Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,438,536 A→C 9.6% L750R (CTG→CGG)  pfo ← pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,438,5360AC 9.6% 134.8 / 7.5 52L750R (CTG→CGG) pfopyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (24/23);  new base C (3/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGAATAGCTGAG  >  NC_000913/1438389‑1438682
                                                                                                                                                   |                                                                                                                                                  
tCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAg                                                                                                                                                   >  8:86007/1‑149 (MQ=255)
     tttGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATTGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGTGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTAc                                                                                                                                              <  4:308572/149‑1 (MQ=255)
         cTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAAcc                                                                                                                                          >  5:151122/1‑149 (MQ=255)
          tACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGAGTTTCTCTTCGTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAGTCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCg                                                                                                                                         >  4:332225/1‑149 (MQ=255)
            cGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGt                                                                                                                                       >  7:342248/1‑149 (MQ=255)
             ggTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTg                                                                                                                                      <  8:332019/149‑1 (MQ=255)
             ggTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTg                                                                                                                                      >  5:287105/1‑149 (MQ=255)
                      cTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCTGGCGAGAATTCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGTCGCAGAGGTTATAACCGGTGCAATCttt                                                                                                                              <  4:165291/148‑2 (MQ=255)
                         gaaGGTTCAGGAAGAAATCGTAATTGATTAACACTTCTTATACATGTTACAGGCGAGACATCATATTCATGGCTTTAATCTCTGTATTATGAATGTCTATCGCCGCGCAAACTTCGACGCACACGTTACAACCAGTGCAAAATTccag                                                                                                                           >  3:165291/3‑146 (MQ=255)
                         gCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTTGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGt                                                                                                                          <  6:448856/149‑1 (MQ=255)
                           aGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACCGGTTGTAACCTGTGcgtcga                                                                                                                                <  5:11557/141‑6 (MQ=255)
                           aGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACCGGTTGTAACCTGTGcgtcga                                                                                                                                >  6:11557/1‑136 (MQ=255)
                             gTTGATTAAGAAATCGGAATTGATTTTCTCTTCTTCGACATGTTCCTGGCGAGAAATCATTTTGATGGCTTTAATCTCTGGATTCTGACGGTTTTTGGCCGGGCAAACTTCGATGCACAGGTTAGAACCGGTGCAATCTTCCTGTGc                                                                                                                        <  8:497415/147‑1 (MQ=255)
                              ttGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCg                                                                                                                                         <  3:184316/129‑1 (MQ=255)
                              ttGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCg                                                                                                                                         >  4:184316/1‑129 (MQ=255)
                              ttGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCAc                                                                                                                     <  6:287105/149‑1 (MQ=255)
                                 aGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTg                                                                                                                  >  7:241893/1‑149 (MQ=255)
                                  ggAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGc                                                                                                                 <  6:151122/149‑1 (MQ=255)
                                   gaagaaATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGc                                                                                                                 >  8:88885/1‑148 (MQ=255)
                                      gaaATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGa                                                                                                             <  7:88885/149‑1 (MQ=255)
                                       aaaTCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGAc                                                                                                            <  7:72501/149‑1 (MQ=255)
                                               aTTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACCGGTTGTAACCTGt                                                                                                                                       >  6:375382/1‑114 (MQ=255)
                                               aTTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACCGGTTGTAACCTGt                                                                                                                                       <  5:375382/114‑1 (MQ=255)
                                                           ttcttcGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCat                                                                                          <  7:92803/147‑1 (MQ=255)
                                                             cttcTACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCata                                                                                         <  4:49674/146‑1 (MQ=255)
                                                             cttcGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATc                                                                                       <  8:342248/148‑1 (MQ=255)
                                                             cttcGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCa                                                                                      >  1:174052/1‑149 (MQ=255)
                                                                cGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCAc                                                                                     <  5:223773/147‑1 (MQ=255)
                                                                      gTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGAttt                                                                              >  7:126735/1‑148 (MQ=255)
                                                                       ttCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTc                                                                             <  7:86007/148‑1 (MQ=255)
                                                                          cAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACAt                                                                         >  8:354608/1‑149 (MQ=255)
                                                                             gCGAGAGATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGGCCGCGCATATCACGCGATTTCACATCCa                                                                      <  8:246007/149‑1 (MQ=255)
                                                                                    atcatATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATg                                                               <  7:354608/149‑1 (MQ=255)
                                                                                       atatTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACTACCGGTGCATTCTTCCGGTGCCACCTGCAAGAGATATTTCTGCCCGCGCATATCACGCGATTTCATATCCAGCGAATGCa                                                             <  2:291931/148‑1 (MQ=255)
                                                                                            gATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTg                                                        <  7:232561/148‑1 (MQ=255)
                                                                                              tGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTgg                                                       >  3:276860/1‑147 (MQ=255)
                                                                                                gCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAgg                                                   >  6:319957/1‑149 (MQ=255)
                                                                                                  tttAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAgggg                                                 >  5:380795/1‑149 (MQ=255)
                                                                                                  tttAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAgggg                                                 >  7:110022/1‑149 (MQ=255)
                                                                                                     aaTCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGt                                              >  8:413069/1‑149 (MQ=255)
                                                                                                         tctGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACCACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTc                                          >  2:450464/1‑149 (MQ=255)
                                                                                                         tctGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCCTATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTc                                          >  1:361633/1‑149 (MQ=255)
                                                                                                           tGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCa                                        >  3:191276/1‑149 (MQ=255)
                                                                                                               ttCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGc                                    <  1:211606/149‑1 (MQ=255)
                                                                                                                   gACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCtt                                  >  7:188386/1‑147 (MQ=255)
                                                                                                                                cGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACtt                   <  5:426017/149‑1 (MQ=255)
                                                                                                                                  ggCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACtttt                 <  5:319957/149‑1 (MQ=255)
                                                                                                                                         ttCGCCGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCCCCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGaa          >  6:112671/1‑149 (MQ=255)
                                                                                                                                         ttCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTcc                                         <  4:106537/118‑1 (MQ=255)
                                                                                                                                         ttCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTcc                                         >  3:106537/1‑118 (MQ=255)
                                                                                                                                           gattgCACCGGTTGTAACCTGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGaa          >  3:177294/5‑147 (MQ=255)
                                                                                                                                               gCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGAATAGCt     >  8:43234/1‑148 (MQ=255)
                                                                                                                                                cacaGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGAATAGCTGa   <  4:276860/149‑1 (MQ=255)
                                                                                                                                                 acaGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTgg                                                       <  2:450088/96‑1 (MQ=255)
                                                                                                                                                 acaGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTgg                                                       >  1:450088/1‑96 (MQ=255)
                                                                                                                                                 acaGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGGTTTCCATCGCTTCAGGCGGCACCACTATTGCGCGAAAAGCTGAg  >  2:39263/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCCAGTTTGCTACGGTCGATTTCTGGCAGGTTGAGGAAGAAATCGTAATTGATTTTCTCTTCTTCGACATGTTCCAGGCGAGACATCATATTGATGGCTTTAATCTCTGGATTCTGACGGTCTTTCGCCGGGCAAACTTCGACGCACAGGTTACAACCGGTGCAATCTTCCGGTGCCACCTGCAAGACATATTTCTGCCCGCGCATATCACGCGATTTCACATCCAGCGAATGCAGGCTGGCAGGGGCGTTTTCCATCGCTTCAGGCGGCACCACTTTTGCGCGAATAGCTGAG  >  NC_000913/1438389‑1438682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑