Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,595,637 G→A 5.3% pseudogene (2351/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,595,6370GA 5.3% 239.9 / 2.6 76pseudogene (2351/3861 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base G (34/37);  new base A (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

ATATATCCCTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGC  >  NC_000913/1595490‑1595780
                                                                                                                                                   |                                                                                                                                               
atatatCCCTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAgcg                                                                                                                                                 >  3:371379/1‑148 (MQ=255)
  atatCCCTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAg                                                                                                                                              >  1:134979/1‑149 (MQ=255)
     tCCCTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCg                                                                                                                                            <  6:269349/148‑1 (MQ=255)
        cTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCACAGCGgtgt                                                                                                                                        >  3:241901/1‑149 (MQ=255)
            gcgAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTc                                                                                                                                    <  8:393217/149‑1 (MQ=255)
                           ggAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAATCGCAGCGGTGTTGTCGCGTTCCAGAGTGaa                                                                                                                     <  3:143283/149‑1 (MQ=255)
                            gAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAg                                                                                                                    <  2:134979/149‑1 (MQ=255)
                               tatCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTa                                                                                                                 <  6:35683/149‑1 (MQ=255)
                               tatCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTa                                                                                                                 <  7:100271/149‑1 (MQ=255)
                                  ccGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTg                                                                                                              >  2:499314/1‑149 (MQ=255)
                                   cGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGt                                                                                                             <  3:204074/149‑1 (MQ=255)
                                       tCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCttt                                                                                                         >  4:104891/1‑149 (MQ=255)
                                         gAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCa                                                                                                       <  4:294424/149‑1 (MQ=255)
                                          aaaaTGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAg                                                                                                      >  2:265325/1‑149 (MQ=255)
                                             atgatgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTg                                                                                                   >  7:126271/1‑149 (MQ=255)
                                              tgatgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCACAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTgg                                                                                                  <  4:241901/149‑1 (MQ=255)
                                                atgGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGc                                                                                                <  3:104891/149‑1 (MQ=255)
                                                   gTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCaa                                                                                              <  4:298125/148‑1 (MQ=255)
                                                     aCCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAacac                                                                                           >  5:183222/1‑149 (MQ=255)
                                                       cTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCg                                                                                         <  8:126271/149‑1 (MQ=255)
                                                        tCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCgg                                                                                        >  4:81100/1‑149 (MQ=255)
                                                            ttCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGaa                                                                                    <  5:479688/149‑1 (MQ=255)
                                                            ttCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGaa                                                                                    <  1:28986/149‑1 (MQ=255)
                                                               aTGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAAtt                                                                                  <  4:371379/148‑1 (MQ=255)
                                                                 ggCCCGTCCACCAATGGATTGCTCTCCAACTTTTACCGAGGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAg                                                                               <  5:459324/149‑1 (MQ=255)
                                                                       tCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTg                                                                         >  6:114473/1‑149 (MQ=255)
                                                                       tCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTg                                                                         <  2:267179/149‑1 (MQ=255)
                                                                         caccaATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGtt                                                                       <  3:81100/149‑1 (MQ=255)
                                                                             aaTGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGAAACATCGCGTGAGTAAGCTCAGCGGTGTTGTCGCGTTCAAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCACTGTTGCAt                                                                   >  3:43604/1‑149 (MQ=255)
                                                                               tGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATgg                                                                 <  7:167195/149‑1 (MQ=255)
                                                                               tGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATgg                                                                 <  1:265325/149‑1 (MQ=255)
                                                                                 gATTGCTCTCCTACTTTTACCGATGTGGTATTTTCACTGTCTTACTGCATCATCGCGTGAGTATGCGCAGCTGTGTTGTCGCGTTCCAGAGTGAATGTACTGTGTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCTTggg                                                                <  3:420315/148‑1 (MQ=255)
                                                                                  aTTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCg                                                                                         <  5:358532/122‑1 (MQ=255)
                                                                                  aTTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCg                                                                                         >  6:358532/1‑122 (MQ=255)
                                                                                   ttGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTaa                                                             <  5:63186/149‑1 (MQ=255)
                                                                                   ttGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCACAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGaa                                                                                    <  6:54426/126‑1 (MQ=255)
                                                                                   ttGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCACAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGaa                                                                                    >  5:54426/1‑126 (MQ=255)
                                                                                       tctcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGc                                                          >  3:229894/1‑148 (MQ=255)
                                                                                         tcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCttt                                                                                                         >  8:435160/1‑99 (MQ=255)
                                                                                         tcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCttt                                                                                                         <  7:435160/99‑1 (MQ=255)
                                                                                         tcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTcc                                                                            >  7:491243/1‑128 (MQ=255)
                                                                                         tcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTcc                                                                            <  8:491243/128‑1 (MQ=255)
                                                                                         tcCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCa                                                        >  2:220951/1‑148 (MQ=255)
                                                                                           cAACTTTTACCGATGTTGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCAATAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGATGCATGGGGAAAGCCaaa                                                      >  2:400763/1‑148 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCGACATCGCGTGAGTAAGCGCAGCGGTGTTGTcgc                                                                                                                                  <  4:420492/72‑1 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCGACATCGCGTGAGTAAGCGCAGCGGTGTTGTcgc                                                                                                                                  >  3:420492/1‑72 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTggg                                                                                                 <  8:179635/105‑1 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTggg                                                                                                 >  7:179635/1‑105 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCaaa                                                      <  6:183222/148‑1 (MQ=255)
                                                                                           cAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCaaa                                                      >  2:76067/1‑148 (MQ=255)
                                                                                            aaCTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACa                                                    >  1:247379/1‑149 (MQ=255)
                                                                                              cTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGCAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATc                                                  <  6:118344/149‑1 (MQ=255)
                                                                                               ttttACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCt                                                 >  5:249092/1‑149 (MQ=255)
                                                                                                    ccGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTc                                             <  8:108322/148‑1 (MQ=255)
                                                                                                    ccGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCg                                            <  1:76067/149‑1 (MQ=255)
                                                                                                          tgGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGAt                                      >  5:297064/1‑149 (MQ=255)
                                                                                                            gTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGa                                    >  6:329299/1‑149 (MQ=255)
                                                                                                                tttCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGc                                >  7:498277/1‑149 (MQ=255)
                                                                                                                 ttCACTGTCAGACTGCAACTTCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCt                               >  6:52460/1‑149 (MQ=255)
                                                                                                                  tCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCt                               <  6:249092/148‑1 (MQ=255)
                                                                                                                  tCACTGTAATACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTGAGTTTGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCt                               <  1:400763/148‑1 (MQ=255)
                                                                                                                    aCTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCa                            >  3:299498/1‑149 (MQ=255)
                                                                                                                       gTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTc                         >  6:251627/1‑149 (MQ=255)
                                                                                                                        tCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTc                         <  5:329299/148‑1 (MQ=255)
                                                                                                                        tCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCg                        >  3:360733/1‑149 (MQ=255)
                                                                                                                          aGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCa                      >  1:421581/1‑149 (MQ=255)
                                                                                                                              tGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATc                    >  1:448784/1‑147 (MQ=255)
                                                                                                                              tGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATc                    >  4:430140/1‑147 (MQ=255)
                                                                                                                              tGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATc                    >  3:10297/1‑147 (MQ=255)
                                                                                                                                cAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCaa                  <  1:499314/147‑1 (MQ=255)
                                                                                                                                cAACATCGCGGGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTAGGCCAACACCGGCGAATTCAGTCCCAGTTGCATGGGTAAAGCCAACCATCATGTAGGACGATGCCAGCTGCACTCGCATCACGc                >  7:502627/1‑149 (MQ=255)
                                                                                                                                    aTCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATc            <  5:114473/149‑1 (MQ=255)
                                                                                                                                     tCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGACGGAGGATGACAGCTGCACTCGCATCAAGCCATCg           >  5:182719/1‑149 (MQ=255)
                                                                                                                                         gTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCtt       >  2:441811/1‑149 (MQ=255)
                                                                                                                                          tGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCttt      <  3:430140/149‑1 (MQ=255)
                                                                                                                                          tGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCttt      <  1:220951/149‑1 (MQ=255)
                                                                                                                                              tAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAgc  >  2:124808/1‑149 (MQ=255)
                                                                                                                                                 gcgcAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCggagg                                        >  2:420171/1‑108 (MQ=255)
                                                                                                                                                 gcgcAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCggagg                                        <  1:420171/108‑1 (MQ=255)
                                                                                                                                                   gcAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCaa                  <  7:30272/128‑1 (MQ=255)
                                                                                                                                                   gcAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCaa                  >  8:30272/1‑128 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
ATATATCCCTCCGCGAGCGTCGCAGCAGGAATATCCGTATCGAAAATGATGGTACCTCCATTCATGGCCAGTCCACCAATGGATTGCTCTCCAACTTTTACCGATGTGGTATTTTCACTGTCAGACTGCAACATCGCGTGAGTAAGCGCAGCGGTGTTGTCGCGTTCCAGAGTGAAGGTACTGTCTTTCAGTTGGGCAACACCGGCGAATTCAGTCCCTGTTGCATGGGTAAAGCCAAACATCTTGTCGGAGGATGACAGCTGCACTCGCATCAAGCCATCGCCTTTGAGC  >  NC_000913/1595490‑1595780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: