Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,504,888 T→G 5.1% intergenic (+23/+17) insQ → / ← ydcO IS609 transposase B/BenE family inner membrane putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,504,8880TG 5.1% 236.1 / 2.7 78intergenic (+23/+17)insQ/ydcOIS609 transposase B/BenE family inner membrane putative transporter
Reads supporting (aligned to +/- strand):  ref base T (33/41);  new base G (2/2);  total (35/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

GCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCG  >  NC_000913/1504740‑1505035
                                                                                                                                                    |                                                                                                                                                   
gCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCtt                                                                                                                                                     >  1:123408/1‑149 (MQ=255)
gCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCtt                                                                                                                                                     >  2:26133/1‑149 (MQ=255)
gCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAAGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCtt                                                                                                                                                     >  7:6064/1‑149 (MQ=255)
  cGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCa                                                                                                                                                   <  6:42744/149‑1 (MQ=255)
   gATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAg                                                                                                                                                  >  8:477027/1‑149 (MQ=255)
   gATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAAAGATTCAGGCGCCCGCCTGAACGAAGCAGGGATCCACGTCCTTCAg                                                                                                                                                  >  8:454528/1‑149 (MQ=255)
    aTGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAgg                                                                                                                                                 <  3:164474/149‑1 (MQ=255)
         aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGt                                                                                                                                             <  1:123549/148‑1 (MQ=255)
         aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTg                                                                                                                                            >  3:369410/1‑149 (MQ=255)
         aaTGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTg                                                                                                                                            >  6:469365/1‑149 (MQ=255)
           tGGCGCTCGCAACATTTTAGCGGTGGGGGACGCCTTTATTGCATGTGGAGAGATGGTGCAGTCTGGCCGCCCGTTGAAGCAGGAACCCTCCGAAATGTTTCAGGCGACATCCTGTACGTAGCAGGGATCCACGTCCTTCAGGTCGTgga                                                                                                                                          <  6:30537/149‑1 (MQ=255)
             gcgcTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGCCCTGAAGGACGTggat                                                                                                                                         <  6:315534/148‑2 (MQ=255)
              cgcCCGCAACATTTTCGCGGCGGGGCACGCCGGTCTTGCCTGTGGAGCGCTGGCGCAGTCAGGCCGCCCGTTGACGCAGGACCCCCCCGAACTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTggagga                                                                                                                                       <  4:271955/149‑1 (MQ=255)
                cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATg                                                                                                                                     >  1:12709/1‑149 (MQ=255)
                cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATg                                                                                                                                     >  2:175979/1‑149 (MQ=255)
                cTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATg                                                                                                                                     >  5:343686/1‑149 (MQ=255)
                   gCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTc                                                                                                                                   <  1:396820/148‑1 (MQ=255)
                   gCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTc                                                                                                                                   >  7:320397/1‑148 (MQ=255)
                     aaCATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGt                                                                                                                                             <  7:242627/136‑1 (MQ=255)
                     aaCATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGt                                                                                                                                             >  8:242627/1‑136 (MQ=255)
                        aTTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCACCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATc                                                                                                                             >  1:251723/1‑149 (MQ=255)
                         ttttAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCg                                                                                                                            <  8:68018/149‑1 (MQ=255)
                               cggcggGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCt                                                                                                                      <  6:21592/149‑1 (MQ=255)
                                   ggggCACGCCGTTCTTGCCTGTGGAGAGAGGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCa                                                                                                                  <  7:454528/149‑1 (MQ=255)
                                        aCGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGAt                                                                                                                                      >  6:25292/1‑124 (MQ=255)
                                        aCGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGAt                                                                                                                                      <  5:25292/124‑1 (MQ=255)
                                         cGCCGTTCTTGCCTGTGGAGAGATGGTGCTGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGGCTGAACGTAGCAGGGATCCTCGTCCTTCAGGTCGTGGTGGATGGCAATATCGGTTTCTGTCAGCGAtt                                                                                                            <  3:400663/149‑1 (MQ=255)
                                           ccGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTaa                                                                                                          <  8:320397/149‑1 (MQ=255)
                                               tCTTGCCTGTGGAGAGATTGTGCAGTCAGGCCGCCCGTTGAAGCAGGCACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                      <  2:223847/149‑1 (MQ=255)
                                                 ttGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCACGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCaa                                                                                                    <  4:369410/149‑1 (MQ=255)
                                                   gCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAaca                                                                                                  >  2:26190/1‑149 (MQ=255)
                                                        tgGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAAcaccac                                                                                              <  7:326861/148‑1 (MQ=255)
                                                        tgGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGAGTAACTTCAACACCACg                                                                                             >  6:86681/1‑149 (MQ=255)
                                                             agaTGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAAACCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAAc                                                                                         >  6:224639/1‑148 (MQ=255)
                                                                tGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAAc                                                                                     <  5:469365/149‑1 (MQ=255)
                                                                tGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAAc                                                                                     <  7:368838/149‑1 (MQ=255)
                                                                   tGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                                   >  8:223570/1‑148 (MQ=255)
                                                                     cAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTc                                                                                <  1:175979/149‑1 (MQ=255)
                                                                     cAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTc                                                                                <  6:343686/149‑1 (MQ=255)
                                                                       gTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCg                                                                              >  4:151197/1‑149 (MQ=255)
                                                                        tCAGGCCGCCCGTCGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCgg                                                                             >  1:498512/1‑149 (MQ=255)
                                                                          aGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCaa                                                                                                    <  7:309991/124‑1 (MQ=255)
                                                                          aGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCaa                                                                                                    >  8:309991/1‑124 (MQ=255)
                                                                            gccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAgg                                                                                                                                                 >  7:313671/1‑77 (MQ=255)
                                                                            gccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAgg                                                                                                                                                 <  8:313671/77‑1 (MQ=255)
                                                                             ccgccCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAAtt                                                                        <  2:12709/149‑1 (MQ=255)
                                                                                 ccGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATc                                                                    <  7:477027/149‑1 (MQ=255)
                                                                                  cGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATcc                                                                   >  3:146159/1‑149 (MQ=255)
                                                                                    ttGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                                   >  1:353846/1‑131 (MQ=255)
                                                                                    ttGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGc                                                                                   <  2:353846/131‑1 (MQ=255)
                                                                                                    ccGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGTGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAAt                                                 <  1:26133/149‑1 (MQ=255)
                                                                                                     cGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATc                                                <  2:498512/149‑1 (MQ=255)
                                                                                                      gAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCACCACCACGTAACAAACGCCTCCGGCAAGTAATCCCCAAAACGCAGAACCAATcc                                               >  6:417289/1‑149 (MQ=255)
                                                                                                       aaaTGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATccc                                              >  1:107095/1‑149 (MQ=255)
                                                                                                       aaaTGATTCAGGCGACAGCCTGAAAGTAGCAGGGATACCCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTTAATTCAACACCACGTAACAAACGCCTCAGGCAATTAATCCACAAAACGCAGAACCAATccc                                              >  7:385431/1‑149 (MQ=255)
                                                                                                           gATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCgcc                                           >  2:486979/1‑146 (MQ=255)
                                                                                                             ttCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAACCGCAGACCCAATCCCGCCCAg                                        >  6:71640/1‑149 (MQ=255)
                                                                                                                aGGCGACAGCCTGAACGTAGGAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAAGACCACGTAACTAACGCCTCCGGCAATTAATCCCCATAACGGAGAACCTATCCCGACCAGCGt                                     <  6:307658/149‑1 (MQ=255)
                                                                                                                aGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGa                                                       >  2:479517/1‑131 (MQ=255)
                                                                                                                aGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGa                                                       <  1:479517/131‑1 (MQ=255)
                                                                                                                    gACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTTACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAAt                                 <  8:6064/149‑1 (MQ=255)
                                                                                                                    gACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAAt                                 <  7:223570/149‑1 (MQ=255)
                                                                                                                    gACAGCCTGAACGTAGCAGGGATCCACGTCATTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGACTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAAt                                 <  4:13361/149‑1 (MQ=255)
                                                                                                                              aCGTAGCAGGGATCCACGTCATTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCg                       <  2:123408/149‑1 (MQ=255)
                                                                                                                               cGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGTTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGt                      <  1:190329/149‑1 (MQ=255)
                                                                                                                                 tAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTa                    >  4:454974/1‑149 (MQ=255)
                                                                                                                                   gacatcctCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTAc                   >  6:302986/8‑148 (MQ=255)
                                                                                                                                   gCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTAc                   <  2:107095/148‑1 (MQ=255)
                                                                                                                                    cAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCa                 <  3:151197/149‑1 (MQ=255)
                                                                                                                                     catcctCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAg                <  5:302986/144‑1 (MQ=255)
                                                                                                                                     aGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTAc                   >  6:415831/1‑146 (MQ=255)
                                                                                                                                        gATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGaaa             >  1:58805/1‑149 (MQ=255)
                                                                                                                                         aTCCACGTCCTTCATGGCGTGGAGGATGTCAATATCGGTTGCTGTCAGCGATTAAAATCAACCCCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGACGTTACCAGAAAt            <  8:250144/149‑1 (MQ=255)
                                                                                                                                          tCCACGCCCTGAAGGACGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATg           >  6:356609/1‑149 (MQ=255)
                                                                                                                                            cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGACCCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc          >  6:262941/1‑148 (MQ=255)
                                                                                                                                            cACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGc          <  1:26190/148‑1 (MQ=255)
                                                                                                                                             aCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTg                          <  3:61763/131‑1 (MQ=255)
                                                                                                                                             aCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTg                          >  4:61763/1‑131 (MQ=255)
                                                                                                                                             aCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca        <  3:454974/149‑1 (MQ=255)
                                                                                                                                             aCGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGcca        >  1:240142/1‑149 (MQ=255)
                                                                                                                                                  cTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAACCGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCaccacc   <  4:146159/149‑1 (MQ=255)
                                                                                                                                                   ttCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                      <  1:191447/49‑1 (MQ=255)
                                                                                                                                                   ttCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTc                                                                                                      >  2:191447/1‑49 (MQ=255)
                                                                                                                                                   ttCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCAccg  >  3:442762/1‑149 (MQ=255)
                                                                                                                                                   ttCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCAccg  >  4:300429/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCGATGTAAATGGCGCTCGCAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGAGATGGTGCAGTCAGGCCGCCCGTTGAAGCAGGAACCCACCGAAATGATTCAGGCGACAGCCTGAACGTAGCAGGGATCCACGTCCTTCAGGGCGTGGAGGATGTCAATATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCG  >  NC_000913/1504740‑1505035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: