Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,721,243 G→A G431R (GGA→AGA)  pka → protein lysine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,721,2430GA98.8% 276.0 / NA 80G431R (GGA→AGA) pkaprotein lysine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (43/36);  minor base T (0/1);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGC  >  NC_000913/2721100‑2721385
                                                                                                                                               |                                                                                                                                              
cgcAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAAcc                                                                                                                                           <  7:26327/149‑1 (MQ=255)
           aTTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCttt                                                                                                                                >  7:367072/1‑149 (MQ=255)
           aTTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCttt                                                                                                                                >  1:201950/1‑149 (MQ=255)
           aTTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCttt                                                                                                                                >  4:320317/1‑149 (MQ=255)
           aTTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCtt                                                                                                                                 <  3:226246/148‑1 (MQ=255)
              gAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTCCCGTACCCCGGAAAGAACCATCACTGCTTTTAt                                                                                                                             >  2:52387/1‑149 (MQ=255)
               aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATg                                                                                                                            <  7:177201/149‑1 (MQ=255)
               aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATg                                                                                                                            <  3:173067/149‑1 (MQ=255)
               aaGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTCGTGCGGCGAGCACTCATCGCAAGAGGCACGACGTTAATTCAGCGAAGCCGGGCTGCCGACCTACAGTACCCAGGAAAGAACCATCACTGCTTTTATg                                                                                                                            >  5:224447/1‑149 (MQ=255)
                       aaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggt                                                                                                                    <  8:58740/149‑1 (MQ=255)
                       aaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggt                                                                                                                    >  7:236361/1‑149 (MQ=255)
                       aaGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggt                                                                                                                    <  1:304433/149‑1 (MQ=255)
                        aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                   <  3:320317/149‑1 (MQ=255)
                        aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                   <  8:367072/149‑1 (MQ=255)
                        aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                   <  2:201950/149‑1 (MQ=255)
                        aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                   <  3:336652/149‑1 (MQ=255)
                        aGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtggtg                                                                                                                   <  5:40607/149‑1 (MQ=255)
                           atcatcCCCGCAGCAAATATGTCTCTTTGCTGAATAACTGGTTCGTCGAGCAATCCTCGCAAGAGTCACGAAGTTTATTCAGCGAAGCCGGGCTGCCGACATACATTACCCCGGAAAGAACCATCAATGCTTTTATGCATATGGTTGAg                                                                                                                >  2:381285/1‑149 (MQ=255)
                                  ccGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGc                                                                                                         >  5:38719/1‑149 (MQ=255)
                                  ccGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGc                                                                                                         >  1:302037/1‑149 (MQ=255)
                                       gcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAAt                                                                                                    <  1:52387/149‑1 (MQ=255)
                                       gcaAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAAt                                                                                                    <  8:330892/149‑1 (MQ=255)
                                           atatGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAg                                                                                                 >  1:5040/1‑148 (MQ=255)
                                                tctcTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGAAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCaa                                                                                           <  2:42880/149‑1 (MQ=255)
                                                  tctTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAAc                                                                                          >  4:315675/1‑148 (MQ=255)
                                                           aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAAc                                                                                >  6:42739/1‑149 (MQ=255)
                                                           aCGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAAc                                                                                <  2:29993/149‑1 (MQ=255)
                                                           aCGAACTGGGGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAAc                                                                                <  6:257473/149‑1 (MQ=255)
                                                                   gTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGc                                                                          <  2:5040/147‑1 (MQ=255)
                                                                         cgAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCa                                                                  >  7:356373/1‑149 (MQ=255)
                                                                               ctcctcGCAAGAGGCACGACGTTTATTCTGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTTCGCGAAACGCCGGCGTTGCCCAGCAATc                                                            <  7:383663/149‑1 (MQ=255)
                                                                               ctcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGCAGCAACTACGCGAAACGCCGGCGTTGCCCCGCAATc                                                            >  1:94265/1‑149 (MQ=255)
                                                                               ctcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATc                                                            <  4:106551/149‑1 (MQ=255)
                                                                                tcctcGCAAGCGGCACGACTTTTATTCATCGAAGCCGGGCTGCCGACATACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATAtgg                                                                                                                     <  8:180639/91‑1 (MQ=255)
                                                                                tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                           >  7:226531/1‑149 (MQ=255)
                                                                                tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                           >  1:47670/1‑149 (MQ=255)
                                                                                tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGATTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCt                                                           >  2:343641/1‑149 (MQ=255)
                                                                                tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACAGTACCACGGAAAGAACCATCACTGCTTTTATGCATAtgg                                                                                                                     >  7:180639/1‑91 (MQ=255)
                                                                                tcctcGCAAGAGGCACGACGTTTATTCAGCGAAGACGGGATGCCGAACTACCGTACCACGCAAAGACCAATAACTCCTTTTATGCATATGGTGGAGTACCGGCGGAATCTGAAGCAACTCCGCGAAACGCCGGAGTTGCCCAGCAATCt                                                           >  7:272062/1‑149 (MQ=255)
                                                                                  ctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGa                                                         >  1:149597/1‑149 (MQ=255)
                                                                                  ctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGa                                                         >  5:114239/1‑149 (MQ=255)
                                                                                  ctcGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAAGCTGa                                                         >  5:117386/1‑149 (MQ=255)
                                                                                     gCAAGAGGCACGCCGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAACCGCCGGCGTTGCCCCGCAATCTGAcgt                                                      >  5:161247/1‑147 (MQ=255)
                                                                                     gCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGAc                                                        <  5:42739/147‑1 (MQ=255)
                                                                                     gCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACtt                                                      <  3:315675/149‑1 (MQ=255)
                                                                                     gCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACt                                                       <  6:38719/148‑1 (MQ=255)
                                                                                         gagGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCaa                                                  >  3:71001/1‑149 (MQ=255)
                                                                                             cACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATAcc                                              >  7:283807/1‑149 (MQ=255)
                                                                                              acgacgTTTATTCAGTGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCAGACTTCCAATACCg                                             >  7:169112/1‑149 (MQ=255)
                                                                                              acgacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGa                                                                                                >  1:10150/1‑98 (MQ=255)
                                                                                              acgacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGa                                                                                                <  2:10150/98‑1 (MQ=255)
                                                                                              acgacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCg                                             <  8:283807/149‑1 (MQ=255)
                                                                                                gacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGTAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCa                                           <  3:105406/149‑1 (MQ=255)
                                                                                                gacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc                                                                   >  8:360471/1‑125 (MQ=255)
                                                                                                gacgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGccc                                                                   <  7:360471/125‑1 (MQ=255)
                                                                                                 acgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAg                                          <  5:339459/149‑1 (MQ=255)
                                                                                                   gTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGAGTTCCAATACCGCAg                                          >  8:334172/1‑147 (MQ=255)
                                                                                                   gTTTATTCAGCGAAGACGTGCTGCAGACCTACCGTACCCCGTAAAGAACCATCACTGCTTTTATGCATATGGTTGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGaa                                        <  1:381285/149‑1 (MQ=255)
                                                                                                      tATGCAGTGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCAGACTTCCAATACCGCAGAAgc                                      <  8:169112/148‑1 (MQ=255)
                                                                                                         tCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCATAAGCAACTACGCGAAACg                                                                               <  4:159869/104‑1 (MQ=255)
                                                                                                         tCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACg                                                                               >  3:159869/1‑104 (MQ=255)
                                                                                                           aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGCACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtct                                >  1:106938/1‑149 (MQ=255)
                                                                                                           aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtct                                >  3:127529/1‑149 (MQ=255)
                                                                                                           aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtct                                >  3:190507/1‑149 (MQ=255)
                                                                                                           aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCCGCGTAATCAGCAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtc                                 >  1:119246/1‑148 (MQ=255)
                                                                                                              gAAGGCGGGCTGCCGACCTACCGTACCCCTGAAAGAACCTTCACTGCTTTTATGGATATGGTGGATTACCGGCGTAATCAGAAGCAACTAAGCGAAACGCCGGCTTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtcttct                             <  5:364228/149‑1 (MQ=255)
                                                                                                              gAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCAtcttct                             >  6:192510/1‑149 (MQ=255)
                                                                                                               aaGCCGGGCTGCCGACCTACAGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTg                            <  8:236361/149‑1 (MQ=255)
                                                                                                                aGCCGGGCTGGCGACCTCCCGTACCCCGGAAAGAACCATCACTGCTTTAAGGCATTTGTTGGAGGACCGGCGTAATCAGAAGCAACTACGCGATACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCATAAGCGCATCTTCTGt                           <  6:224447/149‑1 (MQ=255)
                                                                                                                aGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGCCTTCCAATACCGCAGAAGCGCATCTTCTGt                           >  4:247206/1‑149 (MQ=255)
                                                                                                                   ccggCCGCCGACCTCCCGTACCCCCGAATCACCCATCCCGGCTTTTCTGCATCTGGCGGCGGCCCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGc                        <  2:302037/147‑1 (MQ=255)
                                                                                                                      gCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCaa                      >  2:366733/1‑148 (MQ=255)
                                                                                                                        tGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAg                   >  5:322838/1‑149 (MQ=255)
                                                                                                                         gCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAgg                  <  1:366733/149‑1 (MQ=255)
                                                                                                                               cTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTg            <  4:71001/149‑1 (MQ=255)
                                                                                                                                 aCCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGcc          >  3:319287/1‑149 (MQ=255)
                                                                                                                                       ccccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAggg    >  6:318743/1‑149 (MQ=255)
                                                                                                                                        cccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAgggg   >  3:1507/1‑149 (MQ=255)
                                                                                                                                          cGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGc  >  3:301778/1‑148 (MQ=255)
                                                                                                                                          cGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGc  >  8:232764/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                              
CGCAAGTATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGC  >  NC_000913/2721100‑2721385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: