Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,534,897 A→T 5.3% intergenic (+28/+68) nhoA → / ← yddE N‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,534,8970AT 5.3% 354.0 / 5.7 108intergenic (+28/+68)nhoA/yddEN‑hydroxyarylamine O‑acetyltransferase/PhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base A (49/52);  new base T (2/3);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.58e-01

Only 100 of 108 total aligned reads displayed.

TGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGTTGGTTATCAC  >  NC_000913/1534752‑1535044
                                                                                                                                                 |                                                                                                                                                   
tGCTGTGATGCAAGAACTGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAAc                                                                                                                                                  <  7:2234/149‑1 (MQ=255)
   tgtgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGcc                                                                                                                                               >  1:198821/1‑149 (MQ=255)
     tgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCtt                                                                                                                                             <  3:213471/149‑1 (MQ=255)
     tgATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATAAGGCGTTTACGccgc                                                                                                                                             <  4:325472/149‑3 (MQ=255)
         gCAACAACAGTTTGGTCAGGGCGTGGATGATGAGAAACATGGCTTTACCGTGGATAAGTTAGCGCTCTTGATGGCGACGTTAGATACGAACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCCGATGCCGCGTAAACGCATTATcc                                                                                                                                         >  4:292982/1‑149 (MQ=255)
           atgAACAGTTTGGTCTGGGCTTGGATGATTCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAAc                                                                                                                                                  <  4:248929/136‑1 (MQ=255)
           aaGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAAc                                                                                                                                                  >  3:248929/1‑138 (MQ=255)
           aaGAACAGATTGGTCTGGCCGTGGATGATGCGAAACATGGCTTTACAGTGGATGAGTTAGCGCTTGAGATGGCGGCCTTTGAAACGCACCAGGAGGCGGGAAAATAATTTATGTCAGGTTGCCCGATCCGGCGTAAACGCCTTATCCgg                                                                                                                                       >  8:262973/1‑149 (MQ=255)
             gAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                      >  4:166186/1‑148 (MQ=255)
             gAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                      <  3:166186/148‑1 (MQ=255)
                   tttGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACAt                                                                                                                                >  5:274426/1‑148 (MQ=255)
                     tGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTa                                                                                                                              <  4:8008/148‑1 (MQ=255)
                     tGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTa                                                                                                                              <  5:111999/148‑1 (MQ=255)
                          tGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCgg                                                                                                                        >  2:179572/1‑149 (MQ=255)
                            ggCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCgg                                                                                                                        <  6:222989/147‑1 (MQ=255)
                            ggCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGtt                                                                                                                      <  4:72009/149‑1 (MQ=255)
                               gTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTg                                                                                                                     >  6:199275/1‑147 (MQ=255)
                                  gatgatGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCt                                                                                                                                              >  5:221607/1‑119 (MQ=255)
                                  gatgatGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCt                                                                                                                                              <  6:221607/119‑1 (MQ=255)
                                    tgatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCTGTTGTAGCCCt                                                                                                              >  1:136518/1‑149 (MQ=255)
                                    tgatgCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCt                                                                                                              <  1:179572/149‑1 (MQ=255)
                                         cGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGtt                                                                                                                      <  5:232344/136‑1 (MQ=255)
                                         cGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACa                                                                                                          <  3:209557/148‑1 (MQ=255)
                                         cGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAg                                                                                                         >  6:91437/1‑149 (MQ=255)
                                          gAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAgg                                                                                                        >  1:446161/1‑149 (MQ=255)
                                          gAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAgg                                                                                                        <  5:261933/149‑1 (MQ=255)
                                          gAAACATGGCTTTACAGTTGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGAGGGAAAATAATTTATGTCAGGTTGCCGGATGCGTCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGCCCTGACAgg                                                                                                        >  4:345186/1‑149 (MQ=255)
                                               aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAg                                                                                                   >  4:261485/1‑149 (MQ=255)
                                               aTGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAg                                                                                                   >  4:175982/1‑149 (MQ=255)
                                                    tttACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCa                                                                                               >  5:162528/1‑148 (MQ=255)
                                                      tACCGGGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCAt                                                                                              <  2:68295/147‑1 (MQ=255)
                                                       aCCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCa                                                                                            <  5:324604/148‑1 (MQ=255)
                                                           tGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                       >  7:297742/1‑101 (MQ=255)
                                                           tGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                                                                                       <  8:297742/101‑1 (MQ=255)
                                                           tGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAgg                                                                                                        >  1:171944/1‑132 (MQ=255)
                                                             gATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACg                                                                                     >  3:48050/1‑149 (MQ=255)
                                                                  gTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGttt                                                                                 >  3:50771/1‑148 (MQ=255)
                                                                   ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                               <  4:50771/149‑1 (MQ=255)
                                                                   ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                               <  4:48050/149‑1 (MQ=255)
                                                                   ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                               <  1:52508/149‑1 (MQ=255)
                                                                   ttAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCa                                                                               <  3:175982/149‑1 (MQ=255)
                                                                        gcTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGtt                                                                          <  5:199275/149‑1 (MQ=255)
                                                                          tGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATc                                                                                             >  8:118264/1‑128 (MQ=255)
                                                                          tGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATc                                                                                             <  7:118264/128‑1 (MQ=255)
                                                                             tGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAAtt                                                                     >  2:289847/1‑149 (MQ=255)
                                                                               aTGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTc                                                                                >  2:270999/1‑136 (MQ=255)
                                                                               aTGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTc                                                                                <  1:270999/136‑1 (MQ=255)
                                                                               aTGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGc                                                                   <  2:198821/149‑1 (MQ=255)
                                                                                cggcggcGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGcc                                                                  >  6:396390/2‑149 (MQ=255)
                                                                                  gcggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCGCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAAAGGTTTCAGAGTTCAATTGccc                                                                 <  2:144471/148‑1 (MQ=255)
                                                                                  gcggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCa                                                                >  8:210371/1‑149 (MQ=255)
                                                                                   cggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGcc                                                                  >  7:15921/1‑146 (MQ=255)
                                                                                   cggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGcc                                                                  <  8:15921/146‑1 (MQ=255)
                                                                                    ggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCa                                                                                            >  2:66910/1‑119 (MQ=255)
                                                                                    ggcgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCa                                                                                            <  1:66910/119‑1 (MQ=255)
                                                                                      cgTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCg                                                            <  2:446161/149‑1 (MQ=255)
                                                                                        tttGATACGCACCCGGAGGCGGGAAATTAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCgg                                                           >  4:26979/1‑148 (MQ=255)
                                                                                        tttGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCgg                                                           >  3:387965/1‑148 (MQ=255)
                                                                                            aTACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATg                                                       >  7:210074/1‑148 (MQ=255)
                                                                                             tACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATg                                                       >  2:480716/1‑147 (MQ=255)
                                                                                             tACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATg                                                       <  1:480716/147‑1 (MQ=255)
                                                                                                  aCCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACaaa                                                <  7:210371/149‑1 (MQ=255)
                                                                                                   cccGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACaaaa                                               >  5:479146/1‑149 (MQ=255)
                                                                                                    ccGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACaaaa                                               <  8:319092/148‑1 (MQ=255)
                                                                                                    ccGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACaaaa                                               >  7:319092/1‑148 (MQ=255)
                                                                                                        aGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCAcc                                          <  1:289847/149‑1 (MQ=255)
                                                                                                        aGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCAcc                                          <  8:210074/149‑1 (MQ=255)
                                                                                                            gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGc                                                                                                       <  3:462129/84‑1 (MQ=255)
                                                                                                            gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTGAATTGCCCATTCGGCATGAAACAAAATCACCGCtg                                      >  1:51489/1‑149 (MQ=255)
                                                                                                            gggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCtg                                      >  3:368105/1‑149 (MQ=255)
                                                                                                             ggAAAATAATTTATGTCAGGTTGCGGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCtgt                                     <  8:227076/149‑1 (MQ=255)
                                                                                                             ggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCtgt                                     >  5:85862/1‑149 (MQ=255)
                                                                                                             ggAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGATCAATTGCCCATTCGGCATGAAACAAAATCACCGCtgt                                     <  6:274426/149‑1 (MQ=255)
                                                                                                               aaaaTAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGc                                   >  1:461228/1‑149 (MQ=255)
                                                                                                               aaaaTAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGc                                   <  6:162528/149‑1 (MQ=255)
                                                                                                                  ataatTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTg                                >  3:4969/1‑148 (MQ=255)
                                                                                                                     atTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGaaa                             <  3:261485/149‑1 (MQ=255)
                                                                                                                      tttATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAAt                            <  6:85862/149‑1 (MQ=255)
                                                                                                                      tttATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAAt                            >  5:467980/1‑149 (MQ=255)
                                                                                                                       ttATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGAc                                                                                                                <  8:168258/64‑1 (MQ=255)
                                                                                                                       ttATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGAc                                                                                                                >  7:168258/1‑64 (MQ=255)
                                                                                                                          tGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGt                         <  2:461228/148‑1 (MQ=255)
                                                                                                                          tGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGt                         <  4:368105/148‑1 (MQ=255)
                                                                                                                          tGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGt                         >  8:146450/1‑148 (MQ=255)
                                                                                                                            tCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGa                       >  3:317554/1‑148 (MQ=255)
                                                                                                                            tCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGa                       <  4:317554/148‑1 (MQ=255)
                                                                                                                            tCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGAc                      <  5:396390/149‑1 (MQ=255)
                                                                                                                                gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGaa                              >  5:194269/1‑137 (MQ=255)
                                                                                                                                gTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGaa                              <  6:194269/137‑1 (MQ=255)
                                                                                                                                     cGGATGCGGCGTAAACGCCTTATCCGGCATACAGTAGCCGGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCTGAGTTGAATTGCCCATTCGGCATGAAACGAAGGCGCGGCTGTGGCTGGAAGGGTGGCtgttgcggg             >  2:348498/1‑144 (MQ=255)
                                                                                                                                     cGGATAAGGCGTTTACGCCGCATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGt                         <  3:140728/137‑1 (MQ=255)
                                                                                                                                     cGGATAAGGCGTTTACGCCGCATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGt                         >  4:140728/1‑137 (MQ=255)
                                                                                                                                      ggATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggt            <  4:170400/149‑1 (MQ=255)
                                                                                                                                      ggATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggt            <  6:298685/149‑1 (MQ=255)
                                                                                                                                       gataaggcgTTTACGCCGCATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggtt           >  5:123513/6‑149 (MQ=255)
                                                                                                                                        aTGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggttg          <  6:467980/149‑1 (MQ=255)
                                                                                                                                         taaggcgTTTACGCCGCATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggttgg         <  6:123513/146‑1 (MQ=255)
                                                                                                                                           cggcgTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCggttggtt       <  5:91437/149‑1 (MQ=255)
                                                                                                                                                tAAACGCCTTATCCGTCATACATTAGCCCGGTTTTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGTTGGTTATCAc  <  3:345186/149‑1 (MQ=255)
                                                                                                                                                tAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGTTGGTTATCAc  >  6:391595/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGCTGTGATGCAAGAACAGTTTGGTCTGGGCGTGGATGATGCGAAACATGGCTTTACCGTGGATGAGTTAGCGCTGGTGATGGCGGCGTTTGATACGCACCCGGAGGCGGGAAAATAATTTATGTCAGGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCATACATTAGCCCGGTTGTAGACCTGACAGGCGAAGCGCATCAGACAACGGTTTCAGAGTTCAATTGCCCATTCGGCATGAAACAAAATCACCGCTGTGCCTGAAATGGTGACTTTTTCCGGTTGGTTATCAC  >  NC_000913/1534752‑1535044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: