Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	410,927	Δ1 bp	5.7%	coding (784/909 nt)	mak →	manno(fructo)kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	410,927	0	C	.	5.7%	233.8 / 6.7	70	coding (784/909 nt)	mak	manno(fructo)kinase
Reads supporting (aligned to +/- strand):  ref base C (34/32);  new base . (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCAT  >  NC_000913/410784‑411074                                                                                                                                                 |                                                                                                                                                    ttGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGACGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACg                                                                                                                                                >  2:224350/1‑149 (MQ=255)    aagtaAGCGATCCGGTAGCGGATCTGGCATTGCGTCGGTAAGAGCTGCGGCTGGCAAAATCGCTGGCAGATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGt                                                                                                                                              <  6:38705/148‑1 (MQ=255)    aagaaTGCGATCCGGTAGCGTAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACAGtt                                                                                                                                             <  4:205931/149‑1 (MQ=255)     agaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTg                                                                                                                                            <  2:230446/149‑1 (MQ=255)     agaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTg                                                                                                                                            <  1:280554/149‑1 (MQ=255)      gaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTTGATAT‑AAACGGtcta                                                                                                                                           <  6:351015/149‑4 (MQ=255)      gaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACgg                                                                                                                                               <  6:222987/145‑1 (MQ=255)      gaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACgg                                                                                                                                               >  5:222987/1‑145 (MQ=255)      gaaAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCAGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTgg                                                                                                                                           >  4:168831/1‑149 (MQ=255)          gCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAg                                                                                                                                       >  5:272870/1‑149 (MQ=255)              tCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGa                                                                                                                                   <  3:319628/149‑1 (MQ=255)              tCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGa                                                                                                                                   <  4:314381/149‑1 (MQ=255)                cGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGtt                                                                                                                                     >  7:185228/1‑145 (MQ=255)                cGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGtt                                                                                                                                     <  8:185228/145‑1 (MQ=255)                         aagtGGCATTGCGTCGCTACGAGCTGCGGATGGCAAAATCGCTGGCACATGTCTTGAATATTATCGATCCGGATGTGATTGTCCTGGGCGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAAttt                                                                                                                        <  5:188242/146‑1 (MQ=255)                         aaCTGGCATCGCGTCGCTACGAGCCGCGTCTGGCAAAATCGCTGGCCCCTGTCGCGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGGTGATTAAACAAttt                                                                                                                        <  4:47220/149‑1 (MQ=255)                            tGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTc                                                                                                                     >  2:85671/1‑149 (MQ=255)                                        gCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGa                                                                                                          <  2:96498/148‑1 (MQ=255)                                            cGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAAtgt                                                                                                      <  6:272870/148‑1 (MQ=255)                                               gCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGaaa                                                                                                  <  1:85671/149‑1 (MQ=255)                                                     gCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCgg                                                                                            <  3:168831/149‑1 (MQ=255)                                                      cTGGCAAAATCGCTGGCACATGACGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGt                                                                                           <  1:224350/149‑1 (MQ=255)                                                         gCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGTGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGACGGTGCg                                                                                        >  2:219812/1‑149 (MQ=255)                                                                   tGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAg                                                                              >  7:282809/1‑149 (MQ=255)                                                                         aTGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTc                                                                                                                  <  5:84978/107‑1 (MQ=255)                                                                         aTGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTc                                                                                                                  >  6:84978/1‑107 (MQ=255)                                                                           ggcgggAATATTCTCGATCCGGATGTGATTGCCCTGGGGGGCGGGAGGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACg                                                                          <  3:155412/140‑1 (MQ=255)                                                                           gTCGTGAATATTCTCGATCCGGATTTCATTGTACTCGGGGGAGGCATGAGCAATATACAACCATT‑ATATCAAACGTTATCGCACTAGATTAAACAATTTGTCTTCTGCTCCGAATCTTAAAAGCAGGTCCTTAAGACGAAGCACg                                                                          >  4:155412/1‑145 (MQ=255)                                                                              gTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAAt                                                                                                        >  7:287609/1‑112 (MQ=255)                                                                              gTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAAt                                                                                                        <  8:287609/112‑1 (MQ=255)                                                                                gAATATTCTCGCTCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGaaa                                                                                                  >  5:148436/1‑116 (MQ=255)                                                                                gAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGaaa                                                                                                  <  6:148436/116‑1 (MQ=255)                                                                                 aaTATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAATCGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAg                                                                >  8:301401/1‑149 (MQ=255)                                                                                   tatTCTCGATCCGGATGTGATTGTCGTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAgcg                                                              >  5:124074/1‑149 (MQ=255)                                                                                        tcGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTa                                                         >  1:153230/1‑149 (MQ=255)                                                                                        tcGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTa                                                         >  4:196743/1‑149 (MQ=255)                                                                                        tcGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTa                                                         >  3:65914/1‑149 (MQ=255)                                                                                        tcGATCCGGATGTGATTGTCCTGGGGGGCGGCATGAGCAATGTAGACCGTTT‑ATATCAAACGGTAGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCCGTGCGTAAGGCGAAGCACGTTGATTCCAGCGTCGTa                                                         >  7:299719/1‑149 (MQ=255)                                                                                           aTCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTAtgc                                                      >  3:100079/1‑149 (MQ=255)                                                                                           aTCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTAcgc                                                      <  8:282809/149‑1 (MQ=255)                                                                                              cGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACgcggc                                                   >  1:216195/1‑149 (MQ=255)                                                                                                gATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGgcgc                                                 >  7:31558/1‑149 (MQ=255)                                                                                                      aTTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAATCGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGt                                            <  7:301401/148‑1 (MQ=255)                                                                                                      aTTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTg                                           >  6:111329/1‑149 (MQ=255)                                                                                                      aTTGGCCTGGGGGGCGGGATTAGCAATGTAGACCGTTT‑ATATCAATCGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGt                                            <  8:31558/148‑1 (MQ=255)                                                                                                          tCCTGGGGGGCGGGATGAGCAATGAAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAAtgt                                                                                                      >  7:177454/1‑86 (MQ=255)                                                                                                          tCCTGGGGGGCGGGATGAGCAATGAAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAAtgt                                                                                                      <  8:177454/86‑1 (MQ=255)                                                                                                              ggggggCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGc                                   >  5:354676/1‑149 (MQ=255)                                                                                                                   gCGGGATGTGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACaa                              <  1:219812/149‑1 (MQ=255)                                                                                                                   gCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCcac                                >  4:28082/1‑147 (MQ=255)                                                                                                                   gCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCcac                                >  7:117727/1‑147 (MQ=255)                                                                                                                   gCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACaa                              <  1:363258/149‑1 (MQ=255)                                                                                                                       gATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAagag                           >  7:105303/1‑148 (MQ=255)                                                                                                                            gCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTaaaa                      >  5:302743/1‑148 (MQ=255)                                                                                                                            gCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTaaaa                      >  5:297582/1‑148 (MQ=255)                                                                                                                             cAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTaaaaaa                    >  5:156184/1‑149 (MQ=255)                                                                                                                                gcccaaCCGTTTGATAT‑AAACGGTCGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACg                  >  2:354535/6‑148 (MQ=255)                                                                                                                                  tAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTa                <  5:111329/148‑1 (MQ=255)                                                                                                                                  tAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAg               <  6:124074/149‑1 (MQ=255)                                                                                                                                  ccaaCCGTTTGATAT‑AAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAg               >  1:187997/4‑149 (MQ=255)                                                                                                                                  ccaaCCGTTTGATAT‑AAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAg               <  2:187997/146‑1 (MQ=255)                                                                                                                                    gACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAgg              >  2:328516/1‑148 (MQ=255)                                                                                                                                    gACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAgg              >  7:263393/1‑148 (MQ=255)                                                                                                                                       cGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAAt          <  3:196743/149‑1 (MQ=255)                                                                                                                                        gTTT‑ATAGCAAACGGTTGGGCAGTTGATTAAACAATTTTTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCAGGGTGATTGCAGCGGCGTACGCGGCGCTGGGTGGTTATGGCCACAAGAGTAAAATACGTAGGCAAtt         <  8:299719/149‑1 (MQ=255)                                                                                                                                         ttt‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAg               <  3:154131/142‑1 (MQ=255)                                                                                                                                         ttt‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAg               >  4:154131/1‑142 (MQ=255)                                                                                                                                              tatCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGgcg     <  2:216195/148‑1 (MQ=255)                                                                                                                                                tCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGcat  >  1:278574/1‑149 (MQ=255)                                                                                                                                                tCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGca   <  6:156184/148‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                    TTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGGATGTGATTGTCCTGGGGGGCGGGATGAGCAATGTAGACCGTTT‑ATATCAAACGGTTGGGCAGTTGATTAAACAATTTGTCTTCGGCGGCGAATGTGAAACGCCGGTGCGTAAGGCGAAGCACGGTGATTCCAGCGGCGTACGCGGCGCTGCGTGGTTATGGCCACAAGAGTAAAAAACGTAGGCAATTGGCGCAT  >  NC_000913/410784‑411074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑