Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,172,650 | G→A | 7.0% | R439C (CGT→TGT) | mfd ← | transcription‑repair coupling factor |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,172,650 | 0 | G | A | 7.0% | 173.8 / 3.2 | 58 | R439C (CGT→TGT) | mfd | transcription‑repair coupling factor |
Reads supporting (aligned to +/- strand): ref base G (32/21); new base A (2/2); total (34/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 |
TCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTC > NC_000913/1172505‑1172797 | tCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCAc > 5:164217/1‑149 (MQ=255) cAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCg > 1:189951/1‑149 (MQ=255) ggACCACCGGCTGACCAATATGCAGTTCCGCAAGGCAACGGATCACTGTATCGCGCTTGATGGTGCGGCGAGACTCCTGACGACGACGGGCACCGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACAGTATct > 7:242575/1‑148 (MQ=255) ccGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACaaaa > 6:212971/1‑149 (MQ=255) cTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCAt < 5:212971/149‑1 (MQ=255) aCCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATg < 6:164217/147‑1 (MQ=255) gCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAgcg > 2:384344/1‑149 (MQ=255) cGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatca > 6:113252/1‑149 (MQ=255) cGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACACACCGTATCGACAAAACCATGTTCGGCAGCGCCAatca > 2:339840/1‑149 (MQ=255) cGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACACACCGTATCGACAAAACCATGTTCGGCAGCGCCAatc > 2:339835/1‑148 (MQ=255) gCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatcat > 7:322877/1‑149 (MQ=255) aaGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatcat > 1:455968/1‑147 (MQ=255) aGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAg < 2:224366/149‑1 (MQ=255) tCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatcatc < 1:67409/137‑1 (MQ=255) tCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatcatc > 2:67409/1‑137 (MQ=255) tATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCg > 6:434635/1‑149 (MQ=255) tCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCt < 5:434635/149‑1 (MQ=255) tCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCt > 8:461628/1‑149 (MQ=255) gggTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGc < 2:455968/149‑1 (MQ=255) gggTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGc < 7:461628/149‑1 (MQ=255) ttGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACACACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCtgc < 1:339840/149‑3 (MQ=255) ttGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACACACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTtc < 1:339835/149‑1 (MQ=255) tGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAatcatc < 4:255598/119‑1 (MQ=255) tGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGccc > 2:78244/1‑130 (MQ=255) tGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGccc < 1:78244/130‑1 (MQ=255) tGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTtcatc > 3:34367/1‑148 (MQ=255) tGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTtcatc > 5:478613/1‑148 (MQ=255) cggcgAGAATCCTGACGACGACGGGCAACGCGTTCGCCGAGCAGATCGCTTGCGCAAAGAAGCGCCAGATTACGCACAGTAGCGACAAAACCAGGTTCGGCAGCGCCAATCATCAGATAACCCCCGAGGTCGCTGGCATCATCAAGCCg > 6:302764/1‑149 (MQ=255) gcgAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATGAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCGAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCa < 2:189951/149‑1 (MQ=255) gcgAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCa > 5:270766/1‑149 (MQ=255) gagaATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCAt > 2:438038/1‑148 (MQ=255) cTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGtt < 6:270766/149‑1 (MQ=255) gacgacgacGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGc > 7:413045/1‑149 (MQ=255) acgacgGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAg > 4:197910/1‑149 (MQ=255) gggCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAAtt > 7:445564/1‑149 (MQ=255) gCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAAtttt < 3:197910/149‑1 (MQ=255) gCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAAttt > 1:12694/1‑148 (MQ=255) cAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAAtttt > 4:272439/1‑148 (MQ=255) aaCGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTaa < 1:304164/149‑1 (MQ=255) gcgTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTc < 3:272439/149‑1 (MQ=255) gTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGt < 8:322877/149‑1 (MQ=255) gTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGt < 6:478613/149‑1 (MQ=255) cACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGc < 1:384344/148‑1 (MQ=255) cACCGAGCAGAGAGCTTTCGCAAATCAGCGCCAGATTACTCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGc < 2:367763/148‑1 (MQ=255) gAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCa < 2:12694/149‑1 (MQ=255) gAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCa < 5:113252/149‑1 (MQ=255) aGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAg > 1:149043/1‑149 (MQ=255) gATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCAACGGTCGCTGGCTTAATCAAGACGCATAATTCGATGCGGAGCAATTTTAATTAGTGCGAGCAGTTCa > 6:261401/1‑149 (MQ=255) tCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCAcc > 7:124285/1‑149 (MQ=255) cGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCAccc > 6:365259/1‑149 (MQ=255) cTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCGGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAg > 6:60813/1‑149 (MQ=255) aaaTCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCa > 6:413756/1‑149 (MQ=255) aTCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACg < 8:413045/149‑1 (MQ=255) aTCAGCGCCAGATTACGCACCGTAACGACAAAACCATGTTCGGCAGCGCCAATCAACAGATAACGCCCACGCTCGCTGGCTTCATCAAGACGCAAAATGCGATGCTGAGCAATTTTAATTCGAGCGAGCAGTTc > 7:486985/1‑134 (MQ=255) tCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAAAGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACgg < 5:365259/149‑1 (MQ=255) ccAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCt > 8:369835/1‑149 (MQ=255) cAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCtt > 7:153492/1‑149 (MQ=255) cAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCtt > 5:294897/1‑149 (MQ=255) gCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTc > 7:147856/1‑148 (MQ=255) | TCCAGATGGACCACCGGCTGACCAATATGCAGTTCCGCAAGGTTACGGATCAGTGTATCGGGGTTGATGGTGCGGCGAGAATCCTGACGACGACGGGCAACGCGTTCACCGAGCAGATCGCTTTCGCAAATCAGCGCCAGATTACGCACCGTATCGACAAAACCATGTTCGGCAGCGCCAATCATCAGATAACGCCCACGGTCGCTGGCTTCATCAAGACGCATAATGCGTTGCGGAGCAATTTTAATTCGTGCGAGCAGTTCACCCAGCGCTTCACGGCGACCTTCACTTTC > NC_000913/1172505‑1172797 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |