Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 600,300 Δ1 bp 6.2% coding (1587/3144 nt) cusA → copper/silver efflux system, membrane component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913600,3000G. 6.2% 208.6 / 7.5 64coding (1587/3144 nt)cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base G (28/30);  new base . (2/2);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGC  >  NC_000913/600158‑600442
                                                                                                                                              |                                                                                                                                              
aaaCGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGaaa                                                                                                                                          <  8:241417/149‑1 (MQ=255)
     tATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACt                                                                                                                                     >  1:73161/1‑149 (MQ=255)
              gCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGc                                                                                                                             <  3:341078/148‑1 (MQ=255)
              gCGGGGGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTCCTGCACTGGc                                                                                                                             <  6:312465/148‑1 (MQ=255)
                      ggcgCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCa                                                                                                                    <  7:13934/149‑1 (MQ=255)
                      ggcgCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCa                                                                                                                    >  4:214098/1‑149 (MQ=255)
                         gctgctGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCTCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACgc                                                                                                                 <  5:206153/149‑1 (MQ=255)
                                gCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCtggt                                                                                                          <  3:214098/149‑1 (MQ=255)
                                gCGAGCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCTTTTCTAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTAGTGTTTATCATCCTCTGTTTTTGTAAGTTCTTCACTTGCCGTTTTCCTCGctgctg                                                                                                            <  2:73161/147‑1 (MQ=255)
                                  gATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACgc                                                                                                                 <  4:287535/140‑1 (MQ=255)
                                  gATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACgc                                                                                                                 >  3:287535/1‑140 (MQ=255)
                                         gTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCt                                                                                                 >  8:48276/1‑149 (MQ=255)
                                            aTCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCttt                                                                                               <  7:48276/148‑1 (MQ=255)
                                             tCCCGATCCTGATGGGCTACTGGATCCGTGGCATAATTCCGCCGGAAAGCAGTAACCCGCTCAGTCGCTTTTTGATTCGTGGTTATCATCCGCTGTTGCTGCAAGTACTGCGCTGGCCGATAACCCCGGTGCGGGTGGCGGCGGTTTCg                                                                                             >  8:71888/1‑149 (MQ=255)
                                               ccGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGt                                                                                           >  6:344536/1‑149 (MQ=255)
                                                cGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTg                                                                                          <  6:107798/149‑1 (MQ=255)
                                                      tGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCa                                                                                                                    >  5:311894/1‑117 (MQ=255)
                                                      tGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCa                                                                                                                    <  6:311894/117‑1 (MQ=255)
                                                      tGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACg                                                                                    >  3:164937/1‑149 (MQ=255)
                                                             cTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTtctcg                                                                             >  2:243585/1‑148 (MQ=255)
                                                               aCTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCgg                                                                                            >  8:81525/1‑132 (MQ=255)
                                                               aCTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCgg                                                                                            <  7:81525/132‑1 (MQ=255)
                                                                   gATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGc                                                                       >  5:349013/1‑149 (MQ=255)
                                                                         tGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTAc                                                                                                                                      >  2:33999/1‑80 (MQ=255)
                                                                         tGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTAc                                                                                                                                      <  1:33999/80‑1 (MQ=255)
                                                                          ggCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTctct                                                                             <  6:238063/136‑1 (MQ=255)
                                                                          ggCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTctct                                                                             >  5:238063/1‑136 (MQ=255)
                                                                           gCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATa                                                                 >  4:322767/1‑147 (MQ=255)
                                                                                 ttCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGc                                                         >  7:189826/1‑149 (MQ=255)
                                                                                     gccgGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGc                                                                                                                             >  2:295703/1‑77 (MQ=255)
                                                                                     gccgGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGc                                                                                                                             <  1:295703/77‑1 (MQ=255)
                                                                                       cgGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGaa                                                   <  4:251864/149‑1 (MQ=255)
                                                                                             gCAGTAACCCGCTCAATCGCTTTTTGATTCGTGGTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCCCGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGGTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTa                                             <  1:14072/149‑1 (MQ=255)
                                                                                              cAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTAc                                            >  1:9205/1‑149 (MQ=255)
                                                                                                gTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCg                                          <  8:140581/149‑1 (MQ=255)
                                                                                                   aCCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCg                                          >  1:325067/1‑146 (MQ=255)
                                                                                                    cccGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCg                                          >  1:12245/1‑145 (MQ=255)
                                                                                                     ccGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCtgg                                                                                                           <  8:133925/79‑1 (MQ=255)
                                                                                                     ccGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCtgg                                                                                                           >  7:133925/1‑79 (MQ=255)
                                                                                                         tCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAAt                                 >  4:300919/1‑149 (MQ=255)
                                                                                                           aaTCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATg                                >  2:238053/1‑148 (MQ=255)
                                                                                                           aaTCGCTTTTTGATTCGTGTTTATCATCCGCTGTTCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATg                                >  3:307430/1‑147 (MQ=255)
                                                                                                           aaTCGCTTTTTGATTCGTGTTTATCATCCGCTGTTCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATg                                >  3:312604/1‑147 (MQ=255)
                                                                                                            aTCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATg                                >  5:189500/1‑147 (MQ=255)
                                                                                                               gCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGc                           <  6:189500/149‑1 (MQ=255)
                                                                                                               gCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTGCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAgg                            <  1:243585/148‑1 (MQ=255)
                                                                                                                cTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGc                           >  1:143674/1‑148 (MQ=255)
                                                                                                                 tttttGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGAGGGGAATTTTTACCGCAGATCAATGAAGGCGa                         >  4:69545/1‑149 (MQ=255)
                                                                                                                   tttGATTCGTGTTTATCATCCGCTGTTCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGAc                        <  4:312604/147‑1 (MQ=255)
                                                                                                                   tttGATTCGTGTTTATCATCCGCTGTTCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGAc                        <  4:307430/147‑1 (MQ=255)
                                                                                                                      gATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGa                                                                                      <  4:4900/83‑1 (MQ=255)
                                                                                                                      gATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGa                                                                                      >  3:4900/1‑83 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACTCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  5:344536/149‑1 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  1:238053/149‑1 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  3:322767/149‑1 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  2:325067/149‑1 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  7:71888/149‑1 (MQ=255)
                                                                                                                       aTTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  2:9205/149‑1 (MQ=255)
                                                                                                                          cGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTtgttgt                 <  2:12245/148‑1 (MQ=255)
                                                                                                                          cGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGAGGGGAATTTTTACCGCAGATCAATGAAGGCGACTtgttgt                 <  3:69545/148‑1 (MQ=255)
                                                                                                                           gtgtTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   <  5:227956/145‑1 (MQ=255)
                                                                                                                           gtgtTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACttgtt                   >  6:227956/1‑145 (MQ=255)
                                                                                                                                    atcCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATTAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATAGGCCagc       >  6:17124/1‑146 (MQ=255)
                                                                                                                                        gCTGTTTCTGAAAGTACTGCACTGGCCGAAATCCACGCTTCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTTGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGc  <  6:349013/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                              
AAACGTATGCGATGGCGGGTGCGGCGCTGCTGGCGATCGTAGTGATCCCGATCCTGATGGGCTACTGGATCCGTGGCAAAATTCCGCCGGAAAGCAGTAACCCGCTCAATCGCTTTTTGATTCGTGTTTATCATCCGCTGTTGCTGAAAGTACTGCACTGGCCGAAAACCACGCTGCTGGTGGCGGCGCTTTCGGTGCTGACGGTTCTCTGGCCGCTCAATAAAGTTGGCGGGGAATTTTTACCGCAGATCAATGAAGGCGACTTGTTGTATATGCCATCGACGC  >  NC_000913/600158‑600442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: