Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,123 G→C 8.5% V73L (GTG→CTG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,1230GC 8.5% 335.2 / 15.6 103V73L (GTG→CTG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (47/47);  new base C (6/3);  total (53/50)
Fisher's exact test for biased strand distribution p-value = 4.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCA  >  NC_000913/755976‑756271
                                                                                                                                                   |                                                                                                                                                    
cTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGt                                                                                                                                                     <  5:440965/149‑1 (MQ=255)
    aaaTTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGaaa                                                                                                                                                 <  7:9212/149‑1 (MQ=255)
       tttCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAggg                                                                                                                                              >  3:145272/1‑149 (MQ=255)
         tCCCAGAGCGGCCAGTCCTGTGCGCTGCTCTCTAACGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAgggg                                                                                                                                             <  2:188385/148‑2 (MQ=255)
         tCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGt                                                                                                                                             <  1:474626/148‑1 (MQ=255)
          cccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTc                                                                                                                                            <  2:129201/148‑1 (MQ=255)
           cgagagCGGCCAGACCTGTGCGGTGCTCGCTACAGTCTTCCCGACCCGTTCCCATAGCGTTTCTGCGCAAGTCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATATCTGGGAATGGCATATGTACGACACCGTGAAAGGGTCg                                                                                                                                           <  5:74508/146‑1 (MQ=255)
           ccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCg                                                                                                                                           <  3:359380/148‑1 (MQ=255)
           ccAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCg                                                                                                                                           <  1:310508/148‑1 (MQ=255)
            cAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                                                         <  1:503504/149‑1 (MQ=255)
            cAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTACGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                                                         >  8:248782/1‑149 (MQ=255)
             agagCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGAc                                                                                                                                        >  3:436886/1‑149 (MQ=255)
                   gCCAGACCTGTGCGCTGCTTTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                                                                                                  >  3:212861/1‑149 (MQ=255)
                   gCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATc                                                                                                                                  <  4:430693/149‑1 (MQ=255)
                    ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                                                         >  8:448922/1‑141 (MQ=255)
                    ccAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGa                                                                                                                                         <  7:448922/141‑1 (MQ=255)
                       gACCTGTGCGCTGCTTTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTg                                                                                                                              <  4:212861/149‑1 (MQ=255)
                             tgCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                         >  2:340319/1‑148 (MQ=255)
                              gcgcTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGa                                                                                                                       >  4:97566/1‑149 (MQ=255)
                                  tgctCTCTAAAGTCTTCCCAACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGGAATACCCATGAAGATAACTGGGAAAGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCTGTGACCAGGAcgc                                                                                                                    >  4:49894/1‑148 (MQ=255)
                                       tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGAtt                                                                                                                <  5:233219/147‑1 (MQ=255)
                                       tctAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTg                                                                                                               >  4:264135/1‑148 (MQ=255)
                                         tAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGa                                                                                                              <  8:123612/147‑1 (MQ=255)
                                          aaaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata                                                                                                           >  6:22234/1‑149 (MQ=255)
                                           aaGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGGTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGCCACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAata                                                                                                           >  5:36959/1‑148 (MQ=255)
                                             gTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTACGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatatat                                                                                                        <  7:248782/149‑1 (MQ=255)
                                                ttCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAatata                                                                                                         <  1:323348/145‑1 (MQ=255)
                                                   ccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgt                                                                                                      <  1:229986/145‑1 (MQ=255)
                                                   ccGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgt                                                                                                      >  2:229986/1‑145 (MQ=255)
                                                       cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgt                                                                                                      >  5:107274/1‑141 (MQ=255)
                                                       cccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATAtgt                                                                                                      <  6:107274/141‑1 (MQ=255)
                                                        ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg                                                                                             >  8:378829/1‑149 (MQ=255)
                                                        ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg                                                                                             >  2:235467/1‑149 (MQ=255)
                                                        ccGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCg                                                                                             >  2:264552/1‑149 (MQ=255)
                                                         cGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCgg                                                                                            <  3:97566/149‑1 (MQ=255)
                                                             cccTTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGcc                                                                                         <  4:145272/148‑1 (MQ=255)
                                                               cATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCgg                                                                                       >  3:424379/1‑148 (MQ=255)
                                                                aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCg                                                                                                                                 >  2:49860/1‑105 (MQ=255)
                                                                aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCg                                                                                                                                 <  1:49860/105‑1 (MQ=255)
                                                                aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                         <  5:33748/113‑1 (MQ=255)
                                                                aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAg                                                                                                                         >  6:33748/1‑113 (MQ=255)
                                                                aTACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCgg                                                                                       >  3:375842/1‑147 (MQ=255)
                                                                 tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGTGTAATACCCATGAAGATAACTGGGAATTGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                          <  5:49114/143‑1 (MQ=255)
                                                                 tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAg                                                                                    >  7:389823/1‑149 (MQ=255)
                                                                 tACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATCGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                          >  6:49114/1‑143 (MQ=255)
                                                                   ccGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                   <  3:264135/148‑1 (MQ=255)
                                                                   ccGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGc                                                                                   <  6:314549/148‑1 (MQ=255)
                                                                    cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGCCACCGTGAAAGGGTCGGCCTATATCGGGGACCAGGCCGCGATTGAATATATGTGGACAACCGGGCCGGAAGCGa                                                                                 >  7:126456/1‑149 (MQ=255)
                                                                    cGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                 >  6:422086/1‑149 (MQ=255)
                                                                      tttCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAtt                                                                               >  5:201192/1‑149 (MQ=255)
                                                                            cgcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCAGGAAGCGATTCTGGaa                                                                         <  1:235467/149‑1 (MQ=255)
                                                                             gcAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGGCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAAc                                                                        >  4:311921/1‑149 (MQ=255)
                                                                               aaGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     <  2:433161/134‑1 (MQ=255)
                                                                               aaGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     >  1:433161/1‑134 (MQ=255)
                                                                               aaGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     >  1:439923/1‑134 (MQ=255)
                                                                               aaGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     <  2:439923/134‑1 (MQ=255)
                                                                                aGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTc                                                                      >  6:74445/1‑148 (MQ=255)
                                                                                  gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     <  1:412649/131‑1 (MQ=255)
                                                                                  gcggcATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGaa                                                                                     >  2:412649/1‑131 (MQ=255)
                                                                                  gcggcATTACATGTGCGCTGTGTAATACCCATGAAGATAACTGGGAATGGCATTTGTACGCCACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATCTATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGaa                                                                   <  3:49894/149‑1 (MQ=255)
                                                                                       aTTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACAt                                                              >  5:3306/1‑149 (MQ=255)
                                                                                          aCCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                                            <  1:340319/148‑1 (MQ=255)
                                                                                          aCCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATgg                                                            <  4:375842/148‑1 (MQ=255)
                                                                                           ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                                          <  1:234848/149‑1 (MQ=255)
                                                                                           ccGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGc                                                          >  1:262483/1‑149 (MQ=255)
                                                                                              ttGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                 <  2:417603/123‑1 (MQ=255)
                                                                                              ttGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGa                                                                                 >  1:417603/1‑123 (MQ=255)
                                                                                                  gcTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGt                                                   >  6:181567/1‑149 (MQ=255)
                                                                                                           tACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtc                                          >  2:232682/1‑149 (MQ=255)
                                                                                                           tACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtc                                          >  7:506274/1‑149 (MQ=255)
                                                                                                             cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                        <  4:436886/149‑1 (MQ=255)
                                                                                                             cccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtct                                         <  7:378829/148‑1 (MQ=255)
                                                                                                              ccATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGtctc                                        >  8:244378/1‑148 (MQ=255)
                                                                                                               cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                           >  7:215595/1‑144 (MQ=255)
                                                                                                               cATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGt                                           <  8:215595/144‑1 (MQ=255)
                                                                                                               cATGAAGAAAACGGGGAATGGCAGATGTACGCCACCGTGCAAGGGTCGGACTATATCGGTGACCAGGCCGCGATTGAATAAAGGTGTAAAACCGGGCCGGAAGCGAGTCTGGAACTCGAACCCCTGGGCCTGCCGTGCTCGCGtcgc                                        >  2:15493/1‑145 (MQ=255)
                                                                                                                    aGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 >  4:444998/1‑149 (MQ=255)
                                                                                                                    aGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 <  5:422086/149‑1 (MQ=255)
                                                                                                                    aGATAACTGGGAATGGCATATGTACGACACCCTGAAAGGGTGGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 >  6:384184/1‑149 (MQ=255)
                                                                                                                    aGATAACTGGGAATGGCATATGTACGACACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 <  6:36959/149‑1 (MQ=255)
                                                                                                                      aTAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGt                               <  2:166127/149‑1 (MQ=255)
                                                                                                                       tAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTc                              >  7:72149/1‑149 (MQ=255)
                                                                                                                           tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtat                          >  8:386709/1‑149 (MQ=255)
                                                                                                                           tGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGAATCTGGAACTCGAACACATGGGCCTGCCGGTCTCGCGTCTCGATGATGGTCGtat                          >  2:357245/1‑149 (MQ=255)
                                                                                                                             ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                          >  3:17476/1‑83 (MQ=255)
                                                                                                                             ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGc                                                                                          <  4:17476/83‑1 (MQ=255)
                                                                                                                             ggAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtat                          <  1:264552/147‑1 (MQ=255)
                                                                                                                              gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGtatcta                       >  4:238737/1‑149 (MQ=255)
                                                                                                                              gAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGGCGtatcta                       <  3:311921/149‑1 (MQ=255)
                                                                                                                                   gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 >  6:472823/1‑134 (MQ=255)
                                                                                                                                   gCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGatgatg                                 <  5:472823/134‑1 (MQ=255)
                                                                                                                                     atatGTACGACACCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGt                >  4:362781/1‑149 (MQ=255)
                                                                                                                                         gTACGACACCGTGAAAGGGTCGGACTATATCGGTGCCCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGACCTCGAACAAATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt            >  2:126902/1‑149 (MQ=255)
                                                                                                                                          tACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGt            <  5:22234/148‑1 (MQ=255)
                                                                                                                                            cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGttt          <  6:201192/148‑1 (MQ=255)
                                                                                                                                            cGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTg         <  5:181567/149‑1 (MQ=255)
                                                                                                                                             gACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGGCTCGATGATGGTCGTATCTATCAACGTCCGTTTgg        <  6:3306/149‑1 (MQ=255)
                                                                                                                                              acacCCTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGc       <  2:262483/149‑1 (MQ=255)
                                                                                                                                                 ccGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCCACGTCCGTTTGGCGGt    <  3:238737/149‑1 (MQ=255)
                                                                                                                                                   gTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGCAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCa  <  5:27315/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGCAAATTTCCCAGAGCGGCCAGACCTGTGCGCTGCTCTCTAAAGTCTTCCCGACCCGTTCCCATACCGTTTCTGCGCAAGGCGGCATTACCGTTGCGCTGGGTAATACCCATGAAGATAACTGGGAATGGCATATGTACGACACCGTGAAAGGGTCGGACTATATCGGTGACCAGGACGCGATTGAATATATGTGTAAAACCGGGCCGGAAGCGATTCTGGAACTCGAACACATGGGCCTGCCGTTCTCGCGTCTCGATGATGGTCGTATCTATCAACGTCCGTTTGGCGGTCA  >  NC_000913/755976‑756271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: