Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	455,600	C→A	G156G (GGC→GGA)	tig →	peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	455,600	0	C	A	100.0%	94.8 / NA	28	G156G (GGC→GGA)	tig	peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/15);  total (13/15)

AAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGCGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATC  >  NC_000913/455461‑455738                                                                                                                                            |                                                                                                                                           aaGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTg                                                                                                                                     >  5:42235/1‑147 (MQ=255)        tCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCagaa                                                                                                                             <  7:684/148‑1 (MQ=255)           ggAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGAcc                                                                                                                         >  4:73378/1‑149 (MQ=255)           ggAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGAcc                                                                                                                         <  1:3645/149‑1 (MQ=255)                                   aGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGtt                                                                                                 <  6:42235/149‑1 (MQ=255)                                        tCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTa                                                                                            <  2:96468/149‑1 (MQ=255)                                             gTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACgg                                                                                       >  3:27010/1‑149 (MQ=255)                                                       cGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTc                                                                             <  6:105208/149‑1 (MQ=255)                                                             ttGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGc                                                                       >  7:122777/1‑149 (MQ=255)                                                                aaGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCgg                                                                     <  2:33525/148‑1 (MQ=255)                                                                aaGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGt                                                                    <  3:73378/149‑1 (MQ=255)                                                                     aCCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGGCGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGc                                                               <  5:16005/149‑1 (MQ=255)                                                                     aCCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGc                                                               <  5:23140/149‑1 (MQ=255)                                                                             tGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGAtt                                                       >  6:65409/1‑149 (MQ=255)                                                                               aCGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTc                                                     <  8:122777/149‑1 (MQ=255)                                                                               aCGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTc                                                     <  4:27010/149‑1 (MQ=255)                                                                                                            aaaCAGCAGGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGAt                        >  7:112835/1‑149 (MQ=255)                                                                                                                agcagGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCg                    >  7:4728/1‑149 (MQ=255)                                                                                                                agcagGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCg                    >  1:100724/1‑149 (MQ=255)                                                                                                                agcagGCGACCTGGAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCg                    >  1:21954/1‑149 (MQ=255)                                                                                                                            ggAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCg                    >  2:3012/1‑137 (MQ=255)                                                                                                                            ggAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCg                    <  1:3012/137‑1 (MQ=255)                                                                                                                            ggAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGAc        >  3:65210/1‑149 (MQ=255)                                                                                                                            ggAAAGAAAAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGAc        >  2:77094/1‑149 (MQ=255)                                                                                                                              aaagaaaAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACgg      <  4:65210/149‑1 (MQ=255)                                                                                                                              aaagaaaAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACgg      <  2:21954/149‑1 (MQ=255)                                                                                                                              aaagaaaAAGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACgg      <  1:77094/149‑1 (MQ=255)                                                                                                                                   aaaaGACGGAGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATc  >  8:75712/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                           AAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCGTAAACAGCAGGCGACCTGGAAAGAAAAAGACGGCGCTGTTGAAGCAGAAGACCGCGTAACCATCGACTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATC  >  NC_000913/455461‑455738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑