGAAATCAGCAGGCCGCAGTACAGCGAGGTGTTTGAGAGCATCCCCCAGTAAACTTCATTTCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTg                                                                                                           <  2:275986/149‑1 (MQ=255)
                                       catcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtat                                                                                                                           <  8:134755/132‑1 (MQ=255)
                                       catcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtat                                                                                                                           >  7:134755/1‑132 (MQ=255)
                                        atcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGc                                                                                                         <  7:384607/149‑1 (MQ=255)
                                         tcaGAAATCAGTAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATAGCTAAAAAAGGTTTCATTTTCAGTTTGAGGTGACATGGAAGGCCGAGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGc                                                                                                         >  4:396105/1‑148 (MQ=255)
                                         tcaGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGc                                                                                                         >  2:218752/1‑148 (MQ=255)
                                           aGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGATAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGa                                                                                                       <  5:119383/148‑1 (MQ=255)
                                           aGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGa                                                                                                       <  5:297487/148‑1 (MQ=255)
                                           aGAAATCAGCAGGCCGACGTTCTGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGTCATGGAAGTCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGa                                                                                                       <  4:169997/148‑1 (MQ=255)
                                               aTCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAg                                                                                                  >  4:184015/1‑149 (MQ=255)
                                                   gcagGCCGCCGTACAGCGAGGTGTGTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAg                                                                                              <  8:415608/149‑1 (MQ=255)
                                                    cagGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAgg                                                                                             <  7:34688/149‑1 (MQ=255)
                                                      ggCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGc                                                                                           <  6:4429/149‑1 (MQ=255)
                                                       gccgccGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                          >  2:261662/1‑117 (MQ=255)
                                                       gccgccGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTtata                                                                                                                          <  1:261662/117‑1 (MQ=255)
                                                        ccgccgTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGt                                                                                         <  8:161250/149‑1 (MQ=255)
                                                               aCAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgct                                                                                  >  8:313168/1‑149 (MQ=255)
                                                                cAGCGAGGTGTTTGAGAGCATCACCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtgct                                                                                  <  7:189427/148‑1 (MQ=255)
                                                                  gCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGcc                                                                               <  1:367352/149‑1 (MQ=255)
                                                                       gtgtTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAAc                                                                          >  1:110756/1‑149 (MQ=255)
                                                                          tttGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCATTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTg                                                                       <  6:276070/149‑1 (MQ=255)
                                                                          tttGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGACAGGAAGGGCGTCGATGCTGCCGAAACCTg                                                                       >  2:95250/1‑149 (MQ=255)
                                                                          tttGACATCATCCCCCAGGAAACTTAATTTGTAAAAAAGGTTTCATTTTGAGTTTTATGTGACATGGAAGGCCGTGTCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAAACt                                                                        <  4:129644/148‑1 (MQ=255)
                                                                            tGAGAGCATCCCCCAGGAAACTTCATTTCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTCACCCGCGGCCAATTTATAGCGAACCTGTACCTGGCGAGTCAGGAAGGGCTTCGATGCTGCCGAAACCTgg                                                                      >  3:76142/1‑148 (MQ=255)
                                                                             gagaGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGa                                                                     >  3:118004/1‑148 (MQ=255)
                                                                                agCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAAtt                                                                 >  1:129551/1‑149 (MQ=255)
                                                                                   aTCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGtt                                                              >  7:457839/1‑149 (MQ=255)
                                                                                    tCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTc                                                             <  7:275623/149‑1 (MQ=255)
                                                                                      ccccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCa                                                            <  2:129551/148‑1 (MQ=255)
                                                                                       cccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGCTGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAcc                                                          <  5:265598/149‑1 (MQ=255)
                                                                                        ccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGtt                                                              <  2:304852/144‑1 (MQ=255)
                                                                                        ccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCAccc                                                         >  5:273501/1‑149 (MQ=255)
                                                                                        ccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGAGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGtt                                                              >  1:304852/1‑144 (MQ=255)
                                                                                          aGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGCAAATTGTTCACCCGa                                                       <  3:184015/149‑1 (MQ=255)
                                                                                           ggAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGAc                                                      <  7:313168/149‑1 (MQ=255)
                                                                                                 ttCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGCCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCAtctt                                                >  8:265116/1‑149 (MQ=255)
                                                                                                      tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTcc                                           >  2:362392/1‑149 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGTCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtg                                                                                    >  4:407208/1‑105 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGTCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGAtg                                                                                    <  3:407208/105‑1 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                         >  7:124071/1‑148 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                         >  2:489171/1‑148 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                         >  3:232310/1‑148 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                         >  7:18537/1‑148 (MQ=255)
                                                                                                         tAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTc                                        >  7:382793/1‑149 (MQ=255)
                                                                                                           aaaaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGt                                         <  2:110756/146‑1 (MQ=255)
                                                                                                           aaaaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCAc                                      >  7:87790/1‑149 (MQ=255)
                                                                                                           aaaaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCAc                                      <  1:95250/149‑1 (MQ=255)
                                                                                                             aaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACtt                                    >  1:139898/1‑149 (MQ=255)
                                                                                                             aaaGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACATGGAAATTGTTCACCCGACCATCTTCTTCCGTCACtt                                    >  6:139328/1‑149 (MQ=255)
                                                                                                                ggTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGa                                 <  8:87790/149‑1 (MQ=255)
                                                                                                                  tttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACa                               >  7:359521/1‑149 (MQ=255)
                                                                                                                   ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTg                                                                                                           >  8:461950/1‑72 (MQ=255)
                                                                                                                   ttCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTg                                                                                                           <  7:461950/72‑1 (MQ=255)
                                                                                                                    tCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATc                             >  8:164869/1‑149 (MQ=255)
                                                                                                                      aTTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCa                           >  2:229568/1‑149 (MQ=255)
                                                                                                                       ttttCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATcttctt                                             >  5:432910/1‑130 (MQ=255)
                                                                                                                       ttttCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATcttctt                                             <  6:432910/130‑1 (MQ=255)
                                                                                                                             gTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGcc                    >  2:196973/1‑149 (MQ=255)
                                                                                                                                tGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCt                  <  1:489171/148‑1 (MQ=255)
                                                                                                                                  aTGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa               <  8:382793/149‑1 (MQ=255)
                                                                                                                                    gtgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa               >  1:22664/1‑147 (MQ=255)
                                                                                                                                     tgACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGaa               >  6:447842/1‑146 (MQ=255)
                                                                                                                                      gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa           >  3:275337/1‑149 (MQ=255)
                                                                                                                                      gACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGAGCGTCGATGCTGCCAAAACCTGGAAATTGTTCACACGACAATCTTCTACCGTCACTTTGAAATCAAGCATGCCCTCACCAGa           >  6:209517/1‑149 (MQ=255)
                                                                                                                                       aCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGa           <  1:362392/148‑1 (MQ=255)
                                                                                                                                       aCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGAc          >  8:492457/1‑149 (MQ=255)
                                                                                                                                       aCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACATGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGAc          <  5:139328/149‑1 (MQ=255)
                                                                                                                                        cATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACt         >  8:341487/1‑149 (MQ=255)
                                                                                                                                          tGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGCCCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGa       >  7:435055/1‑149 (MQ=255)
                                                                                                                                            attggCCGCGGGTCAGCCACGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCATCTTCCGGCACTTTGACATCCAGCGTGCCCTGAACAGACTGAtt     >  6:281561/4‑149 (MQ=255)
                                                                                                                                             ttggCCGCGGGTCAGCCACGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGAtt     >  3:190026/3‑148 (MQ=255)
                                                                                                                                              aGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGGCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGa   <  8:124071/149‑1 (MQ=255)
                                                                                                                                               ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAg  <  7:341487/149‑1 (MQ=255)
                                                                                                                                               ggCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGGCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAg  <  3:396105/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
TATTTTGCCCGATACCCATTGCGGCAGAATGGTAGTCATCATCAGAAATCAGCAGGCCGCCGTACAGCGAGGTGTTTGAGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAG  >  NC_000913/750969‑751260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: