Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,768,131 A→T pseudogene (911/945 nt) rhsJ → Rhs‑family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,768,1310AT99.4% 630.1 / NA 180pseudogene (911/945 nt)rhsJRhs‑family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (89/90);  minor base G (1/0);  total (90/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTC  >  NC_000913/3767986‑3768274
                                                                                                                                                 |                                                                                                                                               
ggAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAgg                                                                                                                                              >  4:365462/1‑149 (MQ=255)
     tAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGc                                                                                                                                         <  4:297398/149‑1 (MQ=255)
     tAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCAATCAGGGCACCCCATTTCTAATATGCTAGGTACGc                                                                                                                                         >  5:24492/1‑149 (MQ=255)
        tGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGc                                                                                                                                         >  4:6969/1‑146 (MQ=255)
        tGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGc                                                                                                                                         >  5:141382/1‑146 (MQ=255)
          gTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaa                                                                                                                                    <  3:144738/149‑1 (MQ=255)
           tAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaa                                                                                                                                   <  5:107825/149‑1 (MQ=255)
            aaGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaa                                                                                                                                   >  5:381272/1‑148 (MQ=255)
             aGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaaa                                                                                                                                  <  8:313280/148‑1 (MQ=255)
              gTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAc                                                                                                                                >  3:16650/1‑149 (MQ=255)
               ttAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATTGCTATAACAACAAAAGACCTTGGTAATTGGTTAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAcc                                                                                                                               <  2:32593/149‑1 (MQ=255)
                          tACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGatat                                                                                                                      >  7:266360/1‑147 (MQ=255)
                           aCAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGatat                                                                                                                      >  4:62446/1‑146 (MQ=255)
                           aCAGTGCTAACAGCACCAACGAACCTTAATTCAAATAACGATCTAATGGATATTACAACAAACGACCTTGTTAATTGGATAACATATCGGCCATCTGGGCACACCAGTTCAAATATGCGAGGTAAGCCGCAAAAAACAAGTGatat                                                                                                                      >  6:369266/1‑146 (MQ=255)
                            cAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACCCCAGAAAAACCCAGTGATATAAGa                                                                                                                  <  8:204113/149‑1 (MQ=255)
                             aGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGa                                                                                                                  >  4:273383/1‑148 (MQ=255)
                                 cTAACAGCACCAACGAACCTTAATTCTAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAgg                                                                                                              <  3:3897/148‑1 (MQ=255)
                                   aaCAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTg                                                                                                           >  2:130605/1‑149 (MQ=255)
                                       gCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGa                                                                                                       <  3:365462/149‑1 (MQ=255)
                                        cACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTCCGCCAGAAAAACCCAGTGCTATAAGATAGGGTGTAGAt                                                                                                      >  3:199682/1‑149 (MQ=255)
                                           cAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAAACCAGTGATATAAGATAGGTTGTAGATGTa                                                                                                   >  3:127883/1‑149 (MQ=255)
                                            aaCGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTAt                                                                                                  <  1:329018/149‑1 (MQ=255)
                                             aCGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATg                                                                                                 <  8:303817/149‑1 (MQ=255)
                                                   cTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTa                                                                                                   <  4:171301/141‑1 (MQ=255)
                                                   cTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCa                                                                                           <  4:60299/149‑1 (MQ=255)
                                                       aTTCAAATAAGTATCTAATGGCTATAACAACAAAAGACCTTGGTAATTTGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGc                                                                                        <  5:364917/148‑1 (MQ=255)
                                                       aTTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGc                                                                                        <  6:217775/148‑1 (MQ=255)
                                                       aTTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCt                                                                                       >  8:204317/1‑149 (MQ=255)
                                                        ttCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGaaaaa                                                                                                                                 >  6:165616/1‑106 (MQ=255)
                                                           aaaTAAGGATCTAATGGCTAAAACAACAAAAGACCTAGCGAATAGGATAACATATGGGCCATCTGGGCACCCCAATTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGt                                                                                    <  2:276135/148‑1 (MQ=255)
                                                             aTAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCAt                                                                                          >  5:185315/1‑140 (MQ=255)
                                                             aTAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTAc                                                                                 >  3:219949/1‑149 (MQ=255)
                                                                  gATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGt                                                                                                                           <  8:105694/102‑1 (MQ=255)
                                                                  gATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGt                                                                                                                           >  7:105694/1‑102 (MQ=255)
                                                                  gATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAttt                                                                            >  8:71586/1‑149 (MQ=255)
                                                                  gATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAttt                                                                            >  2:249378/1‑149 (MQ=255)
                                                                   aTCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCTATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAtttt                                                                           >  8:235993/1‑149 (MQ=255)
                                                                   aTCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTAAGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAtttt                                                                           >  5:22280/1‑149 (MQ=255)
                                                                       aaTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTa                                                                       >  5:25907/1‑149 (MQ=255)
                                                                        aTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAttttt                                                                          <  3:362507/145‑1 (MQ=255)
                                                                        aTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTa                                                                       <  4:16650/148‑1 (MQ=255)
                                                                        aTGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAt                                                                      >  3:308050/1‑149 (MQ=255)
                                                                           gCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAttt                                                                    <  2:204417/148‑1 (MQ=255)
                                                                            cTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATTTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTtct                                                                  >  8:318435/1‑149 (MQ=255)
                                                                             tataACAACAAAAGACCTTGGTAAGTGGATAACATATGGGCCATCTTGGCACCCCATTTCTAATATGCTAGGTACCCCAGAAAAACCAAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTtctc                                                                 <  1:109268/149‑1 (MQ=255)
                                                                               taACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTTATATAATATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtt                                                               <  3:34800/149‑1 (MQ=255)
                                                                                aacaacAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCttt                                                              >  4:307606/1‑149 (MQ=255)
                                                                                 acaacaAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtttt                                                             <  7:321946/149‑1 (MQ=255)
                                                                                  caacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTTGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTctct                                                                <  1:82097/145‑1 (MQ=255)
                                                                                  caacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGc                                                                                            <  1:362971/117‑1 (MQ=255)
                                                                                  caacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTctct                                                                >  2:82097/1‑145 (MQ=255)
                                                                                   aacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGt                                                                                    <  3:68791/124‑1 (MQ=255)
                                                                                   aacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTtct                                                                  <  6:67286/142‑1 (MQ=255)
                                                                                   aacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTtct                                                                  >  5:67286/1‑142 (MQ=255)
                                                                                   aacaaAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTAt                                                           >  3:326223/1‑149 (MQ=255)
                                                                                      aaaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATATGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTg                                                        >  2:41569/1‑149 (MQ=255)
                                                                                       aaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCTATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGt                                                       <  7:235993/149‑1 (MQ=255)
                                                                                       aaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTg                                                        >  4:128400/1‑148 (MQ=255)
                                                                                       aaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGt                                                       <  3:283049/149‑1 (MQ=255)
                                                                                       aaaGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGt                                                       <  5:33868/149‑1 (MQ=255)
                                                                                        aaGACCTTGGTAATTGGATAACATATGGGCCATCTTGGCACCCCATTTCTAATATTCTAGGTACTAAAGAAAAACCCAGTGATATAAGATAGGTTTTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTAtattttattttt                                                       >  4:305325/1‑146 (MQ=255)
                                                                                         aGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCa                                                     <  4:219949/149‑1 (MQ=255)
                                                                                             cTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTctct                                                                >  8:386673/1‑134 (MQ=255)
                                                                                             cTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTctct                                                                <  7:386673/134‑1 (MQ=255)
                                                                                             cTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTg                                                 >  3:196326/1‑149 (MQ=255)
                                                                                              ttGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATGTAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTg                                                 >  3:203119/1‑148 (MQ=255)
                                                                                                ggTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCa                                               <  6:270845/148‑1 (MQ=255)
                                                                                                ggTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCAt                                              >  3:260852/1‑149 (MQ=255)
                                                                                                 gTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATg                                             <  1:146130/149‑1 (MQ=255)
                                                                                                  tAATTGGATACCATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATgg                                            <  6:341827/149‑1 (MQ=255)
                                                                                                  tAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATgg                                            <  1:249378/149‑1 (MQ=255)
                                                                                                   aaTTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTtct                                                                  >  2:370288/1‑126 (MQ=255)
                                                                                                   aaTTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGt                                           >  4:97473/1‑149 (MQ=255)
                                                                                                    aTTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTCACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGt                                           <  6:381272/148‑1 (MQ=255)
                                                                                                     ttGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGc                                         <  7:71586/149‑1 (MQ=255)
                                                                                                        gttAACATATGGGCCATCTGGGCACCCCATTTCGAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGAGAGGTTGTAGATGGATGAAAGCGTGCTTGTGACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGTTGCTGa                                      >  3:19822/3‑149 (MQ=255)
                                                                                                        gATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGa                                      >  7:307917/1‑149 (MQ=255)
                                                                                                        caTAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGa                                      <  1:296908/148‑1 (MQ=255)
                                                                                                           aaCATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTAt                                                                      <  8:36802/114‑1 (MQ=255)
                                                                                                           aaCATATGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGAtgt                                   >  5:160649/1‑149 (MQ=255)
                                                                                                              atatGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCCGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTaaa                                <  2:166776/149‑1 (MQ=255)
                                                                                                                atGGGCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGaaaa                              >  5:117404/1‑149 (MQ=255)
                                                                                                                    gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtt                                                               >  1:109643/1‑112 (MQ=255)
                                                                                                                    gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCtt                                                               <  2:109643/112‑1 (MQ=255)
                                                                                                                    gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCa                                               <  4:114475/128‑1 (MQ=255)
                                                                                                                    gCCATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCa                                               >  3:114475/1‑128 (MQ=255)
                                                                                                                     ccATCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaa                         <  1:25276/149‑1 (MQ=255)
                                                                                                                       aTCTGGGCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTAttt                                                                            <  2:321923/96‑1 (MQ=255)
                                                                                                                            ggCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATaaaacaa                      <  8:266360/145‑1 (MQ=255)
                                                                                                                            ggCACCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACg                  <  6:238390/149‑1 (MQ=255)
                                                                                                                               aCCCCATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGaaa               <  2:192267/149‑1 (MQ=255)
                                                                                                                                  ccATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTg                                          <  1:372206/119‑1 (MQ=255)
                                                                                                                                  ccATTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTa            >  1:205207/1‑149 (MQ=255)
                                                                                                                                    aTTTCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTat           >  4:334849/1‑148 (MQ=255)
                                                                                                                                     tttCTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatga         >  1:181132/1‑149 (MQ=255)
                                                                                                                                        cTAATATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTatgatg       <  4:154509/148‑1 (MQ=255)
                                                                                                                                          aaTATGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATtggg    >  7:171/1‑149 (MQ=255)
                                                                                                                                           atatGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAAt                                                   <  6:281368/101‑1 (MQ=255)
                                                                                                                                           atatGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAAt                                                   >  5:281368/1‑101 (MQ=255)
                                                                                                                                            tatGCTAGGTACGCCAGAAAAACCCAGTGATATAAGATATGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTc  <  1:41569/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGAACTAATGGTAAGTTAAAAATGGATACAGTGCTAACAGCACCAACGAACCTTAATTCAAATAAGGATCTAATGGCTATAACAACAAAAGACCTTGGTAATTGGATAACATATGGGCCATCTGGGCACCCCATTTCTAATATGCAAGGTACGCCAGAAAAACCCAGTGATATAAGATAGGTTGTAGATGTATGAAAGCATGCTTGTTACTATTTTTTTATTTCTCTTTTATTTGTCAATTGCATGGTGCTGATGTGAAAATAAAACAAAACGAAAGTATGATGGGGTC  >  NC_000913/3767986‑3768274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: