Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,727,776 T→G 5.6% intergenic (+20/‑61) nemA → / → gloA chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme/glyoxalase I, Ni‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,727,7760TG 5.6% 296.0 / 3.7 95intergenic (+20/‑61)nemA/gloAchromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme/glyoxalase I, Ni‑dependent
Reads supporting (aligned to +/- strand):  ref base T (52/37);  new base G (2/4);  total (54/41)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTG  >  NC_000913/1727629‑1727923
                                                                                                                                                   |                                                                                                                                                   
gTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGt                                                                                                                                                     <  2:129125/148‑1 (MQ=255)
          gATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAgccgcc                                                                                                                                            <  3:399358/147‑1 (MQ=255)
            ttGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCCCAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCta                                                                                                                                        >  5:261651/1‑149 (MQ=255)
             tcatgcccagcactttggtataaaaatcgatggagcgttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGTAAAGCCGCCGCta                                                                                                                                        <  2:119966‑M1/21‑1 (MQ=255)
               cGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCta                                                                                                                                        <  3:278266/146‑1 (MQ=255)
               cGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCta                                                                                                                                        >  4:278266/1‑146 (MQ=255)
               cGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATAc                                                                                                                                     >  3:435108/1‑149 (MQ=255)
               cGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATAc                                                                                                                                     >  7:392669/1‑149 (MQ=255)
               atgcccagcactttggtataaaaatcgatggagcgttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGTAAAGCCGCCGCTATAc                                                                                                                                     >  1:119966‑M1/126‑149 (MQ=255)
                gAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATAc                                                                                                                                     >  4:60434/1‑148 (MQ=255)
                 aaCCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTaaa                                                                                                                                                  >  8:381534/1‑134 (MQ=255)
                     cGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTaaaa                                                                                                                                >  7:98298/1‑148 (MQ=255)
                       gATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCTTGCAGAAAGATTCTACGGTGGAGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATACAACATTGCGAGCGGAGTACAGCCGCCGCTATACAAAaaca                                                                                                                              >  8:301377/1‑148 (MQ=255)
                            ggTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCACCGCTATACTAAAACATCAttt                                                                                                                        >  3:454531/1‑149 (MQ=255)
                             gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGTCGTAAAGCCACCGCTATACTAAAACAACAtttt                                                                                                                       <  4:454531/149‑1 (MQ=255)
                             gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACAtttt                                                                                                                       <  6:98970/149‑1 (MQ=255)
                             gTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGGGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAacaaca                                                                                                                           <  6:261651/145‑1 (MQ=255)
                               cGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGa                                                                                                                     <  8:392669/149‑1 (MQ=255)
                                            cgcAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAt                                                                                                        <  3:169552/149‑1 (MQ=255)
                                             gcAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCAtt                                                                                                       >  7:308638/1‑149 (MQ=255)
                                                 gttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGGCTTTACGCCGCTATACTAAAACAACATTTTGAATCTGTTa                                                                                                             >  2:152295‑M1/92‑139 (MQ=255)
                                                 gttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGGCTTTACGCCGCTATACTAAAACAACATTTTGAATCTGTTa                                                                                                             >  2:158942‑M1/92‑139 (MQ=255)
                                                 gttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGGCTTTACGCCGCTATACTAAAACAACATTTTGAATCTGTTa                                                                                                             <  1:158942‑M1/48‑1 (MQ=255)
                                                 gttgcaaatcgccaacgcgcagcatggtatgaagaagacgcatctttttatcctcaaaatggctaacagattcaaaatgttgttttagtatAGCGGCGGCTTTACGCCGCTATACTAAAACAACATTTTGAATCTGTTa                                                                                                             <  1:152295‑M1/48‑1 (MQ=255)
                                                  gCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTCGCCATTTTGAg                                                                                                  >  6:320501/1‑149 (MQ=255)
                                                   cTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAgg                                                                                                 <  6:486614/149‑1 (MQ=255)
                                                    tGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGa                                                                                                >  7:372945/1‑149 (MQ=255)
                                                     gAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGa                                                                                                >  5:91396/1‑148 (MQ=255)
                                                      aGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATa                                                                                              <  2:328840/149‑1 (MQ=255)
                                                             cccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGAt                                                                                       <  5:440099/149‑1 (MQ=255)
                                                             cccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGAt                                                                                       <  4:389078/149‑1 (MQ=255)
                                                             cccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGATGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGAt                                                                                       <  6:290856/149‑1 (MQ=255)
                                                              ccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACAttt                                                                                                                        >  6:306201/1‑115 (MQ=255)
                                                              ccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACAttt                                                                                                                        <  5:306201/115‑1 (MQ=255)
                                                              ccACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATg                                                                                      >  3:386213/1‑149 (MQ=255)
                                                                acaGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAacaaca                                                                                                                           <  6:35218/110‑1 (MQ=255)
                                                                acaGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAacaaca                                                                                                                           >  5:35218/1‑110 (MQ=255)
                                                                 caGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGt                                                                                   >  4:328474/1‑149 (MQ=255)
                                                                  aGCGGGCCGAACGTTTCTACGGTGGCGGCGCGGCAGGCTATCCCGATTACCCGACGGTGTCATCCAACCTTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtc                                                                                  <  4:381839/149‑1 (MQ=255)
                                                                     gagCCGAAAGTTTCTACGCTGGCGGCGCGGAAGGCTAAACCCATTACCCGACGTTGTAAACCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGtcta                                                                                >  4:187394/3‑147 (MQ=255)
                                                                         cGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAg                                                                                                  >  2:454294/1‑126 (MQ=255)
                                                                         cGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAg                                                                                                  <  1:454294/126‑1 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAacaaca                                                                                                                           <  2:442253/100‑1 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAacaaca                                                                                                                           >  1:442253/1‑100 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCa                                                                           >  1:14944/1‑148 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCa                                                                           >  4:418445/1‑148 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCa                                                                           >  8:344764/1‑148 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCa                                                                           >  7:451879/1‑148 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCa                                                                           >  6:39475/1‑148 (MQ=255)
                                                                          gAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCAt                                                                          <  3:60434/149‑1 (MQ=255)
                                                                           aaaGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGAt                                                                                       <  6:456422/135‑1 (MQ=255)
                                                                           aaaGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGAt                                                                                       >  5:456422/1‑135 (MQ=255)
                                                                           aaaGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATa                                                                         >  4:14706/1‑149 (MQ=255)
                                                                                  cTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCAtgct                                                                  >  2:313118/1‑149 (MQ=255)
                                                                                      ggTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTgcgc                                                              <  8:451879/149‑1 (MQ=255)
                                                                                       gTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCCACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATTCTGcgcg                                                             >  8:447697/1‑149 (MQ=255)
                                                                                          gcggcgCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTg                                                          >  5:209685/1‑149 (MQ=255)
                                                                                            ggcgCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGc                                                        <  3:14706/149‑1 (MQ=255)
                                                                                               gcgGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGAt                                                     <  8:308638/149‑1 (MQ=255)
                                                                                                 ggAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGAttt                                                   <  3:328474/149‑1 (MQ=255)
                                                                                                  gAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTg                                                  <  4:386213/149‑1 (MQ=255)
                                                                                                   aaGGCTATACCGATTACCCGACGTTGTAATCCAACATTGTGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGAt                                                     >  1:340752/1‑145 (MQ=255)
                                                                                                   aaGGCTATACCGATTACCCGACGTTGTAATCCAACATTGTGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGAt                                                     <  2:340752/145‑1 (MQ=255)
                                                                                                        tataCCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGc                                            >  2:444140/1‑149 (MQ=255)
                                                                                                        tataCCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGACTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGc                                            >  4:80899/1‑149 (MQ=255)
                                                                                                           aCCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTc                                          >  4:110911/1‑148 (MQ=255)
                                                                                                            ccGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTcc                                         <  2:14944/148‑1 (MQ=255)
                                                                                                               aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGc                                                                                     <  7:28313/101‑1 (MQ=255)
                                                                                                               aTTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGc                                                                                     >  8:28313/1‑101 (MQ=255)
                                                                                                                ttACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGATCCATCGa                                    >  7:500622/1‑149 (MQ=255)
                                                                                                                   cccGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAATCAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTAATACCCTCCTGCGCGTTGGCGAGTTGCAACGCTCAATCGAttt                                 >  6:364694/1‑149 (MQ=255)
                                                                                                                   cccGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACCACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATTCCATGCTGCGCGTTGGCGATTTGAAACGCTCCATCGAttt                                 >  8:223910/1‑149 (MQ=255)
                                                                                                                   cccGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGAttt                                 <  3:418445/149‑1 (MQ=255)
                                                                                                                   cccGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGAttt                                 >  5:268007/1‑149 (MQ=255)
                                                                                                                      gACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTta                              >  1:86433/1‑149 (MQ=255)
                                                                                                                      gACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTta                              >  5:237290/1‑149 (MQ=255)
                                                                                                                      attcaaaatgttgttttagtatAGCGGCGGCTTTACGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTta                              <  8:59934‑M1/127‑1 (MQ=255)
                                                                                                                       aCGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTtat                             <  6:237290/149‑1 (MQ=255)
                                                                                                                        cggtGTAATCCACCATTGCGAGCCGCGGAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTACCCATTTTGAGGATAAAAAGCTGCGTCTTCTTCATACCAAGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTtata                            <  6:15142/146‑1 (MQ=255)
                                                                                                                         gTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATAc                           >  1:371256/1‑149 (MQ=255)
                                                                                                                          ttGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATAc                           >  4:267829/1‑148 (MQ=255)
                                                                                                                             tAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCaaa                       <  1:313118/149‑1 (MQ=255)
                                                                                                                              aaTCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAg                      >  8:102521/1‑149 (MQ=255)
                                                                                                                               aTCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGt                     >  3:295733/1‑149 (MQ=255)
                                                                                                                               aTCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGt                     <  8:372945/149‑1 (MQ=255)
                                                                                                                                tCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTg                    >  4:146622/1‑149 (MQ=255)
                                                                                                                                  cAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCt                  <  3:187394/149‑1 (MQ=255)
                                                                                                                                    aCATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTgg                <  3:110911/149‑1 (MQ=255)
                                                                                                                                     cATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTggg               >  1:244840/1‑149 (MQ=255)
                                                                                                                                       ttGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTta                              <  2:179941/132‑1 (MQ=255)
                                                                                                                                       ttGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTta                              >  1:179941/1‑132 (MQ=255)
                                                                                                                                           gAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGa          >  5:35872/1‑148 (MQ=255)
                                                                                                                                             GCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAAc       >  7:102521‑M1/1‑149 (MQ=255)
                                                                                                                                                 CGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAActgct   <  1:484772‑M1/149‑1 (MQ=255)
                                                                                                                                                  GTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAActgctg  <  2:424798‑M1/149‑1 (MQ=255)
                                                                                                                                                  GTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAActgctg  >  5:39475‑M1/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCACAGCGTGCCGAAAGTTTCTACGGTGGCGGCGCGGAAGGCTATACCGATTACCCGACGTTGTAATCCAACATTGCGAGCGGCGTAAAGCCGCCGCTATACTAAAACAACATTTTGAATCTGTTAGCCATTTTGAGGATAAAAAGATGCGTCTTCTTCATACCATGCTGCGCGTTGGCGATTTGCAACGCTCCATCGATTTTTATACCAAAGTGCTGGGCATGAAACTGCTG  >  NC_000913/1727629‑1727923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: