New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4356370 =172 (2.180)4 (0.050) 4/250 NT 2.3% coding (42/1458 nt) dtpC dipeptide and tripeptide permease
?NC_000913 4356389 = 174 (2.230)coding (23/1458 nt) dtpC dipeptide and tripeptide permease
Rejected: Frequency below/above cutoff threshold.

TGATGGCCGCTATACCGTTAAGGTATTGAAAGAAGAAAGCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4356518‑4356370
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGA  >  NC_000913/4356389‑4356426
                                                                                                                                                                                           
TGATGGCCGCTATACCGTTAAGGTATTGAAAGAAGAAAGCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG                                        >  5:50321/1‑149
  ATGGCCGCTATACCGTTAAGGTATTGAAAGAAGAAAGCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG                                        <  7:46385/147‑1
       CGCTATACCGTTAAGGTATTGAAAGAAGAAAGCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCACGATATAGTATATCGCGCGCGGCT                                 >  5:333043/1‑149
                                      GCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGA  <  6:333043/149‑1
                                                                        TTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG                                        >  3:347493/1‑77
                                                                        TTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG                                        <  4:347493/77‑1
                                                                                                                       TCACAGCCGCGCGCGATATACTATATCGTGCGCGGTTGTGAGGGTGTTTTCATGT               >  7:258837/1‑55
                                                                                                                       TCACAGCCGCGCGCGATATACTATATCGTGCGCGGTTGTGAGGGTGTTTTCATGT               <  8:258837/55‑1
                                                                                                                                                                                           
TGATGGCCGCTATACCGTTAAGGTATTGAAAGAAGAAAGCAAAAAATAATTAGCTCGTACAAGGGAAGTGGCTTGCCACTTCCCTTTTTTGCTCATAAGGAGAACACATGAAAACACCCTCACAGCCGCGCGCGATATACTATATCGTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4356518‑4356370
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gCGCGGCTGTGAGGGTGTTTTCATGTGTTCTCCTTATGA  >  NC_000913/4356389‑4356426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.